Antibody Product list - ProSci Incorporated · Product Name Cat. No. Species Application...

Antibody Product list

13100 Kirkham Way Tel: 858-513-2638Suite 207 1-888-513-9525Poway, CA 92064 Fax: 858-513-2692www.prosci-inc.com [email protected]

Product Name Cat. No. Species Application Description Release Date

Apoptosis-Chromatin condensation and DNA fragmentation factors

Acinus (CP) 2215 H WB Inducer of chromatin condensation 4thQ 2000

Acinus (CT) 2217 H WB Inducer of chromatin condensation 4thQ 2000

Acinus (IN) 2219 H WB Inducer of chromatin condensation 4thQ 2000

Acinus (NP) 2241 H WB Inducer of chromatin condensation 4thQ 2000

AIF (CT) 2301 H M R WB Apoptosis Inducing Factor 4thQ 2000

AIF (IN) 2267 H M R WB Apoptosis Inducing Factor

AIF (NT) 2239 H WB Apoptosis Inducing FactorCAD / DFF40 (CT) 2011 M WB Caspase Activated DNaseCAD / DFF40 (I17) 2007 M R WB Caspase Activated DNase

CIDE-A (CT) 2085 H WB Cell death-Inducing DFF-like Effector

CIDE-A (CT) 2089 M WB Cell death-Inducing DFF-like Effector 3rdQ 2000

CIDE-B (CT) 2091 M WB Cell death-Inducing DFF-like Effector DFF40 / CAD (I18) 2107 H M R WB Caspase Activated DNaseDFF40 / CAD (IN) 2155 H WB Caspase Activated DNaseDFF40 / CAD (NT) 2153 H WB Caspase Activated DNaseDFF45 / ICAD (CT) 1141 H WB DNA fragmentation factor & Inhibitor of CADDFF45 / ICAD (NT) 1148 H WB, IHC DNA fragmentation factor & Inhibitor of CADDNase II (CT) 2059 H WB DNase involved in apoptosis

EndoG 3035 H M R WB Endonuclease G New

ICAD / DFF45 (CT) 2003 M WB, IHC Inhibitor of CADICAD / DFF45 (NT) 2001 M WB, IHC Inhibitor of CAD

Apoptosis-Caspases

Casp-9 / Mch6 / Apaf-3 (IN1) 2071 H WB A new caspaseCasp-9 / Mch6 / Apaf-3 (IN2) 2073 H WB A new caspaseCasp-10 / FLICE2 (CT) 1128 H M R WB A new caspase

Casp-12 (IN) 2327 H M R WB, IHC A new caspase 4thQ 2000

Casp-12 (NT) 2325 H M R WB A casp. in Apoptosis & Alzheimer's Disease 4thQ 2000Casp-13 / ERICE 2245 H M R WB Evolutionarily Related ICE

Apoptosis-CED4 homologue

Apaf-1 (CT) 2015 H M R WB Apoptosis Protease Activating Factor

Apaf-1 (NT) 2013 H M R WB Apoptosis Protease Activating Factor FLASH (CT) 2271 H WB Homolog of CED4 4thQ 2000

Apoptosis-BcI-2 family

Bcl10 / CIPER / CLAP (NT) 2161 H WB, IHC Involved in apoptosis and tumor 4thQ 2000Bim / BOD (IN) 2065 H M R WB A new member in Bcl-2 family

Bmf (CT) 3029 H WB A new member in Bcl-2 family New

Bmf (NT) 3031 H M WB A new member in Bcl-2 family New

Bnip3L / Nix (IN) 2289 H WB A new member in Bcl2 family 4thQ 2000PUMA (CT) bbc3 3041 H M WB A novel member in Bcl-2 family NewPUMA (NT) bbc3 3043 H WB A novel member in Bcl-2 family New

Antibody Listed by Category


Apoptosis-TRAIL and TRAIL receptors

DcR1 / TRAIL-R3 (ED) 2179 H M R WB Decoy Receptor 1 for TRAILDcR1 / TRAIL-R3 (ED2) 2299 H M R WB Decoy Receptor 1 for TRAIL 3rdQ 2000DcR2 / TRAIL-R4 (ID) 2021 H M R WB Decoy Receptor 2 for TRAILDR4 / TRAIL-R1 (CT) 1139 H WB Death Receptor for TRAILDR4 / TRAIL-R1 (NT) 1167 H WB Death Receptor for TRAILDR5 / TRAIL-R2 2019 H M WB Death Receptor for TRAILTRAIL / Apo-2L (CT) 1113 H WB Ligand for DR4 and DR5

Apoptosis-Other cell death receptors

DR3 / Wsl-1 / Apo-3 (CT) 1120 H M R WB Cell Death ReceptorDR3 / Wsl-1 / Apo-3 (ED) 1158 H M WB Cell Death ReceptorDR3 / Wsl-1 / Apo-3 (NT) 1166 H M WB Cell Death ReceptorDR6 (NT) 2157 H WB Death Receptor 6

Apoptosis-TNF and Fas related molecules

ADAM10 / KUZ (CT) 2051 H WB TNFα converting enzyme

ARTS 3025 H WB An apoptosis related protein New

DcR3 / TR6 (NT) 2140 H M R WB Decoy Receptor for FASL and LIGHT

SODD (NT) 2143 H M R WB Silencer Of Death Domains TACE/adam17 (CT) 1131 H M R WB TNFα converting enzyme

Apoptosis-Regulating kinases

ASK1 / MAPKKK5 (CT) 1151 H WB Apoptosis Signal-regulation Kinase

DAPK2 2323 H M R WB Death-Associated Protein kinase 2 1stQ 2001

DRAK1 (NT) 2147 H WB DAPK Related Apoptosis-inducing Kinase

DRAK2 (CT) 2149 H WB DAPK Related Apoptosis-inducing Kinase RICK / RIP2 (CT) 2183 H WB RIP-like Interacting CLARP KinaseRICK / RIP2 (NT) 2075 H M R WB RIP-like Interacting CLARP Kinase

RIP3 2283 M R WB Mouse Receptor Interacting Protein 3 1stQ 2001ZIPK (IN) 2067 H M R WB Apoptosis inducing kinase

Apoptosis-Inhibitory molecules

ARC (CT) 2081 H WB Apoptosis Repressor with CARD domain

ARC (NT) 2185 H M R WB Apoptosis Repressor with CARD domainmFLIP / I-FLICE (CT) 1156 M WB FLICE inhibitory proteinFLIP / I-FLICE (CT) 1161 H M R WB FLICE inhibitory proteinFLIP / I-FLICE (NT) 1159 H M R WB FLICE inhibitory protein

FLIPs (CT) 2055 H WB FLICE inhibitory protein 3rdQ 2000

ILP-2 3017 H M R WB A novel member in IAP family New

Livin, KIAP 2505 H WB A novel member in IAP family New

Smac / DIABLO (CT) 2409 H M R WB IAPs binding protein 1stQ 2001mSmac / DIABLO (CT) 2411 H M R WB, IHC IAPs binding protein 1stQ 2001Survivin / TIAP (CT) 2235 H WB, IHC A new inhibitor of apoptosis 3rdQ 2000Survivin / TIAP (NT) 2233 H WB, IHC A new inhibitor of apoptosis 3rdQ 2000mSurvivin / TIAP (CT) 2237 M WB, IHC A new inhibitor of apoptosis 4thQ 2000


Apoptosis-Adapter molecules

ASC 2287 H WB A novel CARD-containing adaptor protein 1stQ 2001

CARD9 2515 H WB A new CARD-containing protein New

DEDAF 2227 H M R WB A novel regulator of apoptosis 3rdQ 2001

Daxx (CT) 1163 H M WB Adapter MoleculeF1Aα (CT) 2279 H M R WB A novel death receptor-binding protein 1stQ 2001RAIDD / CRADD (CT) 1117 H WB Adapter MoleculeRAIDD / CRADD (IN) 1115 H WB Adapter Molecule

Signal Transduction

CIKS/Act1 (CT) 2499 H WB A novel NF-κB activator 3rdQ 2001

CIKS/Act1 (NT) 2501 H WB A novel NF-κB activator 3rdQ 2001DOK (CT) 1107 H WB Tyrosine kinase substrate p62DOKIKKα / IKK-1 (C1) 2025 H WB, IHC IκB kinase alpha

IKKα / IKK-1 (C2) 2115 H WB IκB kinase alpha

IKKα / IKK-1 (C3) 2117 H WB IκB kinase alpha

IKKβ / IKK-2 (C3) 2121 H WB IκB kinase beta

IKKε / IKKi (CT) 2329 H WB IκB Kinase epsilon 1stQ 2001IKKγ / NEMO / FIP3 (NT) 2335 H M R WB IκB Kinase gamma 1stQ 2001

IL-1RAcP (C1) 2131 H WB IL-1R accessory protein

IL-1RAcP (C2) 2129 H WB IL-1R accessory protein

IRAK (CT) 1007 H M R WB, IP IL-1R associated kinase

IRAK-2 (C2) 2123 H WB IL-1R associated kinase

IRAK-2 (CT) 2213 H WB IL-1R Associated Kinase

IRAK-M 2355 H M R WB A novel IL-1R Associated Kinase 1stQ 2001MADD / DENN (CT) 1150 H M R WB Activator of MAPK pathway

MYD88 (CT) 2127 H M R WB Adapter in IL-1 & LPS signal

MYD88 (IN) 2125 H WB Adapter in IL-1 & LPS signal

NAK/TBK1 (CT) 2351 H M WB NF-κB activating kinase 2ndQ 2001

NIK (CT) 1129 H WB NF-κB inducing kinase

SIRPα / SHPS-1 (CT) 1125 H M R WB Tyrosine kinase substrateSTAT1 (CT) 1021 H M R WB, IP JAK activated transcription

HIV and chemokine receptors

Bonzo / STRL33 (CT) 1170 H WB HIV coreceptorBonzo / STRL33 (NT2) 2209 H WB HIV coreceptor

CCR5 (NT) 1112 H WB HIV & chemokine receptor

CCR8 (EL) 2097 H WB HIV & chemokine receptorCX3CR1 / V28 (EL) 2201 H WB HIV & chemokine receptorCX3CR1 / V28 (NT) 2093 H M R WB, FACS HIV & chemokine receptor

CXCR4 (EL) 1012 H M WB HIV & chemokine receptor

CXCR4 (NT) 1009 H M WB, IP, IHC HIV & chemokine receptor

DC-SIGN (CT) 2347 H WB A novel HIV biding protein 3rdQ 2001

DC-SIGN (ED) 2349 H WB A novel HIV biding protein 3rdQ 2001

Eotaxin (CT) 1123 H WB CCR3 ligandGPR15 / BOB (NT) 2101 H M R WB HIV coreceptor


Neurobiology

APP (AβNT) 2133 H M R WB Amyloid protein precursor

APP (CT) 2136 H M R WB Amyloid protein precursorBACE / Asp2 (CT) 2253 H M WB Beta-site APP Cleaving EnzymeBACE2 / Asp1 (NT) 2247 H M R WB Beta-site APP Cleaving Enzyme 2 4thQ 2000BACE2 / Asp1 (CT) 2249 H WB Beta-site APP Cleaving Enzyme 2 4thQ 2000GFRα-1 / GDNFR α (IN) 1133 H M R WB GDNF receptorGFRα-2 / GDNFR β (IN) 1135 H M R WB Neurturin receptorGFRα-3 / GDNFR γ (IN) 1137 H M R WB Persephin receptorNeurturin 1121 H WB Ligand for GFRα-2

P53 Network

Chk2 (NT) 2391 H M R WB A novel check point kinase 2ndQ 2001

MTBP (IN2) 2447 H WB MDM2 binding protein 2ndQ 2001p53DINP1, SIP 3045 H M R WB Apoptosis-associated p53 target New

p53R2 (NT) 2383 H M R WB A novel ribonucleotide reductase 2ndQ 2001

PERP 2451 H WB A mediator of p53 induced apoptosis 2ndQ 2001PID/MTA2 2443 H M R WB Involved in P53 deacetylation 2ndQ 2001

Immunology

APRIL (ED) 2223 H WB A new member in the TNF family 2ndQ 2001

APRIL (ED2) 2415 H M WB A new member in the TNF family 2ndQ 2001BAFF / BLyS / Thank / Tall-1 (CT) 2221 H M R WB B cell Activating Factor in TNF Family

IL-21 (IN) 2465 H WB Interleukin 21, related to IL2 and IL15 3rdQ 2001

IL21R (ED) 2469 H M R WB Interleukin 21 receptor 3rdQ 2001

IL21R (NT) 2471 H M R WB Interleukin 21 receptor 3rdQ 2001

IL22R (NT) 2497 H R WB Interleukin 22 receptor 3rdQ 2001

IL22R (CT) 2489 H R WB Interleukin 22 receptor 3rdQ 2001

TCCR (NT) 2481 H WB A new T cell cytokine receptor 3rdQ 2001TCCR (CT) 2483 H WB A new T cell cytokine receptor 3rdQ 2001

WB-Western blot / IHC-immunohistochemistry / IP-immunoprecipitation / NT-N-terminus

CT-C-terminus / ED-extracellular domain / ID-intracellular domain / IN-intermediate domain

NP-N-terminus of the peptide / CP-C-terminus of the peptide

EL-extracelluar loop / FACS-Flow Cytometry / H-human / M-mouse / R-rat

peptide Product list

13100 Kirkham Way Toll free: 888-513-9525Suite 207 Tel: 858-513-2638Poway, CA 92064 Fax: 858-513-2692www.prosci-inc.com [email protected]

Product Name Cat. No. Source SequencesAcinus (CP) peptide 2215P Human TRTALHGVKWPQSNPKAcinus (CT) peptide 2217P Human SRSTPVRDRGGRRAcinus (IN) peptide 2219P Human DTSENRPENDVPEPPAcinus (NP) peptide 2241P Human DDPVRTAQVPSPPRGKADAM10 (CT) peptide 2051P Human PQRQRPRESYQMGHMRRAIF (CT) peptide 2301P Human KDGEQHEDLNEVAKAIF (IN) peptide 2267P Human PKSATEQSGTGIRSEAIF (NT) peptide 2239P Human KQKKAALSASEGEEApaf-1 (CT) peptide 2015P Human TFYTNGTNLKKIHVSPDFKTApaf-1 (NT) peptide 2013P Human HREALEKDIKTSYIMDHCAPP (AbNT) peptide 2133P Human CDAEFRHDSGYAPP (CT) peptide 2136P Human CGYENPTYKFFEQMQNAPRIL (ED) peptide 2223P Human GTGGPSQNGEGYPAPRIL (ED2) peptide 2415P Human CIRSMPSHPDRAYNSARC (CT) peptide 2081P Human PDPEPEPDFEERDESEDSARC (NT) peptide 2185P Human GNAQERPSETIDRERKRASC peptide 2287P Human RESQSYLVEDLERSBACE (CT) peptide 2253P Human CLRQQHDDFADDISLLKBACE2 (CT) peptide 2249P Human CQRRPRDPEVVNDESSBACE2 (NT) peptide 2247P Human APTPGPGTPAERHADGBAFF (CT) peptide 2221P Human EEGDELQLAIPRENAQBCL-10 (NT) peptide 2161P Human APSLTEEDLTEVKKDCBim (IN) peptide 2065P Human AERPPQLRPGAPTSLQTEPBnip3L (IN) peptide 2289P Human DAQHESGQSSSRGSSHBonzo (CT) peptide 1170P Human KSSEDNSKTFSASHNVEATSBonzo (NT2) peptide 2209P Human GFSSFNDSSQEEHQDCAD (CT) peptide 2011P Mouse KLECDRSRIYRPQTGSCAD (I17) peptide 2007P Mouse YNGSYFDRGAEASSRLCasp-10 (CT) peptide 1128P Human ISAQTPRPPMRRWSSVSCasp-12 (NT) peptide 2325P Murine AARRTHERDPIYKIKGCasp-13 peptide 2245P Human ETVAGPDESVGSAATCasp-9 (IN1) peptide 2071P Human EDIQRAGSGSRRDQARCasp-9 (IN2) peptide 2073P Human ASTSPEDESPGSNPEPDATPCaspase-12 (IN) peptide 2327P Murine CTPSSPSESKRKVEDDECCR5 (NT) peptide 1112P Human SSPIYDINYYTSEPCCCR8 (EL) peptide 2097P Human CYSFYNQQTLKWKIFTNFKChk2 (NT) peptide 2391P Human SRESDVEAQQSHGSSACCIDE-A (CT) peptide 2085P Human DDKEERPSLRSQAKGRFTCIKS/Act1 (CT) peptide 2499P Human REEEYVAPPRGPLPTCIKS/Act1 (NT) peptide 2501P Human PPQLQETRMNRSIPCX3CR1 (EL) peptide 2201P Human CLGDYPEVLQEIWPVCX3CR1 (NT) peptide 2093P Human DQFPESVTENFEYDDLAEACCXCR4 (EL) peptide 1012P Human DRYICDRFYPNDLWVCXCR4 (NT) peptide 1009P Human MEGISIYTSDNYTEDAP kinase 2 peptide 2323P Human ARRKALHPRRRSSTSDaxx (CT) peptide 1163P Human TSVATQCDPEEIIVLSDSDDC-SIGN (CT) peptide 2347P Human CSRDEEQFLSPAPATPNPPPADC-SIGN (ED) peptide 2349P Human CYFMSNSQRNWHDSITADcR1 (ED) peptide 2179P Human CVEEFGANATVETPAAEETDcR1 (ED2) peptide 2299P Human CKEGTFRNENSPE

Peptide List

Product Name Cat. No. Source SequencesDcR2 (ID) peptide 2021P Human GGPERVHRVLFRRRSDcR3 (NT) peptide 2140P Human AETPTYPWRDAETGERCDEDAF peptide 2227P Human TPKGDMSAVNDESFDFF40 (IN) peptide 2155P Human NGSYFDRGAKGGSRLCDFF40 (NT) peptide 2153P Human QKPKSVKLRALRSPRKCDFF40/CAD (I18) peptide 2107P Mouse EGLESRFRNKSGYLRYSDFF45 (CT) peptide 1141P Human KASPPGDLQNPKRARQDPTDFF45/35 (NT) peptide 1148P Human EVTGDAGVPESGEIRTLKPCDNase II (CT) peptide 2059P Human CNGMARKPSRAYKIDR3 (CT) peptide 1120P Human ERMGLDGCVEDLRSRLQRGPDR3 (ED) peptide 1158P Human APCTEPCGNSTCLVCPQDTDR3 (NT) peptide 1166P Human QGGTRSPRCDCAGDFHDR4 (CT) peptide 1139P Human CDSGKFIYLEDGTGSAVSLEDR4 (NT) peptide 1167P Human ASGTEAAAATPSKVWGSSAGDR5 (CT) peptide 2019P Human KIEDHLLSSGKFMYLEGNADDR6 (NT) peptide 2157P Human QPEQKASNLIGTYRHCDRAK1 (NT) peptide 2147P Human EKPGSGGSSPGATSGCDRAK2 (CT) peptide 2149P Human CSKRFRFDDSLPNPHEEotaxin peptide 1123P Human DSMKYLDQKSPTPKPF1Aα peptide 2279P Human DINYQDQIPRTLEEFLASH (CT) peptide 2271P Human SERFQQLMKLFEKSKCFLIP (CT) peptide 1161P Human NGYMYDWNSRVSAKEKYYFLIP (NT) peptide 1159P Human SAEVIHQVEEALDTDEKEFLIPs (CT) peptide 2055P Human QKSLKDPSNNFRMITPYAHGFRα-1 (IN) peptide 1133P Human KNKPLGPAGSENEIGFRα-3 (IN) peptide 1137P Human RFHRQLFSQDWADSGPR15 (NT) peptide 2101P Human YYATSPNSDIRETHSHICAD (CT) peptide 2003P Mouse RRSPLPGEPQRPKRAKRDSSICAD (NT) peptide 2001P Mouse ELSRGASAPDPDDVRPLKPCIKKα (C1) peptide 2025P Human CLGHLSTIIHEANEEQGNSIKKα (C2) peptide 2115P Human TPQDGETSAQMIEENLNIKKα (C3) peptide 2117P Human CTQSSARSLVGSSLEGAIKKβ (C3) peptide 2121P Human CSKVRGPVSGSPDSMNASRIKKε/IKK-i (CT) peptide 2329P Human NRIIERLNRVPAPPDVIKKγ/NEMO (NT) 2335P Human CQYQAPDMDTLQIHVMEIL-1RAcP (C2) peptide 2129P Human KWKGEKSKYPQGRFWKIL-1RAcP (CT) peptide 2131P Human KKSPRRSSSDEQGLSYSSLKNIL-21 (IN) peptide 2465P Human TCPSCDSYEKKPPKEIL-21R (ED) peptide 2469P Human NITDQSGNYSQECGSIL-21R (NT) peptide 2471P Human CPDLVCYTDYLQTIL-22R (CT) peptide 2489P Human DSLFRGLALTVQWESIL-22R (NT) peptide 2497P Human QSSNFENILTWDSGPEIRAK (CT) peptide 1007P Human DRQGPEESDEFQSIRAK-M peptide 2355P Human CSSKFSWDEYEQYKKEIRAK2 (C2) peptide 2123P Human APWAGAATPLLPTENGEGRIRAK2 (CT) peptide 2213P Human READSSSEACVGLEPPQDVTMADD (CT) peptide 1150P Human AAVHSSEEDLRTPPRPVSSmCIDE-A (CT) peptide 2089P Mouse GDTEEQPSPKPSTKGmCIDE-B (CT) peptide 2091P Mouse EGADRWQWHGQRHLHSmFLIP (CT) peptide 1156P Mouse DKVYAWNSGVSSKEKYSmSmac/DIABLO (CT) peptide 2411P Murine SDEGADQEEEAYLREDMyD88 (CT) peptide 2127P Human CTKSWFWTRLAKALSLPMyD88 (IN) peptide 2125P Human CDFQTKFALSLSPGAHDMTBP (IN2) peptide 2447P Human GAVECFEEEDSNSRESLSNAK/TBK1 (CT) peptide 2351P Human ERFGSLTMDGGLRNVD

Product Name Cat. No. Source SequencesNIK (CT) peptide 1129P Human SFAWSWRVKHGQLENRPNTN (NT) peptide 1121P Human DAHSRYHTVHELSAREp53R2 (NT) peptide 2383P Human GDPERPEAAGLDQDERPID/MTA2 peptide 2443P Human PAPSHPASTNEPIVLEDRAIDD (CT) peptide 1117P Human CHNGLRAVEVDPSLLLHMLERAIDD (IN) peptide 1115P Human CAGDRLTGIPSHILNSSPSDRICK (CT) peptide 2183P Human KLKDNKQMGLQPYPERICK (NT) peptide 2075P Human PTIPYHKLADLRYLSRGASGRIP3 peptide 2283P Murine AQFGRGRGWQPFHKSIRPα (CT) peptide 1125P Human KPEPSFSEYASVQVPRKSmac/DIABLO (CT) peptide 2409P Human EERAESEQEAYLREDSODD (NT) peptide 2143P Human SALRRSGYGPSDGPSCSurvivin (CT) peptide 2235P Human CKKVRRAIEQLAAMDSurvivin (NT) peptide 2233P Human MGAPTLPPAWQPSurvivin peptide 2237P Mouse AKTTRQSIEQLAATACE (CT) peptide 1131P Human ASFKLQRQNRVDSKETETRAIL (CT) peptide 1113P Human CTNEHLIDMDHEASFFGAZIPK (IN) peptide 2067P Human RRRNVRGEDSGRKPERRRLK

lysate Product list

13100 Kirkham Way Toll free: 888-513-9525Suite 207 Tel: 858-513-2638Poway, CA 92064 Fax: 858-513-2692www.prosci-inc.com [email protected]

Product Name Catalog No. Application Size

293 whole cell lysate 1210 Western blot 200 µg

3T3 whole cell lysate 1212 Western blot 200 µg

U937 whole cell lysate 1215 Western blot 200 µg

A431 whole cell lysate 1202 Western blot 200 µg

A549 whole cell lysate 1203 Western blot 200 µg

HeLa whole cell lysate 1201 Western blot 200 µg

HepG2 whole cell lysate 1211 Western blot 200 µg

HL60 whole cell lysate 1209 Western blot 200 µg

Jurkat whole cell lysate 1205 Western blot 200 µg

K562 whole cell lysate 1204 Western blot 200 µg

MDA-MB-361 whole cell lysate 1217 Western blot 200 µg

MOLT4 whole cell lysate 1206 Western blot 200 µg

PC-3 whole cell lysate 1216 Western blot 200 µg

Raji whole cell lysate 1207 Western blot 200 µg

SW1353 whole cell lysate 1214 Western blot 200 µg

T24 whole cell lysate 1213 Western blot 200 µg

THP-1 whole cell lysate 1208 Western blot 200 µg

Human brain tissue lysate 1303 Western blot 200 µg

Human heart tissue lysate 1301 Western blot 200 µg

Human kidney tissue lysate 1305 Western blot 200 µg

Human liver tissue lysate 1304 Western blot 200 µg

Human lung tissue lysate 1302 Western blot 200 µg

Human pancreas tissue lysate 1307 Western blot 200 µg

Human small intestine tissue lysate 1308 Western blot 200 µg

Human spleen tissue lysate 1306 Western blot 200 µg

Human brain tumor lysate 1322 Western blot 200 µg

Human breast tumor lysate 1323 Western blot 200 µg

Human colon normal tissue lysate 1341-N Western blot 200 µg

Human colon tumor tissue lysate 1341-T Western blot 200 µg

Human kidney tumor lysate 1324 Western blot 200 µg

Human liver normal tissue lysate 1342-N Western blot 200 µg

Human liver tumor tissue lysate 1342-T Western blot 200 µgHuman lung tumor lysate 1321 Western blot 200 µg

Lysate List

Cell Lysate

Human Tissue Lysate

Human Tumor Tissue Lysate

Product Name Catalog No. Application Size

Mouse brain tissue lysate 1403 Western blot 200 µg

Mouse heart tissue lysate 1401 Western blot 200 µg

Mouse kidney tissue lysate 1405 Western blot 200 µg

Mouse liver tissue lysate 1404 Western blot 200 µg

Mouse lung tissue lysate 1402 Western blot 200 µg

Mouse skeletal muscle tissue lysate 1407 Western blot 200 µg

Mouse small intestine tissue lysate 1408 Western blot 200 µg

Mouse spleen tissue lysate 1406 Western blot 200 µg

Rat brain tissue lysate 1463 Western blot 200 µg

Rat kidney tissue lysate 1465 Western blot 200 µg

Rat liver tissue lysate 1464 Western blot 200 µg

Rat lung tissue lysate 1462 Western blot 200 µg

Rat skeletal muscle tissue lysate 1467 Western blot 200 µgRat spleen tissue lysate 1466 Western blot 200 µg

Rat Tissue Lysate

Mouse Tissue Lysate

Antibody Data Sheets

13100 Kirkham Way Suite 207 Poway, CA 92064 www.prosci- inc.com

Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

techsupport@prosci- inc.com

Anti-IRAK (CT)

CATALOG No.: 1007

BACKGROUND:

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription

factor and an essential mediator of gene expression during

activation of immune and inflammatory responses. NF-κB

mediates the expression of a great variety of genes in

response to extracellular stimuli including IL-1, TNFα and

LPS. A serine/threonine protein kinase associated with IL-1

receptor (IRAK) and its homologue mouse pelle-like protein

kinase (mPLK) were identified recently (1,2). IRAK is

associated with the IL-1 receptor subunits IL-1RI and IL-

1RAcP after IL-1 binding and serves as a signaling molecule

to mediate IL-1 response (3). IRAK mediates a signaling

cascade leading to NF-κB activation by members in IL-1

family including IL-1 and a novel cytokine IL-18 (also termed

IGIF) (1,4).

