Because science never stands still. · Because science never stands still. ... tual platform •...

14
Because science never stands still. Introducing the FREE Mobile App from Science. See what it can do for you. Now you can access Science from anywhere. Our new app lets you: Read summaries and abstracts from Science, Science Translational Medicine, and Science Signaling. E-mail yourself links to full text. Stay updated with ScienceNOW, our free daily news service. Search job openings and tap other resources at Science Careers. Access the Science weekly podcast and other multimedia. Store content for reading without wi-fi access. Get it for your iPhone or iPod Touch. Get it for FREE. Get it today. To download the free app visit the iTunes App Store or log on to content.aaas.org/mobile

Transcript of Because science never stands still. · Because science never stands still. ... tual platform •...

Page 1: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Because science never stands still.Introducing the FREE Mobile App from Science. See what it can do for you.

Now you can access Science from anywhere. Our new app lets you:

• Read summaries and abstracts from Science,

Science Translational Medicine, and Science Signaling.

• E-mail yourself links to full text.

• Stay updated with ScienceNOW, our free daily news service.

• Search job openings and tap other resources at Science Careers.

• Access the Science weekly podcast and other multimedia.

• Store content for reading without wi-fi access.

Get it for your iPhone or iPod Touch. Get it for FREE. Get it today.

To download the free app visit the iTunes App Store or log on tocontent.aaas.org/mobile

Page 2: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

www.abcam.com

Abcam Inc.1 Kendall Square, Ste 341

Cambridge, MA 02139-1517

USA

Tel: 1-617-225-2272

Toll free: 1-888-77-ABCAM

Toll free Fax: 1-866-739-9884

Emission spectra of DyLight®

fluorochromes.

Line colors represent the

approximate visible colors of the

wavelength maxima.

What colorwill help you today?Abcam offers hundreds of DyLight® conjugated secondary

antibodies that complement our products in a wide range

of applications.

• Bright fluorescence

• Excellent photostability

• Stable at pH4 - 9

• Pre-adsorbed formats

For all your secondary antibody needs visit:

www.abcam.com/secondary_antibody

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Page 3: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

GE,im

ag

ina

tion

at

wo

rk,an

dG

Em

on

og

ram

are

trad

ema

rkso

fG

enera

lElectricC

om

pa

ny.

ÄK

TA,A

xichro

m,B

ioP

rocess,C

ap

to,H

iScale,H

iScreen,M

ab

Select,an

dU

NIC

OR

Na

retra

dem

arks

of

GE

Hea

lthca

reco

mp

an

ies.©

20

10

Gen

eralElectric

Co

mp

an

y–

Allrig

hts

reserved.

Firstp

ub

lished

Sept.2

01

0G

EH

ealth

care

Bio

-Sciences

AB

,Bjö

rkga

tan

30

,751

84

Up

psa

la,Sw

eden

GE0

9-1

0

Inspired toÄccelerate scale-up

GE HealthcareLife Sciences

Inspired by your need for fast and predictable scale-up, we introduce ÄKTA™ avant 150,the latest development in our ÄKTA avant series.

Optimize your process with ÄKTA avant 25 and then scale-up seamlessly to ÄKTAavant 150 by automatically converting your methods using UNICORN™ 6.1 software.

ÄKTA avant is designed to fully exploit the advantages of our modern BioProcess™

media like MabSelect™ and Capto™.

Dedicated column formats ensure seamless scalability from process developmentto manufacturing – from prepacked HiScreen™ columns to HiScale™ to AxiChrom™

with Intelligent Packing.

Want to know more? Register today to receive a copy of the ÄKTA avant 150 Data File.www.gelifesciences.com/pr-avant

Page 4: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Sample & Assay Technologies

AD

NA

Sam

ple

Portf0

910S1W

W

Any sample, any application — no limits

Maximize success

with QIAGEN sample technologies

■ Innovative, room-temperature sample collection and stabilization

■ DNA, RNA, and protein purification from any sample

■ Hands-free automated sample preparation

■ Reliable genetic, epigenetic, and gene expression analysis from FFPE samples

■ Whole genome and transcriptome amplification to overcome sample limitations

Contact QIAGEN today or visit www.qiagen.com/sample-technologies

Page 5: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Professor Tanke, why is curiosity

a building block of life?

