Ascomycota (continued) MIKROSKOPIK
description
Transcript of Ascomycota (continued) MIKROSKOPIK
![Page 1: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/1.jpg)
Ascomycota (continued)
MIKROSKOPIK
YEAST, RAGI, KHAMIR… DLL
KULIAH 7
![Page 2: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/2.jpg)
PEMBAGIAN ASCOMYCOTA
Class 1: Laboulbeniomycetesparasitic attachments to arthropods.
Class 2: Protoascomyceteslack of ascogenous hyphae and ascomata.
Class 3: Euascomycetesmost of the fungi which form ascomata. The orders are separated on the structure of the ascus and the manner of ascus opening.
![Page 3: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/3.jpg)
From Spatafora et al. 2006. A five-gene phylogeny of Pezizomycotina. Mycologia 98: 1018-1028
![Page 4: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/4.jpg)
Filamentous ascomycetes
Sacccharomycetales
Archiascomycetes
Basidiomycetes
Characterized by DNA sequence analysis
Ascocarps; ascogenous hyphae; specialized ascus tip; conidia; Woronin bodies
Absence of ascogenous hyphae and ascocarps; most asci without specialized tips
Classification from Alexopoulos et al. 1996
![Page 5: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/5.jpg)
RAGI
Ascomycetous yeastsCharacterized by absence of ascogenous hyphae and ascocarpsAsci mostly prototunicateOccur in slime fluxes, nectar, fresh or decaying fruit—able to grow in high osmotic conditions (high sugar content)Others occur in soil, dung, water, digestive tracts of animalsMany species are symbiotic with insects
![Page 6: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/6.jpg)
Schizosaccharomyces
![Page 7: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/7.jpg)
Saccharaomyces
![Page 8: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/8.jpg)
Somatic structures
Yeast A single-celled fungus
that reproduces by budding (or fission)
Pseudomycelium Series of cells
adhering after budding
![Page 9: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/9.jpg)
Budding
Bipolar Multilateral
![Page 10: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/10.jpg)
Asexual reproduction
ConidiaArthrospores
![Page 11: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/11.jpg)
plasmogamy
copulation
karyogamy
budding
2n somatic cells
meiosis
Mature ascus
1n somatic cells
![Page 12: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/12.jpg)
Identification
Microscopic appearance Unicellular or budding Size & shape of yeast cells Multilateral or bipolar budding Form, structure and mode of ascus formation Ascospore shape
![Page 13: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/13.jpg)
Identification
Physiological tests—91 different tests Ferment different sugars Assimilation tests (carbon and nitrogen
source) Vitamin requirements Growth at 37C Growth in cyclohexamide Hydrolyse urea Form acid
![Page 14: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/14.jpg)
Importance
BrewingBakingFood productionIndustrial applicationsModel systems (S. cerevisiae)ProbioticsAnimal pathogens
![Page 15: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/15.jpg)
MICROSCOPIC (NON-YEAST)
![Page 16: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/16.jpg)
From Hanlin, 1998. Illustrated Genera of Ascomycetes Vol II
Ascospore by D. Geiser
•Aspergillus anamorph•Cleistothecia yellow to orange-red•Wall composed of single layer of flattened cells• Ascospores flattened, usually with equatorial groove.
Eurotium
![Page 17: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/17.jpg)
Aspergillus anamorphCleistothecial wall surrounded by hülle cellsAscospores small, colored, lens-shaped with flange
From Hanlin, 1998. Illustrated Genera of Ascomycetes Vol II
Emericella
![Page 18: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/18.jpg)
Emericella
Hülle cells, D. Geiser
![Page 19: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/19.jpg)
Anamorphs--Aspergillus
SEM by Charles Mims
![Page 20: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/20.jpg)
Penicillium anamorphCleisothecia hard, white becoming colored (yellow, orange, brown)Ascospores small, hyaline or yellowish, lens-shaped
From Hanlin, 1998. Illustrated Genera of Ascomycetes Vol II
Eupenicillium
![Page 21: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/21.jpg)
Image by David Geiser
![Page 22: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/22.jpg)
Anamorphs--Penicillium
phialides
Branches (metulae)
![Page 23: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/23.jpg)
From Hanlin, 1998. Illustrated Genera of Ascomycetes Vol II
TalaromycesPaecilomyces or Penicillium anamorphCleistothecium whitish to bright yellowWall composed of interwoven hyphaeAscospores ellipsoidal, with spiny walls
![Page 24: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/24.jpg)
Anamorphs--Paecilomyces
Divergent phialides with swollen base and long, tapering neck
Colonies may be pink, purple, yellow, brown or white, but never green as in Penicillium spp.