SOURCE:

Rabbit anti-IRAK polyclonal antibody was raised against a

peptide corresponding to amino acids 700 to 712 of human

IRAK (1).

APPLICATION:

This polyclonal antibody can be used for Western blot at

1:1000 dilution and for immunoprecipitation with 2 to 4 µg

per sample. THP-1 or HeLa whole cell lysate can be used as

positive control and an 80 kDa band should be detected in

the lysate from non-activated cells. It is human, mouse, and

rat reactive, and has no cross response to IRAK2. For

research use only.

STORAGE: It is supplied as purified IgG, 100 µg in 200 µl

of PBS containing 0.02% sodium azide. Store at 4°C, stable

for one year.

Western blot analysis of IRAK

in THP-1 (THP) and HeLa

(HL) whole cell lysates with

anti-IRAK at 1:2000 dilution.

REFERENCES:

1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the

interleukin-1 receptor. Science 1996;271:1128-31.

2. Trofimova M; Sprenkle AB; Green M; Sturgill TW; Goebl MG;

Harrington MA. Developmental and tissue-specific expression of

mouse pelle-like protein kinase. J Bio Chem 1996; 271: 17609-

12.

3. Jianing Huang, Xiong Gao, Shyun Li, and Zhaodan Cao.

Recruitment of IRAK to the interleukin 1 receptor complex requires

interleukin-1 receptor accessory protein. Proc Natl Acad Sci USA

1997;94:12829-12832

4. Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T,

Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does

not drive Th1 development but synergizes with IL-12 for interferon-

gamma production and activates IRAK and NF-κB. Immunity

1997;7:571-581

(RD1299)

13100 Kirkham Way Suite 207 Poway, CA 92064 www.prosci-inc.com

Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CXCR4 (NT) Fusin, LESTR, HUMSTR

CATALOG NO.: 1009

BACKGROUND:

Human immunodeficiency virus (HIV) and related viruses

require coreceptors, in addition to CD4, to infect target cells.

Some G protein-coupled receptors including CCR5, CXCR4,

CCR3, CCR2b and CCR8 in the chemokine receptor family,

and four new human molecules GPR15, STRL33, GPR1 and

V28 were recently identified as HIV coreceptors (1). Among

them, CXCR4 (fusin, LESTR or HUMSTR) is a principal

coreceptor for T-cell tropic strains of HIV-1 fusion and entry

of human white blood cells (2,3). CXCR4 is also required for

the infection by dual-tropic strains of HIV-1 and mediates

CD-4 independent infection by HIV-2 (4,5). The α-chemokine

SDF-1 is the ligand for CXCR4 and prevents infection by T-

tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-

gp120 complex before HIV enters target cells (8). CXCR4

messenger RNA levels correlated with HIV-1 permissiveness

in diverse human cell types (2). Antibodies to CXCR4 block

HIV-1 and HIV-2 fusion and infection of human target cells

(2,5,10). The amino-terminal domain and the second

extracellular loop of CXCR4 serve as HIV binding sites

(10,11).

SOURCE:

Rabbit anti-CXCR-4 polyclonal antibody was raised against a


CXCR4 (3,9).

STORAGE:

It is supplied as purified IgG fraction, 100 µg in 200 µl of

PBS containing 0.02% sodium azide. Store at 4°C, stable for

one year.

APPLICATION:


1:1000 to 1:2000 dilution and for immunoprecipitation and

immunocytochemistry. HeLa whole cell lysate can be used

as positive control. It is human and mouse reactive. For

research use only.

Western blot analysis of

CXCR4 in HeLa total cell

lysate with anti-CXCR4 (NT)

at 1:2000 dilution.

REFERENCES:

1. Dimitrov DS. Cell 1997;91:721-730

2. Feng Y et al. Science 1996;272:872-7.

3. Berson JF et al. J Virol 1996;70:6288-95.

4. Doranz BJ et al. Cell 1996;85:1149-1158

5. Endres MJ et al. Cell 1996;87:745-756

6. Bleul CC et al. Nature 1996;382:829-833

7. Oberlin E et al. Nature 1996;382:833-835

8. Lapham CK et al. Science 1996;274:602-5.

9. Leoetscher M et al. J Biol Chem 1994;269:232-237

10. Brelot A et al. J Virol 1997;71:4744--954751.

11. Lu Z et al. Proc Natl Acad Sci USA 1997;94:6426-6431

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CXCR4 (EL) Fusin, LESTR, HUMSTR

CATALOG NO.: 1012

BACKGROUND:






V28 were recently identified as HIV coreceptors (1). Among

them, CXCR4 (fusin, LESTR or HUMSTR) is a principal

coreceptor for T-cell tropic strains of HIV-1 fusion and entry

of human white blood cells (2,3). CXCR4 is also required for

the infection by dual-tropic strains of HIV-1 and mediates

CD-4 independent infection by HIV-2 (4,5). The α-chemokine

SDF-1 is the ligand for CXCR4 and prevents infection by T-

tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-

gp120 complex before HIV enters target cells (8). CXCR4

messenger RNA levels correlated with HIV-1 permissiveness

in diverse human cell types (2). Antibodies to CXCR4 block

HIV-1 and HIV-2 fusion and infection of human target cells

(2,5,10). The amino-terminal domain and the second

extracellular loop of CXCR4 serve as HIV biding sites

(10,11).

APPLICATION: This polyclonal antibody can be used for

Western blot at 1:500 to 1:1000 dilution. HeLa whole cell

lysate can be used as positive control. It is human and

mouse reactive. For research use only.

SOURCE:

Rabbit anti-CXCR-4 polyclonal antibody was raised

against a peptide corresponding to amino acids 182 to 196

in the second extracellular loop (EL) of human CXCR4

(3,9).


CXCR4 in HeLa total cell

lysate with anti-CXCR4

(EL) at 1:1000 dilution.

STORAGE:


PBS containing 0.02% sodium azide. Store at 4°C, stable

for one year.

REFERENCES: 1. Dimitrov DS. Cell 1997;91:721-730

2. Feng Y et al. Science 1996;272:872-7.

3. Berson JF et al. J Virol 1996;70:6288-95.

4. Doranz BJ et al. Cell 1996;85:1149-1158

5. Endres MJ et al. Cell 1996;87:745-756

6. Bleul CC et al. Nature 1996;382:829-833

7. Oberlin E et al. Nature 1996;382:833-835

8. Lapham CK et al. Science 1996;274:602-5.

9. Leoetscher M et al. J Biol Chem 1994;269:232-237

10. Brelot A et al. J Virol 1997;71:4744--954751

11. Lu Z et al. Proc Natl Acad Sci USA 1997;94:6426-6431

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-STAT1α

CATALOG No.: 1021

BACKGROUND:

STATs (signal transducers and activators of transcription)

are a family of cytoplasmic latent transcription factors that

are activated to regulate gene expression in response to a

large number of extracellular signaling polypeptides

including cytokines, interferons, and growth factors. After

phosphorylation by JAK tyrosine kinases, STATs enter the

nucleus to regulate transcription of many different genes.

Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a,

Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a

wide activation profile (1,2). STAT1 is activated by many

different ligands including IFN family (IFN-α, IFN-β, IFN-γ

and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-

CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-

1) (3). STAT1 has two forms, the 91 kDa STAT1α and the

84 kDa STAT1β which are encoded by the same gene with

splicing variant (4).

SOURCE:

Rabbit anti-STAT1α polyclonal antibody was raised against

a peptide corresponding to amino acids 712 to 750 of human

STAT1α (4). The sequences differ from the murine

corresponding sequences by four amino acids.

APPLICATION:


1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 µg

per sample. It is human, mouse, and rat reactive. Whole cell

lysate from HeLa, Jurkat, or A431 cells can be used as

positive control and a 91 kDa band can be detected. No

reaction to the 84 kDa STAT1β. For research use only.

STORAGE:

It is supplied as purified IgG, 100 µg in 200 µl of PBS

containing 0.02% sodium azide. Store at -20°C. Stable for

one year at 2-8°C.

Western blot analysis

of STAT1α in whole

cell lysates from HeLa

(H), Jurkat (J), A431

(A), K562 (K), and

NIH3T3 (N) cells,

respectively, with anti-

STAT1α at 1:1000

dilution.

REFERENCES:

1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological

implications. Annu Rev Immunol 1998;16:293-322.

2. Schindler C, Darnell JE Jr. Transcriptional responses to

polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem

1995;64:621-51.

3. Darnell JE Jr. STATs and gene regulation. Science

1997;277:1630-5.

4. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr.

Proteins of transcription factor IS GF-3: one gene encodes the 91-

and 84-kDa ISGF-3 proteins that are activated by interferon alpha.

Proc Natl Acad Sci USA 1992;89:7836

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-p62 dok

CATALOG No.: 1107

BACKGROUND:

Signals from most growth factors and cytokines are

transduced by receptor tyrosine kinases or non-receptor

tyrosine kinases. Activated tyrosine kinases phosphorylate

their substrates, which mediate the cellular response to

extracellular stimuli. A long-sought major substrate termed

p62dok (downstream of tyrosine kinase) for many tyrosine

kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and

activated EGF, PDGF, IGF, VEGF and insulin receptors was

identified recently from human and mouse by several

laboratories (1,2). Upon phosphorylation, p62dok forms a

complex with the ras GTPase-activating protein (RasGAP)

(1-3). p62dok represents a new family with very recently

identified p56dok (4)

SOURCE:

Rabbit anti-p62dok polyclonal antibody was raised against a


p62dok (1).

APPLICATION:

This polyclonal antibody can be used for detection of p62dok

expression by Western blot at 1:1000 to 1:2000 dilution.

Whole cell lysate from Jurkat cells can be used as positive

control and a 62 kDa band should be detected. This antibody

is for research use only.

STORAGE:

It is supplied as affinity purified IgG with immunogenic

peptide, 100 µg in 200 µl of PBS containing 0.02% sodium

azide. Store at -20°C. Stable for one year at 2-8°C.


p62dok in Jurkat (Jur) and

THP-1 (THP) total cell

lysates with anti-p62dok at

1:1000 dilution.

REFERENCES: 1. Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R,

Stillman B, Clarkson B p62(dok): a constitutively tyrosine-

phosphorylated, GAP-associated protein in chronic myelogenous

leukemia progenitor cells. Cell 1997;88:197-204.

2. Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-

associated 62 KDa protein as a docking protein, Dok. Cell

1997;88:205-211.

3. Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley

LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane

tyrosine residues couple the Eph family receptor EphB2/Nuk to

specific SH2 domain proteins in neuronal cells. EMBO J

1997;16:3877-3888.

4. Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi

R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD.

Molecular cloning and characterization of p56(dok-2) defines a new

family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-

4830.

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CCR5 (NT)

CATALOG NO.: 1112

BACKGROUND:

Human immunodeficiency virus (HIV) and related virus





V28 were recently identified as HIV coreceptors (1,2).

Among them, CCR5 (CC-CKR-5) is a principal coreceptor for

macrophage- and dual-tropic HIV-1 strains fusion and entry

of human white blood cells (3,4). CCR5 is required for the

infection by HIV-1, HIV-2, and SIV (1,2). The β-chemokines

RANTES, MIP-1α and MIP-1β are the ligands for CCR5 and

prevent infection by M-tropic HIV-1 (3-5). CXC5 associates

with the surface CD4-gp120 of HIV complex and leads to

membrane fusion and virus entry of target cells (6,7). The

amino-terminal domain and the extracellular loops of CCR5

serve as HIV biding sites (8,9). CCR5 messenger RNA is

expressed in lymphoid organs and monocytes.

SOURCE:

Rabbit anti-CCR5 polyclonal antibody was raised against a


CCR5 (10,11).

STORAGE:


containing 0.02% sodium azide. Store at 4°C, stable for one

year.

APPLICATION:


1:1000 to 1:2000 dilution. THP-1 whole cell lysate can be

used as positive control and an approximate 40 kDa band

can be detected. For research use only.


CCR5 in THP-1 whole cell

lysate with anti-CCR5 (NT)

at 1:1000 dilution.

REFERENCES:

1. Dimitrov, DS. Cell 1997;91:721-30

2. Littman, DR. Cell 1998;93:677-80

3. Deng, H, et al. Nature 1996;381:661-6

4. Dragic, T, et al. Nature 1996;381:667-73

5. Coochi, F. et al. Science 1995;270:1811-5.

6. Wu, L. et al. Nature 1996;384:179-83

7. Trkola , A. et al. Nature 1996;384:184-7

8. Doranz, BJ. et al. J Virol 1997;71:6305-14

9. Dragic, T, et al. J Virol 1998;72:279-85

10. Raport, CJ, et al. J Biol Chem 1996;271:17161-6

11. Combadiere, C. et al. J Leuk Biol 1996;60:147-52

(RD0900)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-TRAIL (CT) Apo-2L

CATALOG No.: 1113

BACKGROUND:

Apoptosis, or programmed cell death, occurs during normal

cellular differentiation and development of multicellular

organisms. Apoptosis is induced by certain cytokines

including TNF and Fas ligand in the TNF family through their

death domain containing receptors, TNFR1 and Fas. A novel

member in the TNF family was recently identified and

designated TRAIL (for TNF-related apoptosis-inducing

ligand) and Apo-2L (for Apo-2 ligand)1,2. TRAIL is a type II

membrane protein and expressed in a variety of human

tissues. Two novel death domain containing receptors DR4

and DR5 have been identified as the receptor for TRAIL3-6.

Like TNF and Fas ligand, TRAIL induces apoptosis and NF-

κB activation in many tissues and cells.

SOURCE:

Rabbit anti-TRAIL (CT) polyclonal antibody was raised


of human TRAIL (1).

APPLICATION:

This polyclonal antibody can be used for detection of TRAIL

by Western blot at 1:500 dilution. For research use only.

STORAGE:

It is supplied as ion chromatography purified IgG, 100 µg in

200 µl of PBS containing 0.02% sodium azide. Store at 4°C,

stable for one year. Azide free antibody is available.


TRAIL in HeLa cell lysate

containing 10, 2.5, or 1 ng of

recombinant protein

containing extracellular

domain of TRAIL with anti-

TRAIL (CT) at 1 µg/ml.

REFERENCES:

1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl

JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al.

Identification and characterization of a new member of the TNF

family that induces apoptosis. Immunity 1995;3:673-682

2. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A;

Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new

member of the tumor necrosis factor cytokine family. J. Biol. Chem.

1996;271:12687-90

3. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R;

Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand

TRAIL. Science; 1997;276:111-113

4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K,

Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2

(DR5) signal FADD-dependent apoptosis and activate NF-κB.

Immunity 1997;7:831-836

5. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist

decoy receptor and a death domain-containing receptor for TRAIL.

Science 1997;277:815-818

6. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M,

Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI,

Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced

apoptosis by a family of signaling and decoy receptors. Science

1997;277:818-821

(RD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-RAIDD (IN) CRADD

CATALOG No.: 1115

BACKGROUND:




including TNF and Fas ligand of the TNF family through their

death domain (DD)-containing receptors, TNFR1 and Fas.

The death signals are transduced by a group of DD-

containing adapter molecules. A novel cell death adapter

was recently identified by two independent groups and

designated RAIDD (RIP-associated ICH-1/CED-3-

homologous protein with DD) and CRADD (caspase and RIP

adapter with DD)1,2. RAIDD contains a DD and a CARD (for

caspase recruitment domain) which interact with RIP and

caspase, respectively, to transduce death signals1,3. RAIDD

is constitutively expressed in many tissues and mediates

apoptosis caused by Fas and TNFR-1.

SOURCE:

Rabbit anti-RAIDD (IN) polyclonal antibody was raised

against a peptide corresponding to amino acids 99 to 117 of

human RAIDD1.

APPLICATION:

This polyclonal antibody can be used for detection of RAIDD

by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate

from HeLa or K562 cells can be used as positive control and

a 22 kDa band should be detected. This antibody is for

research use only.

STORAGE:

It is supplied as affinity chromatography purified IgG, 100

µg in 200 µl of PBS containing 0.02% sodium azide. Store

at 4°C, stable for one year.


RAIDD in whole cell

lysates from HeLa (H) or

K562 (K) cells with anti-

RAIDD (IN) at 1:500

dilution.

REFERENCES:

1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule.

Nature 1997;385:86-89

2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G,

Fernandes-Alnemri T, Alnemri ES. CRADD, a novel human

apoptotic adaptor molecule for caspase-2, and FasL/tumor

necrosis factor receptor-interacting protein RIP. Cancer Res 1997

57:615-619

3. Hofmann K, Bucher P, Tschopp J. The CARD domain: a new

apoptotic signalling motif. Trends Biochem Sci 1997;22:155-15

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-RAIDD (CT) CRADD

CATALOG No.: 1117

BACKGROUND:





death domain (DD)-containing receptors, TNFR1 and Fas.

The death signals are transduced by a group of DD-

containing adapter molecules. A novel cell death adapter

was recently identified by two independent groups and

designated RAIDD (RIP-associated ICH-1/CED-3-

homologous protein with DD) and CRADD (caspase and RIP

adapter with DD)1,2. RAIDD contains a DD and a CARD (for

caspase recruitment domain) which interact with RIP and

caspase, respectively, to transduce death signals1,3. RAIDD

is constitutively expressed in many tissues and mediates

apoptosis caused by Fas and TNFR-1.

SOURCE:

Rabbit anti-RAIDD (CT) polyclonal antibody was raised


of human RAIDD1.

APPLICATION:

This polyclonal antibody can be used for detection of RAIDD


from HeLa cells can be used as positive control and a major

22 kDa band should be detected. This antibody is for

research use only.

STORAGE:



for one year.


RAIDD in HeLa total cell lysate

with anti-RAIDD (CT) at 1:500

dilution.

REFERENCES:

1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor

molecule. Nature 1997;385:86-89

2. Ahmad M, Srinivasula SM, Wang L, Talanian RV,

Litwack G, Fernandes-Alnemri T, Alnemri ES. CRADD, a

novel human apoptotic adaptor molecule for caspase-2,

and FasL/tumor necrosis factor receptor-interacting protein

RIP. Cancer Res 1997 57:615-619

3. Hofmann K, Bucher P, Tschopp J. The CARD domain: a

new apoptotic signalling motif. Trends Biochem Sci

1997;22:155-15

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR3 (CT) Wsl-1, Apo-3, TRAMP, LARD

CATALOG No.: 1120

BACKGROUND:






cell death receptor was recently identified by several groups

independently and designated DR3, Wsl-1, Apo-3, TRAMP

and LARD1-5. The ligand for this novel cell death receptor

has not yet been defined. DR3 is highly expressed in the

tissues enriched in lymphocytes including PBL, thymus and

spleen. Like TNFR1, DR3 induces apoptosis and NF-κB

activation.

SOURCE:

Rabbit anti-DR3 (CT) polyclonal antibody was raised against


DR31,2.

APPLICATION:


1:500 to 1:1000 dilution. Jurkat total cell lysate can be used

as positive control and a 59 kDa band should be detected. It

is human, mouse, and rat reactive and has no cross reaction

to other death receptors. For research use only.

STORAGE:



for one year.


DR3 in Jurkat total cell

lysate with anti-DR3 (CT)

at 1:500 dilution.

REFERENCES:

1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan

DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.

2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT;

Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.

3. Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL;

Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.

4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V;

Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al.

Immunity, 1997;6:79-88.

5. Screaton GR; Xu XN; Olsen AL; Cowper AE; Tan R; McMichael

AJ; Bell JI. Proc. Nat. Acad. Sci. USA. 1997;94:4615-9.

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti- Neurturin

CATALOG No.: 1121

BACKGROUND:

Glial cell line-derived neurotrophic factor (GDNF) plays key

roles in the control of vertebrate neuron survival and

differentiation. A novel neurotrophic factor was recently

cloned from human and mouse and designated neurturin (1,

2). Physiological responses to neurturin (NTN) require the

presence of receptor tyrosine kinase RET and a novel

glycosylphosphatidylinositol linked receptor NTNRα (3,4).

The cDNAs encoding NTNRα from human, rat, chicken, and

mouse have been cloned recently (3-8) and termed

GDNFRβ, Ret ligand 2 (RETL2) or TGF-β-related

neurotrophic factor receptor 2 (TrnR2) and nominated as

GFRα-2 recently (9). NTN binds to and forms a complex with

GFRα-2 and the Ret PTK and activates the RET receptor

tyrosine kinase pathway. Both NTN and GDNF can activate

the MAP kinase and phosphatidylinositol 3-kinase pathways

and play a critical role in the development of many neuronal

populations. Neurturin and GDNF define a new family of

neurotrophic factors.

SOURCE:

Rabbit anti-neurturin polyclonal antibody was raised against


neurturin (1).

APPLICATION:

This polyclonal antibody can be used for detection of

neurturin by Western blot. An approximate 14 kDa band of

the full length recombinant NTN was detected. For research

use only.

STORAGE:

It is supplied as immunoaffinity purified IgG, 100 µg in 200

µl of PBS containing 0.02% sodium azide. Store at -20°C.

Stable for one year at 2-8°C.

Western blot analysis of NTN in

HeLa cell lyaste containing 10 or

2.5 ng of full length recombinant

NTN with anti-neurturin at 1:500.

REFERENCES: 1. Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470 2. 2. Heuckeroth RO, Kotzbauer P, Copeland NG, et al. Neurturin, a novel neurotrophic factor, is localized to mouse chromosome 17 and human chromosome 19p13.3. Genomics 1997;44(1):137-40 3. Klein RD, Sherman D, Ho WH, et al. GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997;387:717-721 4. Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724 5. Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-802 6. Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci USA 1997;94:6238-6243 7. Suvanto P, Wartiovaara K, Lindahl M, et al. Cloning, mRNA distribution and chromosomal localisation of the gene for glial cell line-derived neurotrophic factor receptor beta, a homologue to GDNFR-alpha. Hum Mol Genet 1997;6:1267-1273 8. Wang CY, Ni J, Jiang H, et al. Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B: a novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. Neuroscience 1998;83(1):7-14 9. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFRα Neuron 1997;19:485 (RD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Eotaxin

CATALOG No.: 1123

BACKGROUND:

Chemokines play a key role in inflammation. The CC

chemokine eotaxin is a potent and specific eosinophil

chemoattractant that is expressed by a variety of cell types

in certain inflammatory conditions (1,2). Some G-protein

coupled chemokine receptors are also utilized as virus

coreceptors for fusion and infection of cells. The eotaxin

receptor CCR3 is required for HIV-1 entry into target cells

such as microglia and eotaxin inhibits the infection of HIV-1

(3,4).

SOURCE:

This polyclonal antibody was raised in rabbits against a


eotaxin (1,2).

APPLICATION:

This polyclonal antibody can be used for detection of eotaxin

by Western blot at 0.5 to 1 µg/ml. The recombinant protein

can be used as positive control and monomer and

homodimer are detected at approximate 9 and 18 kDa. For

research use only.

STORAGE:



year.

RELATED PRODUCTS:

Blocking peptide, 50 µg at 200 µg/ml, is available for

competition studies (Catalog No. 1123P).


eotaxin in HeLa cell lysate

containing 10, 2.5, or 1 ng of

full length recombinant

eotaxin with anti-eotaxin at 1

µg/ml.

REFERENCES: 1. Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30 2. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-12 3. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997;385(6617):645-9 4. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV -1 isolates. Cell. 1996;85:1135-48

(WD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-SIRPα (CT) SHPS-1, MyD-1, BIT, p84

CATALOG No.: 1125

BACKGROUND:

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2

are critical regulators in the intracellular signaling pathways

that result in cell responses such as mitosis, differentiation,

migration, survival, transformation or death. SHP-2 is a

signal transducer for several receptor tyrosine kinases and

cytokine receptors. A novel SHP-2 associated glycoprotein

was recently cloned from human, rat, mouse and cattle by

several labs and was designated SIRPα (1), SHPS-1 (2,3),

MyD-1 (4), BIT (5,6) and p84 (7). SIRPα is a new gene

family containing at least fifteen members. SIRPα is a

substrate of many activated tyrosine kinases such as insulin

receptor, EGFR, PDGFR and src, and a specific docking

protein for SHP-2 (1,2,5,8). SIRPα has regulatory effects on

cellular responses induced by serum, growth factors, insulin,

oncogenes, growth hormones and cell adhesion and plays a

general role in different physiological and pathological

processes (1,2,5,8).

SOURCE:

Rabbit anti-SIRPα polyclonal antibody was raised against a


SIRPα1 (1). The sequences of immunogenic peptide differ

from those of mouse, rat and bovine by one amino acid (1-

7).

APPLICATION:


1:1000 to 1:2000 dilution. Whole cell lysate from THP-1

cells can be used as positive control and 75-110 kDa bands

should be detected. It is human, mouse, and rat reactive

and recognizes SIRPα1, 2 and 3. For research use only.


SIRPα in THP-1 whole

cell lysate with anti-

SIRPα at 1:2000

dilution.

STORAGE:



year.

REFERENCES:

1. Kharitonenkov A, et al. Nature 1997;386:181-186.

2. Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899

3. Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67

4. Brooke GP, et al. Eur J Immunol 1998;28:1-11

5. Ohnishi H, et al. J Biol Chem 1996;271:25569-25574

6. Ohnishi H, et al. Genomics 1997;40:504-506

7. Comu S, et al. J Neurosci 1997;17:8702-8710

8. Stofega MR, et al. J Biol Chem 1998;273:7112-7117

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-10 (CT) FLICE2

CATALOG No.: 1128

BACKGROUND:

Apoptosis is related to many diseases and induced by a

family of cell death receptors and their ligands. Cell death

signals are transduced by death domain (DD)- containing

adapter molecules and members of the ICE/CED-3 protease

family. A novel ICE/CED-3 protease was identified recently,

designated FLICE2 and Mch41,2 and renamed as caspase-

10. Caspase-10 has two death effector domains (DEDs) that

bind to the DED in the adapter molecule FADD and recruits

both TNFR1 and CD95 to form complexes with these

receptors. Caspase-10 is therefore involved in the CD95 and

TNFR1 induced apoptosis1. Caspase-10 cleaves and

activates caspase-3, -4, -6, -7, -8 and -9, which causes the

proteolytic cleavage of many key proteins such as PARP.

Cleavage of PARP occurs in many different systems during

apoptosis and is the hallmark of programmed cell death3.

Caspase-10 is expressed in many tissues and cell lines1,2.

SOURCE:

Rabbit anti-caspase-10 polyclonal antibody was raised


of human FLICE21.

APPLICATION:


caspase-10 by Western blot at 1:500 to 1:1000 dilution.

HeLa whole cell lysate can be used as positive control and a

59 kDa band can be detected. This antibody only recognizes

FLICE2 form of caspase-10. It is human, mouse and rat

reactive. For research use only.


caspase-10 in HeLa (H), Jurkar

(J), A431 (A), K562 (K), and

NIH3T3 (3T3) whole cell

lysates with anti-caspase-10 at

1:1000 dilution.

STORAGE:



for one year.

REFERENCES:

1. Vincenz.C. and Dixit,V.M. Fas-associated death domain protein

interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3

homologue, is proximally involved in CD95- and p55-mediated

death signaling. J. Biol. Chem. 1997;272:6578-6583

2. Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM,

Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G,

Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a

novel human apoptotic cysteine protease containing two FADD-like

domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469

3. Cohen GM. Caspases: the executioners of apoptosis. Biochem J

1997;326:1-16

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-NIK (CT)

CATALOG No.: 1129

BACKGROUND:





response to extracellular stimuli including IL-1, TNFα, LPS

and mitogens. A serine/threonine protein kinase which

mediates NF-κB activation by IL-1, TNFα and CD95 was

identified recently and designated NIK (for NF-κB inducing

kinase) (1). NIK is an activator of IκB kinase alpha and beta

(IKKα and IKKβ) (2-5). Therefore, NIK is a key molecule in

the NF-κB signaling pathway leading to the induction of a

variety of gene expression in response to proinflammatory

cytokines and bacteria products.