Hans Tanke, a researcher and pioneer of digital fluorescence microscopy, is driven by

passionate curiosity. He is Head of the Department of Molecular Cell Biology at Leiden

University Medical Center, Netherlands, where he gives young scientists creative

freedom: this enables them to develop the ethically responsible dedication with which

he himself keeps tracking down the building blocks of life.

www.leica-microsystems.com

Dedication to Science

How is the light wave-

length of knowledge

calculated? Leica Micro-

systems has mapped its

corporate values.

For more information,

visit our website.

Page 6: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

CLONING & MAPPINGDNA AMPLIFICATION

& PCRRNA ANALYSIS

PROTEIN EXPRESSION &

ANALYSIS

GENE EXPRESSION

& CELLULAR ANALYSIS

Sample Preparation for Next Gen SequencingIntroducing NEBNext™, a series of highly pure reagents that facilitate sample preparation for downstream applications such

as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust

reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

www.neb.com

STUNNING QUALITY

Sequencing coverage map of the E. coli genome after using

NEBNext™ Sample Prep Reagent Set 1 for Sample Preparation

E. coli strain MG1655 gDNA was prepared with NEBNext Sample Prep Reagent Set I and sequenced on an Illumina Genome

Analyzer II.

For more information about NEBNext, including customized solutions, please contact [email protected].

160

140

120

100

80

0 1x106 2x106 3x106 4x106

SequenceCoverage

Genome Position

Page 7: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Robert H. WeisbergDistinguished University Professor

of Physical Oceanography,

University of South Florida

CONFERENCE CO-CHAIRS

CALL FOR ABSTRACTS

William T. HogarthDean, USF College of Marine Science

Acting Director Florida Institute

of Oceanography

Michael P. CrosbySenior Vice President for

Research, Mote Marine

Laboratory

Page 8: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Over

30 years of

experience

Performing for excellence

Serial dispensing at its best: Repeater®

The Eppendorf Repeater handheld dispensing system

offers an outstanding range of benefits:

Together with Combitips plus®, both the electronic and

manual hand dispenser offer automatic tip recognition

combined with an automatic volume calculation.

The positive displacement system ensures high-precision

dispensing for all kinds of liquids including volatile and

viscous solutions. 9 different sizes of tips offer a volume-

range from 1 µL – 50 mL.

Easy volume setting

‡ Repeater plus: 1 µL – 10 mL

‡ Repeater stream/Xstream: 1 µL – 50 mL

Up to 100 dispensing steps without stopping

‡ Repeater plus:

20 different adjustable volumes per tip

‡ Repeater stream/Xstream:

1,000 different adjustable volumes per tip

For more information visit www.eppendorf.com/mpplus

or www.eppendorf.com/mpxstream

‡ Electronic

dispensing

‡ Positive

displacement tips

‡ Manual

dispensing

ep

pend

orf

®,R

ep

eate

r®and

Co

mb

itip

sp

lus

®are

reg

iste

red

trad

em

ark

so

fE

pp

en

do

rfA

G.

All

rig

hts

reserv

ed

,in

cl.

gra

phic

sand

imag

es.

Co

pyrig

ht

©2010

by

Ep

pend

orf

AG

.

Your local distributor www.eppendorf.com/worldwide · Application Support E-mail: [email protected]

Eppendorf AG · Hamburg · Germany · Tel: +49 40 538 01-0 · Eppendorf North America, Inc. · USA · Tel: +1 800 645 3050 menu option 2

Page 9: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

FOR RESEARCH, FORENSIC OR PATERNITY USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.© 2010 Life Technologies Corporation.

Who knows what you’ll discover next?

With a streamlined workflow, one-button protocol feature, and intuitive software, the new ViiA™ 7

Real-Time PCR System is faster to set up, easier to run, and more convenient to automate

than any other qPCR platform. And with the new OptiFlex™ optical system, you’ll have greater

dye flexibility for multiplexing than ever before. The end result is high-accuracy data with

minimal distraction, which means you can stay focused on what matters most—your research.

For more information, visit www.appliedbiosystems.com/viia7

First you’ll discover how easyhigh-productivity qPCR can be.

Optimized for TaqMan®

Array Micro Fluidic Cardsand reagents.

