Axon Guidance Using Microfluidic Devices
description
Transcript of Axon Guidance Using Microfluidic Devices
Axon Guidance Using Microfluidic DevicesMadelyn S. Luttgen Mentor: Dr. Noo Li Jeon
http://nljgroup.eng.uci.edu/, www.calit2.net
10 nM Nogo
100 nM Nogo
Control
Map 5 Fluorescence
Gradient device will allow for easier
detection of Nogo activity and new
experiments concerning axon
guidance
In the CNS, axons do not regenerate after injury
Nature Reviews Drug Discovery 2; 872-879 (2003); doi:10.1038/nrd1228
Myelin proteins MAG,
Omgp, and Nogo bind to
the same receptor to inhibit axon
regeneration
CNTL 1 10 100 500 10000
20
40
60
80
100
Ax
on
le
ng
th (
% o
f C
on
tro
l)
Concentration of NOGO (nM)
Data Summary
Fabricated devices have a 150 micrometer ladder like groove region surrounded by two 1.5
millimeter channels. The cells are loaded into one side and the axons will extend through the
microgroove region into the other channel.
This research examines the activity of the Nogo protein. To replicate central nervous system injury, different concentrations of Nogo are
added to individual devices after the axons are cut, and single axon regrowth can be directly
measured.