SOURCE:

Rabbit anti-NIK polyclonal antibody was raised against a


NIK (1).

APPLICATION:

This polyclonal antibody can be used for detection of NIK by

Western blot. 293 cell lysate can be used as positive control.

STORAGE:

It is supplied as 100 µg purified IgG in 200 µl of PBS


year.

RELATED PRODUCT:

Blocking peptide, 50 µg/250 µl, is available for competition

studies (Catalog No. 1129P).

293 cell lysate, 200 µg/100 µl, is available for positive

control (Catalog No. 1210).

REFERENCES:

1. Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3K-

related kinase involved in NF-kappaB induction by TNF, CD95 and

IL-1. Nature 1997;385:540-544

2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.

Identification and characterization of an IkappaB kinase. Cell

1997;90:373-383

3. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. IkappaB

kinase-beta: NF-kappaB activation and complex formation with

IkappaB kinase-alpha and NIK. Science 1997;278:866-869

4. Ling L, Cao Z, Goeddel D. NF-kappaB -inducing kinase activates

IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci USA

1998;95:3792-3797

5. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita

H, Okumura K. Differential regulation of IkappaB kinase alpha and

beta by two upstream kinases, NF-kappaB-inducing kinase and

mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl

Acad Sci USA 1998;95:3537-3542 (RD0400)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-TACE (CT) ADAM17

CATALOG No.: 1131

BACKGROUND:

Tumor-necrosis factor-α is a proinflammatory cytokine and

contributes to a variety of inflammatory disease responses

and programmed cell death. TNF-α is synthesized as a 26K

type II membrane-bound precursor that is cleaved by a

convertase to generate secreted 17K mature TNF-α. TNF-α

converting enzyme (TACE) protein was recently purified and

the human and mouse TACE cDNAs were cloned by several

groups separately (1-3). TACE is a membrane-bound

metalloprotease-disintegrin in the family of mammalian

ADAM (for a disintegrin and metalloprotease). TACE also

processes other cell surface proteins, including TNF

receptor, TGFα, the L-selectin adhesion molecule, and

alpha-cleavage of amyloid protein precursor (APP) (4,5).

TACE mRNA is expressed in a variety of human and murine

tissues. TACE was selected as one of the few targets in

cytokine activation by the Eighth International Conference of

the Inflammation Research Association (6)

SOURCE:

Rabbit anti-TACE (CT) polyclonal antibody was raised


of human TACE (1,2). This sequence differs from those of

mouse and rat TACE by one amino acid (3).

APPLICATION:

This polyclonal antibody can be used for detection of TACE

by Western blot at 1:500 to 1:2000 dilution. HeLa or Jurkat

whole cell lysate can be used as positive control and 80 to

130 kDa bands can be detected, which may represent

mature protein, precursor, and glycosylated TACE. It is

human, mouse, and rat reactive. For research use only.

STORAGE:

It is supplied as immunoaffinity chromatography purified

IgG, 100 µg in 200 µl of PBS containing 0.02% sodium

azide. Store at -20°C, stable for one year.


TACE in HeLa (1,3) and

Jurkat (2,4) whole cell lysate

in the absence (1,2) or

presence (3,4) of blocking

peptide (Catalog no. 1131P)

with anti-TACE (CT) at 1:500

dilution.

REFERENCES:

1. Black RA, Rauch CT, Kozlosky CJ, et al. A

metalloproteinase disintegrin that releases tumour-necrosis

factor-alpha from cells. Nature 1997;385:729-733

2. Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin

metalloproteinase that processes precursor tumour-necrosis

factor-alpha. Nature 1997;385:733-736

3. Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of

mouse tumor necrosis factor-alpha converting enzyme (TACE)

and partial analysis of its promoter. Gene 1999;233:67-74

4. Peschon JJ, Slack JL, Reddy P, et al. An essential role for

ectodomain shedding in mammalian development. Science

1998;282:1281-4

5. Buxbaum JD, Liu KN, Luo Y, et al. Evidence that tumor

necrosis factor alpha converting enzyme is involved in

regulated alpha-secretase cleavage of the Alzheimer amyloid

protein precursor. J Biol Chem 1998;273:27765-7

6. Giegel DA, Chaplin DD. Targets in cytokine activation.

Agents Actions Suppl 1998;49:1-3 (WD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti- GFRα-1 GDNFRα, RETL1, TrnR1

CATALOG No.: 1133 BACKGROUND: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRα, which is a cell surface receptor for GDNF (1,2). The cDNAs encoding GDNFRα from human, rat, chicken and mouse have been cloned recently (1-5). GDNFRα was also termed Ret ligand 1 (RETL1) or TGF-β-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently (5-7). GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function. SOURCE: Rabbit anti- GFRα-1 polyclonal antibody was raised against a peptide corresponding to amino acids 369 to 382 of human GFRα-1 (1). APPLICATION: This polyclonal antibody can be used for detection of GFRα-1 by Western blot. The amino acid sequence of immunogenic peptide is identical to those of mouse and rat and the antibody recognizes GFRα-1 from human, mouse and rat origins. This antibody is for research use only. STORAGE: It is supplied as purified IgG, 100 µg in 200 µl of PBS containing 0.02% sodium azide. Store at -20°C. Stable for one year at 2-8°C.

Western blot analysis of GFRα-1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with anti-GFRα−1 at 1:500 dilution.

REFERENCES: 1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z;

Cupples R; Louis JC; Hu S; Altrock BW; Fox GM. GDNF-induced activation

of the Ret protein tyrosien kinase is mediated by GDNFR-α, a novel receptor

for GDNF. Cell, 1996;85:1113-24.

2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD;

Gray C; Armanini MP; Pollock RA; Herti F; Phillips HS; Goddard A; Moore

MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson

CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF.

Nature, 1996;382:80-83.

3. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L,

Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL.

Glial cell line-derived neurotrophic factor-dependent RET activation can be

mediated by two different cell-surface accessory proteins. Proc Natl Acad

Sci U S. 1997;94:6238-6243

4. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A,

Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires

a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature

1997;387:721-724

5. Dey,B.K., Wong,Y.W. and Too,H.P. Cloning of a novel murine isoform of

the glial cell line-derived neurotrophic factor receptor. Neuroreport

1998;9:37-42

6. Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO, Keck

CL, ZimonjicDB, Popescu NC, Johnson EM Jr, Milbrandt J. TrnR2, a novel

receptor that mediates neurturin and GDNF signaling through Ret. Neuron

1997;18:793-802

7. Nomenclature Committee. Nomenclature of GPI-linked receptors for the

GDNF ligand family. GFRα.. Neuron 1997;19:485 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti- GFRα−2, GDNFRβ, RETL2, TrnR2, NTNR-α

CATALOG No.: 1135

BACKGROUND:

Members of the glial cell line-derived neurotrophic factor

(GDNF) family, including GDNF and neurturin (NTN) (1),

play key roles in the control of vertebrate neuron survival

and differentiation. Physiological responses to NTN require

the presence of a novel glycosylphosphadidylinositol linked

protein NTNRα, which is a cell surface receptor for NTN

(2,3). The cDNAs encoding NTNRα from human, rat,

chicken, and mouse have been cloned recently (2-7).

NTNRα was also termed GDNFRβ, Ret ligand 2 (RETL2) or

TGF-β-related neurotrophic factor receptor 2 (TrnR2) (4-7)

and nominated as GFRα-2 recently (8). GFRα-2 binds NTN

and mediates activation of RET receptor tyrosine kinase by

both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK

form a complex to transduce NTN signal and to mediate

NTN function.

SOURCE:

Rabbit anti-GFRα-2 polyclonal antibody was raised against

a peptide corresponding to amino acids 377 to 391 of

human GFRα-2. The amino acid sequences of immunogenic

peptide are identical to those of mouse and rat.

APPLICATION:

This polyclonal antibody can be used for detection of GFRα-

2 by Western blot at 1:1000 to 1:2000 dilution. HeLa whole

cell lysate can be used as positive control and a 52 kDa

band should be detected. It is human, mouse, and rat


STORAGE: It is supplied as purified IgG, 100 µg in 200 µl of PBS


one year at 2-8°C.

Western blot analysis of GFRα-2

in HeLa total cell lysate with anti-

GFRα-2 at 1:1000 dilution.

REFERENCES: 1. Kotzbauer PT, Lampe PA, Heuckeroth RO, Golden JP, Creedon DJ, Johnson EM Jr, lbrandt J. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470 2. Klein RD, Sherman D, Ho WH, Stone D, Bennett GL, Moffat B, Vandlen R, Simmons L, Gu Q, Hongo JA, Devaux B, Poulsen K, Armanini M, Nozaki C, Asai N, Goddard A, Phillips H, Henderson CE, Takahashi M, Rosenthal A. A GPI-link ed protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997;387:717-721 3. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A, Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724 4. Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO, Keck CL, Zimonjic DB, Popescu NC, Johnson EM Jr, Milbrandt J. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-802 5. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L, Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci USA 1997;94:6238-6243 6. Suvanto P, Wartiovaara K, Lindahl M, Arumae U, Moshnyakov M, Horelli-Kuitunen N, Airaksinen MS, Palotie A, Sariola H, Saarma M. Cloning, mRNA distribution and chromosomal localisation of the gene for glial cell line-derived neurotrophic factor receptor beta, a homologue to GDNFR-alpha. Hum Mol Genet 1997;6:1267-1273 7. Wang CY, Ni J, Jiang H, Hsu TA, Dugich-Djordjevic M, Feng L, Zhang M, Mei L, Gentz R, Lu B. Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B: a novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. Neuroscience 1998 Mar;83(1):7-14 8. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFRα. Neuron 1997;19:485 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti- GFRα-3, GDNFRγ, RETL2, TrnR2, NTNR-α

CATALOG No.: 1137

BACKGROUND:

Members of the glial cell line-derived neurotrophic factor

(GDNF) family including GDNF and neurturin (NTN) play

essential roles in the control of vertebrate neuron survival

and differentiation. A new member of the GDNF family was

recently identified and designated persephin (1).

Physiological responses to these neurotrophic factors

require two receptor subunits, the novel

glycosylphosphadidylinositol linked protein GFRα and Ret

receptor tyrosine kinase GFRβ (2). Following the findings of

GFRα-1 and -2, a novel receptor in GFRα family was

identified very recently from human and mouse and

designated GFRα-3 (3-7). GFRα-3 binds persephin, thus,

persephin, GFRα-3, and Ret PTK form a complex to

transduce persephin signal and to mediate persephin

function.

SOURCE:

Rabbit anti-GFRα-3 polyclonal antibody was raised against

a peptide corresponding to amino acids from 347 to 360 of

murine GFRα-3 (6).

APPLICATION:

This polyclonal antibody can be used for detection of GFRα-

3 expression by Western blot at 1:500 to 1:1000 dilution.

Murine kidney, liver or heart tissue lysates can be used as

positive control. It is human mouse, and rat reactive. For

research use only.

STORAGE:



one year at 2-8°C.

Western blot analysis of GFRα−3 in crude cell membrane fractions of murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with anti-GFRα−3 at 1:500 dilution.

REFERENCES: 1. Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a

novel neurotrophic factor related to GDNF and neurturin. Neuron

1998;20:245-253.

2. Nomeclature Committee. Nomeclature of GPI-linked receptors

for the GDNF ligand family. GFRα. Neuron 1997: 19:485.

3. Jing S. Yu Y, Fang M, Hu Z, Holst PL, Boone T, Delaney J.

Schultz H, Zhou R, Fox GM. GFRalpha-2 and GFRalpha-3 are two

new receptors for ligands of the DGNF family. J Biol Chem 1997;

272:33111-33117

4. Baloh RH, Gorodinsky A, Golden JP, Tansey MG, Keck CL,

Popescu NC, Johnson EM Jr, Milbrandt J. GFRalpha3 is an orphan

member of the GDNF/neurturin/persephin receptor family. Proc Natl

Acad Sci USA 1998;95:5801-5806.

5. Worby CA, Vega QC, Chao HH, Seasholtz AF, Thompson RC,

Dixon JE. Identification and characterization of GFRalpha-3, a

novel Co-receptor belonging to the glial cell line-derived

neurotrophic receptor family. J Biol Chem 1998;273:3502-3508.

6 Naveilhan P, Baudet C, Mikaels A, Shen L, Westphal H, Ernfors

P. Expression and regulation of GFRalpha3, a glial cell line-derived

neurotrophic factor family receptor. Proc Natl Acad Sci USA 1998;95:1295-1300.

7. Nomoto,S., Ito,S., Yang,L.X. and Kiuchi,K. Molecular cloning and

expression analysis of GFR alpha-3, a novel cDNA related to

GDNFR alpha and NTNR alpha. Biochem. Biophys. Res. Commun.

1998;244:849-853. (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR4 (CT) TRAIL-R1

CATALOG No.: 1139

BACKGROUND:





death domain containing receptors, TNFR1 and Fas. A

novel death domain containing receptor was recently

identified and designated DR4 (for death receptor 4)1. The

ligand for this novel death receptor has been identified and

termed TRAIL2,3, which is a new member in the TNF family.

DR4 is also called TRAIL receptor-1 (TRAIL-R1)4. DR4 is

expressed in most of human tissues including spleen,

peripheral blood leukocytes, small intestine and thymus.

Like TNFR1, Fas and DR3, DR4 mediates apoptosis and

NF-κB activation in many tissues and cells.

SOURCE:

Rabbit anti-DR4 (CT) polyclonal antibody was raised against

a peptide corresponding to amino acids 427 to 445 of

human DR4 mature protein.

APPLICATION:

This polyclonal antibody can be used for detection of DR4

expression by Western blot at 1:500 to 1:1000 dilution. HeLa

whole cell lysate can be used as positive control and a 57

kDa band should be detected. It is human, mouse, and rat

reactive and has no cross reaction to DR5. For research use

only.

STORAGE:



year.

Western blot analysis of DR4

in HeLa total cell lysate with

anti-DR4 (CT) at 1:500

dilution.

REFERENCES:

1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner

R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL.

Science; 1997;276:111-113

2. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl

JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al.

Identification and characterization of a new member of the TNF

family that induces apoptosis. Immunity 1995;3:673-682

3. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A;

Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member

of the tumor necrosis factor cytokine family. J. Biol. Chem.

1996;271:12687-90

4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K,

Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2

(DR5) signal FADD-dependent apoptosis and activate NF-κB.

Immunity 1997;7:831-836

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DFF45 (CT)

CATALOG No.: 1141

BACKGROUND:



signals are transduced by death domain containing adapter

molecules and members of the caspase family of proteases.

These death signals finally cause the degradation of

chromosomal DNA by activated DNase. A human 45 kDa

DNA fragmentation factor (DFF45) was identified recently

which was cleaved by caspase-3 during apoptosis (1).

Mouse homologue of human DFF45 was identified as a

DNase inhibitor designated ICAD (2,3). Upon cleavage of

DFF45/ICAD, a caspase activated deoxyribonuclease

(DFF40/CAD) is released and activated and eventually

causes the degradation of DNA in the nuclei (2-5).

Therefore, the cleavage of DFF45/ICAD, which causes

DFF40/CAD activation and DNA degradation, is the hallmark

of apoptotic cell death.

SOURCE:

Rabbit anti-DFF45 (CT) polyclonal antibody was raised


of human DFF45 (1).

APPLICATION:

This polyclonal antibody can be used for detection of DFF45

and one of the cleaved fragments of DFF45 by Western blot

(6) at 1:1000 to 1:2000 dilution and for immunoprecipitation.

HeLa whole cell lysate can be used as positive control and a

45 kDa band can be detected in non-apoptotic cells. For

research use only.

STORAGE:



year.


DFF45 in HeLa (H), Jurkat

(J), A431 (A), and K562 (K)

whole cell lysate with anti-

DFF45 (CT) at 1:1000

dilution.

REFERENCES:

1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein

that functions downstream of caspase-3 to trigger DNA

fragmentation during apoptosis. Cell 1997;89:175-184

2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,

Nagata S. A caspase-activated DNase that degrades DNA during

apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50

3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in

CAD activation and DNA degradation during apoptosis. Nature

1998;391:96-99

4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The

40-kDa subunit of DNA fragmentation factor induces DNA

fragmentation and chromatin condensation during apoptosis. Proc

Natl Acad Sci USA 1998;95:8461-6

5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,

Nagata S. Molecular cloning and characterization of human

caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8

6. Tang D, Kidd VJ. Cleavage of DFF-45/ICAD by multiple

caspases is essential for its function during apoptosis. J Biol Chem

1998;273:28549-52

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DFF45/35 (NT)

CATALOG No.: 1148 BACKGROUND: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively, (2,4). Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2-5). Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death. SOURCE:

Rabbit anti-DFF45 (NT) polyclonal antibody was raised against a peptide corresponding to amino acids 2 to 21 of human DFF45 (1). APPLICATION: This polyclonal antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution and for immunohistochemistry. Jurkat whole cell lysate can be used as positive control and 45 and 35 kDa bands can be detected in non-apoptotic cells. For research use only.

STORAGE:

It is supplied as purified IgG, 100 µg in 200 µl of PBS containing 0.02% sodium azide. Store at 4°C, stable for one year.


DFF45/35 in HeLa, (H),

K562 (K), Jurkat (J), and

Raji (R) whole cell lysate

with anti-DFF45/35 (NT)

at 1:1000 dilution.

REFERENCES:

1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric

protein that functions downstream of caspase-3 to trigger DNA







1998;391:96-99

4. Gu J, Dong RP, Zhang C, McLaughlin DF, Wu MX, Schlossman

SF. Functional interaction of DFF35 and DFF45 with caspase-

activated DNA fragmentation nuclease DFF40. J Biol Chem

1999;274:20759-62

5. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The





Nagata S. Molecular cloning + characterization of human caspase-

activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-MADD DENN

CATALOG No.: 1150

BACKGROUND:

MAP kinase-activating death domain protein (MADD) was

initially identified as the type 1 tumor necrosis factor receptor

(TNFR1) associated protein though their death domains (1).

Overexpression of MADD activates MAP kinases ERK and

JNK and induces the phosphorylation of cytosolic

phospholipase A2 (1). MADD shares 98% identity with

DENN (for differentially expressed in neoplastic vs. normal

cells), which was recently identified as a substrate for c-jun

N-terminal kinase 3 (JNK3) (2, 3). MADD has greater than

94% overall identity to a GDP/GTP exchange protein Rab3-

GEP (4, 5). MADD is 87% identical to KIAA0358, a brain

protein of unknown function (4). Identification of MADD as a

component of the TNFR1 signaling complex and the

similarity between MADD and Rab3-GEP provides a

connection between TNFR1 activation and downstream

MAP kinase activity through a guanine-nucleotide exchange

protein.

SOURCE:

Rabbit anti-MADD polyclonal antibody was raised against a

peptide corresponding to amino acids 1570 to 1588 of

human MADD (1). The sequence of immunogenic peptide is

identical to that of DENN and differs by one amino acid with

rat GDP/GTP exchange protein Rab3-GEP (2,5).

APPLICATION:

This polyclonal antibody can be used for detection of MADD


from HeLa or NIH3T3 cells can be used as positive control

and 200 to 220 kDa bands should be detected. It is human

and mouse reactive. For research use only.

STORAGE:



one year at 2-8°C.


of MADD in whole cell

lysates from the

indicated cell lines with

anti-MADD at 1:250

dilution.

REFERENCES:

1. Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel

death domain protein that interacts with the type 1 tumor necrosis

factor receptor and activates mitogen-activated protein kinase. J

Biol Chem 1997;272:12069-12075

2. Chow VT, Lee SS. DENN, a novel human gene differentially

expressed in normal and neoplastic cells. DNA Seq 1996;6:263-

273

3. Zhang Y, Zhou L, Miller CA. A splicing variant of a death

domain protein that is regulated by a mitogen-activated kinase is a

substrate for c-Jun N-terminal kinase in the human central nervous

system. Proc Natl Acad Sci U S A 1998;95:2586-2591

4. Brown TL and Howe PH. MADD is highly homologous to a Rab3

guanine-nucleotide exchange protein (Rab3-GEP). Curr Biol

1998;8:R191

5. Wada M, Nakanishi H, Satoh A, Hirano H, Obaishi H, Matsuura

Y, Takai Y. Isolation and characterization of a GDP/GTP exchange

protein specific for the Rab3 subfamily small G proteins. J Biol

Chem 1997;272:3875-3878.

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ASK1 (CT) MAPKKK5

CATALOG No.: 1151

BACKGROUND:

Mitogen-activated protein (MAP) kinase cascades are

activated in response to various extracellular stimuli,

including cytokines, growth factors and environmental

stresses. A novel MAP kinase kinase kinase (MAPKKK) was

recently identified and designated ASK1 (for apoptosis

signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1

activated two different subgroups of MAPKK, MKK4 and

MKK6, which in turn activated c-Jun N-terminal kinase (JNK)

and p38 MAP kinase, respectively. ASK1/MAPKKK5 is

activated by TNFR and Fas through the interaction with

members of the TRAF family and Fas-associated protein

Daxx. Overexpression of ASK1 induced apoptotic cell death,

and a catalytically inactive form of ASK1 inhibited TNF-α-

induced apoptosis. ASK1 is expressed in variety of human

and mouse tissues.

SOURCE:

Rabbit anti-ASK1 polyclonal antibody was raised against a

peptide corresponding to amino acids 1356 to 1375 of

human ASK1 (1). This sequence is different from that of

mouse by last two amino acids (3).

APPLICATION:

This polyclonal antibody can be used for detection of ASK1


from SW1353 can be used as positive control and a 155

kDa band can be detected. For research use only.

STORAGE:


µl of PBS containing 0.02% sodium azide. Store at 4°C,

stable for one year


ASK1 in SW1353 whole cell

lysate with anti-ASK1 at

1:500 dilution.

REFERENCES:

1. Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T,

Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of

apoptosis by ASK1, a mammalian MAPKKK that activates

SAPK/JNK and p38 signaling pathways. Science 1997;275:90-4

2. Wang XS, Diener K, Jannuzzi D, Trollinger D, Tan TH,

Lichenstein H, Zukowski M, Yao Z. Molecular cloning and

characterization of a novel protein kinase with a catalytic domain

homologous to mitogen-activated protein kinase kinase kinase. J

Biol Chem 1996;271:31607-11

3. Tobiume K, Inage T, Takeda K, Enomoto S, Miyazono K, Ichijo

H. Molecular cloning and characterization of the mouse apoptosis

signal-regulating kinase 1. Biochem Biophys Res Commun

1997;239:905-10

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-mFLIP (CT) CASHα

CATALOG No.: 1156

BACKGROUND:



signals are transduced by death domain (DD) containing


family. Caspases-8 (FLICE) and -10 (FLICE2) are two

pivotal members in the ICE/CED-3 protease family. FLICE-

inhibitory proteins were identified in virus and human and

designated v-FLIPs and FLIP respectively (1,2). The human

FLIP was also cloned by several labs independently and

termed Casper, I-FLICE, FLAME-1, CASH, CLARP and

MRIT (3-8). FLIP contains two death effector domains

(DEDs) and a caspase-like domain. FLIP interacts with

adapter protein FADD and caspase-8 and –10, and potently

inhibits apoptosis induced by all known death receptors

CD95, DR3, TRAIL-R and TNFR1.

SOURCE:

Rabbit anti-mFLIP polyclonal antibody was raised against a

peptide corresponding to amino acids 449 to 465 of mouse

FLIPL/CASHα (2,6).

APPLICATION:

This polyclonal antibody can be used for the detection of

FLIP by Western blot at 1:500 to 1:1000 dilution. NIH/3T3


kDa band can be detected. FLIP has short form (FLIPS) and

long form (FLIPL) and this antibody recognizes the FLIP L

only. This antibody is for research use only.

STORAGE:



one year at 2-8°C.

Western blot analysis of mFLIP

in NIH/3T3 whole cell lysate

with anti-mFLIP (CT) at 1:500

dilution. REFERENCES: 1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521. 2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195. 3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763. 4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257. 5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang Y, Fernandes -Alnemri T, Croce CM, Litwack G, Tomaselli KJ, Armstrong RC, Alnemri ES. FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. J Biol Chem 1997;272:18542-18545. 6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM, Wallach D. CASH, a novel caspase homologue with death effector domains. J Biol Chem 1997;272:19641-19644. 7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722. 8. Han DK, Chaudhary PM, Wright ME, Friedman C, Trask BJ, Riedel RT, Baskin DG, Schwartz SM, Hood L. MRIT, a novel death-effector domain-containing protein, interacts with caspases and BclXL and initiates cell death. Proc Natl Acad Sci USA 1997;94:11333-11338. 9. Wallach D. Apoptosis. Placing death under control. Nature 1997;388:123. (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR3 (ED) Wsl-1, Apo-3, TRAMP, LARD

CATALOG No.: 1158

BACKGROUND:





death domain containing receptors, TNFR1 and Fas. A

novel cell death receptor was recently identified by several

groups independently and designated DR3, Wsl-1, Apo-3,

TRAMP and LARD1-5. The ligand for this novel death

receptor has been defined as TWEAK, also termed Apo3L.

DR3 is highly expressed in the tissues enriched in

lymphocytes including PBL, thymus and spleen. Like

TNFR1, DR3 induces apoptosis and NF-κB activation.

SOURCE:

Rabbit anti-DR3 (ED) polyclonal antibody was raised against

a peptide corresponding to amino acids 59 to 77 in

extracellular domain (ED) of human DR3 precusor1,2.

APPLICATION:



Jurkat total cell lysate can be used as positive control and a

59 kDa band should be detected. It is human and mouse


STORAGE:



year.


in Jurkat total cell lysate with

anti-DR3 (ED) at 1:1000

dilution.

REFERENCES:







4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V; Thome

M; Bornand T; Hahne M; Schroter M; Becker K; et al. Immunity,

1997;6:79-88.



(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-FLIP (NT) Casper, I-FLICE, FLAME-1, CASH, CLARP

CATALOG No.: 1159

BACKGROUND:








designated v-FLIPs and FLIP, respectively1,2. The human


termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7.

FLIP contains two death effector domains (DEDs) and a

caspase-like domain. FLIP interacts with adapter protein

FADD and caspase-8 and –10, and potently inhibits

apoptosis induced by all known death receptors. Four splice

variants of c-FLIPs have been identified and termed FLIPα ,

β, γ, and δ, respectively8.

SOURCE:

Rabbit anti-FLIP (NT) polyclonal antibody was raised


human FLIP 2. The sequence is identical in all FLIP splice

variants.

APPLICATION:


1:500 to 1:1000 dilution. It recognizes all FLIP splice

variants including FLIPα, β, γ, and δ and is human, mouse

and rat reactive. For research use only.

STORAGE:



stable for one year.

