Applied Biosystems and Dr. William Farmerie A partnership for the advancement of science

Founded in 1988 by the Florida state legislature, the Interdisciplinary Center for Biotechnology Research (ICBR) is a research support organization which provides high-end technical services to the research community at the University of Florida, as well as across the State of Florida and beyond.

We recently had the opportunity to talk with Dr. Bill Farmerie, ICBR’s Associate Director, about how the Center is using the Applied Biosystems® SOLiD™ System to help researchers find new ways to ask questions and interpret data.

Tell us a little about the purpose of the ICBR. The ICBR was founded around 1988 as a research-support organization. It consists of four major divisions—Cellomics, Proteomics, Bioinformatics, and Genomics—whose research support laboratories provide more than 250 technical services as diverse as electron microscopy, flow cytometry, mass spectrometry, genetic analysis, and, next-generation high-throughput DNA sequencing. University of Florida (UF) has a very diverse scientific community spanning from medicine to agriculture. If you were going to set up a DNA sequencing operation, you would really have a hard time picking a better place than the University of Florida to do it.

What areas of research is the ICBR engaged in?We have a medical school, a dental school, a college of pharmacy, and a school of veterinary medicine, which means we have research scientists working in many areas relevant to human and animal health. We are also home to the Florida Museum of Natural History, and UF is also Florida’s land grant institution, so we have a very large agricultural presence both on campus and in a network of labs located throughout the state.

Our labs also do work for the biotechnology industry in the state of Florida and for other universities around the country and around the world. Many scientists want to collaborate with UF faculty in order to gain access to the expertise ICBR brings and the leading-edge technology we provide here. So the short answer is, we are involved in pretty much everything—from medically based research to crop science, animal science, and veterinary science. It’s really a very fertile place to be doing this kind of work.

EMPOWERING THE ADVANCEMENT OF SCIENCE, ONE PARTNERSHIP AT A TIME

Advancing ScienceApril 2010 Quarterly Newsletter

North America Training ProgramsMolecular & Cell Biology | Human Identification

IN THIS ISSUE:

Featured Collaboration: Applied Biosystems and Dr. William FarmeriePage 1–3

First 3500 Series Genetic Analyzer Training Class Successfully Completed Page 4

New Training Available for SOLiD™ 4 System & SOLiD™ EZ Bead System Page 5

Attune™ Power Hour Upcoming at New Application Support Center Pages 6

CE/Sequencing Seminars Bring Equipment, Expertise to the Southeast Page 6

Course Calendar, 2010 Pages 7–10

Special Offer: Frederick Application Support Center TrainingPage 11

Advancing Science North America Training Programs

To learn more, visit learn.appliedbiosystems.com/na.2

How important is next-generation sequencing to the ICBR and the research you conduct here?

We made the decision to adopt next-generation sequencing very early on, when the first instruments were just arriving on the market. Right away, it had a tremendous impact on productivity and cost efficiency, and essentially transformed how we thought about doing life sciences research. After a couple of years, new short-read instruments that had a completely different impact from the original instruments became available, and allowed us to expand the scope of projects we could do for our clients.

Today, next-generation sequencing is critically important to ICBR because it is a broadly enabling technology with critical importance to the research community. Our role in the university is to identify transformative technologies and make them available. The way our research community has embraced it is actually quite amazing as well as gratifying.

What are the major considerations the Center has to weigh when adopting a new technology like a next-generation sequencing system?

As a service facility, it is just as important to be able to add value to raw data after it streams out of the machine, as it is to acquire the data in the first place. So, when we’re planning for the future, one of the things we spend the most time thinking about is how we’re going to stay ahead of the curve and how we’re going to keep up with increasing demands placed on information management and data analysis systems. Because once you put a technology in the hands of the research community, they immediately begin to find new ways to use it.

Each next-gen application, and there is an ever expanding range of applications, has its own set of computational requirements for interpreting the raw data. It’s computation that gives raw sequence data its meaning, and if we can’t handle the volume of data we generate with these instruments, then we’re done. We’re toast. That’s what it’s all about—the research world will be divided into those who can do the computational work and those who can’t. So that’s one of the things we press here: “Do we want to be able to do sequencing and computation or do we want to sit back and watch everyone else do it?”

