ASEA Metabolomics Results - Amazing Moleculesamazingmolecules.com/pdf/ASEA Metabolomics...

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ASEA Metabolomics Results Nieman DC. Human Performance Laboratory, North Carolina Research Campus and Appalachian State University

Transcript of ASEA Metabolomics Results - Amazing Moleculesamazingmolecules.com/pdf/ASEA Metabolomics...

Page 1: ASEA Metabolomics Results - Amazing Moleculesamazingmolecules.com/pdf/ASEA Metabolomics Results.pdf · ASEA Metabolomics Results Nieman DC. Human Performance Laboratory, North Carolina

ASEA Metabolomics Results Nieman DC. Human Performance Laboratory, North Carolina Research Campus and

Appalachian State University

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Metabolomics Laboratory, North Carolina Research Campus,

David H. Murdock Research Institute

Slide 1 Appalachian state

Human Performance Laboratory

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• 75-km cycling

1-week ASEA

• 75-km cycling

1 week Placebo

Crossover BASELINE

TESTING for VO2max,

body composition

(N=20 subjects)

Blood/urine : Pre-Ex Post- Ex 1-h

• 75-km cycling

1-week ASEA

• 75-km cycling

1-week Placebo

Blood/urine : Pre-Ex Post- Ex 1-h

3-w

eek

was

hout

Metabolomics: Goal is to measure the influence of ASEA on small molecules (metabolites) that shift in response to

supplementation. The shift in metabolites, depending on the

nutritional product, may represent effects on inflammation, oxidative

stress, and physiologic stress.

Slide 2 Appalachian state

Human Performance Laboratory

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Working Summary

• Seven days ingestion of ASEA (relative to placebo) caused an extensive mobilization of free fatty acids from adipose tissue in male cyclists.

• Athletes on ASEA for 7-days started the 75-km cycling trial with high blood free fatty acids leading to increased fat oxidation and a sparing of amino acids (and potentially muscle glycogen).

• ASEA intake was associated with a large increase in serum ascorbic acid levels (probably from the adrenal cortex).

• Serum creatinine and urea also increased post-exercise.

Slide 3 Appalachian state

Human Performance Laboratory

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2) Acute Effect: Increased fatty acid oxidation and mobilization during exercise (placebo condition was linked to a late mobilization).

Finding 1: Ingestion of ASEA beverage for one week strongly increased serum fatty acids levels (most likely from adipose tissue). 1) Chronic Effect: Higher fatty acid levels pre-exercise (several types of fatty acids --- see slides).

Triglyceride Mobilization: corresponding with the increase in free fatty acids, glycerol was higher at baseline (indicative of extensive adipose triglyceride hydrolysis).

FFAs Glycerol

TG Hydrolysis

+ with ASEA

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Myristic acid 14C Saturated Fatty Acid

FDR=6.49E-32

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Myristic acid 14C Saturated Fatty Acid

FDR=2.61E-20

ASEA Placebo

Slide 4 Appalachian state

Human Performance Laboratory

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Post 7-day Ingestion: Fatty Acids Higher in ASEA vs. Placebo

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Myristic acid 14C Saturated Fatty Acid

FDR=6.49E-32

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Palmitic Acid 16C Saturated Fatty Acid

FDR=1.86E-25

02000400060008000

1000012000140001600018000

ASEA PlaceboLeas

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Oleic Acid 18C Monounsaturated n9 Fatty Acid

FDR=5.21E-18

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Stearic Acid 18C Saturated Fatty Acid

FDR=3.22E-12

Slide 5 Appalachian state

Human Performance Laboratory

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020406080

100120140160

ASEA Placebo

Capric Acid 10C Saturated Fatty Acid

FDR=5.39E-07

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Glycerol Backbone of Triglycerides

FDR=9.49E-07

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Palmitelaidic Acid 16C Trans Fatty Acid

FDR=1.13E-08

7-days Ingestion of ASEA Fatty Acids and Glycerol Backbone: Higher in ASEA vs. Placebo

Slide 6 Appalachian state

Human Performance Laboratory

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Oleic Acid 18C Monunsaturated n9 Fatty Acid

FDR = 7.96E-10

ASEA Placebo

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Palmitelaidic Acid 16C Trans Fatty Acid

FDR=1.66E-16

ASEA Placebo

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Palmitic Acid 16C Saturated Fatty Acid

FDR=1.56E-20

ASEA Placebo

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Myristic acid 14C Saturated Fatty Acid

FDR=2.61E-20

ASEA Placebo

Serum Fatty Acids During Exercise

Slide 7 Appalachian state

Human Performance Laboratory

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Capric Acid 10C Saturated Fatty Acids

FDR=0.0059

ASEA Placebo

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Lauric Acid 12C Saturated Fatty Acids

FDR=0.0281

ASEA Placebo

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Stearic Acid 18C Saturated Fatty Acids

FDR=1.33E-06

ASEA Placebo

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Glyercol Monosterate FDR = 0.0060

