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BASF Nutrition –

the healthy decision

Natugrain ® TS -New and highly effective NSP-dehydrolyzing enzyme from BASF

Dr. habil. Ulrich EidelsburgerHead of Sales Nutrition Ingredients EuropeBASF ChemTrade GmbH, Burgbernheim, Germany

14th International Feed Technology Symposium

19-21 October 2010, Novi Sad, Serbia

from BASF

• Impact of NonStarchPolysaccharides (NSP) in animal nutrition

• Product characteristics

• Storage and processing properties

Outline

2BASF ChemTrade GmbH, Nutrition Ingredients Europe


• EU Approval and dosage recommendations

• Product performance in animal feeding

• Conclusions

Content of pentosans and β-glucans in cereals (g/kg)

Cereal Pentosans β-Glucans

total soluble total soluble

corn 40–70 5 1–2 –

wheat 50–80 11 5–9 –


rye 60–120 23 10–50 –

oats 40–80 8 20–50 16

barley 30–80 8 20–70 37

Jeroch, 1994 (modified)

NSP content in feed cerealsdependent on / influenced by:

• cultivar and type of the cereal

• climate during main growth and ripening phase (e.g. low temperature, wind, rain �� NSP content ↑)


• storage time after harvest (fresh cereals >> stored cereals)

• thermal processing in feed production(non-soluble NSPs partly transferred to soluble NSPs)

NSP

Digestaviscosity

Microflora

Cageeffect

← E ← EE →

Mode of action ofNon-Starch-Polysaccharides and NSP-dehydrolysing Enzymes


viscosity

Passagerate

Morphologic-structuralchanges

"Unstirredwater layer"

Nutrientabsorption

Protein andenergy

metabolism

flora effect

Fatdigestion

Overall effect: Feed intake, performance, nutrient conversion, ME, heat production

(Simon, 2005; modified)

Correlation between contents of NSP & AME value of cereals for broiler chicks


(Choct & Annison, 1990)




Outline





• Conclusions

Natugrain TSthe flexible NSP-enzyme


Natugrain TSpowder formulation

Natugrain TS Lliquid formulation

Enzyme activities:endo-1,4-ß-xylanase, min. 5600 TXU (Thermostable Xy lanase Unit) /gendo-1,4-ß-glucanase, min. 2500 TGU (Thermostable Gl ucanase Unit) /g




Outline





• Conclusions

Pelleting stabilityat 3 different temperatures

60

80

100

Res

idua

l enz

yme

activ

ity [%

] • excellent pelleting stabilityup to 85°C*


0

20

40

60

75 80 85

°C

Endoxylanase (TXU) Betaglucanase (TGU)

Res

idua

l enz

yme

activ

ity [%

]

*conditiong time used in the trial: 20 s

Pelleting stabilityComparison with competitor productsPelleting temperature 85 °C

80

90

100

Res

idua

l enz

yme

activ

ity [%

] Natugrain TS (Product A)• best pelleting stability at 85°Ccompared to competitors

Competitor Products B, C and D

11BASF ChemTrade GmbH, Nutrition Ingredients EuropeLiHo-04-08

40

50

60

70

Glucanase*

Xylanase*

A B C D

*analysed with BASF method for TGU and TXU

Res

idua

l enz

yme

activ

ity [%

]

Competitor Products B, C and D tested at recommended dosages

60

80

100

Stability in pelleted feedStored at 35 °C

Res

idua

l enz

yme

activ

ity [%

] • excellent stability in pellets stored at high temperature

Natugrain TS


0

20

40

60

0 6 10

weeks


Res

idua

l enz

yme

activ

ity [%

]

BASF 2007BASF 2007

60

80

100

Stability in premixStored at 35 °C

Res

idua

l enz

yme

activ

ity [%

] • excellent stability in premix stored at high temperature

Natugrain TS


0

20

40

60

0 6 12 18

weeks


Res

idua

l enz

yme

activ

ity [%

]

BASF 2007

Shelflife on pellets after PPLAStored at 35 °C

Natugrain TS L (liquid)

Res

idua

l enz

yme

activ

ity [%

] • excellent stability on pellets stored at high temperature

60

80

100


Res

idua

l enz

yme

activ

ity [%

]