Why did you choose the Applied Biosystems SOLiD™ system over other next-generation sequencing systems?

We were comfortable with the idea of next generation sequencing through our experience with an earlier platform, but it was becoming clearer there was an entirely new sphere of applications for instruments that produced hundreds of millions of short sequence tags. We started a dialog with Applied Biosystems not long after they acquired SOLiD™ technology and I was immediately intrigued by the notion of two base encoding. I felt single nucleotide polymorphism (SNP) hunting was certainly one of the applications we would use this for, and the accuracy of the SOLiD™ System was very appealing.

Furthermore, Applied Biosystems is just technically superb. They know what they’re doing and they have a great reputation for keeping their promises when it comes to bringing new technology to the research community—that’s essential in a remarkably fast-moving field like DNA sequencing. I was also impressed with their very workable strategy for doing mate-pair sequencing, which I thought was going to be a really big feature—and I was right!

William G. Farmerie, Ph.D. Associate Director Interdisciplinary Center for Biotechnology Research, University of Florida

April 2010

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How would you describe your collaborative relationship with the SOLiD™ Sequencing team at Applied Biosystems?

Applied Biosystems has been wonderfully supportive. As one of the early adopters of the technology, we had constant contact with SOLiD™ System technical staff for training, troubleshooting, and generally working our way through the process. The high level of customer service was key, because the technology was so new that we couldn’t anticipate all the issues that might arise. But whenever we put a question to them, they came right back with an answer. They helped us get up to speed quickly to where we could use the instrument the way it’s intended to be used and start pushing the applications envelope into the future.

Can you give us some examples of the kind of projects you’re doing on the SOLiD™ System here at the ICBR?

One of the first things we were approached about doing is a technique called ChIP-Seq, which is used among other applications, for looking at specific protein interactions in chromatin in order to identify regions of the genome that are being expressed. We also worked on bacterial genome resequencing, which involved looking at serial isolates of a bacterial species having variant phenotypes.

Recently, we’ve been doing a lot of human genome resequencing using mate-pair libraries, because the cancer research community is intensely interested in looking at genomic rearrangements in various kinds of cancers. We have some exciting new possibilities for our work with Moffitt Cancer Center in Tampa, as well as our own cancer center, that are driven by the new applications for SOLiD™ Sequencing.

There’s also huge buzz right now about RNA–Seq, and I think that will be the focus of the next set of projects for the SOLiD™ System.

What excites you most about the work you’re doing at the ICBR?Our role in the research process is to provide individual investigators with the technology, the technical expertise, and the computational infrastructure required to produce and interpret raw DNA sequence data. They can come to us with an idea and we help find ways to apply technology to the question at hand. It’s very satisfying to enable technology that helps scientists arrive at that “Eureka” moment, where they make a discovery or solve a mystery they’ve spent a part of their career working on.

That’s what makes this business so darn exciting—it is constantly evolving and you just have to ride the wave, because there is always something bigger, faster, and cheaper just over the horizon. What we can do today is just scratching the surface of what we will be doing tomorrow or next week or next year—that’s how fast these instruments are advancing. I love being able to bring new capabilities to the research community and participate in so many different kinds of projects. I can’t imagine doing something more exciting than playing this particular role.

What direction do you see your future work with the SOLiD™ System taking?

The research community is prospecting for funding to do some pretty exciting projects. There’s no question that the possibilities for medical resequencing are pretty much limitless, and there is tremendous interest in the instrument from the medical community, especially cancer researchers. We’re also seeing a lot more happening on the agricultural side especially as additional genome sequences emerge, so I’m confident that we are going to be using this technology across the board.

I also think a major new area of research will center on exploring the intricacies of the transcriptome. We really don’t appreciate the full extent of the diversity of transcribed molecules. But with the SOLiD™ System we have technology to probe gene expression at a completely new level.