REFERENCES: 1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E,

Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C,

Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins

(FLIPs) prevent apoptosis induced by death receptors. Nature

1997;386:517-521

2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner

V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D,

French LE, Tschopp J. Inhibition of death receptor signals by

cellular FLIP. Nature 1997;388:190-195

3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and

caspase-related inducer of apoptosis. Immunity 1997;6:751-763

4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel

inhibitor of tumor necrosis factor receptor-1- and CD-95-induced

apoptosis. J Biol Chem 1997;272:17255-17257

5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang

Y, Fernandes-Alnemri T, Croce CM, Litwack G, Tomaselli KJ,

Armstrong RC, Alnemri ES. FLAME-1, a novel FADD-like anti-

apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. J

Biol Chem 1997;272:18542-18545

6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE,

Brodianskii VM, Wallach D. CASH, a novel caspase homologue

with death effector domains. J Biol Chem 1997;272:19641-19644

7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death

effector domain-containing protein interacts with caspase-8 and

regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722

8. Wallach D. Apoptosis. Placing death under control. Nature

1997;388:123 (RD1299).


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-FLIPα (CT) Casper, I-FLICE, FLAME-1, CASH, CLARP

CATALOG No.: 1161

BACKGROUND:








designated v-FLIPs and FLIP, respectively1,2. The human


termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7.

FLIP contains two death effector domains (DEDs) and a

caspase-like domain. FLIP interacts with adapter protein

FADD and caspase-8 and –10, and potently inhibits

apoptosis induced by all known death receptors. Four splice

variants of c-FLIPs have been identified and termed FLIPα ,

β, γ, and δ, respectively8.

SOURCE:

Rabbit anti-FLIP (CT) polyclonal antibody was raised against

a peptide corresponding to amino acids 447 to 464 at C-

terminus of human FLIPα/FLIPL form2.

APPLICATION:

This polyclonal antibody can be used for detection of FLIP

by Western blot at 1:500 to 1:2000 dilution. HeLa whole cell

lysate can be used as positive control and a 55 kDa band

can be detected. It is human, mouse and rat reactive. This

antibody recognizes the FLIPα only. For research use only.

STORAGE:



year.


FLIP in HeLa (H), Jurkat (J),

and K562 (K) whole cell

lysate with anti-FLIP (CT) at

1:1000 dilution.

REFERENCES: 1. Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory

proteins (FLIPs) prevent apoptosis induced by death receptors.

Nature 1997;386:517-521 2. Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor

signals by cellular FLIP. Nature 1997;388:190-195

3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and

caspase-related inducer of apoptosis. Immunity 1997;6:751-763

4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel

inhibitor of tumor necrosis factor receptor-1- and CD-95-induced


5. Srinivasula SM, Ahmad M, Ottilie S, et al.. FLAME-1, a novel

FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-

induced apoptosis. J Biol Chem 1997;272:18542-18545

6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE,

Brodianskii VM, Wallach D. CASH, a novel caspase homologue

with death effector domains. J Biol Chem 1997;272:19641-19644

7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death

effector domain-containing protein interacts with caspase-8 and

regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722

8. Wallach D. Apoptosis. Placing death under control. Nature

1997;388:123 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Daxx (CT)

CATALOG No.: 1163

BACKGROUND:





death domain containing receptors, TNFR1 and Fas. Cell

death signals are transduced by death domain (DD)-

containing adapter molecules and members of the ICE/CED-

3 protease family. A novel DD-containing molecule was

recently cloned from mouse, human and monkey and

designated Daxx1,2. Daxx binds specifically to the Fas death

domain and enhances Fas induced apoptosis and activates

the Jun N-terminal kinase (JNK) pathway. Daxx is widely

expressed in fetal and adult human and mouse tissues

indicating its important function in Fas signaling pathways1,2.

SOURCE:

Rabbit anti-Daxx (CT) polyclonal antibody was raised


at C-terminus of human Daxx 1,2.

STORAGE:



year.

APPLICATION:

This polyclonal antibody can be used for detection of Daxx

by Western blot at 1:500 to 1:1000 dilution. HeLa whole cell

lysate can be used as positive control and a 120 kDa major

band can be detected. The sequences of immunogenic

peptide are identical among human, monkey and mouse

origins of Daxx1,2, and the antibody recognizes Daxx from

all these species. For research use only.

Western blot analysis of Daxx in

HeLa total cell lysate with anti-

Daxx (CT) at 1:1000 dilution.

REFERENCES: 1. Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel

Fas-binding protein that activates JNK and apoptosis. Cell

1997;89:1067-1076

2. Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd.

Cloning and expression of primate Daxx cDNAs and mapping of

the human gene to chromosome 6p21.3 in the MHC region. DNA

Cell Biol 1997;16:1289-1298

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR3 (NT) Wsl-1, Apo-3, TRAMP, LARD

CATALOG No.: 1166

BACKGROUND:






cell death receptor was recently identified by several groups

independently and designated DR3, Wsl-1, Apo-3, TRAMP

and LARD1-5. The ligand for this novel death receptor has

been defined as TWEAK, also termed Apo3L. DR3 is highly

expressed in the tissues enriched in lymphocytes including

PBL, thymus and spleen. Like TNFR1, DR3 induces

apoptosis and NF-κB activation.

SOURCE:

Rabbit anti-DR3 (NT) polyclonal antibody was raised against


DR3 precusor1,2.

APPLICATION:

This polyclonal antibody can be used for detection of DR3 by

Western blot at 1:500 to 1:1000 dilution. Jurkat whole cell


can be detected. It is human and mouse reactive and has no

cross reaction to other death receptors. For research use

only.

STORAGE: It is supplied as purified IgG, 100 µg in 200 µl


for one year.


of DR3 in Jurkat total


DR3 (NT) at 1:1000

dilution.

REFERENCES:







4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V;

Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al.

Immunity, 1997;6:79-88.



(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR4 (NT) TRAIL-R1

CATALOG No.: 1167

BACKGROUND:






death domain containing receptor was recently identified and

designated DR4 (for death receptor 4)1. The ligand for this

novel death receptor has been identified and termed

TRAIL2,3, which is a new member in the TNF family. DR4 is

also called TRAIL receptor-1 (TRAIL-R1)4. DR4 is expressed

in most of human tissues including spleen, peripheral blood

leukocytes, small intestine and thymus. Like TNFR1, Fas

and DR3, DR4 mediates apoptosis and NF-κB activation in

many tissues and cells.

SOURCE:


a peptide corresponding to amino acid 1 to 20 of human

DR4 mature protein.

APPLICATION:


Western blot at 1:500 to 1:1000 dilution. HeLa or Jurkat



STORAGE:

Supplied as purified IgG, 100 µg in 200 µl of PBS


year.


in HeLa (H), K562 (K), and

Jurkat (J) whole cell lysate

with anti-DR4 (NT) at 1:500

dilution.

REFERENCES: 1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J;

Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science;

1997;276:111-113

2. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK,

Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and

characterization of a new member of the TNF family that induces apoptosis.

Immunity 1995;3:673-682

3. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A.

Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis

factor cytokine family. J. Biol. Chem. 1996;271:12687-90

4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T,

Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-

dependent apoptosis and activate NF-κB. Immunity 1997;7:831-836

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bonzo (CT) STRL33, TYMSTR

CATALOG NO.: 1170

BACKGROUND:

Human immunodeficiency virus (HIV) and simian

immunodeficiency virus (SIV) require coreceptors, in addition

to CD4, to infect target cells. Some G protein-coupled

receptors including CCR5, CXCR4, CCR3, and CCR2b in

the chemokine receptor family have been identified as HIV

coreceptors. An orphan G protein-coupled receptor was

recently cloned and designated Bonzo, STRL33 and

TYMSTR, and identified as HIV and SIV coreceptor (1-4).

Bonzo/STRL33 is used by SIV, HIV-2 and HIV-1. The

messenger RNA of Bonzo/STRL33 is expressed in lymphoid

tissues and activated peripheral blood lymphocytes.

SOURCE:

Rabbit anti-Bonzo polyclonal antibody was raised against a


origin (1,2). The sequence of this peptide is identical to those

of macaque and African green monkey.

APPLICATION:

This polyclonal antibody can be used for detection of Bonzo

by Western blot at 1:1000 dilution. Whole cell lysate from

SW1353 cells can be used as positive control. This antibody


STORAGE:



for one year.


Bonzo in SW1353 total

cells lysate with anti-

Bonzo (CT) at 1:1000

dilution.

REFERENCES:

1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR.

Expression cloning of new receptors used by simian and human

immunodeficiency viruses. Nature 1997;388:296-300

2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber

JM. STRL33, A novel chemokine receptor-like protein, functions as

a fusion cofactor for both macrophage-tropic and T cell line-tropic

HIV-1. J Exp Med 1997;185:2015-23

3. Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new

SIV co-receptor, STRL33. Nature 1997;388:238

4. Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher

P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M,

Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine

receptor selectively expressed in activated T cells, exhibits HIV -1

coreceptor function. Curr Biol 1997;7:652-60 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ICAD (NT)

CATALOG No.: 2001

BACKGROUND:






chromosomal DNA by activated DNase. A human DNA

fragmentation factor (DFF) was identified recently which is

cleaved by caspase-3 during apoptosis (1). Mouse

homologue of human DFF was identified as a DNase

inhibitor designated ICAD, for inhibitor of caspase-activated

DNase (2,3). Upon cleavage of DFF/ICAD, a caspase

activated deoxyribonuclease (CAD) is released and

activated and eventually causes the degradation of DNA in

the nuclei (2,3). Therefore, the cleavage of CAD inhibitor

molecule DFF/ICAD, which causes DNase activation and

DNA degradation, is the hallmark of apoptotic cell death (4).

SOURCE:

Rabbit anti-ICAD (NT) polyclonal antibody was raised


mouse ICAD (2).

APPLICATION:

This polyclonal antibody can be used for detection of of

ICAD by Western blot at 1:500 to 1:1000 dilution. Murine

lung tissue lysate can be used as positive control and a 45

kDa band cad be detected. This antibody is for research use

only.

STORAGE:





ICAD in murine lung

tissue lysate with anti-

ICAD (NT) at 1:500

dilution.

REFERENCES:









1998;391:96-99

4. Wyllie A. Apoptosis. An endonuclease at last. Nature

1998;39120-21(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ICAD (CT)

CATALOG No.: 2003

BACKGROUND:






chromosomal DNA by activated DNase. A human DNA

fragmentation factor (DFF) was identified recently which was

cleaved by caspase-3 during apoptosis (1). Mouse

homologue of human DFF was identified as a DNase

inhibitor designated ICAD, for inhibitor of caspase-activated

DNase (2,3). Upon cleavage of DFF/ICAD, a caspase

activated deoxyribonuclease (CAD) is released and

activated and eventually causes the degradation of DNA in

the nuclei (2,3). Therefore, the cleavage of CAD inhibitor

molecule DFF/ICAD, which causes DNase activation and

DNA degradation, is the hallmark of apoptotic cell death (4).

SOURCE:

Rabbit anti-ICAD (CT) polyclonal antibody was raised


of mouse ICAD (2).

APPLICATION:

This polyclonal antibody can be used for detection of ICAD

by Western blot at 1:500 to 1:2000 dilution. Murine lung

tissue lysate can be used as positive control and a 45 kDa

band can be detected. This antibody is for research use

only.

STORAGE:





of ICAD in murine lung

(L), brain (B), liver (L),

and kidney tissue

lysate with anti-ICAD

(CT) at 1:1000 dilution.

REFERENCES:









1998;391:96-99

4. Wyllie A. Apoptosis. An endonuclease at last. Nature

1998;39120-21

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CAD (I17)

CATALOG No.: 2007

BACKGROUND:






chromosomal DNA by activated DNase. A mouse DNase

that causes DNA fragmentation was identified recently and

designated CAD (for caspase activated deoxyribonuclease)

(1,2). The human homologue of mouse CAD was more

recently identified by two groups independently and termed

CPAN and DFF40 (3,4). Human DFF45 and its mouse

homologue ICAD are the inhibitors of CPAN/DFF40 and

CAD, respectively (5). Upon cleavage of DFF45/ICAD by

activated caspase, DFF40/CAD is released and activated

and eventually causes the degradation of DNA in the nuclei

(1-4). Activation of CAD/DFF40, which causes DNA

degradation, is the hallmark of apoptotic cell death.

SOURCE:

Rabbit anti-CAD (I17) polyclonal antibody was raised against

a peptide corresponding to amino acids 205 to 222 of murine

CAD (1).

APPLICATION:

This polyclonal antibody can be used for detection of CAD

by Western blot at 1:500 to 1:1000 dilution. Murine kidney


band should be detected. It is mouse and rat reactive. For

research use only.

STORAGE:





CAD in murine kidney

tissue lysate with anti-CAD

(I17) at 1:500 dilution.

REFERENCES:






1998;391:96-99

3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The




4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,

Williams LT. CPAN, a human nuclease regulated by the caspase-

sensitive inhibitor DFF45. Curr Biol 1998;8:537-40




(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CAD (CT)

CATALOG No.: 2011

BACKGROUND:










recently identified by two groups independently and termed

CPAN and DFF40 (3,4). Human DFF45 and its mouse

homologue ICAD are the inhibitors of CPAN/DFF40 and

CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD

by activated caspase, DFF40/CAD is released and activated

and eventually causes the degradation of DNA in the nuclei

(1-4). Activation of CAD/DFF40, which causes DNA


SOURCE:

Rabbit anti-CAD (CT) polyclonal antibody was raised against


CAD (1).

APPLICATION:

This polyclonal antibody can be used for detection of CAD

by Western blot at 1:500 to 1:1000 dilution. Tissue lysate

from murine lung or kidney can be used as positive control

and a 40 kDa band should be detected. For research use

only.

STORAGE:





CAD in murine lung (L) and

kidney (K) tissue lysates

with anti-CAD (CT) at 1:500

dilution.

REFERENCES:






1998;391:96-99











(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Apaf-1 (NT)

CATALOG No.: 2013

BACKGROUND:





The mammalian homologues of the key cell death gene

CED-4 in C. elegans has been identified recently from

human and mouse and designated Apaf-1 (for apoptosis

protease-activating factor 1) (1,2). Apaf-1 binds to

cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads

to caspase-9 activation. Activated caspase-9 in turn cleaves

and activates caspase-3 that is one of the key proteases,

being responsible for the proteolytic cleavage of many key

proteins in apoptosis (3). Apaf-1 can also associate with

caspase-4 and caspase-8 (4). Apaf-1 is ubiquitously

expressed in human tissues (1).

SOURCE:

Rabbit anti-Apaf-1 (NT) polyclonal antibody was raised


human Apaf-1 (1). The sequences of the immunogenic

peptide are identical between human and mouse (1,2)

APPLICATION:

This polyclonal antibody can be used for detection of Apaf-1

by Western blot at 1:1000 to 1:2000 dilution. Whole cell

lysate from HeLa cells can be used as positive control and a

130 kDa band should be detected. It is human, mouse, and

rat reactive. This antibody is for research use only.

STORAGE:



azide. Store at 4°C, stable for one year.

REFERENCES: 1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human

protein homologous to C. elegans CED-4, participates in

cytochrome c-dependent activation of caspase-3. Cell

1997;90:405-13

2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P.

Apaf1 (CED-4 homolog) regulates programmed cell death in

mammalian development. Cell 1998;94:727-37

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M,

Alnemri ES, Wang X. Cytochrome c and dATP-dependent

formation ofApaf-1/caspase-9 complex initiates an apoptotic

protease cascade. Cell 1997;91:479-89

4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts

with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.

Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Apaf1 (CT)

CATALOG No.: 2015

BACKGROUND:





The mammalian homologous of the key cell death gene

CED-4 in C. elegans was identified recently from human and

mouse and designated Apaf1 for apoptosis protease-

activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2)

and caspase-9 (Apaf3), which leads to caspase-9 activation.

Activated caspase-9 in turn cleaves and activates caspase-3

that is one of the key proteases, being responsible for the

proteolytic cleavage of many key proteins in apoptosis (3).

Apaf1 can also associate with caspase-4 and caspase-8 (4).

Apaf1 transcript is ubiquitously expressed in human tissues

(1).

SOURCE:

Rabbit anti-Apaf1 (CT) polyclonal antibody was raised

against a peptide corresponding to amino acids 1158 to

1177 of human Apaf1 (1). The sequence of the immunogenic

peptide differs from that of murine Apaf1 by one amino acid

(1,2)

APPLICATION:

This polyclonal antibody can be used for detection of Apaf1

by Western blot at 1:500 to 1:2000 dilution. Human heart


band should be detected. It is human, mouse and rat


RELATED PRODUCTS:

Blocking peptide, 50 µg / 250 µl, is available for competition


STORAGE:





Apaf1 in human heart

tissue lysate with anti-Apaf1 (2015) at 1:500 dilution in

the absence (A) or

presence (B) of blocking

peptide.

REFERENCES:

1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human

protein homologous to C. elegans CED-4, participates in

cytochrome c-dependent activation of caspase-3. Cell

1997;90:405-13

2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P.

Apaf1 (CED-4 homolog) regulates programmed cell death in

mammalian development. Cell 1998;94:727-37



formation of Apaf-1/caspase-9 complex initiates an apoptotic


4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts

with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.

Proc Natl Acad Sci USA 1998;95:4386-91

(WD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR5 Apo2, TRAIL-R2, TRICK2, KILLER

CATALOG No.: 2019

BACKGROUND:

Apoptosis is induced by certain cytokines including TNF and

Fas ligand in the TNF family through their death domain

containing receptors. TRAIL/Apo2L is a new member of the

TNF family. DR4 was recently identified as the receptor for

TRAIL. A novel death domain containing receptor for TRAIL

was more recently identified and designated DR5, Apo2,

TRAIL-R2, TRICK2, or KILLER by several groups

independently (1-7). Like DR4, DR5 transcript is widely

expressed in normal tissues and in many types of tumor

cells. DR5 binds to TRAIL and mediates TRAIL induced cell

death. Overexpression of DR5 induces apoptosis and

activates NF-κB.

SOURCE:

Rabbit anti-DR5 polyclonal antibody was raised against a


DR5 precursor (1,2).

APPLICATION:



Whole cell lysate from HeLa cells can be used as positive

control. It is human and mouse reactive and has no cross

reaction to DR4. For research use only.

STORAGE:

It is supplied as affinity chromatography purified IgG, 100 µg

in 200 µl of PBS containing 0.02% sodium azide. Store at -

20°C. Stable for one year at 2-8°C.

REFERENCES:



Science 1997;277:815-8


in HeLa (H) and K562 (K)

whole cell lysates with anti-

DR5 at 1:500 dilution.





1997;277:818-21

3. Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh

JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA,

Goodwin RG, Rauch CT. TRAIL-R2: a novel apoptosis-mediating

receptor for TRAIL. EMBO J 1997;16:5386-97

4. MacFarlane M, Ahmad M, Srinivasula SM, Fernandes-Alnemri

T, Cohen GM, Alnemri ES. Identification and molecular cloning of

two novel receptors for the cytotoxic ligand TRA IL. J Biol Chem

1997;272:25417-20

5. Screaton GR, Mongkolsapaya J, Xu XN, Cowper AE, McMichael

AJ, Bell JI. TRICK2, a new alternatively spliced receptor that

transduces the cytotoxic signal from TRAIL. Curr Biol 1997;7:693-6

6. Wu GS, Burns TF, McDonald ER 3rd, Jiang W, Meng R, Krantz

ID, Kao G, Gan DD, Zhou JY, Muschel R, Hamilton SR, Spinner

NB, Markowitz S, Wu G, el-Deiry WS. KILLER/DR5 is a DNA

damage-inducible p53-regulated death receptor gene. Nat Genet

1997;17:141-3

7. Chaudhary PM, Eby M, Jasmin A, Bookwalter A, Murray J, Hood

L Death receptor 5, a new member of the TNFR family, and DR4

induce FADD-dependent apoptosis and activate the NF-kappaB

pathway. Immunity 1997;7:821-30 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DcR2 (ID) TRAIL-R4, TRUNDD

CATALOG No.: 2021

BACKGROUND:




TNF family and induces apoptosis of a variety of tumor cell

lines. DR4 and DR5 are the recently identified functional

receptors for TRAIL, and DcR1/TRID is a decoy receptor (1-

3). Another member of the TRAIL receptor family was more

recently identified and designated DcR2, TRAIL-R4, or

TRUNDD (4-6). DcR2 has an extracellular TRAIL-binding

domain but lacks intracellular death domain and does not

induce apoptosis. Like DR4 and DR5, DcR2 transcript is

widely expressed in normal human tissues. Overexpression

of DcR2 attenuated TRAIL-induced apoptosis.

SOURCE:

Rabbit anti-DcR2 (ID) polyclonal antibody was raised against


DcR2 precursor (4-6).

APPLICATION:

This polyclonal antibody can be used for detection of DcR2



control. It is human, mouse, and rat reactive. For research

use only.

STORAGE:


in 200 µl of PBS containing 0.02% sodium azide. Store at -

20°C. Stable for one year at 2-8°C.


DcR2 in HeLa whole cell

lysate with anti-DcR2 (ID)

at 1:1000 dilution.

REFERENCES: 1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R;

Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand

TRAIL. Science; 1997;276:111-113



Science 1997;277:815-8





1997;277:818-21

4. Marsters SA, Sheridan JP, Pitti RM, Huang A, Skubatch M,

Baldwin D, Yuan J, Gurney A, Goddard AD, Godowski P,

Ashkenazi A. A novel receptor for Apo2L/TRAIL contains a

truncated death domain. Curr Biol 1997;7:1003-6

5. Degli-Esposti MA, Dougall WC, Smolak PJ, Waugh JY, Smith

CA, Goodwin RG. The novel receptor TRAIL-R4 induces NF-

kappaB and protects against TRAIL-mediated apoptosis, yet

retains an incomplete death domain. Immunity 1997;7:813-20

6. Pan G, Ni J, Yu G, Wei YF, Dixit VM. TRUNDD, a new member

of the TRAIL receptor family that antagonizes TRAIL signalling.

FEBS Lett 1998;424:41-5

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKα (C1)

CATALOG No.: 2025

BACKGROUND:





response to extracellular stimuli including IL-1, TNFα, and

bacteria product LPS. NF-κB is associated with IκB proteins

in the cell cytoplasm, which inhibit NF-κB activity. The long-

sought IκB kinase (IKK), which phosphorylates IκB, and

mediates IκB degradation and NF-κB activation, was

recently identified by several laboratories (1-5). IKK is a

serine protein kinase, and the IKK complex contains alpha

and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact

with each other and both are essential for the NF-κB

activation. IKKα specifically phosphorylates IκB-α. IKKα is

expressed in variety of human tissues.

SOURCE:

Rabbit anti-IKKα (C1) polyclonal antibody was raised against


IKKα (1,2), which differs from corresponding murine

sequence by four amino acids.

APPLICATION:

This polyclonal antibody can be used for detection of IKKα

by Western blot at 1:1000 to 1:2000 dilution. Whole cell

lysate from HeLa or Jurkat cells can be used as positive

control and an 85 kDa band should be detected. It has no

cross response to IKKβ or IKKγ. For research use only.

STORAGE:




Western blot analysis of IKKα

in HeLa whole cell lysate with

anti-IKKα (C1) at 1:1000

dilution.

REFERENCES:

1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A

cytokine-responsive IkappaB kinase that activates the transcription

factor NF-kappaB. Nature 1997;388:548-54



1997;90:373-83

3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The

IkappaB kinase complex (IKK) contains two kinase subunits,

IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and

NF-kappaB activation. Cell 1997;91:243-52

4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB



5. Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J,

Young DB, Barbosa M, Mann M, Manning A, Rao A. IKK-1 and

IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB

activation. Science 1997; 278:860-6 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ADAM10 (CT) KUZ, MADM

CATALOG No.: 2051 BACKGROUND: Proinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4). SOURCE: Rabbit anti-ADAM10 (CT) polyclonal antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10 (1). This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4). APPLICATION: This polyclonal antibody can be used for detection of ADAM10 by Western blot at 1:500 to 1:2000 dilution. Jurkat whole cell lysate can be used as positive control and an approximately 85 kDa band can be detected, which may represent precursor. A 60 kDa faint band was detected in some cell lines including Jurkat, which appears to be the processed mature protein. For research use only.

STORAGE:

It is supplied as immunoaffinity chromatography purified IgG, 100 µg in 200 µl of PBS containing 0.02% sodium azide. Store at -20°C, stable for one year.


ADAM10 in Jurkat whole cell

lysate in the absence (1) or

presence (2) of blocking

peptide (Catalog no. 2051P)

with anti-ADAM10 (CT) at

1:1000 dilution.

REFERENCES: 1. Rosendahl MS, Ko SC, Long DL, et al. Identification and

characterization of a pro-tumor necrosis factor-alpha-processing

enzyme from the ADAM family of zinc metalloproteases. J Biol

Chem 1997;272:24588-93

2. Pan D, Rubin GM. Kuzbanian controls proteolytic processing of

Notch and mediates lateral inhibition during Drosophila and

vertebrate neurogenesis. Cell 1997;90:271-80

3. Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T,

Artavanis-Tsakonas S. Processing of the notch ligand delta by the

metalloprotease Kuzbanian. Science 1999;283:91-4

4. Howard L, Lu X, Mitchell S, Griffiths S, Glynn P. Molecular

cloning of MADM: a catalytically active mammalian disintegrin-

metalloprotease expressed in various cell types. Biochem J

1996;317:45-50

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-FLIP γ/δ (CT) FLIP, I-FLICE, FLAME-1, CASH, CLARP

CATALOG No.: 2055

BACKGROUND:



signals are transduced by death domain (DD)-containing





designated v-FLIPs and c-FLIPs, respectively1,2. The human

FLIPs were also cloned by several labs independently and

termed Casper, I-FLICE, FLAME-1, CASH, CLARP and

usurpin3-7. FLIP contains two death effector domains (DEDs)

and a caspase-like domain. FLIP interacts with adapter

protein FADD and caspase-8 and –10, and potently inhibits

apoptosis induced by all known death receptors CD95, DR3,

TRAIL-R and TNFR1. Four splice variants of c-FLIPs have

been identified and termed FLIPα, β, γ, and δ, respectively8.

SOURCE:

Rabbit anti-FLIP (CT) polyclonal antibody was raised against

a peptide corresponding to amino acids 191 to 209 at C-

terminus of human FLIPδ/FLIP s form2.

APPLICATION:

This antibody can be used for detection of FLIP by Western

blot at 1:1000 dilution. Jurkat cell lysate can be used as

positive control and 35 kDa and 25 kDa bands can be

detected. This antibody recognizes human and murine FLIPγ

and FLIPδ but not FLIPα and FLIPβ. For research use only.

STORAGE:





FLIPγ/δ in total cell

lysates from HeLa (H),

Jurkat (J), THP-1 (T),

A431 (A), K562 (K) and

NIH3T3 (N) cells with

anti-FLIPγ/δ (CT) at

1:1000 dilution.

REFERENCES: 1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F,

Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH,

Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent

apoptosis induced by death receptors . Nature 1997;386:517-521

2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V,

Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE,

Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature

1997;388:190-195

3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and caspase-related

inducer of apoptosis. Immunity 1997;6:751-763

4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of

tumor necrosis factor receptor-1- and CD-95-induced apoptosis . J Biol Chem

1997;272:17255-17257

5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang Y,

Fernandes -Alnemri T, Croce CM, Litwack G, Tomaselli KJ, Armstrong RC,

Alnemri ES. FLAME-1, a novel FADD-like anti-apoptotic molecule that

regulates Fas/TNFR1-induced apoptosis. J Biol Chem 1997;272:18542-

18545

6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM,

Wallach D. CASH, a novel caspase homologue with death effector domains.

J Biol Chem 1997;272:19641-19644

7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death effector

domain-containing protein interacts with caspase-8 and regulates apoptosis.

Proc Natl Acad Sci USA 1997;94:10717-10722

8. Tschopp J, Irmler M, Thome M. Inhibition of fas death signals by FLIPs.

Curr Opin Immunol 1998;10:552-8 (RD 1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DNase II (CT)

CATALOG No.: 2059

BACKGROUND:

Apoptosis is characterized by several morphological nuclear

changes including chromatin condensation and nuclear

fragmentation. These changes are triggered by the activation

of members of caspase family, caspase activated DNase,

and several novel proteins including AIF and Acinus (1).

