AnviRIV 1, 2, 3: ELISA Kits for potato...

CATALOGUE OF PROMISING HORTICULTURE TECHNOLOGIES 88

PRODUCTS AnviRIV 1, 2, 3:

ELISA Kits for potato virus

Utilization:- For fast detection of several virus infections on potato such as Potato

Leaf-Roll Virus (PLRV), Potato Virus Y (PVY), Potato Virus X (PVX) and Potato Virus S (PVS)

- Each polyclonal antiserum produced by pure virus injection in rabbits- Antiserum used to detect virus with enzyme-linked immunosorbent assay

(ELISA) Test Superiority:

- Faster detection results compared to other methods- Needed high sample number for testing compared to bioassay test using

indicator plant and other methods- Sensitiveandspecific

Benefit:- Usedforvirusdetectiononseedandfieldsample

Potency: - Cheaper compared to imported test kits

Status: offered for commercialization

89 CATALOGUE OF PROMISING HORTICULTURE TECHNOLOGIES

Active ingredient: Phthorimaea oprculella Granulosis virusFormulation: powder having stomach poison.Utility: - To control Phthorimaea oprculella pest

on potatoSuperiority:- Select ive, only for Phthorimaea

oprculella- Persistent to plant and soil- Safety for human and environment- Easily combined with other controlling

methods

BiaRIV-1

- Cheaper compared to synthetic insecticide

Benefit:- Effective to kill Phthorimaea oprculella

involved on the resistant pest due to insecticide application

- Application for tuber: 125 g/25 kg tuber

Potency:- Reduce application of synthetic

insecticide- Reduce environmental pollution



BiaRIV-2

Bionok

• Inhibit pest reproduction rate up to74%

• Toxin isproteinthatcanbemultipliedand made synthetically

• 80%offarmercanaccept• Utilizedforbiodiversityconservation• Reducesyntheticpesticidethatgivehigh

effect on antagonism microbes Status: offered for commercialization

Activeingredient:Spodopteraexiguapolyhydrosis virusFormulation: powder having stomach poisonUtility: - To control Spodoptera exigua pest on

shallot or onionSuperiority:- Selective,onlyforSpodopteraexigua- Persistent to plant and soil- Safety for human and environment- Easily combined with other controlling

methods- Cheaper compared to synthet ic

insecticide Benefit:- Effective to kill Spodoptera exigua


- Applicationinthefield:600g/haPotency:- Reduce appl icat ion of synthet ic

insecticide- Reduce environmental pollutionStatus: offered for commercialization


BiaRIV-3

Active ingredient: Cucumber mosaic virus associated with RNA-5 (CARNA-5)Utility:

To control cucumber mosaic virus (CMV) on vegetablesSuperiority:

•Canbeappliedforvaccineonplantnursery.PlantnurserythatwasvaccinedcankeepsurviveondangerousCMVattackinthefield

•SafetyforenvironmentandantagonismwithotherimportantvirusesonvegetablesBenefit:

•ReduceplantdamageduetoCMVattackPotency:

•KeepandincreaseplantproductivityStatus: offered for commercialization


Active ingredient: Tephrosia vogelii (75%) and Lantana camara (25%)Formulation: powder and act as repellent Utility: - To control Phthorimaea oprculella pest

on potato tuber in storageSuperiority:- Select ive, only for Phthorimaea

oprculella- Persistent to plant and soil- Safety for human and environment- Easily combined with other controlling

methods- Cheaper compared to synthet ic

insecticide Benefit:- Effective to kill Phthorimaea oprculella


- Application for tuber: 100 g/10 kgtuber

Potency:- Reduce appl icat ion of synthet ic


BIOTEP/TEPTANG


Activeingredient:PseudomonasfluorescensStrainMR56Utility: - To control Fusarium wilt, Phytium,

Rhizoctonia sp. and Alternaria sp.Superiority:- Did not leave remains of poison for

plant- Safety for environment- CheapandeasytofindApplication:- One day before planting or in seed

planting or dipping seedling in BIO-PF solution(10mlBIO-PF/literofwater)