Advancing Science North America Training Programs

To learn more, visit learn.appliedbiosystems.com/na.4

First 3500 Series Genetic Analyzer Training Class Successfully CompletedNew customers of the 3500 Series Genetic Analyzer recently completed the first training session offered for these instruments. Researchers from both UCSF and Kaiser Permanente were among those that participated in the training that took place at our Application Support Center in Foster City, CA. The hands-on, three-day intensive study of DNA sequencing and fragment analysis applications included an overview of 3500 instrument hardware, application workflow setup and optimization, operation and maintenance, use of data collection software, preparation and running of samples and standards, analysis software tutorials, and troubleshooting discussions.

Nancy Bouvier, a research associate at UCSF, said, “The 3500 Systems Training course was great; the review sessions in the morning were really effective and both trainers did a great job explaining the instrument in an understandable language. I have a lot of confidence coming out of the training to run and maintain the instrument by myself.” The successful training session was capped off with a celebration sponsored by the Applied Biosystems marketing department where the attending customers shared their 3500 instrument and applications experiences with an attentive product management team.

“The 3500 Systems Training course was great; the review sessions in the morning were really

effective and both trainers did a great job explaining the instrument in an understandable language.

I have a lot of confidence coming out of the training to run and maintain the instrument by myself.”

Nancy Bouvier Research Associate UCSF

For more information, on 3500 Series Genetic Analyzer training, please visit learn.appliedbiosystems.com/fragmentanalysis or contact your local Applied Biosystems sales representative.

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New Training Available for SOLiD™ 4 System & SOLiD™ EZ Bead™ SystemThe SOLiD™ 4 System enables researchers to obtain higher-quality genomes at lower costs. Researchers can now experience peace of mind knowing that they can upgrade without the purchase of a new instrument. Continually pushing technological boundaries, the SOLiD™ 4 System delivers:

• $6,000 genome with 100 GB of mappable data

• Increases in accuracy and coverage uniformity

• Sample prep automation

• Multiplexing of up to 96 samples to accelerate scientific discovery

The SOLiD™ EZ Bead™ System reduces your hands-on timeby 80%, providing an automated solution for reproducible templated bead preparation with less than 1 hour of hands-on time. The system modularity and quality control checkpoints provide greater experimental control. Additional support for multiplexed libraries further maximizes time and cost savings.

We’re pleased to announce a variety of training options for these systems. These options include:

• 5-day Systems Training at an Applied Biosystems training facility

• 5-day Libraries Training at an Applied Biosystems training facility

• 2-day Bioinformatics Training at an Applied Biosystems training facility

• Series of e-learning modules available online

For more information, please visit https://learn.appliedbiosystems.com/solid or contact your local Applied Biosystems sales representative.

A variety of new web-based training modules are available for the new SOLiD™ 4 System. These options, which typically consist of 45-minute courses followed by 15-minute Q&A sessions, will include the following:

SOLiD™ 4 System Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customersThis is an online training session on the SOLiD™ 4 System designed for customers who are currently using the SOLiD™ 3 or SOLiD™ 3 Plus systems. The goal of this training is to highlight changes and improvements to the SOLiD™ System Library, ePCR, and instrument.

SOLiD™ 4 Data Analysis Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers

This is an online training session on the SOLiD™ 4 System designed for customers who are currently using the SOLiD™ 3

System. The goal of this training is to highlight changes and improvements to the SOLiD™ Data Analysis workflow. This training will cover SOLiD™ Data Analysis from on-instrument data processing in ICS and SETS to off-instrument analysis using BioScope including an overview of software community tools for application-specific analysis.

Automated Bead Preparation with SOLiD™ EZ Bead™ System

This is an online training session on the new automated solution for bead preparation for SOLiD™. You will learn how to prepare emulsions, run PCR, and enrich beads with the 3 EZ Bead™ modules.

STAY TUNED for SOLiD™ self-paced e-learning available in May!