DNase II causes both chromatin condensation and DNA

fragmentation (1). The genes encoding human (2-4), porcine

(4), and murine (5) DNase II have been cloned. The DNase

II gene encodes a 40 kDa proenzyme. The mature enzyme

consists of two non-identical subunits, the 32 kDa (α) and 12

kDa (β) chains, generated by proteolytic processing.

Overexpression of DNase II induces chromatin condensation

(3). DNase II is ubiquitously expressed in human tissues.

SOURCE:

Rabbit anti-DNase II (CT) polyclonal antibody was raised


of human DNase II precursor (2-4).

APPLICATION:

This polyclonal antibody can be used for detection of DNase

II expression by Western blot at 1:500 to 1:1000 dilution.

Human spleen tissue lysate or THP-1 cell lysate can be used

as positive control and an approximate 40 kDa band can be

detected, which represents the pro-enzyme of DNase II. For

research use only.

STORAGE:





DNase II in human spleen

tissue lysate in the absence

(lane 1) or presence (lane

2) of blocking peptide with

anti-DNase II (CT) at 1:500

dilution.

REFERENCES: 1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature

1999;401:127-8

2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O,

Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding

human deoxyribonuclease II. J Biol Chem 1998;273:2610-6

3. Krieser RJ, Eastman A. The cloning and expression of human

deoxyribonuclease II. A possible role in apoptosis. J Biol Chem

1998;273:30909-14

4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine

and human DNase II. Biochem Biophys Res Commun

1998;247:864-9

5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular

cloning and characterization of human and murine DNase II. Gene

1998;215:281-9

(RD0400)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bim (IN) BOD

CATALOG No.: 2065

BACKGROUND:

Members in the Bcl-2 family are critical regulators of

apoptosis by either inhibiting or promoting cell death. Bcl-2

homology 3 (BH3) domain is a potent death domain. BH3

domain containing pro-apoptotic proteins, including Bad,

Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2

family. A novel BH3 domain containing protein was recently

identified and designated Bim or BOD in human, mouse and

rat (1,2). Bim/BOD interacts with diverse members in the

pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-

w. Bim/BOD induces apoptosis. The messenger RNA of Bim

is ubiquitously expressed in multiple tissues and cell lines

(1,2).

SOURCE:

Rabbit anti-Bim (IN) polyclonal antibody was raised against a


origin (1). The sequence is identical to that of mouse and

differs from that of rat by one amino acid.

APPLICATION:

This polyclonal antibody can be used for detection of Bim by

Western blot at 1:1000 dilution. Human K562 cell lysate can

be used as positive control and a 23 kDa band can be

detected. This antibody is human, mouse, and rat reactive.

This antibody is for research use only.

STORAGE:




Western blot analysis of Bim in

K562 (K) and A549 (A) whole cell

lysates with anti-Bim (IN) at

1:1000 dilution.

REFERENCES:

1. O'Connor,L., Strasser,A., O'Reilly,L.A., Hausmann,G.,

Adams,J.M., Cory,S. and Huang,D.C. Bim: a novel member of the

Bcl-2 family that promotes apoptosis. EMBO J. 1998;17:384-395

2. Hsu, S.Y., Lin, P., and Hsueh, A.J. BOD (Bcl-2-related ovarian

death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-

2 protein capable of dimerization with diverse antiapoptotic Bcl-2

members. Mol Endocrinol 1998;12:1432-40

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ZIP kinase (IN)

CATALOG No.: 2067

BACKGROUND:

Apoptosis is mediated by death domain containing adapter

molecules and a caspase family of proteases. Certain

serine/threonine protein kinases, such as ASK-1 and RIP,

are mediators of apoptosis. A novel serine/threonine kinase

that mediates apoptosis was recently identified and

designated ZIP kinase (1). ZIP kinase contains an N-terminal

kinase domain and a C-terminal leucine zipper structure and

binds to ATF4 that is a member of ATF/CREB family. ZIP

kinase has high sequence homology to DAP kinase (death-

associated protein kinase), which is a mediator of apoptosis

induced by gamma interferon. Overexpression of ZIP kinase

induces apoptosis. ZIP and DAP kinases represent a novel

kinase family, which mediates apoptosis through their

catalytic activities. The messenger RNA was ubiquitously

expressed in various tissues (1).

SOURCE:

Rabbit anti-ZIP kinase (IN) polyclonal antibody was raised


of human origin (1).

APPLICATION:

This polyclonal antibody can be used for detection of ZIP

kinase by Western blot at 1:1000 dilution. Jurkat or HeLa


kDa band should be detected. It has no cross response to

DAP kinase. It is human, mouse, and rat reactive. For

research use only.

STORAGE:

Supplied as immunoaffinity chromatography purified IgG,

100 µg in 200 µl of PBS containing 0.02% sodium azide.

Store at 4°C, stable for one year.

Western blot analysis of ZIP

kinase in HeLa (H) and Jurkat

(J) whole cell lysates with

anti-ZIP kinase (IN) at 1:500

dilution.

REFERENCES:

1. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase,

a novel serine/threonine kinase which mediates apoptosis. Mol Cell

Biol 1998;18:1642-51

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-9 (I16) ICE-LAP6, Mch6, Apaf-3

CATALOG No.: 2071

BACKGROUND:





A novel member in the caspase family was recently

identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).

Caspase-9 and Apaf-1 bind to each other, which leads to

caspase-9 activation (3). Caspase-9 is also activated by

granzyme B and CPP32 (1,2). Activated caspase-9 cleaves



proteins in apoptosis (3). Caspase-9 play a central role in

cell death induced by a wide variety of apoptosis activators

including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4).

Caspase-9 is expressed in a variety of human tissues (1,2).

SOURCE:

Rabbit anti-caspase-9 (I16) polyclonal antibody was raised


human caspase-9 (1-3).

APPLICATION:




control and a 46 kDa band should be detected. It has no

cross response to other members in caspase family. It is

human, mouse, and rat reactive. This antibody is for

research use only.

STORAGE:





caspase-9 in HeLa whole


caspase-9 (I16) at 1:1000

dilution.

REFERENCES:

1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He

WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene

family, is activated by the cytotoxic T cell protease granzyme B. J

Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N,

Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G,

Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like

homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are

substrates for the apoptotic mediator CPP32. J Biol Chem

1996;271:27099-106





4. Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1

form a ternary complex. J Biol Chem 1998;273:5841-5

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-9 (I20) ICE-LAP6, Mch6, Apaf-3

CATALOG No.: 2073

BACKGROUND:





A novel member in the caspase family was recently

identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).

Caspase-9 and Apaf-1 bind to each other, which leads to

caspase-9 activation (3). Caspase-9 is also activated by

granzyme B and CPP32 (1,2). Activated caspase-9 cleaves



proteins in apoptosis (3). Caspase-9 play a central role in

cell death induced by a wide variety of apoptosis activators

including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4).

Caspase-9 is expressed in a variety of human tissues (1,2).

SOURCE:

Rabbit anti-caspase-9 (I20) polyclonal antibody was raised


of human caspase-9 (1-3).

APPLICATION:




control and a 46 kDa band should be detected. It has no

cross response to other members in caspase family. This

antibody is for research use only.

STORAGE:





caspase-9 in HeLa whole cell

lysate with anti-caspase-9

(I20) at 1:1000 dilution.

REFERENCES:

1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He

WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene

family, is activated by the cytotoxic T cell protease granzyme B. J

Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N,

Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G,

Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like

homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are

substrates for the apoptotic mediator CPP32. J Biol Chem

1996;271:27099-106





4. Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1

form a ternary complex. J Biol Chem 1998;273:5841-5.

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-RICK (NT) RIP2, CARDIAK

CATALOG No.: 2075

BACKGROUND:

Apoptosis is mediated by death domain (DD) and/or caspase

recruitment domain (CARD) containing molecules and a

caspase family of proteases. DD-containing serine/threonine

kinase RIP regulates Fas-induced apoptosis. A novel CARD-

containing serine/threonine kinase was recently identified

and designated RICK/RIP2/CARDIAK for RIP-like interacting

CLARP kinase, receptor interacting protein-2, and CARD-

containing ICE associated kinase, respectively, (1-3). RICK

contains an N-terminal kinase catalytic domain and a C-

terminal CARD domain. Overexpression of RICK induced

apoptosis and activation of NF-κB and JNK. RICK interacts

with members of the TRAF family, CLARP and caspase-1.

Thus, RICK represents a novel kinase that regulates TNF

and Fas induced-apoptosis and that is involved in the

generation of proinflammatory cytokine IL-1β. The

messenger RNA of RICK is expressed in multiple human

tissues (1).

SOURCE:

Rabbit anti-RICK (NT) polyclonal antibody was raised


human origin (1).

APPLICATION:

This polyclonal antibody can be used for detection of RICK

by Western blot at 1:1000 dilution. A431 or K562 whole cell

lysate can be used as positive control and an approximately

60 kDa band can be detected. It is human, mouse, and rat


STORAGE:





RICK in A431 (A) and K562

(K) whole cell lysate with

anti-RICK (NT) at 1:1000

dilution.

REFERENCES:

1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a

novel protein kinase containing a caspase recruitment domain,

interacts with CLARP and regulates CD95-mediated apoptosis. J

Biol Chem 1998;273:12296-300

2. McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-kappaB-

activating and cell death-inducing kinase. J Biol Chem

1998;273:16968-75

3. Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL,

Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like

kinase that associates with caspase-1. Curr Biol 1998;8:885-8

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ARC (CT)

CATALOG No.: 2081

BACKGROUND:

Apoptosis is regulated by death domain (DD) and/or

caspase recruitment domain (CARD) containing molecules

and a caspase family of proteases. CARD domain containing

cell death regulators include RAIDD, Apaf-1, caspase-9, and

caspase-2. A novel CARD domain containing protein was

recently identified and designated ARC for apoptosis

repressor with CARD (1). ARC interacts with caspase-2 and

–8 and inhibits enzymatic activity of caspase-8. ARC

suppresses apoptosis induced by cell death adapters FADD

and TRADD and by cell death receptors Fas, TNFR-1 and

DR3. The messenger RNA of ARC is primarily expressed in

skeletal muscle and cardiac tissue (1).

SOURCE:

Rabbit anti-ARC (CT) polyclonal antibody was raised against


origin (1).

APPLICATION:

This polyclonal antibody can be used for detection of ARC


HeLa cells can be used as positive control and an

approximately 25 kDa band can be detected. This antibody


STORAGE:





ARC in HeLa (H), KB (K),

and A549 (A) whole cell

lysates with anti-ARC (CT)

at 1:1000 dilution.

REFERENCES:

1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of

apoptosis expressed in skeletal muscle and heart that interacts

selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-

60 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CIDE-A (CT)

CATALOG No.: 2085

BACKGROUND:


family of cell death receptors. Cell death signals are

transduced by DD-, DED-, or CARD-containing molecules

and members of the caspase family of proteases. These

death signals finally cause the degradation of chromosomal

DNA by activated DNase DFF40/CAD, which is chaperoned

and inhibited by DFF45/ICAD (1-4). DFF45 related proteins

CIDE-A and CIDE-B (for cell death-inducing DFF-like

effector A and B) were recently identified (5). CIDE contains

a new type of domain termed CIDE-N, which has high

homology with the regulatory domains of DFF45/ICAD and

DFF40/CAD (5,6). Expression of CIDE-A induces DNA

fragmentation and activates apoptosis, which is inhibited by

DFF45. CIDE-A is expressed in many tissues.

SOURCE:

Rabbit anti-CIDE-A (CT) polyclonal antibody was raised


of human CIDE-A (5).

APPLICATION:

This polyclonal antibody can be used for detection of CIDE-A

by Western blot at 1:1000 to 1:2000 dilution. Human brain

tissue lysate can be used as positive control and an

approximately 23 kDa band can be detected. It has no cross

activity to CIDE-B. For research use only.

STORAGE:

It is supplied as immunoaffinity chromatography purified IgG,



RELATED PRODUCTS:


studies (Catalog No. 2085P). Human brain tissue lysate,

200 µl/100 µl, is available for positive control (Catalog No.

1303).


CIDE-A in human brain tissue

lysate in the absence (A) or

presence (B) of peptide

(2085P) with anti-CIDE-A


REFERENCES: 1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric


fragmentation during apoptosis. Cell 1997;89:175-184 2. Enari M,

Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A

caspase-activated DNase that degrades DNA during apoptosis,

and its inhibitor ICAD. Nature 1998;391:43-50



1998;391:96-99





5. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel

family of cell death activators with homology to the 45 kDa subunit

of the DNA fragmentation factor. EMBO J 1998;17:2526-33

6. Lugovskoy AA, Zhou P, Chou JJ, McCarty JS, Li P, Wagner G.

Solution structure of the CIDE-N domain of CIDE -B and a model

for CIDE-N/CIDE -N interactions in the DNA fragmentation pathway

of apoptosis. Cell 1999;99(7):747-55

(WD0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CIDE-A (CT)

CATALOG No.: 2089

BACKGROUND:






chromosomal DNA by activated DNase. DFF45/ICAD has

been identified as inhibitor of caspase activated DNase

DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B

(for cell death-inducing DFF-like effector A and B) were

recently identified (1). CIDE contains a new type of domain

termed CIDE-N, which has high homology with the

regulatory domains of DFF45/ICAD and DFF40/CAD (1,2).

Expression of CIDE-A induces DNA fragmentation and

activates apoptosis, which is inhibited by DFF45. CIDE-A is

a DFF45-inhibitable effector that promotes cell death and

DNA fragmentation. CIDE-A is expressed in many tissues.

SOURCE:

Rabbit anti-CIDE-A (CT) polyclonal antibody was raised


of mouse CIDE-A (1).

APPLICATION:

This polyclonal antibody can be used for detection of CIDE-A

by Western blot at 1:500 to 1:1000 dilution. Tissue lysate of

murine heart can be used as positive control and an


activity to CIDE-B. This antibody is for research use only.

STORAGE:

Supplied as immunoaffinity chromatography purified IgG,




CIDE-A in murine heart

tissue lysate with anti-CIDE-A


RELATED PRODUCTS:

Blocking peptide, 100 µg / 500 µl, is available for


Murine heart tissue lysate, 500 µg / 200 µl, is available for

positive control (Catalog No. 1253).

REFERENCES:




2. Inohara N, Koseki T, Chen S, Benedict MA, Nunez G.

Identification of regulatory and catalytic domains in the apoptosis

nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CIDE-B (CT)

CATALOG No.: 2091

BACKGROUND:






chromosomal DNA by activated DNase. DFF45/ICAD has

been identified as inhibitor of caspase activated DNase

DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B

(for cell death-inducing DFF-like effector A and B) were

recently identified (1). CIDE contains a new type of domain

termed CIDE-N, which has high homology with the

regulatory domains of DFF45/ICAD and DFF40/CAD (1,2).

Expression of CIDE-B induces apoptosis, which is inhibited

by DFF45. CIDE-B is a DFF45-inhibitable effector that

promotes cell death and DNA fragmentation. CIDE-B is

expressed mainly in liver and at lower levels in spleen,

kidney, peripheral blood lymphocytes, and bone marrow (1).

SOURCE:

Rabbit anti-CIDE-B (CT) polyclonal antibody was raised


of murine CIDE-B (1).

APPLICATION:

This polyclonal antibody can be used for detection of CIDE-B

by Western blot at 1:500 to 1:1000 dilution. Tissue lysate of

murine liver can be used as positive control and an


activity to CIDE-A. This antibody is for research use only.

STORAGE:





CIDE-B in murine liver tissue

lysate with anti-CIDE-B (CT)

at 1:500 dilution.

RELATED PRODUCTS:



Murine liver tissue lysate, 500 µg / 200 µl, is available for


REFERENCES:




2. Inohara N, Koseki T, Chen S, Benedict MA, Nunez G.

Identification of regulatory and catalytic domains in the apoptosis

nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CX3CR1 (NT) V28, CMKBRL1

CATALOG NO.: 2093

BACKGROUND:

CX3CR1 is one of the chemokine receptors that are required

as coreceptors for HIV infection. The genes encoding

human, murine, and rat CX3CR1 were cloned and

designated V28 and CMKBRL1, CX3CR1, and RBS11,

respectively, (1-4). The encoded seven transmembrane

protein was recently identified as the receptor for a novel

transmembrane molecule, fractalkine, and renamed CX3CR1

(5). Recently, CX3CR1 was found to serve as a coreceptor

for HIV-1 and HIV-2 envelope fusion and virus infection,

which can be inhibited by fractokine (6). CX3CR1 mediates

leukocyte migration and adhesion (5). CX3CR1 is expressed

in a variety of human tissues and cell lines (2).

SOURCE:

Rabbit anti-CX3CR1 polyclonal antibody was raised against


CX3CR1 (1,2). The sequence differs from those of mouse

and rat CX3CR1 by four amino acids (3,4)

APPLICATION:


CX3CR1 by Western blot at 1:500 to 1:2000 dilution. Human

spleen tissue lysate can be used as positive control and an

approximately 50 kDa band can be detected. It is human,

mouse, and rat reactive. For research use only.

STORAGE:





CX3CR1 in human spleen

lysate with anti-CX3CR1 in the

absence (lane 1) or presence

of blocking peptide (lane 2).

REFERENCES: 1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The

orphan G-protein-coupled receptor-encoding gene V28 is closely related to

genes for chemokine receptors and is expressed in lymphoid and neural

tissues. Gene 1995;163:295-9

2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization,

and RNA expression of a human beta chemokine receptor-like gene. DNA

Cell Biol 1995;14:673-80

3. Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA

distribution, and functional expression of mCX3CR1, a mouse chemotactic

receptor for the CX3C chemokine fractalkine. Biochem Biophys Res

Commun 1998;253:728-32

4. Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled

receptor expressed in rat spinal cord and brain related to chemokine

receptors. Neurosci Lett 1994;169:85-9

5. Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki

M, Takagi S, Nomiyama H, Schall TJ, Yoshie O. Identification and molecular

characterization of fractalkine receptor CX3CR1, which mediates both

leukocyte migration and adhesion. Cell 1997;91:521-30

6. Combadiere C, Salzwedel K, Smith ED, Tiffany HL, Berger EA, Murphy

PM. Identification of CX3CR1. A chemotactic receptor for the human CX3C

chemokine fractalkine and a fusion coreceptor for HIV-1. J Biol Chem

1998;273:23799-804 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CCR8 TER1, CKR-L1, ChemR1

CATALOG NO.: 2097

BACKGROUND:

CCR8 is one of the chemokine receptors that are required as

coreceptors for HIV infection. The genes encoding human

and murine CCR8 were cloned and designated TER1, CKR-

L1, and ChemR1 (1-4). The encoded seven transmembrane

protein was identified as the receptor for human CC

chemokine I-309 and renamed CCR8. Recently, CCR8 was

found to serve as a coreceptor for diverse T-cell tropic, dual-

tropic and macrophage-tropic HIV-1 strains (5). CCR8

mediates CC chemokine I-309 induced monocyte

chemoattraction and HIV-1 envelope fusion and virus

infection, which can be prevented by the CCR8 ligand I-309.

CCR8 is expressed in spleen, thymus and T lymphoblastic

cell lines.

SOURCE:

Rabbit anti-CCR8 polyclonal antibody was raised against a


CCR8, which locate in the second extracellular loop (1-3).

APPLICATION:

This polyclonal antibody can be used for detection of CCR8

by Western blot at 1:500 to 1:1000 dilution. Human spleen

tissue lysate can be used as positive control and an

approximately 50 kDa band can be detected. For research

use only.

STORAGE:





CCR8 in human spleen lysate

with anti-CCR8 at 1:500

dilution.

REFERENCES:

1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A,

Storlazzi CT, Rocchi M, Santoni A. Molecular cloning of TER1, a

chemokine receptor-like gene expressed by lymphoid tissues. J

Immunol 1996;157:2759-63

2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G.

Molecular cloning and RNA expression of two new human

chemokine receptor-like genes. Biochem Biophys Res Commun

1996;227:846-53

3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G,

Parmentier M. Molecular cloning and chromosomal mapping of a

novel human gene, ChemR1, expressed in T lymphocytes and

polymorphonuclear cells and encoding a putative chemokine

receptor. Eur J Immunol 1996;26:3021-8

4. Goya I, Gutierrez J, Varona R, Kremer L, Zaballos A, Marquez

G. Identification of CCR8 as the specific receptor for the human

beta-chemokine I-309: cloning and molecular characterization of

murine CCR8 as the receptor for TCA-3. J Immunol

1998;160:1975-81

5. Horuk R, Hesselgesser J, Zhou Y, Faulds D, Halks-Miller M,

Harvey S, Taub D, Samson M, Parmentier M, Rucker J, Doranz

BJ, Doms RW. The CC chemokine I-309 inhibits CCR8-dependent

infection by diverse HIV-1 strains. J Biol Chem 1998;273:386-91

(WD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-GPR15 (NT) BOB

CATALOG NO.: 2101

BACKGROUND:


require coreceptors to infect target cells. Some G protein-

coupled receptors including CCR5, CXCR4, CCR3, CCR2b

and CCR8 in the chemokine receptor family, and four new

human molecules GPR15, STRL33, GPR1 and V28 were

recently identified as HIV coreceptors. The genes encoding

human and monkey GPR15/BOB (for G protein-coupled

receptor 15 and brother of Bonzo, respectively,) were

recently cloned (1,2). This novel G protein-coupled receptor

serves as corecptor for simian immunodeficiency virus (SIV),

and for strains of HIV-2 and M-tropic HIV-1 (2,3). The ligand

for GPR15 has not been identified yet.

SOURCE:

Rabbit anti-GPR15/BOB polyclonal antibody was raised


human GPR15 (1). The sequence differs from those of

African green monkey and pig-tailed macaque BOB by one

amino acid (2)

APPLICATION:


GPR15/BOB by Western blot at 1:500 to 1:2000 dilution.

Jurkat or A549 cell lysate can be used as positive control

and an approximately 50 kDa band can be detected. It is


STORAGE:




Western blot analysis of GPR15 in

human heart lysate with anti-

GPR15 (NT) in the absence (lane

1) or presence of specific peptide

(lane 2) or non-related peptide

(lane 3).

REFERENCES:

1. Heiber M, Marchese A, Nguyen T, Heng HH, George SR,

O'Dowd BF. A novel human gene encoding a G-protein-

coupled receptor (GPR15) is located on chromosome 3. Genomics

1996;32:462-5




3. Farzan M, Choe H, Martin K, Marcon L, Hofmann W, Karlsson

G, Sun Y, Barrett P, Marchand N, Sullivan N, Gerard N, Gerard C,

Sodroski J. Two orphan seven-transmembrane segment receptors

which are expressed in CD4-positive cells support simian

immunodeficiency virus infection. J Exp Med 1997;186:405-11

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DFF40/CAD (I18)

CATALOG No.: 2107

BACKGROUND:










recently identified by three groups independently and termed

CPAN, DFF40, and human CAD, respectively, (3-5).

DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6)

and forms complex with DFF40/CAD. Upon cleavage of

DFF45/ICAD by activated caspase, DFF40/CAD is released

and activated and eventually causes the degradation of DNA

in the nuclei. Activation of DFF40/CAD, which causes DNA


SOURCE:

Rabbit anti-CAD (I18) polyclonal antibody was raised against


CAD (1). The sequence differs from human DFF40 by two

amino acids (3,4)

APPLICATION:


DFF40/CAD by Western blot at 1:500 to 1:1000 dilution.

K562 or Jurkat whole cell lysate can be used as positive

control and a 40 kDa band can be detected. It is human,


STORAGE:


in 200 µl of PBS containing 0.02% sodium azide. Store at

4°C, stable for one year.


DFF40/CAD in HeLa (H),

K562 (K), Jurkat (J), and

Raji (R) whole cell lysate

with anti-DFF40/CAD (I18)

at 1:500 dilution.

REFERENCES:






1998;391:96-99














(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKα/IKK-1 (C2)

CATALOG No.: 2115

BACKGROUND:





response to extracellular stimuli including IL-1, TNFα and










expressed in a variety of human tissues.

SOURCE:




sequence by one amino acid.

APPLICATION:



from HeLa cells can be used as positive control and an 85


IKKβ or IKKγ. For research use only.

STORAGE:





IKKα in HeLa whole cell

lysate with anti-IKKα (C2)

at 1:500 dilution.

REFERENCES:






1997;90:373-83











activation. Science 1997;278:860-6 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKα (C3)

CATALOG No.: 2117

BACKGROUND:
















SOURCE:




sequence by one amino acid.

APPLICATION:



from Jurkat cells can be used as positive control and an 85


IKKβ or IKKγ. For research use only.

STORAGE:





IKKα in Jurkat whole cell

lysate with anti-IKKα (C3)

at 1:500 dilution.

REFERENCES: 1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A





1997;90:373-83











activation. Science 1997;278:860-6 (RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKβ (C3)

CATALOG No.: 2121

BACKGROUND:













with each other and both are essential for NF-κB activation.

IKKβ phosphorylates both IκB-α and IκB-β. IKKβ is

expressed in variety of human tissues.

SOURCE:

Rabbit anti-IKKβ (C3) polyclonal antibody was raised against


IKKβ (3,4), which differs from corresponding murine

sequence by one amino acid (5).

APPLICATION:

This polyclonal antibody can be used for detection of IKKβ


from Jurkat cells can be used as positive control and an 87


IKKα or IKKγ. For research use only.

STORAGE:




Western blot analysis of IKKβ

in Jurkat whole cell lysate with

anti-IKKβ (C3) at 1:500

dilution.

REFERENCES:






1997;90:373-83











activation. Science 1997;278:860-6

6. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita

H, Okumura K. Differential regulation of IkappaB kinase alpha and

beta by two upstream kinases, NF-kappaB-inducing kinase and

mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl

Acad Sci U S A 1998;95:3537-42

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IRAK2 (C2)

CATALOG No.: 2123

BACKGROUND:

The pro-inflammatory cytokine IL-1 induces cellular

response through two subunits of its receptor, IL-1 receptor I

(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1

receptor-associated kinase (IRAK) mediates activation of

NF-κB, which is a pivotal transcription factor mediating

inflammatory and immune response. A novel member in the

IRAK/Pelle family was recently identified and designated

IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of

the IL-1R complex after IL-1 binding and lead to NF-κB

activation. IRAKs also associate with Toll like receptor (TLR)

and the dominant negative mutants of IRAKs inhibit LPS-

induced NF-κB activation (2,3). Members in IRAK/Pelle

family play a central role in IL-1R and TLR mediated

inflammatory response. IRAK2 is expressed in a variety of

human tissues.

SOURCE:

Rabbit anti-IRAK2 (C2) polyclonal antibody was raised

against a peptide corresponding to amino acid 546 to 564 of

human IRAK2 (1).

APPLICATION:

This polyclonal antibody can be used for detection of IRAK2


from HeLa or K562 cells can be used as positive control and

a 65 kDa band should be detected. Anti-IRAK2 has no cross

response to IRAK. For research use only.

STORAGE:





IRAK2 in HeLa (H) and K562

(K) whole cell lysate with anti-

IRAK2 (C2) at 1:500 dilution.

REFERENCES:

1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member

IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.

