Beef cattle feeds and feeding - Khon Kaen University
Transcript of Beef cattle feeds and feeding - Khon Kaen University
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Beef cattle feeds and
feeding
Anusorn Cherdthong
AG177441 Applied Animal Nutrition
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More microbes
More Fiber and Starch Digestion
More VFA Production
More Energy +
More Microbial Protein
More
products
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Outline Digestive physiology
Rumen microorganisms
Feeds and utilization
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Protein
metabolism
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Carbohydrate
metabolism
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Ruminant
Most herbivores
Four compartment
stomach
Fore-gut fermentation vat
to digest plants
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Ruminant Nutrition and
Feeding:
Feeds Rumen Microorganisms
Productivity
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Ruminant Digestive Systems
Picture of digestive system of cow
Rectum
Anus
Colon
Cecum
Small IntestineRumen
Kidney
Pancreas
Liver Esophagus
Pharynx
Teeth
Tongue
Salivary
Gland
Reticulum
Omasum
Abomasum
Ruminant Digestive Systems
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Saliva of a cow contains no salivary
amylase
To digest the plant material,
herbivores need to depend on the
cellulase-secreting microorganism in
its digestive system
Ruminants..
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Saliva and rumen buffering capacity
NaHCO3
NaCl + H2 CO3weak acid
Na2CO3 + H2Oweak alkaline
+NaOH
+HCl
**Maintain rumen pH 6.5-7
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Stomach Compartments Rumen - fermentation vat
Reticulum - rumen’s
“assistant”
Omasum - dehydrator
Abomasum - glandular
stomach
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Rumen
Largest compartment
On left side of animal
Contains micro-organisms
Ferments cellulose
Absorbs VFA’s
Divided into chambers
Continually contracting
Contains papillae
Produces CO2 and CH4
pH close to neutral (6.5 - 7)
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Papillae in Rumen
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Papillae in Rumen
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A
B
C
A
B
A= reticulo-ruminal fold A = cardia
B=esophgus B= reticulo-amasal orifice
C= esophagus/reticular groove
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Reticulum
Smallest compartment
Lies close to the heart
Small sac - part of rumen body
Catches dense, heavy feed for
later rumination
Contracts for regurgitation
“Honeycomb” lining
Catches hardware and stores it
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Omasum - full
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Reticulum - full
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Reticulum - cleaned
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Omasum
Third compartment
Globe-shaped
Lining called “many plies”
Reduces feed particle size
Absorbs water and dries out
ingesta
Absorbs volatile fatty acids
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Abomasum
Final compartment
Tubular in design
“True” stomach (glandular)
Secretes HCl and enzymes for chemical
digestion
Reduces pH to 2.5
Dissolves minerals
Kills rumen bacteria
Breaks down proteins
Passes ingesta to small intestine
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Abomasum – inside view
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Ruminant Digestive Systems
◦ Small Intestine - where most of the food material
is absorbed into the bloodstream
Contains three sections:
duodenum
jejunum
ileum
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Rumen
microorganism
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Feeding the bugs,
feeding the cows
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Rumen as a Fermentation Vat : Rumen ecology : # Microorganisms
# Bacteria
# Protozoa
# Fungi
# pH
# Fermentation end-products
# VFA (C2, C3, C4)
# NH3-N
# rumen by-pass nutrients, protein# etc (Wanapat, 2000)
Feeds:
Roughages,
Concentrateetc
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2 DAYS 4 DAYS 8-10 DAYS 12-20 DAYS
CELLULOLYTIC
CONSORTIA
POLYCENTRIC
FUNGI
PROTOZOA
ZOOSPORE
MONOCENTRIC
FUNGI
UREA
OXYGEN
38 hr
Diagrammatic representation of the sequential development of
the microbial ecology of the newborn ruminant.
