Data: 24/02/2014

Aflatoxin Prevalence in the World

Percentage of positive samples

North & Central America30%

South America18%

Africa80% South &

Central Asia73%

Middle East35%

Southern Europe44%

Northern Europe19%

Eastern Europe71%

Oceania2%

South-East Asia57%

North Asia10%

Why choose ZeniFIX?

ZeniFIX is a specially selected Hydrated Sodium Calcium Aliminosilicates (HSCAS)

blend, with high efficiency to adsorb polar mycotoxins such as Aflatoxins.

ZeniFIX possess ultra laminated and porous structure, building up a large surface

area which leads to maximum adsorption capacity.

ZeniFIX is mined in the Andes, the world’s best source for HSCA.

The Andes Mountain Range

The Andes are one of the largest mountain ranges in the world, stretching through the

whole north-south length of South America. The Andean mines are known to produce

the highest quality HSCAS products.

ZeniFIX is extracted from the Andes in Argentina, an area sparsely populated and with

no industrial production, thus zeroing the risk of dioxins and other industrial

contaminants to be present in the product.

Heavy metals and dioxins are major concerns associated to HSCAS products.

ZeniFIX is guaranteed to contain low levels of heavy metals and it is free from dioxins.

ZeniFIX is regularly analysed for the presence of contaminants in accordance to

international standards, ensuring its safety.

ZeniFix: Powerful against Mycotoxins,

Harmless against Vitamins!

The Cation Exchange Capacity (CEC) is one factor that indicates whether a product is a

good adsorbent. A low CEC means that the product cannot bind mycotoxins, with a

too high CEC you might catch nutrients from the diets.

Some HSCAS can present a high CEC; many products adsorb important nutrients such

as minerals and vitamins which might impair the animals growth.

ZeniFIX CEC is controlled in order to guarantee its adsorption efficacy without

catching the feed nutrients:

ZeniFIX CEC

58~100 Meq/100g

Mode of Action

The calcium and sodium molecules from

Aluminum Silicates will bind to the

mycotoxins structure, being later

eliminated.

This linkage is strong and will resist to

variations of temperature and pH in the

gut.

ZeniFIX is indigestible and the

mycotoxins will be excreted with the

feces.

Blend of Specially Selected Aluminum Silicates

Ca++

Interlaminarcations

Aflatoxinstructure

Na+

ZeniFIX

Typical Chemical Analysis:

SiO2 62.60 %

Al2O3 15.86 %

Fe2O3 2.01%

CaO 2.10 %

MgO 3.01 %

Na2O 2.15 %

K2O 0.48 %

Moisture: 10.50 %

ZeniFIXRecommendation to all species:

Preventive: 1.0 kg/ton of feed

Under Challenge: 2.0 kg/ton of feed

ZeniFix & ImmunoWall combination:

ImmunoWall = 0.5 kg/ton ---> ZeniFix - 1.0 kg/ton

ImmunoWall = 1.0 kg/ton ---> ZeniFix - 0.5 kg/ton

Data: 03/04/2014

In vitro Trials

Determination of “in vitro” Mycotoxin Adsorption Laboratory Technique

Determination of “in vitro” Mycotoxin

Adsorption Laboratory Technique

Method: Chromatografy

Determination of toxins (TLC).

Determination of pattern sample.

Determination of blanks.

Sample incubation with ZeniFIX at 36º C., pH. 4.

Desorption in time.

Adsorption according to concentration.

TraceabilityZeniFIX Adsorbing Power

Average results of successive tests carried out in 1998

Based on production samples and users.

98%

95%93%

95%97%

94%

98%96% 95% 95%

97%95%

98%

95%

50%

60%

70%

80%

90%

100%

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sample

Data: 03/04/2014

In vivo TrialsField Experimental Tests

Use of HSCAS on Broiler Feeding

INTA – Instituto Nacional de Tecnología Agropecuaria

Pergamino, Argentina

1997

Analysis & Methods

60 male and 60 female day-old chicks Arbor Acres.

Challenge: Naturally contaminated maize.

4 treatments with 8 replicates:

Control.

Control + ZeniFIX– 2kg/ton.

Aflatoxin.

Aflatoxin + ZeniFIX– 2kg/ton.

Trial Period: January ~ March.

Feed Formula

Diet # 1 2 3 4

A. Met. E. (kcal) 3050 3150 3200 3200

Protein (%) 21.00 20.00 18.00 17.00

Methionine (%) 0.50 0.45 0.40 0.40

M+C (%) 0.94 0.87 0.80 0.70

Lysine (%) 1.10 1.00 0.90 0.90

Total aflat. (ppb) 30 27 36 28

Average Weight (g)

Treatment 21 days 35 days 49 days

Without afla.

Without ZeniFIX

783a 1808 2910a

Without afla.

With ZeniFIX

769b 1818 2930ª

With afla.

Without ZeniFIX

757b 1796 2864b

With afla.

With ZeniFIX

789a 1827 2909a

Data: 03/04/2014

Study on the Efficacy of Mycotoxin Brinder for Broiler Feeds

Alimentos Procria

Venezuela

1998

In vivo Trials

Analysis & Methods

50 male and 50 female day-old chicks Arbor Acres.

Challenge: Naturally contaminated maize.

2 treatments with 8 replicates:

Aflatoxin.

Aflatoxin + ZeniFIX – 2kg/ton.

Trial Period: September ~ December.

Average Weight (g) & Feed

Conversion

Average weight Conversion

Treatment 21 days 49 days 21 days 49 days

Without ZeniFIX 597 g 2238 g 1.423 1.956

With ZeniFIX 626 g 2297 g 1.402 1.878

Two foods with 28 and 37 ppb aflatoxins were used. Ocratoxin was not found.

Data: 24/02/2014

Evaluation on Broiler’s Performance when Fed a Control Diet or HSCAS

Diet without Mycotoxins Contamination

Alimentos Procria

Venezuela

1998

In vivo Trials

Analysis & Methods

60 male and 60 female day-old chicks Arbor Acres.

Challenge: All feeds are free of mycotoxins.

2 treatments with 20 replicates:

Control.

Control + Zenifix – 2kg/ton.

Trial Period: January ~ March.

Average Weight (g)

Treatment Day 21 Day 35 Day 42 Day 49

Without ZeniFIX 652 1134 2066 2406

With ZeniFIX 675 1121 2096 2456

Feed Conversion(g/g)

Treatment Day 21 Day 49

Without ZeniFIX 1.486 1.978

With ZeniFIX 1.484 1.986