ApoTome2 Quick Guide.pdf
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Transcript of ApoTome2 Quick Guide.pdf
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Ap
oTo
me
.2 o
n A
xio
Im
ag
er.
Z1
Qu
ick
Op
era
tin
g G
uid
e
1.
Tu
rn o
n M
ain
Sw
itch
( R
ed
la
be
led
A &
B)
2.
Tu
rn o
n a
ll p
ow
er
sup
pli
es
(Sti
cke
r N
o.
1),
wa
it u
nti
l T
FT
fu
lly
lo
ad
3.
Tu
rn o
n H
BO
po
we
r b
ox
(Sti
cke
r N
o.
2),
sw
itch
on
Ap
oT
om
e.2
co
ntr
ol B
ox
(Sti
cke
r N
o.
3)
an
d
Tu
rn o
n t
he
PC
(S
tick
er
NO
.4).
Se
lect
Z
eis
s
use
r
4.
Pre
ss L
oa
d P
osi
tio
n o
n T
FT
to
lo
we
r th
e
ob
ject
ive
s
5.
Pla
ce s
pe
cim
en
sli
de
, se
cure
wit
h s
lid
e h
old
er
8.
Pre
ss
on
TF
T a
ga
in f
or
ob
ject
ive
ba
ck t
o
wo
rkin
g p
osi
tio
n
Sw
itch
ing
On
th
e S
yst
em
& W
ork
ing
wit
h S
pe
cim
en
Notes:
P
ut
on
a d
rop
of
imm
ers
ion
oil
on
to
p l
en
s o
f
63
x a
nd
10
0x
oil
ob
ject
ive
be
fore
use
Mic
rosc
op
e O
pe
rati
on
1.
Do
ub
le c
lick
on
ZE
N 2
01
1 s
oft
wa
re ic
on
on
de
skto
p
to s
tart
th
e s
oft
wa
re
2.
In Z
en
20
11
so
ftw
are
, g
o t
o L
oca
teta
b o
n l
eft
to
ol
are
a t
o e
xam
ine
th
e s
pe
cim
en
on
mic
rosc
op
e a
nd
ide
nti
fy r
eg
ion
of
inte
rest
3.
Pre
ss A
cqu
isit
ion
bu
tto
n t
o c
on
tro
l th
e m
icro
sco
pe
4.
Un
de
r S
ma
rt S
etu
p,
sele
ct r
esp
ect
ive
flu
ore
sce
nt
use
d f
or
the
sa
mp
le w
ith
th
e f
lexi
bil
ity
of
cha
ng
ing
the
co
lor
acc
ord
ing
to
pre
fere
nce
12
3 4
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
1
-
75.
Go
to
Li
ve
to
pre
vie
w t
he
sa
mp
le (
focu
s o
n y
ou
r sa
mp
le a
nd
do
ne
cess
ary
ad
just
me
nt
to a
chie
ve
be
st i
ma
ge
)
6.
Ad
just
E
xp
osu
re t
ime
fo
r e
ve
ry s
ing
le c
ha
nn
el(
dy
e)
7.
Fo
r C
on
ve
nti
on
al
Ima
gin
g,
Cli
ck
Sn
ap
to
ca
ptu
re a
2D
Im
ag
e
8.
