B ZERO AFLA M1 - Easy and cost effective ELISA test kit for aflatoxin M1 screening in milk
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Transcript of B ZERO AFLA M1 - Easy and cost effective ELISA test kit for aflatoxin M1 screening in milk
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B ZERO AFLA M1
The most rapid and cost effective ELISA test kit for aflatoxin M1
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Aflatoxin M1
• Aflatoxin M1 is a toxic metabolite resulting from the
digestion of Aspergillus Flavus mycotoxin aflatoxin B1
in dairy animals; the main source of contamination are feed and cereals.
• Aflatoxin M1 is particularly toxic for liver and kidneys.
• Aflatoxin M1 is excreted in milk within 24 hours from
intake of contaminated feed. Clearance is reached within 1 and 4 days from contamination.
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Product overview
• Assay principle: competitive enzyme immunoassay
• Application: raw milk
• Measuring range: 10 – 200 ng/l
• Detection limit: 10 ng/l aflatoxin M1 in milk
• Assay time: 30 minutes
• Sample preparation: none
• Limit of quantification: 20 ng/l(100% sensitivity)
• Mean intra-assay CV: 5%
• Mean inter-assay CV: 8%
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The B ZERO format
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The B ZERO format
• The use of just one standard and the absence of any replicate make B ZERO AFLA M1 the most cost effective solution for aflatoxin M1 screening.
• B ZERO brings together speed, ease of use, and reliability in a single ELISA kit.
Ideal for who implements few
samples per analysis as well as who has
high throughput needs!
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About sample preparation
• Sample preparation is a fundamental step for any analysis; its complexity has a relevant incidence on the total time the analysis requires.
• For aflatoxin M1 analysis, traditional ELISA kits usually require to cool
the milk sample, to centrifuge it for approximately 10 minutes and to take away the fat layer.
Bottle-neck procedure when many samples have to be analysed
Additional source of error
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How many samples can you centrifuge together?
How many vials do you need to defat your samples?
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How much time do you need to prepare your milk samples?
![Page 9: B ZERO AFLA M1 - Easy and cost effective ELISA test kit for aflatoxin M1 screening in milk](https://reader037.fdocuments.us/reader037/viewer/2022102723/55892b19d8b42aae088b4640/html5/thumbnails/9.jpg)
Avoidingmilk manipulation
• TECNA B ZERO AFLA M1 is
the first ELISA kit for
aflatoxin M1 that brings
together the high quality
performances of
traditional ELISA tests and
strip-tests ease of use.
Thanks to the highly specific antibody and to the perfect in-matrix calibration B ZERO AFLA
M1 is the first ELISA kit
that does not require defatting to analyze
aflatoxin M1 in milk!
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• Specific experiments were performed in order to evaluate the correlation
between results of analysis of defatted and not defatted milk on more than 60 samples
• No accuracy or precision statistical differences aroused when analyzing not defatted milk (correlation between defatted and not defatted milk was r2=0.96)
ng/L defatted milk samples
ng/L
not
def
atted
Performance assessment
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Why B ZERO AFLA M1?
NO milk manipulation- Save on time and lab equipment- Reduce any source of error
Results in just 30 MINUTESNO calibration curve needed- Save on wells dedicated to calibrators
No need to run any replicate
Visit us at www.tecnalab.com!