BD Falcon Cell Culture Multi-Flask · BD Falcon ™ Cell Culture Multi-Flask Multiply your cell...
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BD Falcon ™ Cell Culture Multi-Flask Multiply your cell growth. Multiply your productivity. Features • Even distribution of media across all layers for homogeneous cell growth. • Ability to mix cells and reagents in the BD Falcon Multi-Flask saves time and reduces risk of contamination. • Flexible design lets you pour or aspirate/recover cells using a pipet. • Consistent surface treatments for predictable scale-up. • Lot number is printed on each flask for traceability. • Manufactured in compliance to cGMP standards. Designed to Fit Your Protocol Simplify your scale-up. New BD Falcon Multi-Flasks offer the same footprint and the same reagent volumes and cell seeding densities per unit area as 175 cm² flasks. Plus, you’ll be using the same proven surface treatment as all other BD Falcon flasks. Improves Your Cell Culture Productivity BD Falcon Multi-Flasks deliver a thoughtful design that simplifies your workflow. You can eliminate multiple steps and reduce the risk of contamination. • Pipet access allows you to aspirate to replenish media and recover cells without pouring. • The mixing port enables rapid mixing inside the vessel and allows you to add your cell suspension, transfection, or other reagents directly to the flask. The port also enables uniform distribution of media and cells to facilitate homogeneous cell growth on all layers. More Consistent Cell Growth BD Falcon Multi-Flasks’ even distribution of media, proven vacuum- gas tissue culture surface treatment, and effective gas exchange all combine to provide an optimal cell culture environment. The result is high cell yield and a homogeneous cell population. Visit bdbiosciences.com/multiflask for more information. BD Falcon ™ Cell Culture Multi-Flasks enable you to grow more cells faster and easier, thereby making your cell culture workflow more productive. Tissue culture-treated BD Falcon Multi-Flasks are available in 3- or 5-layer formats and provide 525 cm 2 or 875 cm 2 cell growth surface area. They can be used with a wide range of liquid volumes (up to 50 ml/layer).
Transcript of BD Falcon Cell Culture Multi-Flask · BD Falcon ™ Cell Culture Multi-Flask Multiply your cell...
BD Falcon™ Cell Culture Multi-FlaskMultiply your cell growth. Multiply your productivity.
Features
• Evendistributionofmediaacrossalllayersforhomogeneouscellgrowth.
• AbilitytomixcellsandreagentsintheBDFalconMulti-Flasksavestimeandreducesriskofcontamination.
• Flexibledesignletsyoupouroraspirate/recovercellsusingapipet.
• Consistentsurfacetreatmentsforpredictablescale-up.
• Lotnumberisprintedoneachflaskfortraceability.
• ManufacturedincompliancetocGMPstandards.
Designed to Fit Your Protocol
Simplify your scale-up. New BD Falcon Multi-Flasks offer the same footprint and the same reagent volumes and cell seeding densities per unit area as 175 cm² flasks. Plus, you’ll be using the same proven surface treatment as all other BD Falcon flasks.
Improves Your Cell Culture Productivity
BD Falcon Multi-Flasks deliver a thoughtful design that simplifies your workflow. You can eliminate multiple steps and reduce the risk of contamination.
• Pipet access allows you to aspirate to replenish media and recover cells without pouring.
• The mixing port enables rapid mixing inside the vessel and allows you to add your cell suspension, transfection, or other reagents directly to the flask. The port also enables uniform distribution of media and cells to facilitate homogeneous cell growth on all layers.
More Consistent Cell Growth
BD Falcon Multi-Flasks’ even distribution of media, proven vacuum-gas tissue culture surface treatment, and effective gas exchange all combine to provide an optimal cell culture environment. The result is high cell yield and a homogeneous cell population.
Visit bdbiosciences.com/multiflask for more information.
BDFalcon™CellCultureMulti-Flasksenableyoutogrowmorecellsfasterandeasier,therebymakingyourcellcultureworkflowmoreproductive.
Tissueculture-treatedBDFalconMulti-Flasksareavailablein3-or5-layerformatsandprovide525cm2or875cm2cellgrowthsurfacearea.Theycanbeusedwithawiderangeofliquidvolumes(upto50ml/layer).
CONSISTENT CELL PHYSIOLOGY
CELL CULTURE VESSEL
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CHO-M1cellsweregrowninaT-175anda3-layerBDFalconMulti-Flaskfor72hours.Cellswereharvestedandre-seededon96-wellPDLplatesforGPCRCalciummobilizationassay.CellswerechallengedwithM1agonist,carbachol(300µM)andresponseswererecorded1minutefollowingcompoundaddition.Dataisexpressedasfold-increaseincalciumfluxcomparedtocontrolcellschallengedwithassaybuffer(nodrug).Nosignificantdifferenceinagonistresponsewasob-servedbetweencellsharvestedfromeithervesseltype.Datashownisrepresentativeofthreeindependentexperiments.
PREDICTABLE SCALE-UP
Expected Yield Actual Yield T-175 BD Falcon Multi-Flask (3-layer) BD Falcon Multi-Flask (5-layer)
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ThreeandfivetimesthenumberofBHK-21cellsweregrownandrecoveredfrom3-and5-layerBDFalconMulti-FlaskscomparedtoT-175flasks.Expectedyield(leftpanel)wasdeterminedusingmeancellyieldfromcontrolT-175flasksmultipliedbythreeandfivetimesforthe3-and5-layerBDFalconMulti-Flasks,respectively.Cellyieldpercm2(rightpanel)wasequivalentin3-and5-layerBDMulti-FlasksandT-175flasksforBHK-21,LnCap,Hep-G2andEcoPack™2-293cells.
