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Pengukuran KadarMDA (Lailatul et al,2015)

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Lipid peroxidation (LPO)

LPO levels were estimated with thio-barbituric acid (TBA!) a"d color reactio" #or mala"dialdeh$de

(%&A)

accordi"' to rocedure b$ Placer et al, (1*++)10 Tissues were homo'e"i.ed i" chilled 015 % /l

usi"' a

Teo" estle to obtai" 10 w3v homo'e"ate O"e ml o# homo'e"ate was i"cubated at 46 (7 05) #or

two hoursTo each samle, 1 ml o# 10 w3v tricholoro acetic acid (TA) (s d 8"e chem Ltd9 %umbai) was added

A#ter

throu'h mi:i"', the reactio" mi:ture was ce"tri#u'ed at 2000 rm #or 10 mi"utes 1 ml o# suer"ata"t

was ta;e" with

e

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camura" diiet da" dimasu;;a" ;e dalam tabu"' rea;si Tabu"' rea;si ditambah

1 mL rea'e" Moli"-iocalteu da" 2 mL 20 Ca2O4, lalu dibiar;a" berdiri te'a;

selama 4 me"it !etelah itu laruta" dalam tabu"' rea;si dii";ubasi ada rua"'

'ela da" suhu rua"'a" selama D5 me"it, lalu dise"tri#us selama 5 me"it ada DDJ

g !uer"ata" diambil da" se'era dibaca seraa""$a ada N 40 "m !eba'ai

sta"dar di'u"a;a" oro'lusi"ol /adar orota"i" di"$ata;a" de"'a" setara m'oro'lusi"ol tia ' e;stra;

Superoksida Dismutase (Misra and Fridovich, 192)

Pri"si metode i"i berdasar;a" ;emamua" !O& dalam me"'hambat autoo;sidasi

ei"e#ri" me"adi ade"o;rom !atu u"it a;tivitas !O& adalah ;emamua"

me"'hambat 50 autoo;sidasi ei"e#ri" me"adi ade"o;rom ar"a co;lat dari

ade"o;rom diu;ur secara se;tro#otometer ada a"a"' 'elomba"' DJ0 "m

Katalase (Sinha, 192)

Pri"si metode $a"' di;emba"';a" oleh !i"ha me"''u"a;a" .at war"a seba'ai

i"di;ator at war"a $a"' di'u"a;a" adalah otasium bi;romat (/2r2O ) 5

dalam suasa"a asam asetat 'lasial (1K4) Qo" bi;romat dalam suasa"a asam a;a"

diredu;si oleh 2O2 me"adi ;romat da" memberi;a" war"a ada a"a"'

'elomba"' 50 "m 1 u"it a;tivitas ;atalase di"$ata;a" seba'ai ba"$a;"$a 2O2

dalam mol $a"' di'u"a;a" oleh ;atalase tia me"it

Laruta" ui da" sta"dar seba"$a; 1 mL diiet da" dimasu;;a" ;e dalam tabu"'rea;si !ela"ut"$a ditambah 2 mL i"di;ator ewar"a Tabu"' rea;si dia"as;a"

ada air me"didih selama 10 me"it !etelah di"'i", baca seraa""$a ada a"a"'

'elomba"' 50 "m !eraa" $a"' terbaca setara de"'a" ;o"se"trasi

!lutation "eroksidase ("a#lia and $alentine, 19%)

Pri"si metode i"i berdasar;a" ;emamua" 'lutatio" ero;sidase me"';atalisasi

'lutatio" teredu;si me"adi 'lutatio" tero;sidasi lutatio" tero;sidasi diredu;si

;embali me"adi 'lutatio" teredu;si oleh e".im 'lutatio" redu;tase de"'a" ;o#a;tor

CA&P dalam suasa"a asam Rumlah 'lutatio" teredu;si diu;ur de"'a" me"e"tu;a"

umlah mi;romol CA&P seba'ai eredu;si $a"' diu;ur secara se;tro#otometer

ada a"a"' 'elomba"' 4D0 "m

Prosedur a"alisis Bu#er #os#at 0,1 % ,0 seba"$a; 200 @L, 200 @L samel, 200

@L 'lutatio" 10 m%, da" 200 @L 'lutatio" redu;tase 2,D ?"it dicamur amura"

laruta" tersebut sela"ut"$a dii";ubasi selama 10 me"it ada suhu 4o

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!ela"ut"$a ;e dalam laruta" ditambah;a" 200 @L CA&P 1,5 m% da" dii";ubasi

la'i ada suhu $a"' sama selama 4 me"it A;hir"$a 2O 2: +,22 Fs

serapan x Vt 1,5 mM sebanyak 20 L ditambahkan dalam campuran tersebut. Serapan larutan dibaca di

antara aktu 1!2 menit pada pan"an# #el$mban# %&0 nm.

-%ai;el osabal a, Mabie" Pierro" b , c, Patrice outure a, %a'alie Baudrimo"t b , c,

La"dis are a, Peter ambell a 2015 !ubcellular artitio"i"' o# "o"-

esse"tial trace metals (A', As, d, Ci,

Pb, a"d Tl) i" livers o# America" (A"'uilla rostrata) a"d Iuroea"

(A"'uilla a"'uilla) $ellow eelsA