NEW SOLiD™ Edge Online Learning Series

Advancing Science North America Training Programs

To learn more, visit learn.appliedbiosystems.com/na.6

Please mark your calendar for a special customer event introducing the Applied Biosystems® Attune™ Acoustic Focusing Cytometer the first cytometer on the market to use sound waves to manipulate cells for single-cell analysis. Attune™ brings significant performance advantages compared to traditional flow cytometry. Incorporating breakthrough acoustic focusing technology allows users the ability to quickly process dilute samples and to exquisitely control transit times in the laser beam, yielding increased sensitivity without sacrificing throughput. As a result, the Attune™ Cytometer excels at rare event detection and provides lower signal variation in populations. The Attune™ Acoustic Focusing Cytometer will fit in a conventional laminar flow hood, requires no fluid cart, and provides intuitive software control and data analysis functions.

We’ll be holding this special event at our Application Support Centers in Frederick, MD, and Foster City, CA. These sessions will be the first in a series of “Advancing Science Power Hours” that will feature a technical talk and demonstration followed by a catered reception. Please join us in this opportunity to learn about the latest developments in flow cytometry, mingle with our experts, and see our state-of-the-art training facilities.

For more details and to register for this event, please

In late January, experts from Applied Biosystems held two well-received half-day seminars, in Atlanta, GA, and Durham, NC, on the latest developments in DNA sequencing. While being served continental breakfast and box lunches, over 75 attendees were treated to presentations and literature covering the 3500 Series Genetic Analyzer, the new SOLiD™ 4 System, and the Sequence Manipulation Suite (SMS). Guest speaker Dr. Michael Friez from the Greenwood Genetics Center also spoke on the subject of clinical applications with capillary electrophoresis (CE). Additional presentations covered the FAST

sequencing workflow and CE applications and troubleshooting. All of the presentations generated insightful questions from the audience, which, by and large, found the seminars quite informative and worthwhile.

To find out about regional training near you, please visit www.learn.appliedbiosystems.com/calendar or contact your local Applied Biosystems sales representative.

CE/Sequencing Seminars Bring Equipment, Expertise to the Southeast

Attune™ Power Hour Upcoming at Application Support Centers

visit: www.learn.appliedbiosystems.com/powerhour or contactyour local Applied Biosystems sales representative. To learn more about Attune™, please visit: www.appliedbiosystems.com/attune

What: Attune™ Demonstration and Customer Reception

Where/When: East Coast Session: Tuesday, April 27, 4:00 p.m. Life Technologies Application Support Center 7335 Executive Way Frederick, MD 21704

West Coast Session: Tuesday, May 25, 4:00 p.m. Applied Biosystems Application Support Center 850 Lincoln Centre Drive, Building 600 Foster City, CA 94404

April 2010

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3500 Systems Training (3.5 days) April 19–22, 9:00 a.m.–5:00 p.m. P/N: TRN00127

SOLiD™ 4 System Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) April 20, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the 7500 (2 days)April 20–21, 9:00 a.m.–5:00 p.m.. P/N: N020-0546

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the StepOne™/StepOne Plus™ (2 days)April 20–21, 9:00 a.m.–5:00 p.m. FREE

GeneMapper® v4.0 Introductory Software Training (1.5 hours) April 20, 9:00–10:30 a.m. P/N: TRN00127

SOLiD™ BioInformatics Course (2 days) April 28–29, 8:00 a.m.–5:00 p.m. P/N: TRN00125

SOLiD™ 4 System Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) April 27, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

Virtual Office Hours: Introduction to the new Applied Biosystems® 3500 and 3500xL Genetic Analyzer (1 hour) April 28, 10:00–11:00 a.m. FREE SOLiD™ 4 System Essentials for SOLiD™ 3 and SOLiD™ 3 Plus CustomersMay 4, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

Real-Time Quantitative PCR Training Series: Theory of Real-Time Quantitative PCR Analysis (6 x 1 hour) May 7, 9:00–10:00 a.m. FREE

7500 2.0 Software and Data Analysis Session (1 hour) May 7, 11:00 a.m.–12:00 p.m. FREE