BIO-PF

- Two weeks after planting by spreading of the BIO-PF in the same concentration

Benefit:- Effective to reduce soil-borne diseasesPotency:- Reduce appl icat ion of synthet ic



BIOTRI

Biotri is biofungicide with Tricoderma harzianum as active ingredient that was produced by Biocontrol Laboratory of Indonesian Ornamental Crops Research In-stitute. The biofungicide was consisted of organic manure, plant compost that was decomposited by T. harzianum.Superiority: - Control soil-borne diseases such as Fusarium wilt, bacterial wilt (Ralstonia

solanacearum), damping off (Phytium sp), stem rot (Rhizoctonia solani) on ornamental plants, vegetables and fruits

- Easy in application- Safety for plant and environment- Cheapandeasilyfind- Did not leave residue on plantStatus: offered for commercialization


Gliostar

Gliostar is a starter material to control Fusarium wilt with Gliocladium sp. as active ingredient. The fungi was isolated from chili rhizosphere in farmer land in Sukabumi-West Java.Application:- Weighted250gGliostarmixedwith25kgraworganicmanure.- Mixtillhomogen- Leavethemixturefor10-15daysinopenarea- Dailymixingcarriedouttomakethematerialdecomposedbetter- TocontrolFusariumonbanana,mixthewelldecomposed-organicmanurebyGliocladium

sp with soil in ratio 1:1 (v/v)- Apply this composition to cover banana planting hole - Secondapplicationwillbedone3-6monthsafterplantingbyspreadingorganicmanure

infestedbyGliocladiumsparoundbananaplantwith concentration0,5kgorganicmanure/plant



Gliocompost

Active ingredient: Gliocladium spFormulation: Raw organic manure infested by Gliocladium spUtility: - To control Fusarium wilt, Phomopsis

sclerotioides, Phytium, Rhizoctonia solani and Sclerotinia sclerotiorum on ornamental plants, vegetables and fruits

Superiority:- Did not leave remains of poison for

plant- Safety for environment- CheapandeasytofindApplication:- 10ggliocompost/plantingholeor0,5kg

gliocompostcanbemixedwithnurserymedia for 2 m2

- Forpottingmedia,1:5-10(v/v)betweengliocompost and soil

Benefit:- Effective to reduce soil-borne diseasesPotency:- Reduce appl icat ion of synthet ic



Mikoriza

BIORIZA01K,BIORIZA02K,andBIORIZA02Gare biofertilizer processed biotechnologically to support agriculture environmental friendly andefficientBenefit:- Increase nutrient uptake- Increaseefficiencyonsyntheticfertilizers

use- Increase plant growth and its rooting

system- Reduceplantstressduetowaterdeficiency

and root disease attack - Once application give benefit in long

period in plantDosage:- One capsule of BIORIZA 01 K ANDBIORIZA02Kforoneseedlingbydippingit5-10cmindepthingrowthmedia

- BIORIZA02Gsuitableforseedlingnurseryby preparing media (both in nursery bedandbox). Fumigation is suggestedbeforeBIORIZA02Gapplication.SpreadBIORIZA02G(±50g/m2),andcoveredwithnurserymedia(±1cminthickness)twice.



Prima BAPF

Active ingredient: Bacillus sp and Pseudomonas fluorescensUtility: - To control Fusarium wilt, bacterial wilt

(Ralstonia solanacearum), club-root (Plasmodiophora brassicae), dumping off (Phytium sp), Rhizoctonia solani and white-rush (Puccinia horiana) on ornamental plants, vegetables and fruits

Superiority:- Did not leave remains of poison for

plant- Safety for environment- CheapandeasytofindApplication:- Before seed sowing, soaking seed/seedling

for30mininthe0,5%biofungicide(5ml/liter of water) and then spraying on the nursery media

- Before planting, spraying planting hole with the Prima BAPF with the same concentration

- Repeated the application when the plant is in 15 days after planting and applied in 7 days interval for rooting area.