Page 10: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

online • forum •mass media • public policy • interactive • society • science fair • exhibit • discussion • invworkshop • interview • television • �lm • public • radio • blog • festival • hands-on •m�se�m • brainstorm

motivate •mutual learning • listen • build • experiment • contribute • learn • active • virtual platform •Web2.0deliberate • participation • dialogue • engage • informal science ed�cation • p�blic o�treach • social mediascience café • online • forum • mass media • public policy • interactive • society • science fair • exhibit

discussion • involve • workshop • interview • television • �lm • public • radio • blog • festival • hands-onmuseum • brainstorm •motivate •mutual learning • listen • build • experiment • contribute • learn • activ

tual platform • Web2.0 • deliberate • participation • dialogue • engage • informal science educationpublic outreach • social media • science café • online • forum • mass media • public policy • interactivsociety • science fair • exhibit • discussion • involve • workshop • interview • television • �lm • publicadio • blog • festival • hands-on • museum • brainstorm • motivate • mutual learning • listen • buildexperiment • contribute • learn • active • virtual platform • Web2.0 • deliberate • participation • dialogueengage • informal science education • public outreach • social media • science café • online • forum

mass media • public policy • interactive • society • science fair • exhibit • discussion • involve • workshopinterview • television • �lm • public • radio • blog • festival • hands-on • museum • brainstorm • motivmutual learning • listen • build • experiment • contribute • learn • active • virtual platform • Web2.0

deliberate • participation • dialogue • engage • informal science education • p�blic o�treach • socialmedia • science café • online • forum • mass media • public policy • interactive • society • science fairexhibit • discussion • involve • workshop • interview • television • �lm • public • radio • blog • festivhands-on • museum • brainstorm • motivate • mutual learning • listen • build • experiment • contributelearn • active • virtual platform • Web2.0 • deliberate • participation • dialogue • engage • informal scienceed�cation • public outreach • social media • science café • online • forum • mass media • public policyinteractive • society • science fair • exhibit • discussion • involve • workshop • interview • television • �lmpublic • radio • blog • festival • hands-on •museum • brainstorm •motivate •mutual learning • listen • buildexperiment • contribute • learn • active • virtual platform •Web2.0 • deliberate • participation • dialogue

engage • informal science education • p�blic o�treach • social media • science café • online • forum • massmedia • public policy • interactive • society • science fair • exhibit • discussion • involve •workshop • interviewtelevision • �lm • p�blic • radio • blog • festival • hands-on • museum • brainstorm • motivate • mutual

learning • listen • build • experiment • contribute • learn • active • virt�al platform • Web2.0 • deliberateticipation • dialogue • engage • informal science education • public outreach • social media • science café

online • for�m •massmedia • public policy • interactive • society • participation • dialogue • engage • informalscience education • p�blic o�treach • social media • science café • online • forum •mass media • public policyinteractive • society • science fair • exhibit • discussion • involve • workshop • interview • television • �lm

p�blic • radio • blog • festival • hands-on •museum • brainstorm •motivate •mutual learning • listen • buildexperiment • contribute • learn • active • virt�al platform • Web2.0 • deliberate • participation • dialogueengage • informal science education • public outreach • social media • science café • online • for�m • mass

media • public policy • interactive • society • participation • dialogue • engage • informal science educationp�blic o�treach • social media • science café • online • forum • mass media • public policy • interactiv

society • science fair • exhibit • discussion • involve • workshop • interview • television • �lm • p�blic • rablog • festival • hands-on • museum • brainstorm • motivate • mutual learning • listen • build • experimentcontribute • learn • active • virt�al platform • Web2.0 • deliberate • participation • dialogue • engage

Nominations are open now thro�gh October 15 for the AAASEarl� Career Award for P�blic Engagement with Science.With this new award, AAAS will recognize early-careerscientists and engineers who demonstrate excellence intheir contribution to public engagement with scienceactivities. The award recipient will receive a monetary prizeof $5,000, a commemorative plaque, and complimentaryregistration and reimbursement of travel expenses to the2011 AAAS Annual Meeting inWashington, D.C.

For eligibility information and instructions on submittingnominations, visit http://www.aaas.org/go/PESaward.

AAAS Earl� Career Award forP�blic Engagement with Science

... how do yOu engage?

