ANTIMICROBIAL AND ANALGESIC ACTIVITIES OF DELONIX …ayushportal.nic.in/pdf/12690.pdf · 2012. 3....
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Aryavaidyan Yol. XYI., No.1, Aug. - Oct. 2002, Pages 51 - 53 ANTIMICROBIAL AND ANALGESIC ACTIVITIES OF DELONIX ELATA GAMBLE AND DELONIX REGIA RAF. YN. Seetharam, Vijay, G. Sharanabasappa, N. Srinivas Murthy and VR. Sangamma* Abstract: The ethanolic extract of Delonix elata Gamble and Delonix regia Raf. have shown good antimicrobial actvity (40 mg/IO ml) and analgesic activity at the dose of 200 mg/kg body weight. Introduction Plants playa vital role in curing various ail- ments of man. Being in association with plants for the long time, he started using them as food, fuel etc. At the same time, he used plants for his own diseases and health. So, in the recent past, he used medicinal plants in vari- ous combinations or single plant as a drug to cure problems of central nervous system. Only during 20 th Century, scientists started screen- ing the natural products for various pharmaco- logical activities like antimicrobial activity and analgesic activity etc. The plants under study Delonix elata Gamble and Delonix regia Raf are very good medicinal plants. Delonix elata Gamble is used in rheumatism and flatulence.'. Delonix regia Raf is known to contain lectins in it. 2. Delonix elata Gamble has shown very good anti in- flammatory activity, it is also known that it contains biflavanoids like rutin, quercitin, 3-0- galactoside, which were isolated from water extract', therefore here is an attempt to screen these plants for their antimicrobial and analgesic activities. Materials and methods Delonix elata Gamble and Delonix regia Raf the important medicinal plants were collected from Gulbarga University campus and Santraswadi Gulbarga. The plant species collected were identified with the help of "Flora of the Presidency of Madras":' by Gamble J.S. (1935) and these specimens were deposited in Herbarium Department of Botonay GUG (HGUG) Healthy Delonix elata Gamble and Delonix regia Raf plants leaves shade dried, powdered and extracted successfully with ethanol (95%). The antimicrobial activity was carried out against four bacteria viz. E. coli. S. aureus, P. vulgaris, Pseudomonas aeruginosa and a fun- gus Aspergillus niger using cup plate method", The analgesic activity was carried out by tail flick method". Totally 20 mice were taken for the study and were grouped in four categories of 5 animal each. The analgin was used as a standard drug. "Bio-systematics and Medicinal Plants Laboratory, Department of Botany, Gulbarga University, Gulbarga, Karnataka. aryavaidyan 51
Transcript of ANTIMICROBIAL AND ANALGESIC ACTIVITIES OF DELONIX …ayushportal.nic.in/pdf/12690.pdf · 2012. 3....
Aryavaidyan Yol. XYI., No.1, Aug. - Oct. 2002, Pages 51 - 53
ANTIMICROBIAL AND ANALGESIC ACTIVITIES OFDELONIX ELATA GAMBLE AND DELONIX REGIA RAF.
YN. Seetharam, Vijay, G. Sharanabasappa, N. Srinivas Murthy and VR. Sangamma*
Abstract: The ethanolic extract of Delonix elata Gamble and Delonix regia Raf.have shown good antimicrobial actvity (40 mg/IO ml) and analgesic activity at thedose of 200 mg/kg body weight.
Introduction
Plants playa vital role in curing various ail-ments of man. Being in association with plantsfor the long time, he started using them asfood, fuel etc. At the same time, he used plantsfor his own diseases and health. So, in therecent past, he used medicinal plants in vari-ous combinations or single plant as a drug tocure problems of central nervous system. Onlyduring 20th Century, scientists started screen-ing the natural products for various pharmaco-logical activities like antimicrobial activity andanalgesic activity etc.
The plants under study Delonix elata GambleandDelonix regia Raf are very good medicinalplants. Delonix elata Gamble is used inrheumatism and flatulence.'. Delonix regia Rafis known to contain lectins in it. 2. Delonixelata Gamble has shown very good anti in-flammatory activity, it is also known that itcontains biflavanoids like rutin, quercitin, 3-0-galactoside, which were isolated from waterextract', therefore here is an attempt to screen
these plants for their antimicrobial andanalgesic activities.Materials and methodsDelonix elata Gamble and Delonix regia Rafthe important medicinal plants were collectedfrom Gulbarga University campus andSantraswadi Gulbarga. The plant speciescollected were identified with the help of "Floraof the Presidency of Madras":' by Gamble J.S.(1935) and these specimens were deposited inHerbarium Department of Botonay GUG(HGUG)Healthy Delonix elata Gamble and Delonixregia Raf plants leaves shade dried, powderedand extracted successfully with ethanol (95%).The antimicrobial activity was carried outagainst four bacteria viz. E. coli. S. aureus, P.vulgaris, Pseudomonas aeruginosa and a fun-gus Aspergillus niger using cup plate method",The analgesic activity was carried out by tailflick method". Totally 20 mice were taken forthe study and were grouped in four categoriesof 5 animal each. The analgin was used as astandard drug.