0

20

40

60

0 6 10 16

weeks

Endoxylanase (TXU) Betaglucanase (TGU)BASF 2007




Outline





• Conclusions

EU Approval of Natugrain TS and recommended dose rates

approved min. dose rate recommended dose rate

� Broiler 50 g/t

� Duck 50 g/t

Wheat, barley and/or

up to 40 % 50 g/t

40 – 50 % 75 g/t


� Duck 50 g/t

� Laying hen 100 g/t 100 g/t

� Turkey 100 g/t 100 g/t

� Piglets (weaned) 100 g/t 100 g/t

� Fattening pigs 100 g/t (expected Q3 2011)

and/or rye over 50 % 100 g/t

• Impact of NonStarchPolysaccarids (NSP) in animal nu trition



Outline





• Conclusions

Improvements in piglet performance Trial period: 0- 42 days after weaning

Trial Parameter Control Natugrain % improvement

Italy, 2006 DWG (g) 335 384 14.6

FCR (g/g) 1.58 1.44 8.8


Hungary, 2007a DWG (g) 447 474 6.0

FCR (g/g) 1.97 1.88 4.6

Hungary, 2007b DWG (g) 475 498 4.8

FCR (g/g) 1.8 1.76 2.2

Influence of NSP enzymes on feces quality in weaned piglets fed wheat and barley based diets

% days of the experimental period with soft / aqueous feces in the piglet trial

12,8

12

14


6,9

8,9

0

2

4

6

8

10

12

Control + Natugrain TS + Competitor B

%

Source: BNA, 2009

Laying performance from week 26 to 78(Natugrain TS 100 g/t; 50% wheat, 10% rye)

Control Natugrain TS

26 – 52week

52 – 78week

Overall26 – 78week

26 – 52week

52 – 78week


Laying rate, % 93.6 87,0 90.4 93.2 87.2 90.3


Daily egg mass, g 57.6 57,0 57.3 57.4 57.3 57.4

Egg weight, g 61.5 65.5 63.4 61.6 65.7 63.6

Daily feed intake, g 119.2a 125.9a 122.5a 114.8b 119.0b 116.9b

Feed conversion, g/g 2.070a 2,212a 2.138a 1,999b 2,078b 2.037b

Dirty eggs, % 3.7a 4.4 4.0a 3.0b 4.0 3.4b

(Van der Klis and Lensing, 2007)

Effect on nutrient digestibility and energy content **(Natugrain TS 100 g/t; 50% wheat, 10% rye)

Nutrient Control Natugrain TS

Organic matter, % 73.4 b 74.9 a

Fat, % 69.6 74.4*


Protein, % 79.4 b 80.4 a

Carbohydrates, % 71.9 b 73.2 a

AME N, kcal/kg DM 2868 a 2951 b

*P = 0.064

** digestibility study with 25 weeks old Dekalb hens(Van der Klis and Lensing, 2007)

Control Natugrain TS

26 – 52week

52 – 78week


26 – 52week

52 – 78week


Laying rate, % 93.6 87,0 90.4 93.2 87.2 90.3

Laying performance from week 26 to 78(Natugrain TS 100 g/t; 50% wheat, 10% rye)


Daily egg mass, g 57.6 57,0 57.3 57.4 57.3 57.4

Egg weight, g 61.5 65.5 63.4 61.6 65.7 63.6

Daily feed intake, g 119.2a 125.9a 122.5a 114.8b 119.0b 116.9b

Feed conversion, g/g 2.070a 2,212a 2.138a 1,999b 2,078b 2.037b

Dirty eggs, % 3.7a 4.4 4.0a 3.0b 4.0 3.4b


2,182,13

2,2

2,3

Effect on water: feed ratio(Natugrain TS 100 g/t; 50% wheat, 10% rye)


2,052,09

2,02 2,04

1,9

2

2,1

26-52 52-78 26-78

week

Control Natugrain TS 100 g/t

[g /

g]


Day 0 – 105 (15 weeks)

2,352,35

2,40

Turkey feeding trial Effect on Feed Conversion Rate(Wheat & barley based diet, + Natuphos 5000 G (EU) 100 g/t)


Source: BNA 2009

2,242,25

2,26

2,20

2,25

2,30

per bi

rd [g

/g]

Control Natugrain® TS, 100 g/tCompetitor A Competitor B

Day 0 – 105 (15 weeks)