Page 11: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Cambridge Healthtech Institute’s 10th Annual

The Protein ScienceWeek

JANUARY 10-14, 2011

Hotel Del Coronado • San Diego, CA

chi-PepTalk.comCambridge Healthtech Ins�tute, 250 First Avenue, Suite 300, Needham, Massachuse�s 02494Telephone: 781-972-5400 or toll-free in the U.S. 888-999-6288Fax: 781-972-5425 • www.healthtech.com

Be sure to mention

Keycode L35 AD

when Registering!

C H I

PIPELINE 4HIGHER-THROUGHPUT PROTEIN EXPRESSION

Targeting Genes, Engineering Vectors, Designing Constructs, and Optimizing Clones

Choosing, Designing, and Optimizing Hosts and Platforms

Overcoming Protein Expression Challenges with Solutions

Membrane Proteins

PIPELINE 3BIOTHERAPEUTICS

Recombinant Protein Therapeutics

Antibodies for the 21st Century

Antibody Drug Products

PIPELINE 1FORMULATING BIOLOGICS

Optimizing Biologics Formulation Development

Protein-Device Combinations

Lyophilization, Spray Drying & Emerging Drying Technologies

Protein Aggregation and Emerging Analytical Tools

PIPELINE 2ANALYTICS OF PROCESS IMPROVEMENT

Protein Purification & Recovery

Protein Data Integration and Interrogation

Protein Computational Tools

FOCUS ON CAREERS

Turn to page 249

or view online at sciencecareers.org/

career_magazine/business_office_features

A Science/AAAS Business Office Feature

Find out who made the list.

Page 12: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

247www.sciencemag.org/products SCIENCE VOL 330 8 OCTOBER 2010

NEW PRODUCTS

LIFE SCIENCE TECHNOLOGIES

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are

featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or

materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

AAAS/Science Business Office

PRESSURE-BASED DROPLET GENERATION SYSTEMThe Pressure-based Droplet Starter System is ideal for the initial

exploration of droplet microfl uidics. It provides a complete solution

containing all necessary pumps, connectors, and chips, enabling the

immediate production of highly monodispersed droplets from diam-

eters of 20 µm to 150 µm, with a diameter tolerance of ± 1 percent.

Operating over a wide pressure range of 0 bar to 10 bar, this Pres-

sure-based Droplet Starter System is extremely chemically resistant

and its modular design makes it easily expandable for future applica-

tion requirements. Providing a pulseless and stable liquid fl ow, this

cost-effective modular toolkit eliminates dead volume and sample

waste to effectively reduce associated reagent costs. In addition, the

ability to provide a quick liquid changeover signifi cantly increases the

throughput of any application. A lockable pressure chamber and an

innovative three-way chamber lid allow simultaneous pumping of up

to three liquids, making it ideal for entry-level experimentation.

Dolomite

For info: 617-803-6655 www.dolomite-microfl uidics.com

3D TISSUE GENERATIONReal Architecture for 3D Tissue (RAFT) has been developed for mak-

ing consistent multicellular 3D tissue constructs simply and rapidly,

in less than one hour. RAFT allows complex multilayer tissues to be

formed—with different cell types in each layer—and cells to be co-

cultured in a well controlled way. The resulting biomimetic tissues,

made from fi brillar collagen, are strong, transparent, and 50 µm to

100 µm thick. Additionally, a workstation and consumables has been

developed to automate and scale up this 3D tissue production pro-

cess, enabling up to 24 tissues to be made in parallel. Tissues are

made either in the wells of a 24-well plate; on permeable membrane

inserts for barrier assays; or cultured at an air/liquid interface, for

example to form stratifi ed epithelia. The tissue remains in the same

well from its creation until the end of the experiment and can be

analyzed using standard techniques.

The Automation Partnership

For info: +44-(0)-1763-227200 www.automationpartnership.com

Thermo Scientifi c

For info: 800-532-4752 www.thermoscientifi c.com/raman

REFRACTIVE INDEX DETECTIONThe Refractive Index (RI) detection instrument, the Optilab T-rEX,

provides full range of detection and sensitivity across the entire dy-

namic range, meaning there is no longer a need to compromise

one for the other. The range of the Optilab T-rEX enables the in-

strument to be used in almost any standard isocratic chromato-

graphic conditions and for many gradient applications. In addi-

tion, recent developments in heterojunction light emitting diodes

(LEDs) allow the T-rEX to have a light source 50 times brighter

than previous instrumentation. This superior light source results

in the detector array fi lling with light much faster than previously

possible. The new instrument also offers high speed data acquisi-

tion and includes an onboard computer, allowing the rapid scan-

ning of arrays, with 1 million measurements made every second.