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Rumen Bacteria
• 1010 -1012 cells/ml rumen fluid
• Cellulolytic bacteria
• Amylolytic
• Proteolytic
• NH3-N utilizing• etc
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Groups of Bacteria in the
Rumen
1. Free-living in the liquid phase
2. Loosely associated with feed particles
3. Firmly adhered to feed particles
4. Associated with rumen epithelium
5. Attached to surface of protozoa and
fungi
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Distribution of rumen bacteria
75% = bacteria adhered to feeds
20% = flow in rumen fluid
5% = adhered to rumen epithelium
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Bacteria on feed particles
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Attachment of rumen bacteria on rice straw
of swamp buffalo
(Wanapat et al., 2000b)
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Rumen Protozoa
Ciliated protozoa
104-106 cells/ml
larger > bacteria
moves rapidly
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Holotrich- Subclass
◦ absorbs sugar
Entodinimorph (tuft)
◦ digest starch
• Stores surplus CHO
• Can not use NPN
• Engulf bacteria 200 cells/min
1% bacteria/min
• Defaunation vs Nondefaunation
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Rumen protozoa,
Holotrich sp.(Hungate, 1966)
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Rumen protozoa, Entodiniomorph sp. of
swamp buffalo (Wanapat et al., 2000b)
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Entodiniomorph
Diplodinium with
adherent
methanogenic bacteria
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Symbiosis of bacteria protozoa and fungal zoospores
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Rumen Fungi
• ~ 8% of total rumen
microbes
• ~ 20 genera
• low in number
• digest fiber with bacteria
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Life cycle of rumen fungi
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Rumen fungi on fiber digestion
A germinated zoospore in a stoma of guinea grass from rumen
of buffaloes
A = 30 min after incubation (Bar = 5 micron) B = 24 hr after incubation (Bar = 25 micron)Source : Ho et al. (1988)
A B
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Rumen fungus of swamp buffalo,
Anaeromyces sp. with acuminate apex
(Wanapat et al., 2000b)
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A = Rumen fungal sporangium with flagellae
B = Rumen fungal rhizoid with penetrated
appressorium of swamp buffalo
(Wanapat et al., 2000b)
A B
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Feeds and feed utilization
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Feeds for Beef Cattle
Roughage Concentrate
Pasture Other forages
Crop residues ProteinEnergy
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Fermentation by-productsCarbohydrate Protein
Volatile fatty acids
-Acetate (C2)
-Propionate (C3)-Butyrate (C4)
Microbial protein
CO2, CH4 NH3
Microbial cell CO2, CH4
Starch Amino acid/protein
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Energy Supply to Ruminants
Contribution of the microbes to the
symbiotic relationship:
VFA 70%
Microbial cells 10%
Digestible unfermented feed 20%
Concentration of VFA in the rumen 50 to 125
uM/ml
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Amino Acid Supply to Ruminants
Contribution of the microbes to the symbiotic relationship
Protein in microbial mass 65%
Undegraded feed proteins 30%
Recycled endogenous proteins 5%
Amino acid balance of microbial mass is
superior to that from undegraded feed
proteins when corn-based diets are fed.
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Interaction between microbes on SC digestion
Entodiniomorphs
*Polyplastron, *Eudiplodinium
*Neocallimastic*Piromyces
*Opinomyces
*Anaeromyces
*Caecomyces
exo-1,4-B-glucanase/
cellulase
endo-1,4-B-glucanase/
exo -1,4-B-glucanase
endo-1,4-B-D-glucanase/
exo-1,4-B-D-glucanase
Structural carbohydrate
VFA
Cellulolytic
bacteria
(Hungate, 1996; Ushida and Jouanu, 1994; Ho and Abdullah, 1999)
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Holotrich Entodiniomorph
*Neocallimastic*Piromyces
*Opinomyces
*Anaeromyces
*Caecomyces
Peptidase Protease, Peptidase Metalloprotease
Zn+ cofactor
Protein, NPN
NH3 and AA
Proteolyticbacteria
Interaction between microbes on Protein digestion
(Hungate, 1996; Ushida and Jouanu, 1994; Ho and Abdullah, 1999)
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Protein
Large Peptides
Small Peptides
Amino Acids
Amino acids
Microbial
Protein
Carbohydrates
Oligosaccharides
Disaccharides
Monosaccharides
Monosaccharides
Volatile Fatty acids
NH3
Cell Membrane
NH3
ADP
ATP
NAD
NADH2
ATP
ADPATP
ADPNAD
NADH2
NAD NADH2
H2 + CO2
CH4
Nocek and Russell (1988)
NH3
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Ruminal CH4 Production
18% from livestock (FAO, 2006)
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Protein
metabolism
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Carbohydrate
metabolism
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Thank you
very much