Real-Time Quantitative PCR Training Series: Data Analysis Using the 7900HT Software (6 x 1 hour) May 7, 12:00–1:00 p.m. FREE

SOLiD™ Systems Training (1 week) May 10–14, 8:00 a.m.–5:00 p.m. P/N: TRN00069

SOLiD™ 4 System Essentials for SOLiD™ 3 and SOLiD™ 3 Plus Customers (1 hour) May 11, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

200 Forensic DNA Analysis Training (Quantification to Interpretation) (4 days) May 11–14, 8:30 a.m.–5:00 p.m. P/N: TRN00087

Gene Expression Applications Course (3 days) May 11–13, 9:00 a.m.–5:00 p.m. P/N: TRN00162

SOLiD™ DNA Library Construction (1 week) May 17–21, 8:00 a.m.–5:00 p.m. P/N: See online course description

SOLiD™ 4 Data Analysis Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) May 18, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the StepOne™/StepOne Plus™ (2 days) May 18–19, 9:00 a.m.–5:00 p.m. P/N: TRN00058

Course Calendar 2010

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To learn more, visit learn.appliedbiosystems.com/na.8

300 Advanced GeneMapper® ID-X Software Training (3 days) June 8 – 10, 8:30 a.m.–5:00 p.m. P/N: TRN00085

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the 7900 (2 days)June 7–8, 9:00 a.m.–5:00 p.m. P/N: N020-0529

Automated Bead Preparation with SOLiD™ EZ Bead™ SystemJune 15, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

SOLiD™ Systems Training (1 week) June 21–25, 8:00 a.m.–5:00 p.m. P/N: TRN00069

310 Advanced HID Training: Working with Difficult Samples (4 days) June 22–25, 8:30 a.m.–5:00 p.m. P/N: TRN00097

SOLiD™ 4 Data Analysis Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) June 22, 9:00–10:00 a.m. and 5:00–6:00 p.m. FREE

Applications Training for Fragment Analysis (3 days) June 22–24, 9:00 a.m.–5:00 p.m. P/N: TRN00052

GeneMapper® v4.0 Introductory Software Training (1.5 hours) June 22, 9:00–10:30 a.m. FREE

SOLiD™ BioInformatics Course (2 days) June 23–24, 8:00 a.m.–5:00 p.m. P/N: TRN00125

SOLiD™ RNA Seq Library Construction (1 week) June 28–July 2, 8:00 a.m.–5:00 p.m. P/N: See online course description

SOLiD™ Systems Training (1 week) July 12–16, 8:00 a.m.–5:00 p.m. P/N: TRN00069

TaqMan® SNP Genotyping, High Resolution Melt and Copy Number Variation (3 days) July 13–15, 9:00 a.m.–5:00 p.m. P/N: TRN00128

Course Calendar (continued)

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the StepOne™/StepOne Plus™ (2 days)May 18–19, 9:00 a.m.–5:00 p.m. P/N: TRN00058

SOLiD™ Systems Training (1 week)May 24–28, 8:00 a.m.–5:00 p.m. P/N: TRN00069

SOLiD™ 4 Data Analysis Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) May 25, 9:00–10:00 a.m. and 5:00 – 6:00 p.m. FREE

TaqMan® SNP Genotyping, High Resolution Melt and CopyNumber Variation (3 days) May 25–27, 9:00 a.m.–5:00 p.m. P/N: TRN00128

Applications Training for Sequencing (3 days) May 25–27, 9:00 a.m.–5:00 p.m. P/N: TRN00053

Advanced GeneMapper® v4.0 Software Training (1.5 hours)May 25, 9:00–10:30 a.m. FREE Automated Bead Preparation with SOLiD™ EZ Bead™ SystemJune 1, 9:00 –10:00 a.m. and 5:00–6:00 p.m. FREE

Real-Time Quantitative PCR Training Series: Fundamentals of Real-Time PCR (6 x 1 hour) June 4, 9:00–10:00 a.m. FREE