The T-rEX also has a fl ow cell with a total volume of only 7.4 µL,

which is 25 percent less than the leading refractometers avail-

able today. This results in minimized band broadening and better

temperature stability.

Wyatt Technology

For info: 805-681-9009 www.wyatt.com

IgG FRAGMENTATION Immobilized papain, fi cin, and pepsin enzymes, provided in sizes of

5 mL resins, can be used to cleave immunoglobulin G (IgG) antibod-

ies near the hinge region. The immobilized papain, fi cin, and pepsin

offer the distinct advantage of eliminating enzyme contamination

of the created fragments. Papain produces two fragment antibody

binding (Fab) domains and one fragment crystallizable (Fc) domain.

Ficin cleaves IgG to create one Fab and one F(ab’)2 fragment. Pepsin

preserves the disulfi de bonds joining the heavy chains, which gener-

ates F(ab)2 fragments. Further separation of the digested products

can be performed with G-Biosciences’ Immobilized Protein A or by

ion exchange chromatography.

G-Biosciences

For info: 800-628-7730 www.gbiosciences.com

CARBON NANOMATERIAL CHARACTERIZATION

Designed to simplify the Raman technique for nonspecialist instru-

ment users, the new DXR Nanocarbon Analysis Packages enhance

productivity and provide accurate, rapid, and reproducible results.

These two packages incorporate rigorous automated calibration and

alignment routines, control of laser power, and sophisticated quality

checks to every spectrum collected. The DXR Nanocarbon Microanaly-

sis Package, featuring the DXR Raman Microscope, is a complete sys-

tem confi gured for microcharacterization. The DXR Nanocarbon Analy-

sis Package, which leverages the Thermo Scientifi c SmartRaman, is a

full system for bulk materials characterization. Both contain hardware,

software, and sampling accessories. Analysis packages are highly fl ex-

ible and can be customized to accommodate a wide variety of appli-

cations and sample forms covering important areas of carbon nano-

materials such as fundamental research, nanomaterial production, and

functionalization, as well as applied research on end-applications and

end-application production.

Page 13: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

Student PosterCompetitionAAAS Annual Meeting

Science Without Borders

Seize this opportunity to put your research in the spotlight

and discuss your work with leading scientists and

engineers from across the country and around the world.

All submissions will be peer reviewed. Presented posters

will be listed in the 2011 Annual Meeting Poster Guide.

Winners will be published in Science and online.

The competition is open to college undergraduate and

graduate students only. To learn more, including how you

can volunteer and register for free, visit “Poster

Submission” at www.aaas.org/meetings.

Winners receive a cash prize, award certificate, and

one-year AAASMembership with a subscription to Science!

17-21 February, Washington, D.C.

Submission Deadline:Monday, 25 October 2010

CALL FOR ENTRIES

Page 14: Because science never stands still. · Because science never stands still. ... tual platform • Web2.0 ... Operating over a wide pressure range of 0 bar to 10 bar, this Pres-sure-based

R&D Systems, Inc. www.RnDSystems.com

R&D Systems Europe, Ltd. www.RnDSystems.co.uk

R&D Systems China Co., Ltd. www.RnDSystemsChina.com.cn

For research use only. Not for use in diagnostic procedures.

Cancer

Development

Endocrinology

Glycobiology

Immunology

Neuroscience

Proteases

Signal Transduction

Stem Cells

For more information visit our website at www.RnDSystems.com/go/Neuroscience

R&DSystems o�ers awide range of high quality products for neuroscience research. In addition to high performance

antibodies, we o�er the most referenced collection of premium quality proteins and ELISA kits in the industry.

Our catalog also includes primary rat andmouse cortical stem cells, and kits for the expansion, di�erentiation, and

identi�cation of neural stem cells.

Neuroscience ResearchR&D Systems Products for

R&D Systems Tools for Cell Biology Research™

Performance.

Results.

Progress.

Plexin-B2 Notch-2 O4

RGM-B HSPH1

GFAP BSRP-A Vanilloid R1