Appendix 4 HPLC Calibration Curves - Roberts Bank … 4 HPLC Calibration Curves This Page Left...

HEMMERA ENVIROCHEM INC.

ROBERTS BANK TERMINAL 2 – TECHNICAL DATA REPORT

BIOFILM ANNUAL VARIABILITY

307071-00790 : Rev 1 : 30 March 2015 Appendices

Appendix 4 HPLC Calibration Curves

This Page Left Intentionally Blank

Operator:ssv Timebase:HPLC Sequence:2012-July Page 1-4

12/05/2014 11:00 AM

Pigments- Calibration Curves

Sample Name: Injection Volume: 350.0

Vial Number: Channel: UV_VIS_1

Sample Type: STD Wavelength: n.a.

Control Program: PDA Pigments-3Dext Bandwidth: n.a.

Quantif. Method: Pigments Quant Dilution Factor: 1.0000

Recording Time: 29/08/2012 4:01 Sample Weight: 1.0000

Run Time (min): 30.00 Sample Amount: 1.0000

No. Ret.Time Peak Name Cal.Type Points Corr.Coeff. Offset Slope Curve

min %

1 3.23 Peri Lin 6 99.9523 0.0000 0.0106 0.0000 2 3.43 Chlc2 Lin 6 99.9709 0.0000 0.0271 0.0000 3 4.89 Fuco QOff 13 99.5971 -0.0937 0.0154 0.0000 5 6.83 Ddx Lin 6 99.9501 0.0000 0.0126 0.0000 8 7.65 Allo Lin 8 99.5207 0.0000 0.0216 0.0000 9 7.99 Zea Lin 9 99.8774 0.0000 0.0217 0.0000

10 8.25 Lut Lin 3 99.9058 0.0000 0.0249 0.0000

14 9.79 Cant Lin 6 99.9627 0.0000 0.0123 0.0000

20 11.01 Chlb Lin 6 99.9768 0.0000 0.0088 0.0000

22 12.62 Chla LOff 7 99.9582 0.0905 0.0151 0.0000

23 13.25 DVCh-a Quad 5 99.7899 0.0000 0.0098 0.0000

24 14.09 Echi Lin 8 99.8732 0.0000 0.0157 0.0000

27 19.87 Phta Lin 10 99.8384 0.0000 0.0016 0.0000

29 21.53 Acar Lin 6 99.9385 0.0000 0.0212 0.0000

Average: 99.8651 -0.0002 0.0156 0.0000

Calibration-Curves-Pigments_APR2013.xls/Calibration(Batch)

Chromeleon (c) Dionex 1996-2001

Version 6.50 SP1 Build 956


12/05/2014 11:00 AM




Pigments-Calibration Curves

Sample Name: Injection Volume: 350.0

Vial Number: Channel: UV_VIS_1

Sample Type: STDs Wavelength: n.a.

Control Program: PDA Pigments-3D Bandwidth: n.a.

Quantif. Method: Pigments Quant Dilution Factor: 1.0000

Recording Time: 22/07/2013 18:50 Sample Weight: 1.0000

Run Time (min): 24.00 Sample Amount: 1.0000

No. Ret.Time Peak Name Cal.Type Points Corr.Coeff. Offset Slope RSD,%

min %

1 3.25 Peri Lin 6 99.9472 0.0000 0.0106 2.7475

2 3.46 Chcl2 Lin 6 99.9472 0.0000 0.0281 2.2608

3 4.99 Fuco QOff 12 99.9472 -0.1164 0.0156 4.3170

4 6.89 Ddx Lin 6 99.9729 0.0000 0.0124 3.2959

5 7.62 Allo Lin 8 99.8206 0.0000 0.0203 6.5534

6 7.67 Myxo Lin 4 99.9979 0.0000 0.0116 1.5062

7 8.21 Lut Lin 3 99.8108 0.0000 0.0265 5.0230

8 8.21 Zea Lin 9 99.9015 0.0000 0.0214 5.0277

9 9.76 Cant Lin 6 99.9624 0.0000 0.0125 3.3784

10 10.88 Chlb Lin 6 99.9758 0.0000 0.0088 2.6494

11 12.63 Chla LOff 13 99.9815 0.0393 0.0166 4.6647

12 13.45 DVCh-a Quad 5 99.8103 0.0000 0.0098 1.2773

13 13.87 Echi Lin 8 99.8882 0.0000 0.0156 5.3310

14 19.88 Phta Lin 10 99.7973 0.0000 0.0096 7.6010

15 21.53 Acar Lin 6 99.9332 0.0000 0.0213 6.8032

16 21.81 Beta Lin 7 99.7508 0.0000 0.0193 10.6720

Average: 99.8965 -0.0055 0.0158 4.8643

Pigments-Calibration-Curves-2013.xls Page 1

Appendix 4 HPLC Calibration Curves - Roberts Bank … 4 HPLC Calibration Curves This Page Left...

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