Real-Time Quantitative PCR Training Series: Real-Time PCR Assay Design Choices (6 x 1 hour) June 4, 12:00–1:00 p.m. FREE

SOLiD™ Systems Training (1 week) June 7–11, 8:00 a.m.–5:00 p.m. P/N: TRN00069

SOLiD™ 4 Data Analysis Essentials for SOLiD™ 3 and SOLiD™ 3 Plus customers (1 hour) June 8, 9:00–10:00 a.m. and 5:00 –6:00 p.m. FREE

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SOLiD™ Targeted Resequencing Library Construction (1 week) August 16–20, 8:00 a.m.–5:00 p.m. P/N: See online course description

SOLiD™ Systems Training (1 week) August 23–27, 8:00 a.m.–5:00 p.m. P/N: TRN00069

200 Forensic DNA Analysis Training (Quantification to Interpretation) (4 days) August 24–27, 8:30 a.m.–5:00 p.m. P/N: TRN00087

Applications Training for Sequencing (3 days)August 24–26, 9:00 a.m.–5:00 p.m. P/N: TRN00053

SOLiD™ Targeted Resequencing Library Construction (1 week)August 30–September 3, 8:00 a.m.–5:00 p.m. P/N: See online course descripton SOLiD™ Systems Training (1 week) September 13–17, 8:00 a.m.–5:00 p.m. P/N: TRN00069

300 Advanced GeneMapper® ID-X Software Training (3 days) September 14–16, 8:30 a.m.–5:00 p.m. P/N: TRN00085

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the 7900 (2 days)September 14–15, 9:00 a.m.–5:00 p.m. P/N: N020-0529

SOLiD™ DNA Library Construction (1 week) September 20–24, 8:00 a.m.–5:00 p.m. P/N: See online course description

TaqMan® SNP Genotyping, High Resolution Melt and Copy Number Variation (3 days) September 21–23, 9:00 a.m.–5:00 p.m. P/N: TRN00128

Applications Training for Fragment Analysis (3 days) September 21–23, 9:00 a.m.–5:00 p.m. P/N: TRN00052

Course Calendar (continued)

Gene Expression Applications Course (3 days) July 19–21, 9:00 a.m.–5:00 p.m. P/N: TRN00162

320 Advanced HID Troubleshooting (3 days)July 20–22, 8:30 a.m.–5:00 p.m. P/N: TRN00098

Advanced GeneMapper® v4.0 Software Training (1.5 hours) July 20, 9:00–10:30 a.m. FREE

3500 Systems Training (3.5 days) July 26-29, 9:00 a.m.–5:00 p.m. P/N: TRN00127

Basic Principles of Real–Time Quantitative PCR and Instrument Maintenance for the 7500 (2 days) July 27–28, 9:00 a.m.–5:00 p.m. P/N: N020-05 46

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the StepOne™/StepOne Plus™ (2 days)July 27–28, 9:00 a.m.–5:00 p.m. P/N: TRN00058

SOLiD™ BioInformatics Course (2 days) July 28–29, 8:00 a.m.–5:00 p.m. P/N: TRN00125

SOLiD™ RNA Seq Library Construction August 2–6, 8:00 a.m.–5:00 p.m P/N: See online course descripton

SOLiD™ Systems Training (1 week) August 9–13, 8:00 a.m.–5:00 p.m. P/N: TRN00069

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the 7500 (2 days) August 10–11, 9:00 a.m.–5:00 p.m. P/N: N020-0546

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the StepOne™/StepOne Plus™ (2 days) August 10–11, 9:00 a.m.–5:00 p.m. P/N: TRN00058

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Advancing Science North America Training Programs

To learn more, visit learn.appliedbiosystems.com/na.10

SOLiD™ RNA Library Construction (1 week) November 15–19, 8:00 a.m.–5:00 p.m. P/N: See online course description

TaqMan® SNP Genotyping, High Resolution Melt and Copy Number Variation (3 days) November 16–18, 9:00 a.m.–5:00 p.m. P/N: TRN00128