Science 1997;278:1612-5

2. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E,

Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial

lipopolysaccharide activates nuclear factor-kappaB through

interleukin-1 signaling mediators in cultured human dermal

endothelial cells and mononuclear phagocytes. J Biol Chem

1999;274:7611-4

3. Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events

induced by lipopolysaccharide-activated toll-like receptor 2. J

Immunol 1999;163:639-43

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-MyD88 (IN)

CATALOG No.: 2125

BACKGROUND:

The pro-inflammatory cytokine IL-1 induced cellular

response requires IL-1 receptor complex including IL-1RI

and IL-1RAcP. Recently, MyD88 was identified as an

adapter molecule in the IL-1 signaling pathway (1). MyD88

associates with and recruits IRAK to the IL-1 receptor

complex in response to IL-1 treatment and dominant

negative form of MyD88 attenuates IL-1R-mediated NF-κB

activation. MyD88 is also employed as a regulator molecule

by IL-18 receptor and human Toll receptor (2,3), which are

members in the Toll/IL-1R family of receptors. Targeted

disruption of the MyD88 gene results in lose of cellular

responses to IL-1 and IL-18, and MyD88-deficient mice lack

responses to bacterial product LPS that employs Toll-like

receptors 2 and 4 (TLR2 and TLR4) as the signaling

receptors. MyD88 is a general adapter protein for the

Toll/IL-1R family of receptors and plays an important role in

the inflammatory response induced by cytokines IL-1 and IL-

18 and endotoxin. MyD88 gene is expressed in many

tissues.

SOURCE:

Rabbit anti-MyD88 (IN) polyclonal antibody was raised


of human MyD88 (5), which differ from those of mouse by

two amino acids.

APPLICATION:

This polyclonal antibody can be used for detection of MyD88


from Jurkat cells can be used as positive control and an

approximately 35 kDa band can be detected. It is human and


STORAGE:





MyD88 in Jurkat whole cell

lysate with anti-MyD88 (IN)

at 1:500 dilution.

REFERENCES:



Science 1997;278:1612-5

2. Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H,

Sakagami M, Nakanishi K, Akira S. Targeted disruption of the

MyD88 gene results in loss of IL-1- and IL-18-mediated function.

Immunity 1998;9:143-50

3. Medzhitov R, Preston-Hurlburt P, Kopp E, Stadlen A, Chen C,

Ghosh S, Janeway CA Jr. MyD88 is an adaptor protein in the

hToll/IL-1 receptor family signaling pathways. Mol Cell 1998;2:253-

8

4. Kawai T, Adachi O, Ogawa T, Takeda K, Akira S.

Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity

1999;11:115-22

5. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE.

The cloning and characterization of human MyD88: amember of an

IL-1 receptor related family. FEBS Lett 1997;402:81-4

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-MyD88 (CT)

CATALOG No.: 2127

BACKGROUND:


response requires IL-1 receptor complex including IL-1RI

and IL-1RAcP. Recently, MyD88 was identified as an

adapter molecule in the IL-1 signaling pathway (1). MyD88

associates with and recruits IRAK to the IL-1 receptor

complex in response to IL-1 treatment and dominant

negative form of MyD88 attenuates IL-1R-mediated NF-κB

activation. MyD88 is also employed as a regulator molecule

by IL-18 receptor and human Toll receptor (2,3), which are

members in the Toll/IL-1R family of receptors. Targeted

disruption of the MyD88 gene results in lose of cellular

responses to IL-1 and IL-18, and MyD88-deficient mice lack

responses to bacterial product LPS that employs Toll-like

receptors 2 and 4 (TLR2 and TLR4) as the signaling

receptors. MyD88 is a general adapter protein for the

Toll/IL-1R family of receptors and plays an important role in

the inflammatory response induced by cytokines IL-1 and IL-

18 and endotoxin. MyD88 gene is expressed in many

tissues.

SOURCE:

Rabbit anti-MyD88 (CT) polyclonal antibody was raised


of human MyD88 (5), which are identical to those of mouse.

APPLICATION:

This polyclonal antibody can be used for detection of MyD88



approximately 35 kDa band can be detected. It is human and


STORAGE:





MyD88 in Jurkat whole cell

lysate with anti-MyD88 (CT)

at 1:500 dilution.

REFERENCES:



Science 1997;278:1612-5

2. Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H,

Sakagami M, Nakanishi K, Akira S. Targeted disruption of the

MyD88 gene results in loss of IL-1- and IL-18-mediated function.

Immunity 1998;9:143-50

3. Medzhitov R, Preston-Hurlburt P, Kopp E, Stadlen A, Chen C,

Ghosh S, Janeway CA Jr. MyD88 is an adaptor protein in the

hToll/IL-1 receptor family signaling pathways. Mol Cell 1998;2:253-

8

4. Kawai T, Adachi O, Ogawa T, Takeda K, Akira S.

Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity

1999;11:115-22

5. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE.

The cloning and characterization of human MyD88: a member of

an IL-1 receptor related family. FEBS Lett 1997;402:81-4

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-1RAcP (C2)

CATALOG No.: 2129

BACKGROUND:


response requires two subunits of its receptor, IL-1 receptor I

(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).

IL-1RAcP forms a complex with IL-1RI in response to IL-1

treatment. The IL-1 receptor-associated kinase (IRAK),

which mediates activation of NF-κB inducing kinae (NIK) and

of NF-κB, recruits to the IL-1R complex through IL-1RAcP

(2). IL-1 activation of stress-activated protein kinase and of

acid sphingomyelinase also requires IL-1RAcP (3,4). Like

IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated

cellular response. IL-1RAcP is expressed in many tissues.

SOURCE:

Rabbit anti-IL-1RAcP (C2) polyclonal antibody was raised


of human IL-1RAcP, which is identical to those of mouse and

rat origins (1).

APPLICATION:

This polyclonal antibody can be used for detection of IL-

1RAcP by Western blot at 1:1000 to 1:2000 dilution. Whole

cell lysate from HeLa cells can be used as positive control


human, mouse, and rat reactive, and has no cross activity to

other members in the IL-1 receptor family. For research use

only.

STORAGE:




Western blot analysis of IL-

1RAcP in HeLa whole cell

lysate with anti-IL-1RAcP (C2)

at 1:1000 dilution.

REFERENCES:

1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju

G. Molecular cloning and characterization of a second subunit of

the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-

65

2. Huang J, Gao X, Li S, Cao Z. Recruitment of IRAK to the

interleukin 1 receptor complex requires interleukin 1 receptor

accessory protein. Proc Natl Acad Sci U S A 1997;94:12829-32

3. Wesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU.

The interleukin-1 receptor accessory protein (IL-1RAcP) is

essential for IL-1-induced activation of interleukin-1 receptor-

associated kinase (IRAK) and stress-activated protein kinases

(SAP kinases). J Biol Chem 1997;272:7727-31

4. Hofmeister R, Wiegmann K, Korherr C, Bernardo K, Kronke M,

Falk W. Activation of acid sphingomyelinase by interleukin-1 (IL-1)

requires the IL-1 receptor accessory protein. J Biol Chem

1997;272:27730-6

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-1RAcP (CT)

CATALOG No.: 2131

BACKGROUND:


response requires two subunits of its receptor, IL-1 receptor I

(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).

IL-1RAcP forms a complex with IL-1RI in response to IL-1

treatment. The IL-1 receptor-associated kinase (IRAK),

which mediates activation of NF-κB inducing kinae (NIK) and

of NF-κB, recruits to the IL-1R complex through IL-1RAcP

(2). IL-1 activation of stress-activated protein kinase and of

acid sphingomyelinase also requires IL-1RAcP (3,4). Like

IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated

cellular response. IL-1RAcP is expressed in many tissues.

SOURCE:

Rabbit anti-IL-1RAcP (CT) polyclonal antibody was raised


of human IL-1RAcP, which differs from the sequence of

mouse origin by three amino acids (1).

APPLICATION:

This polyclonal antibody can be used for detection of IL-

1RAcP by Western blot at 1:500 to 1:1000 dilution. Whole

lysate from HeLa cells can be used as positive control and

an approximately 66 kDa band can be detected. It has no

cross activity to other members in the IL-1 receptor family.

For research use only.

STORAGE:





1RAcP in HeLa whole cell

lysate with anti-IL-1RAcP


REFERENCES:

1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju

G. Molecular cloning and characterization of a second subunit of

the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-

65

2. Huang J, Gao X, Li S, Cao Z. Recruitment of IRAK to the

interleukin 1 receptor complex requires interleukin 1 receptor

accessory protein. Proc Natl Acad Sci U S A 1997;94:12829-32

3. Wesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU.

The interleukin-1 receptor accessory protein (IL-1RAcP) is

essential for IL-1-induced activation of interleukin-1 receptor-

associated kinase (IRAK) and stress-activated protein kinases

(SAP kinases). J Biol Chem 1997;272:7727-31

4. Hofmeister R, Wiegmann K, Korherr C, Bernardo K, Kronke M,

Falk W. Activation of acid sphingomyelinase by interleukin-1 (IL-1)

requires the IL-1 receptor accessory protein. J Biol Chem

1997;272:27730-6

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-APP (Aβ-NT)

CATALOG NO.: 2133

BACKGROUND:

Accumulation of the amyloid-β peptide (Aβ) in the cerebral

cortex is a critical event in the pathogenesis of Alzheimer’s

disease. The β−amyloid protein precursor (APP) is cleaved

by β−secretase, producing a soluble derivative of the protein

and a membrane anchored 99-amino acid carboxy-terminal

fragment (C99). The C99 fragment serves as substrate for

γ−secretase to generate the 4 kDa amyloid-β peptide (Aβ),

which is deposited in the brains of all suffers of Alzheimer’s

disease.

SOURCE:

Rabbit anti-APP (Aβ-NT) polyclonal antibody was raised


of human amyloid A4 protein precursor (APP) (1) or 1 to 10

of the 4K Aβ peptide generated by β− and γ−secretases (2).

The peptide sequences are identical to those of rabbit, pig,

bovine, guinea pig, and chicken.

APPLICATION:

This polyclonal antibody can be used for detection of APP

and Aβ peptide by Western blot at 1:500 to 1:1000 dilution.

Murine brain lysate can be used as positive control. It is

human, mouse, and rat reactive. This antibody is for

research use only.

STORAGE:




RELATED PRODUCTS:



Murine brain tissue lysate, 500 µg / 200 µl, is available for


Western blot analysis of APP

in human (A), mouse (B), and

rat (C) brain tissue lysates

with anti-APP (Aβ-NT) at

1:500 dilution.

REFERENCES: 1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D,

Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new

A4 amyloid mRNA contains a domain homologous to serine

proteinase inhibitors. Nature 1988;331:525-7.

2. Selkoe D.J. Cell biology of the amyloid beta-protein precursor

and the mechanism of Alzheimer's disease. Annu Rev Cell Biol

1994;10:373-403

(RD1000)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-APP (CT)

CATALOG NO.: 2136

BACKGROUND:

Accumulation of the amyloid-β peptide (Aβ) in the cerebral


disease. The β−amyloid protein precursor (APP) is cleaved

by β−secretase, producing a soluble derivative of the protein

and a membrane anchored 99-amino acid carboxy-terminal


γ−secretase to generate the 4 kDa amyloid-β peptide (Aβ),

which is deposited in the brains of all suffers of Alzheimer’s

disease.

SOURCE:

Rabbit anti-APP (CT) polyclonal antibody was raised against


amyloid A4 protein precursor (APP) (1) or 85 to 99 of the

C99 fragment generated by β− secretase cleavage (2). The

peptide sequences are identical to those of monkey, mouse,

rat, chicken, and a variety of other species.

APPLICATION:

This polyclonal antibody can be used for detection of APP

and the C99 fragment by Western blot at 1:500 to 1:1000

dilution. Murine brain lysate can be used as positive control.

It is human, mouse, and rat reactive. This antibody is for

research use only.

STORAGE:





APP in mouse (M) and rat

(R) brain tissue lysates with

anti-APP (CT) at 1:500

dilution.

RELATED PRODUCTS:



Murine brain tissue lysate, 500 µg / 200 µl, is available for


REFERENCES: 1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D,

Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new

A4 amyloid mRNA contains a domain homologous to serine

proteinase inhibitors. Nature 1988;331:525-7

2. Selkoe D.J. Cell biology of the amyloid beta-protein precursor

and the mechanism of Alzheimer's disease. Annu Rev Cell Biol

1994;10:373-403

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DcR3 (NT) TR6

CATALOG No.: 2140

BACKGROUND:



containing receptors. Several novel members in the TNFR

family including DR3, DR4, DR5, and DR6 were recently

discovered and function as cell death receptors. Two decoy

receptors, DcR1 and DcR2, were recently identified to

compete with DR4 and DR5 for their ligand TRAIL binding. A

novel decoy receptor was more recently discovered and

designated DcR3 and TR6, respectively, (1,2). Unlike DcR1

and DcR2, DcR3 is a soluble rather than a membrane

associated molecule. DcR3 binds to FasL and LIGHT and

inhibits FasL and LIGHT induced apoptosis (1,2). DcR3

transcript is expressed in a number of lung and colon

carcinomas and in some normal tissues.

SOURCE:

Rabbit anti-DcR3/TR6 (NT) polyclonal antibody was raised


human DcR3 precursor (1,2).

APPLICATION:



Tissue lysate of human heart can be used as positive control



STORAGE:

It is supplied as ion exchange chromatography purified IgG,


Store at -20°C. Stable for one year at 2-8°C.

Western blot analysis of DcR3

in human heart (H), brain (B),

and kidney (K) tissue lysates

with anti-DcR3 (NT) at 1:500

dilution.

REFERENCES:

1. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd

P, Huang A, Donahue CJ, Sherwood SW, Baldwin DT, Godowski

PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD,

Botstein D, Ashkenazi A. Genomic amplification of a decoy

receptor for Fas ligand in lung and colon cancer. Nature

1998;396:699-703

2. Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly

identified member of tumor necrosis factor receptor superfamily

(TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem

1999;274:13733-6

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-SODD (NT)

CATALOG No.: 2143

BACKGROUND:


Fas ligand of the TNF family through their death domain

containing receptors, TNF-R1 and Fas. Several novel death

receptors including DR3, DR4, DR5, and DR6 were recently

identified. Cell death signal is transduced by death domain

containing adapter molecules through the interaction with

death domain of these death receptors. A novel TNF-R1

interacting protein was recently identified and designated

SODD for silencer of death domains (1). SODD associates

with the death domain of TNF-R1 and prevents constitutive

activation of TNF-R1 signaling. TNF treatment releases

SODD and permits adapter molecules such as TRADD

recruiting to the active TNF-R1 complex, which activates

TNF signaling pathways. SODD also interacts with DR3.

SODD is ubiquitously expressed in human tissues and cell

lines.

SOURCE:

Rabbit anti-SODD (NT) polyclonal antibody was raised


human SODD (1).

APPLICATION:

This polyclonal antibody can be used for detection of SODD

by Western blot at 1:500 to 1:2000 dilution. HeLa and THP-1

whole cell lysate can be used as positive control and an



STORAGE:



year.

Western blot analysis of SODD

in HeLa (1,3) and THP-1 (2,4)

whole cell lysates in the

absence (1,2) or presence (3,4)

of blocking peptide (Catalog no.

2143P) with anti-SODD (NT) at

1:500 dilution.

REFERENCES:

1. Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of

constitutive TNF receptor 1 signaling by silencer of death domains.

Science 1999;283:543-6

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DRAK1 (NT)

CATALOG No.: 2147

BACKGROUND:




are mediators of apoptosis. Two novel serine/threonine

kinases that induce apoptosis were recently identified and

designated DRAK1 and DRAK2 for DAP kinase-related

apoptosis-inducing protein kinases (1). DRAKs contain an N-

terminal kinase domain and a C-terminal regulation domain.

Overexpression of DRAK1 induces apoptosis. DRAKs have

high sequence homology to DAP and ZIP kinases, and they

represent a novel family of serine/threonine kinases, which

mediates apoptosis through their catalytic activities. DRAK1

is located in nucleus and the messenger RNA was

ubiquitously expressed in human tissues (1).

SOURCE:

Rabbit anti-DRAK1 (NT) polyclonal antibody was raised


human DRAK1 (1).

APPLICATION:

This polyclonal antibody can be used for detection of DRAK1

by Western blot at 1:500 to 1:1000 dilution. A431 or MOLT4



mouse, and rat reactive, and has no cross responses to

DRAK2, DAP or ZIP kinases. For research use only.

STORAGE:





DRAK1 in MOLT4 (A),

A431 (B), and 3T3 (C)

whole cell lysates with

anti-DRAK1 (2147) at

1:500 dilution.

REFERENCES:

1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine

kinases related to death-associated protein kinase that trigger


(WD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DRAK2 (CT)

CATALOG No.: 2149

BACKGROUND:




are mediators of apoptosis. Two novel serine/threonine

kinases that induce apoptosis were recently identified and

designated DRAK1 and DRAK2 (for DAP kinase-related

apoptosis-inducing protein kinases) (1). DRAKs contain an

N-terminal kinase domain and a C-terminal regulation

domain. Overexpression of DRAK2 induces apoptosis.

DRAKs have high sequence homology to DAP and ZIP

kinases, and they represent a novel family of

serine/threonine kinases, which mediates apoptosis through

their catalytic activities. DRAK2 is located in nucleus and the

messenger RNA was ubiquitously expressed in human

tissues (1).

SOURCE:

Rabbit anti-DRAK2 (CT) polyclonal antibody was raised


of human DRAK2 (1).

APPLICATION:

This polyclonal antibody can be used for detection of DRAK2




responses to DAP or ZIP kinases. The approximately 70 kDa

band is probably non-related to DRAK2 although it is peptide

blockable. For research use only.

STORAGE:





DRAK2 in Jurkat (1,3) and

Raji (2,4) cell lysate in the

absence (1,2) or presence

(3,4) of blocking peptide with

anti-DRAK2 (CT) at 1:500

dilution.

REFERENCES:

1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine

kinases related to death-associated protein kinase that trigger


(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DFF40 (NT) CPAN, hCAD

CATALOG No.: 2153

BACKGROUND:








designated CAD for caspase activated deoxyribonuclease










SOURCE:

Rabbit anti-DFF40 (NT) polyclonal antibody was raised


human DFF40 (4, 5).

APPLICATION:


by Western blot at 1:500 to 1:2000 dilution. K562 or Jurkat


kDa band can be detected. It is human and mouse reactive.


STORAGE:





DFF40 in Jurkat (J) and

K562 (K) whole cell lysate

with anti-DFF40 (NT) at

1:500 dilution.

REFERENCES: 1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,





1998;391:96-99









Nagata S. Molecular cloning and characterization of human

caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-

8




(WD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DFF40 (IN) CPAN, hCAD

CATALOG No.: 2155

BACKGROUND:








designated CAD for caspase activated deoxyribonuclease










SOURCE:

Rabbit anti-DFF40 (IN) polyclonal antibody was raised


of human DFF40 (4, 5).

APPLICATION:


/CAD by Western blot at 1:500 dilution. K562 or Jurkat whole

cell lysate can be used as positive control and a 40 kDa

band can be detected. It is human, mouse, and rat reactive,


STORAGE:




REFERENCES: 1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,





1998;391:96-99














(WD0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DR6 (NT)

CATALOG No.: 2157

BACKGROUND:


Fas ligand of the TNF family through their death domain

containing receptors, TNF-R1 and Fas. Several novel death

receptors including DR3, DR4, and DR5 were recently

identified. A new death domain containing receptor in the

TNFR family was cloned recently and termed DR6 for death

receptor-6 (1). Like TNF-R1, DR6 interacts with death

domain containing adapter molecule TRADD.

Overexpression of DR6 induces apoptosis and activates NF-

κB and JNK. DR6 is widely expressed in human tissues and

cell lines (1). The ligand for DR6 has not been identified.

SOURCE:



DR6 precursor (1).

APPLICATION:


Western blot at 1:500 to 1:2000 dilution. HeLa and K562


approximately 68 kDa band can be detected. For research

use only.

STORAGE:



year.


in K562 (1,3) and Raji (2,4)

whole cell lysate in the

absence (1,2) or presence

(3,4) of blocking peptide

(Catalog no. 2157P) with anti-

DR6 (NT) at 1:500 dilution.

REFERENCES:

1. Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C,

Aggarwal BB, Ni J, Dixit VM. Identification and functional

characterization of DR6, a novel death domain-containing TNF

receptor. FEBS Lett 1998;431:351-6

(RD1299)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bcl10 (NT) CIPER, mE10, CARMEN, CLAP

CATALOG No.: 2161

BACKGROUND:

Apoptosis is related to many diseases including cancer. Cell

death signals are transduced by death domain (DD) and


and a caspase family of proteases. CARD containing cell

death regulators include ARC, RAIDD, Apaf-1, caspase-9,

and caspase-2. A novel CARD containing protein was

recently identified by several groups and designated Bcl10,

CIPER, mE10, CARMEN, CLAP (1-6). Bcl10 is a cellular

homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-κB

activation (1,2,4,6). Bcl10 interacts with caspase-9 and

enhances pro-caspase-9 processing (3) and induces

apoptosis through caspase-9 activation (3.6). Bcl10 exhibits

a variety of mutations in MALT lymphomas and in B and T

cell lineage lymphomas indicating that it may be commonly

involved in the pathogenesis of human malignancy (1,5).

Bcl10 is expressed in many human and murine tissues and

cell lines (2-6).

SOURCE:

Rabbit anti-BCL10 (NT) polyclonal antibody was raised


human origin (1-6).

APPLICATION:

This polyclonal antibody can be used for detection of BCL10

by Western blot at 1:500 dilution. Raji whole cell lysate can

be used as positive control and an approximately 31 kDa

band can be detected. For research use only.

STORAGE:




RELATED PRODUCT:

Blocking peptide, 50 µg/250 µl, is available for competition studies (Catalog No. 2161P).

Raji cell lysate, 200 µg/100 µl, is available for positive


Western blot analysis of Bcl-10

in Raji whole cell lysate in the

absence (A) or presence (B) of

peptide (2161P) with anti-Bcl-10

(NT) at 1:500 dilution.

REFERENCES: 1. Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in

t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple

tumor types. Cell 1999;96(1):35-45

2. Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF kappaB-

activating protein containing a caspase recruitment domain with

homology to Herpesvirus-2 protein E10. J Biol Chem

1999;274(15):9955-61

3. Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel

caspase recruitment domain-containing proapoptotic molecule. J

Biol Chem 1999;274(15):10287-92

4. Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2

E10 gene product, but not its cellular homologue, activates NF-

kappaB transcription factor and c-Jun N-terminal kinase. J Biol

Chem 1999;274(15):9962-8

5. Zhang Q, Siebert R, Yan M, Hinzmann B, et al. Inactivating

mutations and overexpression of BCL10, a caspase recruitment

domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32).

Nat Genet 1999;22(1):63-8

6. Srinivasula SM, Ahmad M, Lin JH, et al. CLAP, a novel caspase

recruitment domain-containing protein in the tumor necrosis factor

receptor pathway, regulates NF-kappaB activation and apoptosis. J

Biol Chem 1999;274(25):17946-54

(WD0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DcR1 (ED) TRAIL-R3, TRID, LIT

CATALOG No.: 2179

BACKGROUND:






receptors for TRAIL (1-3). Two decoy receptors for TRAIL

have been identified and designated DcR1/TRID/TRAIL-

R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1

has extracellular TRAIL-binding domain but lacks

intracellular signaling domain. It is a glycophospholipid-

anchored cell surface protein. DcR1 transcripts were

expressed in many normal human tissues but not in most

cancer cell lines (2,3). Overexpression of DcR1 did not

induce apoptosis, but attenuated TRAIL-induced apoptosis

(2,3).

SOURCE:

Rabbit anti-DcR1 (ED) polyclonal antibody was raised


at extracellular domain (ED) of human DcR1 precursor (2-3).

APPLICATION:


by Western blot at 1:500 to 1:1000 dilution. HeLa cell lysate

can be used as positive control and an approximate 65 kDa

band can be detected (6). It is human, mouse, and rat


STORAGE:

It is supplied as chromatography purified IgG, 100 µg in 200



Western blot analysis of DcR1


anti-DcR1 (ED) at 1:500 dilution.

REFERENCES: 1. Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic

ligand TRAIL. Science; 1997;276:111-113

2. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death

domain-containing receptor for TRAIL. Science 1997;277:815-8

3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced


1997;277:818-21

4. Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and

characterization of TRAIL-R3, a novel member of the emerging TRAIL

receptor family. J Exp Med 1997;186(7):1165-70

5. MacFarlane M, Ahmad M, Srinivasula SM, et al. Identification and

molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J

Biol Chem 1997;272(41):25417-20

6. Schneider P, Bodmer JL, Thome M, Hofmann K, Holler N, Tschopp J.

Characterization of two receptors for TRAIL. FEBS Lett 1997;416(3):329-34

7. Mongkolsapaya J, Cowper AE, Xu XN, et al. Lymphocyte inhibitor of

TRAIL (TNF-related apoptosis -inducing ligand): a new receptor protecting

lymphocytes from the death ligand TRAIL. J Immunol 1998;160(1):3-6

8. Marsters SA, Sheridan JP, Pitti RM, et al. A novel receptor for

Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6

9. Degli-Esposti MA, Dougall WC, Smolak PJ, et al. The novel receptor

TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated

apoptosis, yet retains an incomplete death domain. Immunity 1997;7:813-20

10. Pan G, Ni J, Yu G, et al. TRUNDD, a new member of the TRAIL

receptor family that antagonizes TRAIL signalling. FEBS Lett 1998;424:41-5

(RD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-RICK (CT) RIP2, CARDIAK

CATALOG No.: 2183

BACKGROUND:

Apoptosis is mediated by death domain (DD) and/or caspase


caspase family of proteases. DD-containing serine/threonine

kinase RIP regulates Fas-induced apoptosis. A novel CARD-

containing serine/threonine kinase was recently identified

and designated RICK/RIP2/CARDIAK for RIP-like interacting

CLARP kinase, receptor interacting protein-2, and CARD-

containing ICE associated kinase, respectively, (1-3). RICK

contains an N-terminal kinase catalytic domain and a C-

terminal CARD domain. Overexpression of RICK induced

apoptosis and activation of NF-κB and JNK. RICK interacts

with members of the TRAF family, CLARP and caspase-1.

Thus, RICK represents a novel kinase that regulates TNF

and Fas induced-apoptosis and that is involved in the

generation of proinflammatory cytokine IL-1β. The

messenger RNA of RICK is expressed in multiple human

tissues (1).

SOURCE:

Rabbit anti-RICK (CT) polyclonal antibody was raised



APPLICATION:

This polyclonal antibody can be used for detection of RICK

by Western blot at 1:500 dilution. K562 or 3T3 whole cell


60 kDa band can be detected. It is human, mouse, and rat


STORAGE:




REFERENCES:

1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a

novel protein kinase containing a caspase recruitment domain,

interacts with CLARP and regulates CD95-mediated apoptosis. J

Biol Chem 1998;273:12296-300

2. McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-kappaB-

activating and cell death-inducing kinase. J Biol Chem

1998;273:16968-75

3. Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL,

Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like

kinase that associates with caspase-1. Curr Biol 1998;8:885-8

(WD0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ARC (NT)

CATALOG No.: 2185

BACKGROUND:

Apoptosis is regulated by death domain (DD) and/or


and a caspase family of proteases. CARD containing cell

death regulators include RAIDD, RICK BCL10, Apaf-1,

caspase-9, and caspase-2. A novel CARD domain

containing protein was recently identified and designated

ARC for apoptosis repressor with CARD (1). ARC interacts

with caspase-2 and –8 and inhibits enzymatic activity of

caspase-8. ARC suppresses apoptosis induced by cell death

adapters FADD and TRADD and by cell death receptors

Fas, TNFR-1, and DR3. The messenger RNA of ARC is

primarily expressed in skeletal muscle and cardiac tissue (1).

