SUPPORTING DATA

SFig. 1 Expression analysis of HSP-70 specific mRNA transcripts during proliferation and differentiation of human neural precursor cells. The gene expression analysis revealed that chronic ethanol exposure to cells tend to enhance the expression of selected HSP transcripts in both the conditions which shows the abnormality in protein folding. Although single treatment strategies using either VPA or MH slightly enhances the expression of these transcripts, however do not provide complete defence against the ethanol-induced damage. Combination of VPA+MH showed significantly high upregulation in the expression of the HSP-70 transcripts as compared to the ethanol (***p<0.0001) which reveals the maximum protection by inducing HSP-70 expression to confer the normal protein folding during abnormal events

SFig. 2 Gene expression analysis of HDAC mRNAs of different categories to reveal their involvement in neurological cells during the events of ethanol-induced damage and normalization post-treatment with VPA and MH. All the selected HDAC sub-types showed normalized expression levels as compared to the control condition (ns: non-significant) except HDAC6 and HDAC10 which did not change its expression levels after treatment with VPA, MH or VPA+MH as compared with the ethanol exposure (non-significant), whereas in comparison to control condition their expression levels was significantly enhanced and was similar to the ethanol condition (***p<0.0001)

SFig. 3 Schematic representation showing the cellular and molecular pathways involved in neurological protective mechanisms using MH+VPA against ethanol-induced damage