Application of EoNPV Against Tea Looper Ectopis obliqua in ... · Anniversary Occurrence of...
Transcript of Application of EoNPV Against Tea Looper Ectopis obliqua in ... · Anniversary Occurrence of...
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Application of EoNPV Against Tea Looper Ectopis
obliqua in China
Tea Research Institute Chinese Academy of Agricultural Sciences
Qiang Xiao26-10-2010
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• One of the major pests of tea bushes in China• Occurs in most tea plantations• Zhejiang, Jiangsu, Hunan, Anhui Provinces
Ectropis obliqua Prout
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The Life Cycle
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• Feed on tea leaf
• Make tea bushes brown and bare
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Anniversary Occurrence of Ectropis obliqua
0500
100015002000250030003500400045005000
3 4 5 6 7 8 9 10 11 12
month
amou
nt /m
u
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• Main natural enemies ——parasitic wasp & parasitic fungi
• parasitic wasp • parasitic fungi
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• One of the major natural enemies• Very effective in the field
Ectropis obliquaNucleopolyhedrovirus (EoNPV)
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• Found in 1978
• Determined morphological and biological characters
• Analyzed gene sequence
• Researched to use in the field
EoNPV
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Control effect of EoNPV against tea looper at different concentration
0
20
40
60
80
100
15 75 150 300
Usage doses (108PIB/ha)
Mor
talit
y (%
)15 days after spraying20 days after spraying
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Control effect of EoNPV against the tea looper larvae of different instars
1 2 3 4
0
20
40
60
80
100M
orta
lity
(%)
Instars
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Control effect of EoNPV against tea looper at different temperatures in the field
0
20
40
60
80
100
19 21 25 29
Average Temperature (℃)
Mor
talit
y (%
)
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Morality of tea looper fed with two types of EoNPV formulation
0
20
40
60
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4 6 8 10 12 14Days after feeding Eo NPV (d)
Mor
talit
y (%
)
EoNPVEoNPV-BT
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• Established the better way of reproducing virus
• Found the commercial formulation
• Using methods in the field
• Be registered as bio-pesticide agent
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Applications of EoNPV
0
500
1000
1500
2000
2500
3000
appl
ied
acre
age
(ha)
1990 1999 2001 2003 2005 2007 2009
year
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Virulence of EoNPV in different geographic populations of tea looper
geographic populations
0
12
34
56
78
9
HangZhou
YiXing
QuZhou
YangZ
hou
WuHan
XiangFan
GaoQiao
700×
log
LC
50
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conclusion
• EoNPV against tea looper is effective
• EoNPV as a microbial biocontrol agent has be registered
• EoNPV agent is be using to control tea looper rapidly
• Research should focus on the field application
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