SOURCE:

Rabbit anti-ARC (NT) polyclonal antibody was raised against


origin (1). These sequences are identical to those of human

nuclear protein Nop30 (2) and differ from those of the rat

homolog of ARC by one amino acid (3)

APPLICATION:

This polyclonal antibody can be used for detection of ARC


HeLa cells can be used as positive control and an



STORAGE:




RELATED PRODUCT:



HeLa cell lysate, 200 µg/100 µl, is available for positive


Western blot analysis of ARC in

HeLa whole cell lysates with anti-

ARC (NT) at 1:500 dilution.

REFERENCES:

1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of

apoptosis expressed in skeletal muscle and heart that interacts

selectively with caspases. Proc Natl Acad Sci USA 1998;95:5156-

60

2. Stoss O, Schwaiger FW, Cooper TA, Stamm S. Alternative

splicing determines the intracellular localization of the novel

nuclear protein Nop30 and its interaction with the splicing factor

SRp30c. J Biol Chem 1999;274(16):10951-62

3. Geertman R, McMahon A, Sabban EL. Cloning and

characterization of cDNAs for novel proteins with glutamic acid-

proline dipeptide tandem repeats. Biochim Biophys Acta

1996;1306(2-3):147-52

(WD 0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CX3CR1 (EL) V28, CMKBRL1

CATALOG No.: 2201

BACKGROUND:

CX3CR1 is one of the chemokine receptors that are required

as coreceptors for HIV infection. The genes encoding

human, mouse, and rat CX3CR1 were cloned and

designated V28 and CMKBRL1, CX3CR1, and RBS11,

respectively, (1-4). The encoded seven transmembrane

protein was recently identified as the receptor for a novel

transmembrane molecule, fractalkine, and renamed CX3CR1

(5). Recently, CX3CR1 was found to serve as a coreceptor

for HIV-1 and HIV-2 envelope fusion and virus infection,

which can be inhibited by fractokine (6). CX3CR1 mediates

leukocyte migration and adhesion (5). CX3CR1 is expressed

in a variety of human tissues and cell lines (2).

SOURCE:

Rabbit anti-CX3CR1 polyclonal antibody was raised against


CX3CR1 (1,2). The sequence is identical to that of rat

CX3CR1 and differs from that of mouse CX3CR1 by one

amino acid (3,4).

APPLICATION:


CX3CR1 by Western blot at 1:500 dilution. THP-1 cell lysate

can be used as positive control and an approximately 50


STORAGE:

It is supplied as 100 µg of immunoaffinity chromatography

purified IgG in 200 µl of PBS containing 0.02% sodium


RELATED PRODUCT:



THP-1 cell lysate, 200 µg/100 µl, is available for positive



CX3CR1 in THP-1 cell lysate in

the absence (lane A) or

presence (lane B) of blocking

peptide with anti-CX3CR1 (EL)

at 1:500 dilution.

REFERENCES: 1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The

orphan G-protein-coupled receptor-encoding gene V28 is closely related to

genes for chemokine receptors and is expressed in lymphoid and neural

tissues. Gene 1995;163:295-9

2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization,

and RNA expression of a human beta chemokine receptor-like gene. DNA

Cell Biol 1995;14:673-80

3. Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA

distribution, and functional expression of mCX3CR1, a mouse chemotactic

receptor for the CX3C chemokine fractalkine. Biochem Biophys Res

Commun 1998;253:728-32

4. Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled

receptor expressed in rat spinal cord and brain related to chemokine

receptors. Neurosci Lett 1994;169:85-9

5. Imai T, Hieshima K, Haskell C, et al. Identification and molecular

characterization of fractalkine receptor CX3CR1, which mediates both

leukocyte migration and adhesion. Cell 1997;91:521-30

6. Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A

chemotactic receptor for the human CX3C chemokine fractalkine and a

fusion coreceptor for HIV-1. J Biol Chem 1998;273:23799-804 (RD0400)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bonzo (NT2) STRL33, TYMSTR

CATALOG NO.: 2209

BACKGROUND:

Human immunodeficiency virus (HIV) and simian

immunodeficiency virus (SIV) require coreceptors, in addition

to CD4, to infect target cells. Some G protein-coupled

receptors including CCR5, CXCR4, CCR3, and CCR2b in

the chemokine receptor family have been identified as HIV

coreceptors. An orphan G protein-coupled receptor was

recently cloned and designated Bonzo, STRL33 and

TYMSTR, and identified as HIV and SIV coreceptor (1-4).

Bonzo/STRL33 serves as coreceptor for SIV, HIV-2 and

HIV-1. The messenger RNA of Bonzo/STRL33 is expressed

in lymphoid tissues and activated peripheral blood

lymphocytes.

SOURCE:

Rabbit anti-Bonzo polyclonal antibody was raised against a


Bonzo/STRL33 (1,2). The sequence of this peptide differs

from those of African green monkey and pig-tailed macaque

by one or two amino acids, respectively, (1).

APPLICATION:

This polyclonal antibody can be used for detection of Bonzo

by Western blot at 1:500 dilution. Human spleen tissue

lysate can be used as positive control. For research use

only.

STORAGE:

It is supplied as 100 µg of immunoaffinity purified IgG in 200



RELATED PRODUCT:



Human spleen tissue lysate, 200 µg/100 µl, is available for



Bonzo in human spleen tissue

lysate in the absence (lane A)

or presence (lane B) of

peptide with anti-Bonzo (NT2)

at 1:500 dilution.

REFERENCES:




2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber

JM. STRL33, A novel chemokine receptor-like protein, functions as

a fusion cofactor for both macrophage-tropic and T cell line-tropic

HIV-1. J Exp Med 1997;185:2015-23

3. Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new

SIV co-receptor, STRL33. Nature 1997;388:238

4. Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher

P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M,

Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine

receptor selectively expressed in activated T cells, exhibits HIV -1

coreceptor function. Curr Biol 1997;7:652-60 (RD0400)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IRAK2 (CT)

CATALOG No.: 2213

BACKGROUND:

The pro-inflammatory cytokine IL-1 induces cellular response

through two subunits of its receptor, IL-1 receptor I (IL-1RI)

and IL-1 receptor accessory protein (IL-1RAcP). IL-1

receptor-associated kinase (IRAK) mediates activation of

NF-κB (1), which is a pivotal transcription factor mediating

inflammatory and immune response. A novel member in the

IRAK/Pelle family was recently identified and designated

IRAK2 (2). Both IRAK and IRAK2 recruit to the subunits of

the IL-1R complex after IL-1 binding and lead to NF-κB

activation. IRAKs also associate with Toll-like receptor (TLR)

and the dominant negative mutants of IRAKs inhibit LPS-

induced NF-κB activation (3,4). Members in IRAK/Pelle

family play a central role in IL-1R and TLR mediated

inflammatory responses to cytokine IL-1 and LPS. IRAK2 is


SOURCE:

Rabbit anti-IRAK2 (CT) polyclonal antibody was raised


of human IRAK2 (2).

APPLICATION:

This polyclonal antibody can be used for detection of IRAK2

by Western blot at 1:500 to 1:1000 dilution. K562 whole cell


can be detected. Anti-IRAK2 has no cross response to IRAK.


STORAGE:

It is supplied as chromatography purified IgG, 100 µg in

200 µl of PBS containing 0.02% sodium azide. Store at



IRAK2 in K562 whole cell

lysate with anti-IRAK2 (CT) at

1:500 dilution.

REFERENCES: 1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the




Science 1997;278:1612-5

3. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E,

Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial

lipopolysaccharide activates nuclear factor-kappaB through

interleukin-1 signaling mediators in cultured human dermal

endothelial cells and mononuclear phagocytes. J Biol Chem

1999;274:7611-4

4. Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events

induced by lipopolysaccharide-activated toll-like receptor 2. J

Immunol 1999;163:639-43

(RD0300)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Acinus (CP)

CATALOG No.: 2215

BACKGROUND:

Chromatin condensation and nuclear fragmentation (CCNF)

is the hallmark of apoptosis. CCNF is triggered by the

activation of members of caspase family, caspase activated

DNase (CAD/DFF40), and several novel proteins including

AIF and CIDE (1). A new inducer of chromatin condensation

was recently identified and designated Acinus (for apoptotic

chromatin condensation inducer in the nucleus). Acinus is

cleaved by caspase-3 and an additional unknown protease

generating a small active peptide p17, which causes

chromatin condensation in vitro when it is added to purified

nuclei. Acinus also induces apoptotic chromatin

condensation in cells. Acinus is ubiquitously expressed.

Three different spliced forms of Acinus have been identified

in human and mouse and designated AcinusL, AcinusS and

AcinusS’ (2).

SOURCE:

Rabbit anti-Acinus (CP) polyclonal antibody was raised

against a peptide (TRTALHGVKWPQSNPK) corresponding

to amino acids 1065 to 1080 of human AcinusL, 338 to 353

of human AcinusS’, or 307 to 322 of human AcinusS, which

are identical to those of mouse Acinus (2). The selected

antigenic sequence is located near the C-terminus of the

cleaved active peptide p17.

APPLICATION:

This polyclonal antibody can be used for detection of Acinus

by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be



STORAGE:




Western blot analysis of Acinus in

K562 whole cell lysate with anti-

Acinus (CP) at 1 µg/ml.

RELATED PRODUCTS:



K562 cell lysate, 200 µg at 2 mg/ml, is available for positive


REFERENCES:

1. Zamzami N, Kroemer G. Condensed matter in cell death.

Nature 1999 ;401:127-8.

2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto

Y. Acinus is a caspase-3-activated protein required for apoptotic

chromatin condensation. Nature 1999 401:168-73. (WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Acinus (CT)

CATALOG No.: 2217

BACKGROUND:















AcinusS’ (2).

SOURCE:

Rabbit anti-Acinus (CT) polyclonal antibody was raised

against a peptide (SRSTPVRDRGGRR) corresponding to

amino acids 1329 to 1341 of human AcinusL, 602 to 614 of

human AcinusS’, or 571 to 583 of human AcinusS, which are

identical to those of mouse Acinus (2).

APPLICATION:


by Western blot at 0.25 to 0.5 µg/ml. K562 cell lysate can be



STORAGE:





Acinus in K562 whole cell

lysate with anti-Acinus (CT) at

0.5 µg/ml.

RELATED PRODUCTS:





REFERENCES:


Nature 1999 ;401:127-8.



chromatin condensation. Nature 1999 401:168-73.

(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Acinus (IN)

CATALOG No.: 2219

BACKGROUND:















AcinusS’ (2).

SOURCE:

Rabbit anti-Acinus (IN) polyclonal antibody was raised

against a peptide (DTSENRPENDVPEPP) corresponding to

amino acids 775 to 789 of human AcinusL, 48 to 62 of

human AcinusS’, or 17 to 31 of human AcinusS, which differ

from those of mouse Acinus by one amino acid (2).

APPLICATION:





STORAGE:




Western blot analysis of Acinus

in K562 whole cell lysate with

anti-Acinus (IN) at 1 µg/ml.

RELATED PRODUCTS:





REFERENCES:


Nature 1999 ;401:127-8.




(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-BAFF (CT) BLyS, TALL-1, THANK

CATALOG No.: 2221

BACKGROUND:

Members in the TNF superfamily regulate immune

responses and induce apoptosis. A novel member in the

TNF family was recently identified by several groups and

designated BAFF (for B cell Activating Factor belonging to

the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-

1 (for TNF- and ApoL-related Leukocyte-expressed Ligand),

and THANK (for TNF Homologue that Activate Apoptosis,

NF-κB and c-jun N-terminal Kinase) (1-4). BAFF/BLyS was

characterized as a B cell stimulator since it induced B cell

proliferation and immunoglobulin secretion (1,2). Two

receptors for BAFF were recently identified and designated

TACI and BCMA (5). BAFF and its receptors are involved in

the development of systemic lupus erythaematosus and

other B cell associated autoimmune diseases (5,6). Like

TNFα and TRAIL, THANK was shown to activate NF-κB and

c-jun N-terminal kinase (JNK) and to induce apoptosis (4).

SOURCE:

Rabbit anti-BAFF (CT) polyclonal antibody was raised


of human BAFF (1-4).

APPLICATION:

This polyclonal antibody can be used for detection of BAFF

by Western blot at 0.25 to 1 µg/ml. It is human, mouse and

rat reactive. For research use only.

STORAGE:



Store at 4°C, stable for one year. Azide free antibody is

available.

RELATED PRODUCT:



HL60 cell lysate, 200 µg/100 µl, is available for positive



BAFF in human HL60 cell

lysate (A) and murine spleen

tissue lysate (B) with anti-

BAFF (CT) at 1 µg/ml.

REFERENCES:

1. Moore PA, Belvedere O, Orr A, et al. BLyS: member of the

tumor necrosis factor family and B lymphocyte stimulator. Science

1999;285:260-3

2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of

the tumor necrosis factor family, stimulates B cell growth. J Exp

Med 1999;189:1747-56

3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the

TNF family that is down-regulated by mitogens. J Leukoc Biol

1999;65:680-3

4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB.

Identification and characterization of a novel cytokine, THANK, a

TNF homologue that activates apoptosis, nuclear factor-kappaB,

and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81

5. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are

receptors for a TNF homologue implicated in B-cell autoimmune

disease. Nature 2000;404:995-9

6. Khare SD, Sarosi I, Xia XZ, et al. Severe B cell hyperplasia and

autoimmune disease in TALL-1 transgenic mice. Proc Natl Acad

Sci USA 2000;97:3370-5

(WD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-APRIL (ED) TALL-2, TRDL-1

CATALOG No.: 2223

BACKGROUND:




designated APRIL (for a proliferation-inducing ligand), TALL-

2 (for TNF- and ApoL-related Leukocyte-expressed Ligand

2), and TRDL-1α (for TNF related death ligand 1α) in human

and mouse (1-4). Two receptors for APRIL were recently

identified and designated TACI and BCMA (5-7). APRIL

stimulates B and T cell proliferation, triggers humoral

immune responses, activates NF-kB, and induces cell death

(1-7). APRIL and its close relative of BlyS and their

receptors BCMA and TACI are involved in diseases of

autoimmunity and cancer (8,9).

SOURCE:

Rabbit anti-APRIL (ED) polyclonal antibody was raised

against a synthetic peptide (GTGGPSQNGEGYP)

corresponding to amino acids 67 to 79 of human APRIL (1-

3).

APPLICATION:

This antibody can be used for detection of APRIL by

Western blot at 4-5 µg/ml. HL60 or Jurkat cell lysate can be

used as positive control and an approximately 42 kDa band


STORAGE:




RELATED PRODUCT:



REFERENCES:

1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of

the tumor necrosis factor family, stimulates tumor cell growth. J

Exp Med. 1998;188(6):1185-90.

2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the

TNF family that is down-regulated by mitogens. J Leukoc Biol.

1999;65(5):680-3.

3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor

necrosis factor-like ligand, stimulates cell death. Cancer Res .

2000;60(4):1021-7.

4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and

receptors BCMA and TACI: system for regulating humoral

immunity. Nat Immunol. 2000;1(3):252-6.

5. Wu Y, Bressette D, Carrell JA, et a. Tumor necrosis factor

(TNF) receptor superfamily member TACI is a high affinity receptor

for TNF family members APRIL and BLyS. J Biol Chem.

2000;275(45):35478-85.

6. Marsters SA, Yan M, Pitti RM, et al. Interaction of the TNF

homologues BLyS and APRIL with the TNF receptor homologues

BCMA and TACI. Curr Biol. 2000;10(13):785-8.

7. Rennert P, Schneider P, Cachero TG, at al. A soluble form of B

cell maturation antigen, a receptor for the tumor necrosis factor

family member APRIL, inhibits tumor cell growth. J Exp Med.

2000;192(11):1677-84.

8. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are

receptors for a TNF homologue implicated in B-cell autoimmune

disease. Nature 2000;404:995-9

9. Ware CF. APRIL and BAFF connect autoimmunity and cancer. J

Exp Med. 2000;192(11):F35-8.

(WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DEDAF

CATALOG No.: 2227

BACKGROUND:



signals are transduced by death domain (DD) death effector

domain (DED), and caspase recruitment domain (CARD)

containing molecules. Several molecules including caspases

and adaptor FADD contain DEDs. A novel protein that

interacts with DED of caspase-8 and –10, and FADD was

identified recently and designated DEDAF for DED

associated factor (1). DEDAF is identical to the

transcriptional repressor RYBP (2). DEDAF/RYBP is

expressed in multiple tissues and cell lines. DEDAF interacts

with FADD and augments the formation of

CD95/FADD/capase-8 complexes at the cell membrane, and

interacts with DED-containing DNA biding protein (DEDD) in

the nucleus indicating it is involved in the regulation of both

cytoplasmic and nuclear events of apoptosis.

SOURCE:

Rabbit anti-DEDAF polyclonal antibody was raised against a

synthetic peptide (TPKGDMSAVNDESF) corresponding to

amino acids 215 to 228 of human DEDAF (1,2). The

sequence is identical to that of mouse origin (2).

APPLICATION:

This antibody can be used for detection of DEDAF by

Western blot at 0.5 to 1 µg/ml. Human A549 or HepG2 cell

lysate can be used as a positive control. A band at

approximately 32 kDa can be detected. It is human, mouse,

and rat reactive.


STORAGE:




Western blot analysis of DEDAF

expression in human A549

(lane A), HepG2 (lane B), and

mouse 3T3 (lane C) cell lysates

with anti-DEDAF at 1 µg /ml.

RELATED PRODUCTS:



Human HepG2 cell lysate, 200 µg at 2 mg/ml, is available

for positive control (Catalog No. 1211).

REFERENCES:

1. Zheng L, Schickling O, Peter ME, Lenardo MJ. The death

effector domain-associated factor (DEDAF) plays distinct

regulatory roles in the nucleus and cytoplasm. J Biol Chem.

2001;276(34):31945-52.

2. Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC,

Vidal M. RYBP, a new repressor protein that interacts with

components of the mammalian Polycomb complex, and with the

transcription factor YY1. EMBO J. 1999;18(12):3404-18.

(RD0201)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Survivin (NT) TIAP/API4

CATALOG No.: 2233

BACKGROUND:

Apoptosis, or programmed cell death, is related to many

diseases, such as cancer. Apoptosis is triggered by a variety

of stimuli including members in the TNF family and

prevented by the inhibitor of apoptosis (IAP) proteins. IAP

proteins form a conserved gene family that binds to and

inhibits cell death proteases. A novel IAP protein was

recently identified and designated survivin, apoptosis

inhibitor 4 (API4), and TIAP (1-3). Survivin/TIAP interacted

with the processed form of caspase-3 and inhibited its

proteolytic activity. Survivin/TIAP is predominantly expressed

in tissues of embryos, transformed cell lines, and many

human cancers and lymphomas (1).

SOURCE:

Rabbit anti-survivin (NT) polyclonal antibody was raised


human origin (1).

APPLICATION:

This polyclonal antibody can be used for detection of survivin

by Western blot at 0.5 to 1 µg/ml. MOLT4 cell lysate can be


should be detected. For research use only.

STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2233P). MOLT4 cell

lysate, 200 µg at 2 mg/ml, is available for positive control

(Catalog No. 1206).


survivin in MOLT4 cell



peptide with anti-survivin

(NT) at 1 µg/ml.

REFERENCES:

1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,

survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-

21

2. Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC,

Altieri DC. Control of apoptosis and mitotic spindle checkpoint by

survivin. Nature 1998;396:580-4

3. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T.

Expression of a murine homologue of the inhibitor of apoptosis

protein is related to cell proliferation. Proc Natl Acad Sci USA

1999;96:1457-62

(WD0600)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Survivin (CT) TIAP/API4

CATALOG No.: 2235

BACKGROUND:












human cancers and lymphomas (1,3).

SOURCE:

Rabbit anti-survivin (CT) polyclonal antibody was raised



APPLICATION:


by Western blot at 0.5 to 1 µg/ml. MOLT4 cell lysate can be


should be detected. For research use only.

STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2235P). MOLT4 cell


(Catalog No. 1206).


survivin in MOLT4 cell

lysate in the absence (A)

or presence (B) of

blocking peptide with

anti-survivin (CT) at 1

µg/ml.

REFERENCES:



21







1999;96:1457-62

(WD0600)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Survivin (CT) TIAP/API4

CATALOG No.: 2237

BACKGROUND:












human cancers and lymphomas (1,3).

SOURCE:

Rabbit anti-survivin (CT) polyclonal antibody was raised

against a synthetic peptide (AKTTRQSIEQLAA)

corresponding to amino acids 128 to 140 of mouse origin (3).

APPLICATION:


by Western blot at 0.5 to 1 µg/ml. Mouse spleen tissue lysate

can be used as positive control and an approximate 17 kDa

band can be detected. For research use only.

STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2237P). Mouse spleen


(Catalog No. 1406).


survivin in mouse spleen

tissue lysate with anti-survivin

(CT) at 1 µg/ml.

REFERENCES:



21







1999;96:1457-62

(WD1000)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-AIF (NT)

CATALOG No.: 2239

BACKGROUND:





and several novel proteins (1). A novel gene, the product of

which causes chromatin condensation and DNA

fragmentation, was recently identified, cloned, and

designated apoptosis inducing factor (AIF) (2). Like the

critical molecules, cytochrome c and caspase-9, in

apoptosis, AIF localizes in mitochondria. AIF translocates to

the nucleus when apoptosis is induced and induces

mitochondria to release the apoptogenic proteins

cytochrome c and caspase-9. AIF induces chromatin

condensation and DNA fragmentation, which are the

hallmarks of apoptosis, of the isolated nucleus and the

nucleus in live cells by microinjection. AIF is highly

conserved between human and mouse and widely

expressed (2).

SOURCE:

Rabbit anti-AIF (N-terminus of mature AIF) polyclonal

antibody was raised against a peptide corresponding to

amino acids 109 to 122 of human AIF (2).

APPLICATION:

This polyclonal antibody can be used for detection of AIF by

Western blot at 0.25 to 1 µg/ml. K562 cell lysate can be used

as positive control and a 67 kDa band should be detected.


STORAGE:





AIF in K562 cell lysate with

anti-AIF (NT) at 1 µg/ml.

RELATED PRODUCTS:





REFERENCES:

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature

1999;401:127-8

2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular

characterization of mitochondrial apoptosis-inducing factor. Nature

1999;397:441-6

(WD0500)


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Anti-Acinus (NP)

CATALOG No.: 2241

BACKGROUND:















AcinusS’ (2).

SOURCE:

Rabbit anti-Acinus (NP) polyclonal antibody was raised

against a peptide (DDPVRTAQVPSPPRGK) corresponding

to amino acids 994 to 1009 of human AcinusL, 267 to 282 of

human AcinusS’, or 236 to 251 of human AcinusS, which are

identical to those of mouse Acinus (2). The selected

antigenic sequence is located near the N-terminus of active

peptide p17.

APPLICATION:





STORAGE:





Acinus in K562 whole cell

lysate with anti-Acinus (NP)

at 1 µg/ml.

RELATED PRODUCTS:





REFERENCES:


Nature 1999 ;401:127-8.




(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-13 / ERICE

CATALOG No.: 2245

BACKGROUND:


family of cell death receptors and their ligands. Cell death is

finally caused by members of the caspase family of

proteases and caspase activated DNases. A novel member

in the caspase family was recently identified and designated

ERICE (for Evolutionarily Related Interleukin-1 β Converting

Enzyme) and caspase-13 (1). Caspase-13 belongs to the

ICE subfamily of caspases. Overexpression of caspase-13

induces apoptosis. Caspase-13 was activated by caspase-8,

which is a key enzyme in death receptor induced apoptosis.

Caspase-13 is expressed in a variety of human tissues and

cell lines (1).

SOURCE:

Rabbit anti-caspase-13 polyclonal antibody was raised


human Acaspase-13 (1).

APPLICATION:


Western blot at 0.5 to 1 µg/ml. HL60 cell lysate can be used


is human, mouse and rat reactive. For research use only.

STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2245P). HL60 cell lysate,

200 µg at 2 mg/ml, is available for positive control (Catalog

No. 1209).

Western blot analysis of casp-

13 in human HL60 cell lysate

(A), mouse brain (B) and rat

brain (C) tissue lysates with

anti-casp-13 at 1 µg/ml.

REFERENCES:

1. Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable

caspase. J Biol Chem 1998;273:15702-7

(WD0500)


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[email protected]

Anti-BACE2 / Asp1 (NT)

CATALOG No.: 2247

BACKGROUND:

Accumulation of the amyloid-β (Aβ) plaque in the cerebral


disease. Aβ peptide is generated by proteolytic cleavage of

the β−amyloid protein precursor (APP) at β- and γ-sites by

proteases. The long-sought β−secretase was recently

identified by several groups independently and designated

beta-site APP cleaving enzyme (BACE) and aspartyl

protease 2 (Asp2) (1-4). A BACE homolog was recently

cloned and designated BACE2, Asp1, DRAP (for Down

region aspartic protease), and memapsin 1 (4-9). BACE2

also cleaves APP at β-site and at a different site within Aβ

(8). BACE2 locates on chromosome 21q22.3, the so-called

‘Down critical region’, suggesting that BACE2 and Aβ may

also contribute to the pathogenesis of Down syndrome (6,7)

SOURCE:

This rabbit polyclonal antibody was raised against a

synthetic peptide (APTPGPGTPAERHADG) corresponding

to amino acids 44 to 59 of human BACE2 (4).

APPLICATION:

This antibody can be used for detection of BACE2 by

Western blot at 0.5 to 1 µg/ml. Human heart tissue lysate

can be used as positive control. It is human, mouse, and rat


STORAGE:




available.

RELATED PRODUCTS:



Human heart tissue lysate, 200 µg at 2 mg/ml, is available



BACE2 in human (A) and

mouse (B) heart tissue

lysates with anti-BACE2

(CT) at 1 µg/ml.

REFERENCES: 1. Vassar R, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41 2. Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci 1999;14:419-27 3. Sinha S, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999;402:537-40 4. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999;402:533-7 5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc Natl Acad Sci USA 2000 15;97:1456-60 6. Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59-64 7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177-184 8. Farzan M, et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc Natl Acad Sci USA 2000;97:9712-7 9. Bennett BD, et al. Expression analysis of BACE2 in brain and peripheral tissues. J Biol Chem 2000;275:20647-51

(WD0900)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-BACE2 / Asp1 (CT)

CATALOG No.: 2249

BACKGROUND:





proteases. The long-sought β−secretase was recently



protease 2 (Asp2) (1-4). BACE/Asp2 is a novel

transmembrane aspartic protease and co-localizes with

APP. A BACE homolog was recently cloned and designated

BACE2, Asp1, DRAP (for Down region aspartic protease),

and memapsin 1 (4-9). BACE2 also cleaves APP at β-site

and at a different site within Aβ (8). BACE2 locates on

chromosome 21q22.3, the so-called ‘Down critical region’,

suggesting that BACE2 and Aβ may also contribute to the

pathogenesis of Down syndrome (6,7)

SOURCE:

This rabbit polyclonal antibody was raised against a peptide

corresponding to amino acids 496 to 511 of human BACE2

(4).

APPLICATION:

This antibody can be used for detection of BACE2 by

Western blot at 0.5 to 1 µg/ml. Human heart tissue lysate

can be used as positive control. For research use only.

STORAGE:




available.


BACE2 in human heart


(A) or presence (B) of

blocking peptide (2249P)

with anti-BACE2 (CT) at 1

µg/ml.