"Bio-systematics and Medicinal Plants Laboratory, Department of Botany, Gulbarga University,Gulbarga, Karnataka.
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ObservationsIn the present study of plants screened fortheir antimicrobial and analgesic activities. Theethanolic extract of Delonix elata and Delonixregia Raf. plant showed mild to marked antimi-crobial activity against all the tested mocrobes(Bacteria and fungi Viz. E. coli, S. aureus, P.vulgaris, Pseudomonas aurginosa and a fun-gus Aspergillus niger). The results have beencompared with standard drugs streptomycinand nystain.
The antibacterial activity of ethanolic leaf ex-tract of Delonix regia Raf. has showed inhibi-tory activity against all the bacteria tested.Whereas the leaf extract of Delonix elataGamble has shown moderate antibacterial
activity against S. aureus amd P. vulgaris whencompared to the activity of streptomycin (stand-ard). The antifungal activity of ethonolic ex-tract of Delonix elata Gamble showed mildinhibitory effect on the fungus A. Niger whereas the plant extract of Delonix regia Raf. hasshown very good inhibitory effect on A. nigeras compared to the nystatin (standard), thezone of inhibition is more than that of standardnystatin (Table-I).
The analgesic activity results indicate thatethonolic extract of Delonix elata Gamble andDelonix regia Raf at a concentration 200mg/kgbodyweight have shown good analgesic activ-ity after 60 minute of drug administration whencompared to the standard anal gin (Table 2).
Antimicrobial activity of ethanolic extract of Delonix elata & Delonix regia
TABLE - 1
DOSAGE DIAMETEER OF INHIBITION ZONE (IN MM)Microbes
Mg/lOml D. elata D. regia Stretomycin Nystatin
E. coli 40 IS.5 ± O.S 14.0± 1.0 19.0± 0.0P.aeruginosa 40 14.0 ± 1.0 14.S± O.S 23.0± 0.0S.aureus 40 14.S ± O.S 13.6± O.S 17.0± 0.0P.vulgaris 40 17.S ± O.S lS.S± O.S 19.0± 0.0A.niger 40 2S.S ± O.S 34.S± O.S 33.0 ±O.O
Value = Mean ± S.E., Well Size 8.0 mm
TABLE - 2
Analgesic activity of Delonix elata & Delonix regia
Test sampleDOSE TIME IN SECONDS
mg/kg(oral) 30min 60rnin 90min 120min
D. elata 200 I.S ± O.S 3.7S ± 0.249 6.5 ± O.SO 3.25± 0.249D. regia 200 1.0 ± 0.0 7.S0 ± 0.749 6.2S± 0.249 3.S ± 0.S02Standard (Analgin) 200 I.S ± O.S77 10.1 ± 0.0997 10.0 ± O.S77 10.0 ± O.S77Control Sml 1.0 ± 0.0 1.0 ±O.O 1.0 ±O.O 1.0 ± 0.0(1%Gum acacia)
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ConclusionThe antimicro.bia! and analgesic activity of D.elata Gamble and D. regia Raf may be due tothe presence of the secondary metobolites likeflavonoids? and alkaloids" .Further, a detailed study on these plantsextracts, isolation of compounds which areresponsible for their antimocrobial andanalgesic activities from these plants and theirstructural elucidation are need of the situation.Acknowledgement:The authors are thankful to Dr.. BV fatil, M.R.Medical college, Gulbarga for providingexperimental animals.
References:I. Kiritikar, K.R. and Basu, B.D., Vol II, 2nd
Ed., Lalit Mohan Basu Alahabad, India, 1980
Asianel- Every Sunday 8.50 - 9.00 in Ihe morning
2. Sammour, R.H. and EL-Shanshoury, A.E.R.R., Botanical Bulletin of Academic Sinica33(20): p.185-190, 1992.
3. Sethuraman M.G., Sulochana N., CurrentScience: p.55, 7, 343, 1986.
4. Gamble, J.S., Adlard and Son's Ltd., w.e.London, Vol I.,p.273-435, 1935.
5. Indian Pharmacopoeia, Appendix IV, 90,3rd Ed., Government of India, New Delhi, 1985.
6. Kulkarni, S.K., 1" Ed., Vallaha PrakashanPublishers, Delhi
7. Raj,K., Jaggi and Shalini Kapoor, IndianDrugs, 369(11), p.668-678, 1999.
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ayurarogyasaukhyamTHE KOTTAKKAL INITIATIVE
Arya Vaidya Sala has started an attractive TV programme to inform the public aboutayurvedic remedies for important ailments. The programme in thirty episodes of tenminutes each covers the management of heart diseases, diabetes, blood pressure, cancer,occupationalhazards and various other ailments. Specialtopicson gynaecand paediatrics,geriatrics, etc. are also included.Each capsule is prepared by a team of doctors of Arya Vaidya Sala. Phone calls,e-mails and letters we receive show a massive response to ayurvedic health-care. Thetext of the episodes is being published for the benefit of the public.
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