64000

65000

66000b

(P < 0.001)

Turkey feeding trial Effect on Water Intake(Wheat & barley based diet, + Natuphos 5000 G (EU) 100 g/t)


58000

59000

60000

61000

62000

63000

per bi

rd [g

]


aa

a

Source: BNA 2009

Day 0 – 105 (15 weeks)

2,15

2,20

Turkey feeding trial Effect on Water / Feed Ratio(Wheat & barley based diet, + Natuphos 5000 G (EU) 100 g/t)


2,00

2,05

2,10

per bi

rd [g

]


Source: BNA 2009

Day 99

42,00

43,00

44,00 (P < 0.01)c

bc

Turkey feeding trial Effect on Litter Dry Matter(Wheat & barley based diet, + Natuphos 5000 G (EU) 100 g/t)

%


37,00

38,00

39,00

40,00

41,00

42,00


a

ab�� positive influence

on hygienic condtions

Source: BNA 2009

Improvement in broiler performance (Wheat based diets)

Trial Period (d) Parameter Control Natugrain(100 g/t)

% improvement

Poznan, 2007 0-42 LW (g) 1959a 2429b 24.0

FCR (g/g) 2.08b 1.82a 12.5


Feedtest, 2007 0-35 LW (g) 1866a 2298b 23.2

FCR (g/g) 2.20b 1.80a 18.0

BASF, 2007 a 0-35 LW (g) 1976a 2136b 8.1

FCR (g/g) 1.94b 1.60a 17.5

BASF, 2007 b 0-35 LW (g) 1866a 2298b 23.2

FCR (g/g) 2.20b 1.80a 18.2

1968 19841943

1907

1850

1950

2050

controlNatugrain TS (50g/t)

bb

b

b

(P < 0.05)

Broiler feeding trial Effect on Final body weightWheat 50-55%, barley 10-12%


1641

1450

1550

1650

1750Natugrain TS (50g/t)Natugrain TS (100g/t)Competitor ACompetitor B

a[g]

�� All products tested improved final body weight sign ificantly versus controlNatugrain TS showed best performance even at a dosa ge of 50 g/t

Source: BASF 2008

2,121

1,847 1,831 1,85

1,908

1,9

2

2,1

2,2

controlNatugrain TS (50g/t)

a

b bb

b

[g /

g]

(P < 0.05)

Broiler feeding trial Effect on Feed Conversion RateWheat 50-55%, barley 10-12%


1,847 1,831

1,5

1,6

1,7

1,8

1,9 Natugrain TS (50g/t)Natugrain TS (100g/t)Competitor ACompetitor B

[g /

g]

Source: BASF 2008

�� All products tested improved feed conversion rate s ignificantly vs controlNatugrain TS showed best performance even at a dosa ge of 50 g/t

Litter ScoreBroiler trial (Ross: day 28)

Diet Average

Wheat 60.0

Corn 8.0

Soy 25.1

Soy oil 0.2

Palm oil 3.0

Group EnzymeDose (g/T)

1 Negative Control -

2 Competitor A 50

3 Competitor B 100

4 Competitor C 1504.50

31BASF ChemTrade GmbH, Nutrition Ingredients EuropeLiHo-70-04

categories: 4 = normal3 = less dirty2 = dirty1 = very dirty

Source: BNA, 2006

5 Competitor D 20

6 Natugrain Wheat TS 50

7 Natugrain Wheat TS 100

8 Natugrain TS 1003.08

3.25 3.29

3.50

3.13

3.42

3.54 3.54

2.00

2.50

3.00

3.50

4.00

4.50

T- Rov. Belf. Ron. Saf. N1 N2 NG11 2 3 5 64 7 8




Outline





• Conclusions

Conclusions

� Approvals for major animal species allow multiple and flexible use

� Significantly improved animal performance due to high efficacy in cereal based diets

� Pronounced positive effects on faeces and litter quality


� Pronounced positive effects on faeces and litter quality

� Adds flexibility for diet composition

� Exceptional stability and excellent processing properties ensure a reliable and consistent performance

TAKE THE CHANCE. PICK THE ECONOMICAL SOLUTION !

Natugrain TS – the highly effective and flexible NSP -Enzyme


Thank you very much for your attention

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