Fo
r O
pti
cal
Se
ctio
nin
g w
ith
Ap
oT
om
e,
mo
ve
Ap
oT
om
e s
lid
er
in m
an
ua
lly
an
d s
et
op
tim
um
pa
ram
ete
r fo
r im
ag
ing
cap
turi
ng
u
nd
er
Ap
oT
om
e
Ta
b b
efo
re g
o t
o L
ive
to
pre
vie
w a
nd
pro
cee
d w
ith
exp
osu
re t
ime
ad
just
me
nt
an
d
Sn
ap
foll
ow
ed
by
Min
/ M
ax
for
ev
ery
sin
gle
Ch
an
ne
l
No
tes:
R
em
em
be
r to
Mo
ve t
he
sli
de
r o
ut
to v
iew
im
ag
e in
soft
wa
re,
rem
ind
er
me
ssa
ge
s a
s b
elo
w,
Cli
ck O
K
Le
ave
Ap
oT
om
e s
lid
er
OU
T f
or
Co
nve
nti
on
al
Flu
ore
sce
nce
Im
ag
ing
(1)
an
d g
en
tly
mo
ve i
n A
po
To
me
Sli
de
r m
an
ua
lly
fo
r O
pti
cal
Se
ctio
nin
g (
2)
F
or
op
tim
um
im
ag
e q
ua
lity
, re
me
mb
er
to c
lick
Min
/
Ma
x u
po
n S
na
p
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
2
-
56
7
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
3
-
8Ap
oTo
me
Se
ttin
gs
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
4
-
3D
Im
ag
ing
Acq
uis
itio
n (
Z-S
tack
Mo
du
le)
1.
Ch
eck
on
ch
eck
bo
x o
f Z
-sta
ck e
xpe
rim
en
t. T
he
Z-S
tack
To
ol b
ar
ap
pe
ars
.
2.
Cli
ck o
n L
ive
bu
tto
n t
o f
ast
sca
n t
he
sp
eci
me
n.
3.
Usi
ng
fo
cus
dri
ve
of
the
mic
rosc
op
e t
o f
ocu
s o
n t
he
hig
he
st p
osi
tio
n o
f th
e s
pe
cim
en
. C
lick
on
S
et
Fir
st
bu
tto
n t
o s
et
the
hig
he
st
po
siti
on
of
the
Z-s
tack
. F
ocu
s in
dif
fere
nt
dir
ect
ion
to
th
e l
ow
est
po
siti
on
of
spe
cim
en
. C
lick
on
th
e
Se
t La
st
bu
tto
n t
o s
et
the
low
est
po
siti
on
. C
lick
ag
ain
on
Liv
e b
utt
on
to
sto
p s
pe
cim
en
sca
nn
ing
(p
rev
en
t sp
eci
me
n f
rom
ble
ach
ing
).
4.
Cli
ck o
n t
he
nu
mb
er
of
slic
e t
hic
kn
ess
aft
er
Op
tim
al
to o
pti
miz
e n
um
be
r o
f sl
ice
s to
ma
tch
th
e o
pti
ma
l Z
-in
terv
al
to t
he
sta
ck
size
. U
nd
er
Op
tim
ize
Se
ctio
nin
g a
nd
ste
pse
ctio
n,
cho
ose
Op
tim
al
bu
tto
n t
o m
atc
h t
he
op
tica
l se
ctio
n f
rom
ea
ch c
ha
nn
el.
5.
Cli
ck o
n S
tart
Ex
pe
rim
en
t b
utt
on
to
sta
rt r
eco
rdin
g o
f th
e Z
-Sta
ck.
6.
Cli
ck
Ga
lle
ry
to v
iew
all
th
e s
lice
s ca
ptu
re w
ith
S
ho
w D
ime
nsi
on
La
be
ls
for
thic
kn
ess
de
tail
s
1
2
3 3
4
4
5
2
1
2
33
4
5
6
6
5
-
3D
Pro
ject
ion
7
1.
Fo
r 3
D P
roje
ctio
n,
cli
ck 3
D o
n t
he
le
ft p
an
el
of
the
Im
ag
e w
ith
va
rio
us
op
tio
n f
or
3D
vie
w
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
6
-
To S
av
e I
ma
ge
s
1.
Go
to
Fil
e,
Sa
ve
or
Sa
ve
as,
se
lect
th
e f
old
er
an
d S
av
e t
yp
e
2.
Fo
r 3
D I
ma
ge
s, g
o t
o f
ile
an
d s
ele
ct e
xpo
rt o
pti
on
Fo
r fu
rth
er
de
tail
s, k
ind
ly c
on
tact
r.r
am
up
illa
i@ze
iss.
com
7