Applications Training for Sequencing (3 days) November 16–18, 9:00 a.m.–5:00 p.m. P/N: TRN00053

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the 7500 (2 days) November 16–17, 9:00 a.m.–5:00 p.m. P/N: N020-0546

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the StepOne™/StepOne Plus™ (2 days) November 16–17, 9:00 a.m.–5:00 p.m. P/N: TRN00058

310 Advanced HID Training: Working with Difficult Samples (4 days) November 30–December 3, 8:30 a.m.–5:00 p.m. P/N: TRN00097

SOLiD™ BioInformatics Course (2 days) December 1–2, 8:00 a.m.–5:00 p.m. P/N: TRN00125

SOLiD™ Systems Training (1 week) December 6–10, 8:00 a.m.–5:00 p.m. P/N: TRN00069

300 Advanced GeneMapper® ID-X Software Training (3 days) December 7–9, 8:30 a.m.–5:00 p.m. P/N: TRN00085

SOLiD™ Targeted Resequencing Library Construction (1 week) December 13–17, 8:00 a.m.–5:00 p.m. P/N: See online course description

Applications Training for Fragment Analysis (3 days) December 14–16, 9:00 a.m.–5:00 p.m. P/N: TRN00052

Basic Principles of Real-Time Quantitative PCR and InstrumentMaintenance on the 7900 (2 days) December 14–15, 9:00 a.m.–5:00 p.m. P/N: N020-0529

SOLiD™ BioInformatics Course (2 days) September 22–23, 8:00 a.m.–5:00 p.m. P/N: TRN00125

310 Advanced HID Training: Working With Difficult Samples (4 days) September 28–October 1, 8:30 a.m.–5:00 p.m. P/N: TRN00097

Gene Expression Applications Course (3 days) September 28–October 1, 9:00 a.m.–5:00 p.m. P/N: TRN00162

SOLiD™ Systems Training (1 week) October 11–15, 8:00 a.m.–5:00 p.m. P/N: TRN00069

3500 Systems Training (3.5 days) October 18–21, 9:00 a.m.–5:00 p.m. P/N: TRN00127

320 Advanced HID Troubleshooting (3 days) October 18–21, 8:30 a.m.–5:00 p.m. P/N: TRN00098

SOLiD™ Targeted Resequencing Library Construction (1 week) October 18–22, 8:00 a.m.–5:00 p.m. P/N: See online course description

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the 7500 (2 Days) October 19–20, 9:00 a.m.–5:00 p.m. P/N: N020-0546

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance on the StepOne™/StepOne Plus™ (2 days) October 19–20, 9:00 a.m.–5:00 p.m. P/N: TRN00058

SOLiD™ Systems Training (1 week) November 8–12, 8:00 a.m.–5:00 p.m. P/N: TRN00069

200 Forensic DNA Analysis Training (Quantification to Interpretation) (4 days) November 9–12, 8:30 a.m.–5:00 p.m. P/N: TRN00087

Gene Expression Applications Course (3 days) November 9–11, 9:00 a.m.–5:00 p.m. P/N: TRN00162

January 2010 continued

FC

FC

FC

FC

FC

FC

FC

FRD

FRD

FRD

FRD

November 2010 continued

December 2010

September 2010 continued

October 2010

November 2010

FC

FC

FC

FC

FC

FC

FC

FC

FC

FRD

FRD

FRD

FRD

FRD

Course Calendar (continued)

N!

N!

April 2010

11

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To find training enlightenment now, simply visit learn.appliedbiosystems.com.

With Applied Biosystems training, scientific enlightenment is at hand.Need to get up to speed fast on new equipment? Want to build your expertise in theory and in practice, stay on top of the latest technical developments, and accelerate your scientific productivity?

Relax. Applied Biosystems offers the training you need to see you through. From in-depth, hands-on instrument and applications training to self-paced courses, a full offering of flexible options can help you transcend the unknown—on-site in your lab, online, at more than 20 facilities worldwide, or at our simply sublime Application Support Centers in Foster City, California, and Rockville, Maryland.

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