- For leaf soft-rot and white rush, spraying plant leaves with Prima BAPF, 7 days interval in the same concentration is suggested

Benefit:- Effective to reduce soil and air-borne

diseasesPotency:- Reduce appl icat ion of synthet ic

insecticide- Reduce environmental damageStatus: Have been collaborated with PT. Primasid Andalan Utama, Jl. Berdikari I/A BlokI/190Jakarta13340


M-RIF is a product prototype for controlling Fusarium wilt resulted by Indonesian Tropical FruitResearchInstitute.TheproductwasinspiredbyS-Hmixture(S-Hisabbreviationfromformulator names i.e. Sun and Hwang from Taiwan). M-RIFisbiologicalcontrolagentwithbasicmaterialscomefrommixingricestraws,burned-ricehusk,urea,agriculturelime,NPK,remainofbodyfish,ZnSO4andCuSO4.M-RIFmediacan be applied individual or in combination to biological control agent. Applicationwascarriedoutbygiving1-2%mediaM-RIFinplantingholes,mixingwiththesoil and placed back in the planting holes.M-RIF composition:- Ricestraw :67kg- Burned-ricehusk :20kg- Urea :8kg- Agriculture lime : 4 kg- NPK(15:15:15) :1kg- Remainsofbodyfish:200g- ZnSO4(Technical) :10g- CuSO4(Technical) :10gStatus: offered for commercialization

M-RIF


Citrus essential oil was produced by extractingcitrus fruitskinusingdistillationsystem. Although there are so many citrus varieties, it is not all citrus varieties can be exploredtheiressentialoil.Mainsignalusedas indicator that citrus variety has potential itsessentialoilistheexistenceoflimonenecompound in the variety. Generally limonene compound is dominant compound in citrus essential oil. Variation of citrus essential oil aroma in several citrus varieties is de-termined by other content and compound compositions of non-limonene compound.

Essential oil extraction procedure ofcitrus fruit skin is:- Cutting citrus fruit skin into small pieces

(±2mm2square)tomakeeasyreleasingprocess of essential oil. To optimize in obtaining optimal volume of essential oil, drying citrus fruit skin in open area for 3-4 days is suggested.

- Blending the citrus fruit skin pieces till produce fruit skin pulp

- Extracting the pulp using distillationsystem, where water vapor from boiled-water was used to release essential

Citrus Essential Oil and its Benefit

oil from citrus fruit skin cells. Time consumingfortheextractionwasusually2-3 h.

- Receive essential oil resulted from distillation process in different container with splitting funnel

- Separating essential oil from water using pipette and placed in essential oil bottles

Citrus essential oil was usually clear. Topreventessentialoildamageandextendessential oil storability, it was suggested to place the oil in dark bottles and saved in refrigerator.

Citrus essential oil can be used as - Making room fragrance, perfume, and

altering food performance. - Therapy aroma for healthy purposes- Stabilizing nerve system, feel better and

comfortable - Increasing eat appetite- Disease healing

Several citrus essential oil components with their role that are important to support


human health are:- Limonene

• Toaccelerateblooddistribution• Tohealbronchitisandcouch• Toinhibitcancercellsgrowthsuchas

gastric, lung and skin cancer- Linalool

• Sedativeeffect- Acetic linalil

• Sedativeeffect- Therpinol

• Sedativeeffect- Citronella

• Sedat ive effect and mosquitorepellent

Several citrus essential oils and their benefitondiseasehealingare:- Sweet citrus (Citrus sinensis): sedative,

tonic, hypnotic, antiseptic- Lime citrus (Citrus hystrix): sedative,

mosquito repellent, tonic, and coldreducing

- Grape fruit: cancer cell inhibitor due to high limonene content

- Lemon citrus (Citrus lemon): anti-hypertension, tonic and anti-bacterial

Several application methods of es-sential oil:

- Using traditional cook stove: 3-4 ml warm water on the traditional cook stove was added 2-4 drops of essential oil. Warm water will cause essential oil evaporate tofulfillallroomswithcitrusaroma

- Warm water used to take a bath was given 4-6 drops essential oil, so inbetween taking a bath citrus aroma will be inhaled and get in the body via skin.