![Page 25: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/25.jpg)
The good and the bad
Penicillium spp.—antibiotic productionPenicillium roqueforti—blue cheesePenicillium spp.—blue and green molds on bread, cheese, fruits, vegetablesAspergillus flavus—aflatoxins (moldy peanuts)A. flavus/A. niger--aspergillosis
![Page 26: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/26.jpg)
![Page 27: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/27.jpg)
PenicillinPenicillium notatum growing in Alexander Fleming’s Petri dish of Staphylococcus in 1928 led to the discovery of penicillinHoward Florey & Ernest Chain (1939) began work on purification and trials1941—work moved to US (NRRL in Peoria, IL) to escape bombing in London (WWII)
Fermentation vessels and corn steep liquor Mary Hunt (“Moldy Mary”) brought in P. chyrogenum
on a melon1945—Fleming, Florey & Chain received Noble Prize
![Page 28: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/28.jpg)
Penicillium notatum
Penicillin prevents cross-linking of small peptide chains in peptidoglycan, the main wall polymer in bacteria. Newly formed cells are abnormal in shape and susceptible to osmotic lysis.
![Page 29: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/29.jpg)
Fungal taxon/group Est. # species # known metabolites
Aspergillus, Eurotium, Emericella 200 525
Penicillium, Talaromyces, Eupenicillium 200 380
Trichoderma, Hypocrea 20 54
Cephalosporium-like hyphomycetes 140 116
Mucor, Rhizopus, Phycomyces 70 26
Oomycetes, Chytridiomycota 450 3
Yeasts 600 50
Basidiomycetes 30,000 300
![Page 30: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/30.jpg)
POWDERY MILDEW
Biotrophs of vascular plants Biotroph: an obligate parasite growing on another
living organism21 genera, 437 species infecting > 40,000 species of plants (mostly dicots)Most species are host specific, a few are omnivorous, infecting hundreds of host species
![Page 31: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/31.jpg)
Powdery Mildew Symptoms
Photo by Claudia Nitschwitz
![Page 32: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/32.jpg)
CharacteristicsMycelium is mostly superficial
Anchored to host epidermis by appressoriaNutrients obtained via haustoria
Haustoria are intracellular structuresOverwinter as mycelium in infected buds or as ascomataAsexual reproduction via conidiaSexual reproduction via ascospores formed in cleistothecia
![Page 33: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/33.jpg)
Asexual reproduction
Erect, hyaline conidiophores are usually formed on superficial mycelium;One-celled, hyaline thin-walled conidia are produced holoblastically in basipetal chainsOne colony can produce > 30,000 conidia
![Page 34: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/34.jpg)
Conidia
Wind-dispersedGermination can occur at low relative humidityGermination involves germ tube, appressorium and penetration peg formationApex of penetration peg enlarges to form haustorium
![Page 35: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/35.jpg)
From APSnet.org
![Page 36: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/36.jpg)
Plant cell wallPlasma membrane
Host cytoplasm
Plasma membrane
haustorium
appressorium
Penetration peg
fungus
![Page 37: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/37.jpg)
Microsphaera alni anamorph on Vaccinium
![Page 38: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/38.jpg)
Sexual reproduction
Cleistothecia formed on superficial mycelium in late summer/early fallAsci Formed in basal layer Globose to pyriform Discharge of spore by rupture of ascus tip
![Page 39: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/39.jpg)
Asci/Ascospores
One to numerous asci/cleistotheciumAscospores hyaline, one-celled, ovoid1-8 ascospores/ascusNumber of asci/cleistothecium is important character in identification
![Page 40: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/40.jpg)
From APSnet.org
![Page 41: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/41.jpg)
Identification
Anamorph typeNumber of asci/ascocarpCleistothecial appendages Mycelioid Rigid
Spear-like with inflated baseWith curled tipsWith dichotomously branched tips
![Page 42: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/42.jpg)
Microsphaera alni cleistothecia
![Page 43: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/43.jpg)
Sawadaea bicornis cleistothecia
![Page 44: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/44.jpg)
Sawadaea bicornis cleistothecial appendages
![Page 45: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/45.jpg)
Mycelioid AppendagesSeveral asci/ascocarp:
Eryisiphe (100 spp)Oidium anamorph
One ascus/ascocarp: Sphaerotheca (50 spp.)
Appendages with curled tipsOidium anamorph
![Page 46: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/46.jpg)
Dichotomously branched appendage tips
One ascus/ascocarp: Podosphaera (12 spp.)
Oidium anamorph
Several asci/ascocarp:
Sawadaea (6 spp.)Oidium anamorph
![Page 47: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/47.jpg)
Spear-like appendages--Phyllactinia
Ovulariopsis and Streptopodium anamorphs
![Page 48: Ascomycota (continued) MIKROSKOPIK](https://reader035.fdocuments.us/reader035/viewer/2022081420/56816722550346895ddba999/html5/thumbnails/48.jpg)
Appendages with curled tips
Uncinula (81 spp) Oidium anamorph Several asci/ascocarp