ASEA Placebo

Serum Fatty Acids During Exercise

Slide 8 Appalachian state

Human Performance Laboratory

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Acetyl Co-A

Citrate

Isocitrate

alpha- Ketoglutarate

Succinyl CoA Succinate

Fumarate

Malate

Oxaloacetate

Pyruvate

Pre Post 1H Post

Serum Leucine FDR=0.0004

Pre Post 1H Post

Serum Aspartate FDR=0.0075

Pre Post 1H Post

Serum Fumarate FDR=1.06E-06

In urea cycle

Via Beta Oxidation

Via Glutamate

Pre Post 1H Post

Serum Valine FDR=0.0089

Via Odd Chain Beta Oxidation

Pre Post 1H Post

Serum Proline FDR=0.0066

Pre Post 1H Post

Serum Threonine FDR=0.0108

Graph Key

ASEA Placebo

Pre Post 1H Post

Serum Glycine FDR=0.0162

Pre Post 1H Post

Serum Serine FDR=0.0273

Pre Post 1H Post

Serum Malate FDR= 0.0004

Pre Post 1H Post

Serum Citrate FDR = 0.0037

Finding 2: High levels of blood free fatty acids were linked to a sparing of amino acid catabolism, and increased Krebs Cycle intermediates, post-exercise

Slide 9 Appalachian state

Human Performance Laboratory

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Leucine Krebs Entry: alpha Ketoglutarate

FDR =0.0004

ASEA Placebo

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Proline Krebs Entry: alpha Ketoglutarate

FDR = 0.0066

ASEA Placebo

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Aspartate Krebs Entry: Oxaloacetate

FDR = 0.0074

ASEA Placebo

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Valine Krebs Entry: Succinyl CoA

FDR = 0.0089

ASEA Placebo

Serum Amino Acids at Pre, Post, and 1H Post-Exercise “Sparing” of Amino Acids with ASEA

Slide 10 Appalachian state

Human Performance Laboratory

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Serum Amino Acids at Pre, Post, and 1H Post-Exericse “Sparing” of Amino Acids with ASEA

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Threonine Krebs Entry: Pyruvate

FDR= 0.0108

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Serine Krebs Entry: Pyruvate

FDR= 0.0273

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Glycine Krebs Entry: Pyruvate

FDR= 0.0162

Graph Key

ASEA Placebo

Slide 11 Appalachian state

Human Performance Laboratory

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Serum Krebs Intermediate at Pre, Post, and 1H Post-Exercise Higher Levels with ASEA

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Fumarate FDR = 1.06E-06

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Malate FDR= 0.0004

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Citrate FDR = 0.0037

Graph Key

ASEA Placebo

Slide 12 Appalachian state

Human Performance Laboratory

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3) Ascorbic Acid Metabolism: ASEA supplementation appears to be affecting ascorbic acid both acutely and chronically.

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Fructose FDR=1.12E-05

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Threonic Acid FDR=1.46E-98

Chronic Differences: ASEA group has lower baseline levels of fructose and lower levels of threonic acid. Fructose is broken down into ascorbic acid which is further metabolized into threonic acid. This could be suggestive of higher ascorbic acid production but no differences in groups were detected at baseline.

Acute Differences: ASEA group has higher levels of ascorbic acid, an antioxidant, and lower levels of both fructose and threonic acid.

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Ascorbic Acid FDR = 5.6E-06

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Fructose FDR = 0.0002

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Threonic Acid FDR=0.0031

Graph Key

ASEA Placebo Slide 13 Appalachian state

Human Performance Laboratory

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Serum Creatinine FDR=2.55 E-06

Graph Key

ASEA Placebo

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Aminomalonic Acid FDR = 1.13E-05

Breakdown product of creatine Plays role in binding calcium to protein

4) Other Changes. Some other changes were found both acutely and chronically that require further investigation into implications.

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Urea FDR = 0.0108

Formed in liver; Removal of nitrogen and ammonia

Slide 14 Appalachian state

Human Performance Laboratory

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Blood Urinary Nitrogen (BUN) Normal Range: 8-20 mg/dl

PlaceboASEA

BUN levels did not differ between treatment (treatment x time p-value=0.9743)

Slide 15 Appalachian state

Human Performance Laboratory

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00.20.40.60.8

11.21.4

Pre Post 1H

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Creatinine Normal Range: 0.7-1.2 mg/dl

PlaceboASEA

Creatinine levels did not differ between treatment (treatment x time p-value=0.7717)

Slide 16 Appalachian state

Human Performance Laboratory

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Bilirubin Normal Range: 0.3-1.2 mg/dl

PlaceboASEA

Bilirubin levels did not differ between treatment (treatment x time p-value=0.9971)

Slide 17 Appalachian state

Human Performance Laboratory

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Alkaline Phosphatase Normal Range: 39-117 IU/L

PlaceboASEA

Alkaline Phosphatase levels did not differ between treatment (treatment x time p-value=0.8819)

Slide 18 Appalachian state

Human Performance Laboratory

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AST Normal Range: 15-41 IU/L

PlaceboASEA

AST levels did not differ between treatment (treatment x time p-value=0.9546)

Slide 19 Appalachian state

Human Performance Laboratory

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IU/L

ALT Normal Range: 17-63 IU/L

PlaceboASEA

ALT levels did not differ between treatment (treatment x time p-value=0.9739)

Slide 20 Appalachian state

Human Performance Laboratory