- Inhalation: Plastic container with hot water was added with 2-3 drops of essential oil. To have benefit citrus essential oil, covered head with towel and positioned closer to plastic container and inhale evaporate citrus aroma. Towel will help to maintain high loss citrus aroma.

- Dropping essential oil on tissue paper was used to make fragrance of telephone handle or direct inhaled

- Using formassagepurposesbymixingtheessentialoil(10drops)withmassageoil(30ml)suchaswijenoil,soybeanoil,zaitunoil,sunfloweroil.

- Mixingessentialoilwithotheressentialoils such as from bergamot, cedar wood, geranium, ginger, lavender, rosemary, lemon grass, lantana camara, cendana wood, and pathcouli can be used to heal several diseases.

Status: ready used for training and extensionpurpose


•CVPDvectorcontrol(Diaphorinacitri)•Appliedinrainyseason•Target:adultpest•Applicationmethod:spotreleaseStatus: offered for commercialization

Taracid Biological Product

•CVPDvectorcontrol(Diaphorinacitri)•Appliedindryseason•Target:instarnymph3-5•Applicationmethod:spotreleaseStatus: offered for commercialization

Latricid Biological Product

GLIOCID

• Active ingredient: Gliocladium spp 108with corn media

• Dosage:10g/lwater• Utility:tocontrolSpacelomafawcetiStatus: offered for commercialization


Active ingredient: Azadiractine Formulation:mimbaseedextractUtility: to control thrip Eriophydae on citrus, Aonidiella aurantii, Diaphorina citriSuperiority:

• Safetyforhumanandenvironment• Can be combined with other

methods• Safety for biological control agent

population of citrus• Cheaperthansyntheticinsecticide


Mimba seed extract product

Eugenia aromatic extract

Active ingredient: eugenol and phenol compoundUtility: to control basal stem and root rot diseaseSuperiority:

• S a f e t y f o r h u m a n a n denvironment

• Can be combined with othercontrolling methods

• Ready solution was obtained bydiluting stock solution (±800 cc)in 4 liter water

Preparingmethod:100gEugeniaaromaticpowder in a liter water then fermented for one night and filtering the stocksolution

Application: the extract was applied bydrenching in rooting area and painting ondamagebasalstem(200cc/ph)



- To control soil-borne diseases such as Fusarium sp, Phytophthora sp, Verticilium sp

- Compost decomposer- Application dosage:

•Sowed the triocid around plantrootingareaandmixwellwithsoil

•Mixed triocid during compostmaking


Sentor

• Organicfertilizer

• Acceleraterootgrowthsonutritionuptakefrom the soil increased such as 5% N, 5% P2O5,5%K2O,3,800ppmCa,1,600ppmMgO,1,5%S,4,000ppmMn,3,000ppmFe,3,000ppmZn,750ppmB,25ppmMo.

• Speed up shoot cel l divis ion andflowering

• Reduceflowerandfruitfallness

• Increasefruit/tubersize,sugarcontentinfruitjuiciness,seedquality

• Prevent nutrient deficiency especially inmacro nutrients

• Increase physiological stress due toclimate change and pest-disease attack


TRICHOCID


- Repellent was originated from essential oil extractedfromCymbopogonnardusandmixedwithliquidparaffin

- Liquid paraffin was carriermaterial ofessential oil that prevent faster essential oil evaporation

- Superiority:• Environmental friendly due to

using natural resources availability surround us

• Have high effect to control andreduce fruit borer pest on citrus up to70%

Status:readyusedfortrainingandextensionpurposes

Repellent formulation for Fruit Borer Pest (Citripestis sagittiferella) on citrus

• Trapmaterialwasoriginatedfromessentialoilextracted fromEupatoriumunifoliumthatwasmixedwithliquidparaffin.

• Environmental friendly due to usingnatural resources around user or farmer

• Reduce dependence on synthetic trapthatwasexpensiveanddifficulttofinditandonlysoldbyspecificcompanylocatedabroad.

Status:readyusedfortrainingandextensionpurposes

Bactocera tau trap formulation

AnviRIV 1, 2, 3: ELISA Kits for potato...

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