SIMPLIFIED WORKFLOW
• Pipetaccesseliminatestheneedforpouring.Italsofacilitatestheuseofawiderangeofliquidvolumes(upto50ml/layer);efficientrecoveryofvaluablecellsandreagents;andminimizestheriskofcontamination.
• Mixingporteliminatesmixingoutsidethevessel,savingyoutimeandeffort.Thisuniquein-vesselmixingallowsrapidanduniformdistributionofcellsandreagentsinalllayersoftheflasks.
• CompatibilitywithsimilarvolumesofreagentusageandcellseedingdensitiesasT-175flasksmakesscalingupafamiliarandsimpleprocess.
CONSISTENT CELL GROWTH
IDEAL FOR MULTIPOTENT STEM CELLS
BD T-175 (control)Top Middle Bottom
ThisfigureillustratesuniformcellgrowthbetweenlayersofBDFalconMulti-Flasks.BHK-21cellsgrownto>80%confluencein3-layerBDFalconMulti-FlasksandcontrolT-175flaskswerefixedandstainedwithcrystalviolet.ControlflasksandindividuallayersoftheBDFalconMulti-Flaskswerecutandscanned.
HumanMesenchymalStemCells(hMSCs)wereculturedinMSCGM™medium(Lonza)andseededat4,000to5,000cells/cm2inT-175flasksand3-layerBDFalconMulti-Flasks.Cellswerecultureduntiltheyreached80%confluence(~6days).Cellswereharvested,counted,andcumulativepopulationdoublingsofhMSCsfromeachvesseltypewerecalculatedoverfiveconsecutivepassages.ImmunophenotypicpropertiesofhMSCsgrowninBDFalconMulti-Flasksasdeterminedbyflowcytometricanalysis(BDFACS™)confirmedthathMSCswerenegativeforsurfacemarkersCD14,CD34,CD45,CD79a,andHLA-DRandpositiveforexpressionofsurfacemarkersCD73,CD90,andCD105.Respectiveisotypecontrolsforeachmarkerantibodyareshowningrey.
COMPATIBLE WITH YOUR CELL LINES
BD Falcon T-175 Flask BD Falcon Multi-Flask (3-layer)
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Diversecelllinesandprimarycultures(withandwithoutserum)canbegrownandefficientlyrecoveredfromBDFalconMulti-Flaskcellculturevessels.ThisgraphillustratestheincreasedcellyieldonecanobtainfromBDFalconMulti-FlasksascomparedtocontrolT-175flasksforvariouscelllines.
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EcoPack is a trademark of Clontech Laboratories, Inc. MSCGM is a trademark of Lonza Cologne GmbH.BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2011 BDF11F268
BD Falcon Cell Culture Multi-FlaskMultiplyyourcellgrowth.Multiplyyourproductivity.
3-LAYER TISSUE CULTURE-TREATED, 525 CM2 2 12 353143
5-LAYER TISSUE CULTURE-TREATED, 875 CM2 1 8 353144
ORDER INFORMATIONBDFalconCellCultureMulti-Flask
ACCESSORIES
U.S. OrdersContact your authorized distributor to place your order.
Description Qty./Pack Qty./Case Cat. No.
Description Qty./Pack Qty./Case Cat. No.
BD Falcon Pipets
BD Falcon Tubes
BD Falcon Flasks
ASPIRATING PIPET 2 ML INDIVIDUALLY WRAPPED
50 ML POLYPROPYLENE CONICAL TUBE
To see the complete line of BD Falcon Flasks, visit www.bdbiosciences.com
175 ML POLYPROPYLENE CONICAL TUBE
225 ML POLYPROPYLENE CONICAL TUBE
ASPIRATING PIPET 5 ML INDIVIDUALLY WRAPPED
10 ML INDIVIDUALLY WRAPPED PAPER-PLASTIC
10 ML INDIVIDUALLY WRAPPED PLASTIC-PLASTIC
SEROLOGICAL PIPET 25 ML INDIVIDUALLY WRAPPED
SEROLOGICAL PIPET 100 ML INDIVIDUALLY WRAPPED
50 200 357558
25 500 352070
8 48 352076
8 48 352075
50 200 357501
50 200 357551
50 200 356551
50 200 356525
5 50 357600
BD BiosciencesTwo Oak ParkBedford, MA 01730tel: 877.232.8995fax: 800.325.9637bdbiosciences.com
U.S. Orderstel: 877.232.8995e-mail: [email protected]
Purchase Orders should be made out to:BD Biosciences2350 Qume DriveSan Jose, CA 95131
Outside the U.S.Contact your local distributor or visit bdbiosciences.com/offices to locate your nearest BD Biosciences office.
PRODUCT SPECIFICATIONS>5 ml per layer for dissociating
<50 ml per layer for cell expansion
0 to 50 ml per layer in 10 ml increments
10%
0.2 μm hydrophobic membrane
Tissue culture-treated, optically clear
WORKING VOLUME RANGE
MOLDED-IN GRADUATIONS
GRADUATION ACCURACY
CAP VENT MEMBRANE
CELL GROWTH SURFACE
