Bachelor's Defense

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Department of NEXS Copenhagen, 24th of June, 2013 Rune Rasmussen Department of Physical activity and the Brain Bachelor defense Spontaneous locomotion in young NMRI mice following motherly LPS treatment

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This presentation was for the defense of my Bachelor's degree thesis at the University of Copenhagen.

Transcript of Bachelor's Defense

  • 1.Department of NEXS Copenhagen, 24th of June, 2013 Rune Rasmussen Department of Physical activity and the Brain Bachelor defense Spontaneous locomotion in young NMRI mice following motherly LPS treatment

2. Department of NEXS Background Copenhagen, 24th of June, 2013 (Boksa, 2010) 3. Department of NEXS Background Copenhagen, 24th of June, 2013 Spontaneous locomotion ? 4. Department of NEXS Aim of study Induces prenatal infection changes in the level of spontaneous locomotion ? Do the level of spontaneous locomotion differ between genders in NMRI mice? Copenhagen, 24th of June, 2013 5. Department of NEXS Aim of study Induces prenatal infection changes in the level of spontaneous locomotion ? Do the level of spontaneous locomotion differ between genders in NMRI mice? Copenhagen, 24th of June, 2013 6. Department of NEXS Groups Copenhagen, 24th of June, 2013 n = 7 n = 8 n = 8 Con-male Con-female $$ $$ 7. Department of NEXS Protocol Copenhagen, 24th of June, 2013 PREopen-eld PREopen-eld POST open-eld POST open-eld 8. Department of NEXS Open-field tests Copenhagen, 24th of June, 2013 LPS mice travelled a shorter distance in open-field test Con-male only different in POST test * different from PRE different from LPS (p No difference 12 weeks after treatment *Immunohistochemistry conducted by Hanne Mikkelsen, ICMMCopenhagen, 24th of June, 2013 Inflammatory response disappears quickly after ended treatment (Stolp et al. 2005) LPS Con 13. Department of NEXS Future work in astrocytes (Weekendavisen, 14th of June, 2013) (Maiken Nedergaard, M.D, D.M.Sc) Copenhagen, 24th of June, 2013 14. Department of NEXS (Stolp et al. 2005) Copenhagen, 24th of June, 2013 Increased astrocyte density Astrocytes 15. Department of NEXS Impaired neuron-glia interaction? in vivo imaging of astrocyte- and neuronal activity (Perea et al. Trends in neuroscience, 2009) (SmithLab, University of Stanford, 2013) Copenhagen, 24th of June, 2013 16. Department of NEXS The corticospinal tract LPS treatment GD 11-13 vs. 18-20? (Wikipdia, 2013)Copenhagen, 24th of June, 2013 Last round of DNA synthesis of layer V precursor cells GD 15-18 (Standford et al. 1992) 17. Wrong interpretation of POWER Statistical power is the probability for not committing a type II error (False negative) Significance level is the probability for committing a type I error (False positive) => Makes no sense to apply the power-test on significant results! Department of NEXS Copenhagen, 24th of June, 2013 18. Department of NEXS Rune Rasmussen Department of Nutrition, Exercise and Sports Copenhagen, 24th of June, 2013 Bachelor defence 19. Enhedens navn Copenhagen, 24th of June, 2013 How does the LPS work? 20. Department of NEXS Peak speed 3 groups Copenhagen, 24th of June, 2013 21. Enhedens navn Inner zone entrances 3 groups Copenhagen, 24th of June, 2013 22. Department of NEXS Copenhagen, 24th of June, 2013 Rearing's 3 groups 23. Department of NEXS Copenhagen, 24th of June, 2013 Distance 6 groups 24. Enhedens navn Peak speed 6 groups Copenhagen, 24th of June, 2013 25. Enhedens navn Inner zone entrances 6 groups Copenhagen, 24th of June, 2013 26. Enhedens navn Rearing's 6 groups Copenhagen, 24th of June, 2013 27. Department of NEXS Copenhagen, 24th of June, 2013 Voluntary wheel-running 12 days 28. Department of NEXS Weight treated mice Copenhagen, 24th of June, 2013 29. Enhedens navn Weight offspring Copenhagen, 24th of June, 2013