ASMS 2011 Using Thermal Gradient Focusing ESI to Develop · ASMS 2011 ThP 475 Using Thermal...
4
ASMS 2011 ThP 475 Using Thermal Gradient Focusing ESI to Develop Sensitive, High-Throughput Capillary Flow LC/MS/MS Peptide Quantitation Assays Craig Love, Christine A. Miller, and Alex Mordehai Agilent Technologies, Santa Clara, CA, USA
Transcript of ASMS 2011 Using Thermal Gradient Focusing ESI to Develop · ASMS 2011 ThP 475 Using Thermal...
ASMS 2011
ThP 475
Using Thermal Gradient
Focusing ESI to Develop
Sensitive, High-Throughput
Capillary Flow LC/MS/MS
Peptide Quantitation
Assays
Craig Love, Christine A. Miller, and Alex Mordehai Agilent Technologies, Santa Clara, CA, USA
ASMS 2011 Page 2
Using Thermal Gradient Focusing ESI to Develop Sensitive, High-Throughput Capillary Flow LC/MS/MS
Peptide Quantitation Assays
ASMS 2011 Page 3
Using Thermal Gradient Focusing ESI to Develop Sensitive, High-Throughput Capillary Flow LC/MS/MS
Peptide Quantitation Assays
ASMS 2011 Page 4
Using Thermal Gradient Focusing ESI to Develop Sensitive, High-Throughput Capillary Flow LC/MS/MS
Peptide Quantitation Assays
![StructuralBasisfortheEnzymaticFormationof ...NH 4)HCO 3 gradient(0–0.4M)in300ml.Fractionscontaining [4R-2H]NADHwerepooledandlyophilized.Purityanddegree ofdeuterationweredeterminedbyLC-UV/ESI-MSn](https://static.fdocuments.us/doc/165x107/5b077b1d7f8b9ad5548e6ce5/structuralbasisfortheenzymaticformationof-nh-4hco-3-gradient004m-4r-2hnadhwerepooledandlyophilizedpurityanddegree.jpg)