RELATED PRODUCTS:



Human heart tissue lysate, 200 µg at 2 mg/ml, is available


REFERENCES: 1. Vassar R, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41 2. Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci 1999;14:419-27 3. Sinha S, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999;402:537-40 4. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999;402:533-7 5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc Natl Acad Sci USA 2000 15;97:1456-60 6. Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59-64 7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177-184 8. Farzan M, et al. BACE2, a beta-secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc Natl Acad Sci USA 2000;97:9712-7 9. Bennett BD, et al. Expression analysis of BACE2 in brain and peripheral tissues. J Biol Chem 2000;275:20647-51

(WD0900)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-BACE / Asp2 (CT)

CATALOG No.: 2253

BACKGROUND:





two proteases. APP is first cleaved by β−secretase,

producing a soluble derivative of the protein and a

membrane anchored 99-amino acid carboxy-terminal


γ−secretase to generate the 4 kDa amyloid-β peptide, which

is deposited in the brains of all suffers of Alzheimer’s

disease. The long-sought β−secretase was recently



protease 2 (Asp2) (1-4). BACE/Asp2 is a novel

transmembrane aspartic protease and colocalizes with APP.

SOURCE:

This rabbit polyclonal antibody was raised against a peptide

corresponding to amino acids 485 to 501 of human BACE

(1). The peptide sequence differs from those of mouse and

rat BACE by one amino acid (1).

APPLICATION:

This antibody can be used for detection of BACE by Western

blot at 0.5 to 1 µg/ml. Human brain tissue lysate can be used

as positive control and an approximately 70 kDa band

should be detected. It is human and mouse reactive. For

research use only.

STORAGE:




available.


BACE in human brain tissue



peptide (2253P) and in mouse

3T3 cell lysate (C) with anti-

BACE (CT) at 1 µg/ml.

RELATED PRODUCTS:



Human brain tissue lysate, 200 µg at 2 mg/ml, is available


REFERENCES:

1. Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase

cleavage of Alzheimer's amyloid precursor protein by the

transmembrane aspartic protease BACE. Science 1999;286:735-

41

2. Hussain I, Powell D, Howlett DR, et al. Identification of a novel

aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci

1999;14:419-27

3. Yan R, Bienkowski MJ, Shuck ME, et al. Membrane-anchored

aspartyl protease with Alzheimer's disease beta-secretase activity.

Nature 1999;402:533-7

4. Sinha S, Anderson JP, Barbour R, et al. Purification and cloning

of amyloid precursor protein beta-secretase from human brain.

Nature 1999;402:537-40 (WD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-AIF (IN)

CATALOG No.: 2267

BACKGROUND:














condensation and large scale DNA fragmentation, which are

the hallmarks of apoptosis, of the isolated nucleus and the

nucleus in live cells by microinjection and apoptosis stimuli

(2,3). AIF is highly conserved between human and mouse

and widely expressed (2).

SOURCE:

Rabbit anti-AIF (IN) polyclonal antibody was raised against a


AIF (2). This sequence is identical to those of mouse and rat

AIF (2).

APPLICATION:




is human, mouse and rat reactive. For research use only.

Western blot analysis of AIF

in K562 cell lysate (A), rat

heart (B), and mouse heart

(C) tissue lysates with anti-

AIF (IN) at 1 µg/ml.

STORAGE:




RELATED PRODUCTS:





REFERENCES:


1999;401:127-8



1999;397:441-6

3. Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear

translocation of AIF in apoptosis and necrosis. FASEB J

2000;14:729-39

(WD0500)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-FLASH (CT)

CATALOG No.: 2271

BACKGROUND:

A novel mammalian CED-4 homologous was recently

identified and cloned in mouse (1) and human (2) and

designated FLASH (for FLICE-associated huge protein).

FLASH is involved in Fas induced apoptosis. It is recruited to

Fas after the receptor cross-linking. Overexpression of wild

type of FLASH facilitates and its dominant negative form

inhibits Fas induced apoptosis. FLASH interacts with the

DEDs of caspase-8 and FADD through the DED-like domain

of FLASH and mediates activation of caspase-8 (1). There

are parallels between FLASH and Apaf-1/CED-4 although

there are arguments against their structural similarity (1,2).

FLASH is widely expressed.

SOURCE:

Rabbit anti-FLASH (CT) polyclonal antibody was raised

against a synthetic peptide (SERFQQLMKLFEKSKC)

corresponding to amino acids 1253 to 1268 of human origin,

which differ from those of mouse by one amino acid (1,2).

APPLICATION:

This polyclonal antibody can be used for detection of FLASH

by Western blot at 0.25 to 0.5 µg/ml. HeLa cell lysate can be



STORAGE:





FLASH in HeLa whole cell

lysate with anti-FLASH (CT)

at 0.5 µg/ml.

RELATED PRODUCTS:



HeLa cell lysate, 200 µg at 2 mg/ml, is available for positive


REFERENCES:

1. Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K,

Yonehara S. The CED-4-homologous protein FLASH is involved in

Fas-mediated activation of caspase-8 during apoptosis. Nature

1999;398:777-85

2. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM

Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3

3. Medema JP. Apoptosis. Life and death in a FLASH. Nature

1999;398:756-7

(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-F1Aα

CATALOG No.: 2279

BACKGROUND:

Fas and tumor necrosis factor receptor 1 (TNFR1) are two

prototype members in the death receptor family. A novel

protein that associates with the intracellular domains of Fas

and TNFR1 was recently identified and designated

F1Aα and FEM1β (1,2). F1Aα/FEM1β is the homologue of C.

elegans sex determining protein FEM-1 (1,2). FEM-

1/F1Aα is cleaved by CED-3 and caspase (3). FEM-

1/F1Aα associates with CED-4 and its mammalian

homologue Apaf-1 (3). Overexpression of F1Aα induces

apoptosis. F1Aα is therefore a novel member of the death

receptor associated protein that mediates apoptosis. F1Aα

is expressed in a variety of human and mouse tissues (1,2)

SOURCE:

Rabbit anti-F1Aα polyclonal antibody was raised against a

peptide (DINYQDQIPRTLEE) corresponding to amino acids

609 to 622 of human F1Aα (1). This sequence is identical to

the corresponding sequence of mouse FEM1β (2).

APPLICATION:

This polyclonal antibody can be used for detection of F1Aα

by Western blot at 0.5 to 1 µg/ml. Mouse liver tissue lysate

can be used as positive control and an approximately 70 kDa

band can be detected. It is human, mouse, and rat reactive.

For research use only. STORAGE:




RELATED PRODUCTS:



Mouse liver tissue lysate, 200 µg at 2 mg/ml, is available for


Western blot analysis of F1Aα

in mouse (A) and rat (B) liver

tissue lysates with anti- F1Aα

at 1 µg /ml.

REFERENCES:

1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu

VC. F1Aalpha, a death receptor-binding protein homologous to the

Caenorhabditis elegans sex-determining protein, FEM-1, is a

caspase substrate that mediates apoptosis. J Biol Chem.

1999;274(45):32461-8.

2. Ventura-Holman T, Maher JF. Sequence, organization, and

expression of the human FEM1B gene. Biochem Biophys Res

Commun 2000;267(1):317-20

3. Chan SL, Yee KS, Tan KM, Yu VC. The Caenorhabditis elegans

sex determination protein FEM-1 is a CED-3 substrate that

associates with CED-4 and mediates apoptosis in mammalian

cells. J Biol Chem. 2000;275(24):17925-8.

(WD0102)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-RIP3

CATALOG No.: 2283

BACKGROUND:

Certain serine/threonine protein kinases, such as ASK1, RIP,

DAP, and ZIP kinases, are mediators of apoptosis. Receptor

interacting proteins including RIP and RIP2/RICK mediate

apoptosis induced by TNFR1 and Fas, two prototype

members in the death receptor family. A novel member in

the RIP kinase family was recently identified and designated

RIP3 (1-3). RIP3 contains N-terminal kinase domain but,

unlike RIP or RIP2, lacks the C-terminal death or CARD

domain. RIP3 binds to RIP and TNFR1, mediates TNFR1

induced apoptosis, and attenuates RIP and TNFR1 induced

NF-κB activation. Overexpression of RIP3 induces apoptosis

and NF-κB activation. The messenger RNA of RIP3 is

expressed in a subset of adult tissues (2,3).

SOURCE:

Rabbit anti-RIP3 polyclonal antibody was raised against a

peptide (AQFGRGRGWQPFHK) corresponding to amino

acids 473 to 486 of murine RIP3 (3).

APPLICATION:

This polyclonal antibody can be used for detection of RIP3

by Western blot at 0.5 to 1 µg/ml. 3T3 cell lysate can be


can be detected. It is mouse and rat reactive. For research

use only. STORAGE:




RELATED PRODUCTS:



Mouse 3T3 whole cell lysate, 200 µg at 2 mg/ml, is

available for positive control (Catalog No. 1212).

Western blot analysis of RIP3 in

mouse 3T3 whole cell lysate in

the absence (A) or presence (B)

of blocking peptide with anti-

RIP3 at 1 µg /ml.

REFERENCES:

1. Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP,

Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that

activates apoptosis and NFkappaB. Curr Biol. 1999;9(10):539-42.

2. Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3,

a novel apoptosis-inducing kinase. J Biol Chem.

1999;274(24):16871-5.

3. Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse

receptor interacting protein 3 does not contain a caspase-recruiting

or a death domain but induces apoptosis and activates NF-

kappaB. Mol Cell Bio. 1999; 19(10):6500-8

(WD0102)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ASC / TMS1

CATALOG No.: 2287

BACKGROUND:

Apoptosis is regulated by death domain (DD) and/or caspase


caspase family of proteases. CARD containing cell death

regulators include RAIDD, RICK, BCL10, Apaf-1, ARC,

caspase-9, and caspase-2. A novel CARD domain

containing protein was recently identified in human and

mouse and designated ASC and TMS1 (1-3). Ectopic

expression of ASC/TMS1 induced apoptosis through

activation of caspase-9 and inhibited the survival of human

breast cancer cells (3,4). Overexpression of ASC/TMS1

induced DNA fragmentation (4). ASC/TMS1 is expressed in

a variety of human and mouse tissues (1, 2)

SOURCE:

Rabbit anti-ASC/TMS1 polyclonal antibody was raised

against a synthetic peptide (RESQSYLVEDLERS)

corresponding to amino acids 182 to 195 of human ASC (1).

APPLICATION:


ASC/TMS1 by Western blot at 1 µg/ml. HL60 whole cell


25 kDa band can be detected. It is human reactive. For

research use only. STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2287P). HL60 whole cell


(Catalog No. 1209).

Western blot analysis of ASC in

HL60 whole cell lysate in the


blocking peptide (2287P) with anti-

ASC at 1 µg /ml.

REFERENCES:

1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T,

Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a

novel 22-kDa protein, aggregates during apoptosis of human

promyelocytic leukemia HL-60 cells. J Biol Chem.

1999;274(48):33835-8.

2. Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J.

Murine Ortholog of ASC, a CARD-Containing Protein, Self-

Associates and Exhibits Restricted Distribution in Developing

Mouse Embryos. Exp Cell Res. 2001;262(2):128-133.

3. Conway KE, McConnell BB, Bowring CE, Donald CD, Warren

ST, Vertino PM. TMS1, a novel proapoptotic caspase recruitment

domain protein, is a target of methylation-induced gene silencing in

human breast cancers. Cancer Res. 2000;60(22):6236-42.

4. McConnell BB, Vertino PM. Activation of a caspase-9-mediated

apoptotic pathway by subcellular redistribution of the novel

caspase recruitment domain protein TMS1. Cancer Res.

2000;60(22):6243-7.

(WD0102)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bnip3L (IN) Bnip3α, Nix

CATALOG No.: 2289

BACKGROUND:



homology 3 (BH3) domain is a potent death domain. BH3

domain containing pro-apoptotic proteins, including Bad,

Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass

of the Bcl-2 family. A novel BH3 domain containing protein

was recently identified and designated Bnip3L, Bnip3α, and

Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3α/Nix is a

homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic

protein Bnip3. Overexpression of Bnip3L induces apoptosis

(2,3). Bnip3L interacts with and overcomes suppresses by

Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The

messenger RNA of Bnip3L is ubiquitously expressed in

human tissues (1,2). Bnip3L and Bnip3 form a new subfamily

of the pro-apoptotic mitochondrial proteins.

SOURCE:

Rabbit anti-Bnip3L (IN) polyclonal antibody was raised

against a peptide (DAQHESGQSSSRGSSH) corresponding

to amino acids 77 to 92 of human origin, which are identical

to those of mouse Bnip3L (3).

APPLICATION:

This polyclonal antibody can be used for detection of Bnip3L


used as positive control and the approximate 40 kDa top

band represents monomer of Bnip3L (4). It is human and


STORAGE:




Western blot analysis of Bnip3L

in K562 whole cell lysate in the

absence (A), or presence (B) of

immunogenic peptide (2289P)

with anti-Bnip3L (IN) at 1 µg/ml.

RELATED PRODUCTS:





REFERENCES:

1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation,

mapping, and functional analysis of a novel human cDNA (BNIP3L)

encoding a protein homologous to human NIP3. Genes

Chromosomes Cancer 1998;21:230-5

2. Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G.

BNIP3alpha: a human homolog of mitochondrial proapoptotic

protein BNIP3. Cancer Res 1999;59:533-7

3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a

subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem

1999;274(1):7-10

4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-

interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL

and induces apoptosis by altering mitochondrial membrane

permeability. Oncogene. 1999;18:4523-9 (RD1200)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DcR1 (ED2) TRAIL-R3, TRID, LIT

CATALOG No.: 2299

BACKGROUND:






receptors for TRAIL (1-3). Two decoy receptors for TRAIL

have been identified and designated DcR1/TRID/TRAIL-

R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1

has extracellular TRAIL-binding domain but lacks

intracellular signaling domain. It is a glycophospholipid-

anchored cell surface protein. DcR1 transcripts are

expressed in many normal human tissues but not in most

cancer cell lines (2,3). Overexpression of DcR1 did not

induce apoptosis, but attenuated TRAIL-induced apoptosis

(2,3).

SOURCE:

Rabbit anti-DcR1 (ED2) polyclonal antibody was raised

against a peptide (CKEGTFRNENSPE) corresponding to

amino acids 111 to 123 at extracellular domain of human

DcR1 precursor (2-3).

APPLICATION:


by Western blot at 0.5 to 1 µg/ml. HeLa cell lysate can be


can be detected (6). It is human, mouse, and rat reactive.


STORAGE:

It is supplied as chromatography purified IgG, 100 µg in 200



RELATED PRODUCTS:



HeLa cell lysate, 200 µg at 2 mg/ml, is available for positive


Western blot analysis of DcR1 in

HeLa cell (A), mouse (B) and rat (C)

liver tissue lysates with anti-DcR1

(ED2) at 1 µg/ml.

REFERENCES: 1. Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic

ligand TRAIL. Science; 1997;276:111-113

2. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death

domain-containing receptor for TRAIL. Science 1997;277:815-8

3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced


1997;277:818-21

4. Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and

characterization of TRAIL-R3, a novel member of the emerging TRAIL

receptor family. J Exp Med 1997;186(7):1165-70

5. MacFarlane M, Ahmad M, Srinivasula SM, et al. Identification and

molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J

Biol Chem 1997;272(41):25417-20

6. Schneider P, Bodmer JL, Thome M, Hofmann K, Holler N, Tschopp J.

Characterization of two receptors for TRAIL. FEBS Lett 1997;416(3):329-34

7. Mongkolsapaya J, Cowper AE, Xu XN, et al. Lymphocyte inhibitor of

TRAIL (TNF-related apoptosis -inducing ligand): a new receptor protecting

lymphocytes from the death ligand TRAIL. J Immunol 1998;160(1):3-6

8. Marsters SA, Sheridan JP, Pitti RM, et al. A novel receptor for

Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6

9. Degli-Esposti MA, Dougall WC, Smolak PJ, et al. The novel receptor

TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated

apoptosis, yet retains an incomplete death domain. Immunity 1997;7:813-20

10. Pan G, Ni J, Yu G, et al. TRUNDD, a new member of the TRAIL

receptor family that antagonizes TRAIL signalling. FEBS Lett 1998;424:41-5

(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-AIF (CT)

CATALOG No.: 2301

BACKGROUND:














condensation and DNA fragmentation, which are the

hallmarks of apoptosis, of the isolated nucleus and the

nucleus in live cells by microinjection. AIF is highly

conserved between human and mouse and widely

expressed (2).

SOURCE:

Rabbit anti-AIF (CT) polyclonal antibody was raised against

a peptide (KDGEQHEDLNEVAK) corresponding to amino

acids 593 to 606 of human AIF (2). This sequence is

identical to those of mouse and rat AIF (2).

APPLICATION:




is human, mouse, and rat reactive. For research use only.

STORAGE:




Western blot analysis of AIF in

K562 cell lysate (A), mouse (B),

and rat (C) liver tissue lysates

with anti-AIF (CT) at 1 µg/ml.

RELATED PRODUCTS:





REFERENCES:


1999;401:127-8



1999;397:441-6

(WD0800)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DAP Kinase 2

CATALOG No.: 2323

BACKGROUND:



serine/threonine protein kinases, such as RIP and DAP

kinase, are mediators of apoptosis. DAP kinase (DAPK) is

pro-apoptotic calcium-regulated serine/threonine kinase

containing death domain. Ectopic expression of DAPK

induces cell death and suppresses oncogenic

transformation. DAPK mediates IFNγ induced apoptosis. A

novel DAP kinase-related protein was recently identified and

designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed

DAPK2 induced apoptosis in various types of cells (1,2).

DAPK has high sequence homology to ZIP kinase and

DRAK1/2, and they represent a novel family of

serine/threonine kinases, which mediates apoptosis through

their catalytic activities. The messenger RNA of DAPK2 is

expressed in multiple human tissues (1).

SOURCE:

Rabbit anti-DAPK2 polyclonal antibody was raised against a

peptide (ARRKALHPRRRSSTS) corresponding to amino

acids 356 to 370 of human DAPK2 (1,2). The sequence of

this antigenic peptide is identical to the corresponding amino

acids of mouse origin (1,2)

APPLICATION:

This polyclonal antibody can be used for detection of DAPK2

by Western blot at 0.5 to 1 µg/ml. A431 whole cell lysate can


band can be detected. It is human, mouse, and rat reactive,

and has no cross responses to DAPK1. For research use

only.

STORAGE:





DAPK2 in A431 (H), mouse

spleen (M), and rat kidney (R)

lysates with anti-DAPK2

(2323) at 1 µg/ml.

RELATED PRODUCTS:



A431 cell lysate, 200 µg at 2 mg/ml, is available for positive


REFERENCES:

1. Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ,

Jenkins NA, Akira S. Death-associated protein kinase 2 is a new

calcium/calmodulin-dependent protein kinase that signals

apoptosis through its catalytic activity. Oncogene

1999;18(23):3471-80

2. Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-

associated protein kinase-related protein 1, a novel

serine/threonine kinase involved in apoptosis. Mol Cell Biol

2000;20(3):1044-54

(WD0101)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-12 (NT)

CATALOG No.: 2325

BACKGROUND:

Three distinct signaling pathways lead to programmed cell

death (apoptosis). The death receptor and mitochondrion

pathways are the mains, in which the key apoptotic

proteases capase-8 and caspase-9, respectively, are

involved. The endoplasmic reticulum (ER) stress is the third

apoptotic pathway and caspase-12 is involved (1,2).

Caspase-12 is localized to the ER but not to cytoplasm or

mitochondrion. Caspase-12 is activated by ER stress,

including disruption of ER calcium homeostasis, and

mediates ER stress-induced apoptosis. Caspase-12 is co-

localized to the ER with several proteins that are involved in

Alzheimer’s disease including γ-secretase presenilin and β-

amyloid precursor protein (APP). Caspase-12 mediates

cytotoxicity induced by amyloid-β. Caspase-12 is

ubiquitously expressed in mouse tissues (1).

SOURCE:

Rabbit anti-caspase-12 (NT) polyclonal antibody was raised

against a peptide (AARRTHERDPIYKIKG) corresponding

to amino acids 2 to 17 of murine caspase-12 (3).

APPLICATION:


caspase-12 by Western blot at 0.5 to 1 µg/ml. Murine spleen

tissue lysate can be used as positive control. It is human,


STORAGE:





caspase-12 in human (A)

and mouse (B) spleen

tissue lysates with anti-

caspase-12 (NT) at 1 µg/ml.

RELATED PRODUCTS:



Murine spleen tissue lysate, 200 µg at 2 mg/ml, is available


REFERENCES:

1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA,

Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific

apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-

103.

2. Mehmet H. Caspases find a new place to hide. Nature

2000;403:29-30

3. Van de Craen M, Vandenabeele P, Declercq W, Van den

Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W,

Beyaert R, Fiers W. Characterization of seven murine caspase

family members. FEBS Lett 1997;403:61-9

(WD1000)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Caspase-12 (IN)

CATALOG No.: 2327

BACKGROUND:

Three distinct signaling pathways lead to programmed cell

death (apoptosis). The death receptor and mitochondrion

pathways are the mains, in which the key apoptotic

proteases capase-8 and caspase-9, respectively, are

involved. The endoplasmic reticulum (ER) stress is the third

apoptotic pathway and caspase-12 is involved (1,2).

Caspase-12 is localized to the ER but not to cytoplasm or

mitochondrion. Caspase-12 is activated by ER stress,

including disruption of ER calcium homeostasis, and

mediates ER stress-induced apoptosis. Caspase-12 is co-

localized to the ER with several proteins that are involved in

Alzheimer’s disease including γ-secretase presenilin and β-

amyloid precursor protein (APP). Caspase-12 mediates

cytotoxicity induced by amyloid-β. Caspase-12 is

ubiquitously expressed in mouse tissues (1).

SOURCE:

Rabbit anti-caspase-12 (IN) polyclonal antibody was raised

against a peptide (CTPSSPSESKRKVEDDE) corresponding

to amino acids 100 to 116 of murine caspase-12 (3).

APPLICATION:


caspase-12 by Western blot at 0.5 to 1 µg/ml. Murine brain

tissue lysate can be used as positive control. It is human,


STORAGE:





caspase-12 in murine brain


(A) or presence (B) of

blocking peptide with anti-

caspase-12 (IN) at 1 µg/ml.

RELATED PRODUCTS:



Murine brain tissue lysate, 200 µg at 2 mg/ml, is available


REFERENCES:

1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA,

Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific

apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-

103.

2. Mehmet H. Caspases find a new place to hide. Nature

2000;403:29-30

3. Van de Craen M, Vandenabeele P, Declercq W, Van den

Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W,

Beyaert R, Fiers W. Characterization of seven murine caspase

family members. FEBS Lett 1997;403:61-9

(WD0900)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKε / IKK-i (CT)

CATALOG No.: 2329

BACKGROUND:





response to extracellular stimuli. NF-κB is associated with

IκB proteins in the cell cytoplasm, which inhibit NF-κB

activity. IκB is phosphorylated by IκB kinase (IKK) complex

that contains IKKα, IKKβ, and IKKγ. A novel molecule in the

IKK complex was recently identified and designated

IKKε /IKK-i (1,2). IKKε is required for the activation of NF-κB

by PMA and T cell receptors but not by TNFα and IL-1 (1).

IKKε /IKK-i message is expressed in a variety of tissues and

is inducible by TNFα, IL-1, and LPS (2).

SOURCE:

Rabbit anti-IKKε polyclonal antibody was raised against a

peptide (NRIIERLNRVPAPPDV) corresponding to amino

acids 701 to 716 of human IKKε /IKK-i (1,2).

APPLICATION:

This polyclonal antibody can be used for detection of IKKε by

Western blot at 0.5 to 1 µg/ml. Jurkat whole cell lysate can

be used as positive control and an 80 kDa band should be

detected. It has no cross response to IKKα, IKKβ, or IKKγ.


STORAGE:





IKKε in Jurkat whole cell

lysate with anti-IKKε /IKK- i

at 1 µg/ml.

RELATED PRODUCTS:



REFERENCES:

1. Peters RT, Liao SM, Maniatis T. IKKepsilon is part of a novel

PMA-inducible IkappaB kinase complex. Mol Cell 2000;5(3):513-22

2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi

Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharide-

inducible kinase that is related to IkappaB kinases. Int Immunol

1999;11(8):1357-62

(WD0101)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IKKγ / NEMO (NT)

CATALOG No.: 2335

BACKGROUND:







activity. The IκB kinase (IKKα and IKKβ) phosphorylates IκB

and mediates NF-κB activation. A novel molecule in the IKK

complex was recently identified and termed

IKKγ/NEMO/FIP3/IKKAP1 (1-5). IKKγ interacts with IKKβ and

is required for the activation of IKK complex and NF-κB by

LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also

shown to bind to RIP and NIK and to mediate TNF-induced

NF-κB activation (3).

SOURCE:

Rabbit anti-IKKγ polyclonal antibody was raised against a

peptide (CQYQAPDMDTLQIHVME) corresponding to amino

acids 400 to 416 of human IKKγ (1,2), which are identical to

those of mouse homologue (2).

APPLICATION:

This polyclonal antibody can be used for detection of IKKγ by

Western blot at 0.5 to 1 µg/ml. HeLa whole cell lysate can be

used as positive control and a 52 kDa band should be

detected. It is human, mouse, and rat reactive and has no

cross response to IKKα or IKKβ. For research use only.

STORAGE:




RELATED PRODUCTS:



Western blot analysis of IKKγ


anti-IKKγ/NEMO at 1 µg/ml.

REFERENCES:

1. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an

essential regulatory subunit of the IkappaB kinase complex. Nature

1998;395(6699):297-300

2. Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou

F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a

component of the IkappaB kinase complex essential for NF-

kappaB activation. Cell. 1998;93(7):1231-40.

3. Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A,

Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a

modulator of NF-kappaB activity and as a target of an adenovirus

inhibitor of tumor necrosis factor alpha-induced apoptosis. Proc

Natl Acad Sci U S A 1999;96(3):1042-7

4. Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB,

Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM.

IkappaB kinase (IKK)-associated protein 1, a common component

of the heterogeneous IKK complex. Mol Cell Biol.

1999;19(2):1526-38.

5. Jin DY, Jeang KT. Isolation of full-length cDNA and

chromosomal localization of human NF-kappaB modulator NEMO

to Xq28. J Biomed Sci 1999;6(2):115-20

(WD0101)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DC-SIGN (CT)

CATALOG No.: 2347

BACKGROUND:

Dendritic cells (DCs) that control immune responses were

recently found to capture and transport HIV from the

mucosal area to remote lymph nodes (1), where DCs hand

over HIV to CD4+ T lymphocytes. DCs also amplify the

amount of virus and extend the duration of viral infectivity.

Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-

SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A

DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-

SIGN2) was identified recently (4-8). DC-SIGN forms a novel

gene family with DC-SIGNR and many alternatively spliced

isoforms of DC-SIGN and DC-SIGNR (8). The expression of

DC-SIGN was found in mucosal tissues including placenta,

small intestine, and rectum.

SOURCE:

Rabbit anti-DC-SIGN polyclonal antibody was raised against

a synthetic peptide (CSRDEEQFLSPAPATPN PPPA)

corresponding to amino acids 384 to 404 of human DC-

DIGN (1).

APPLICATION:

This antibody can be used for detection of DC-SIGN by

Western blot at 1 to 2 µg/ml. Human placenta lysate can be

used as a positive control. A band at approximately 44 kDa

can be detected.


STORAGE:

It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl


for one year.

RELATED PRODUCTS:



Human placenta lysate, 200 µg at 2 mg/ml, is available for


Western blot detection of

GST-DC-SIGN fusion

protein expressed in E. Coli

in the absence (lane 1) and

presence (lane 2) of

inducing reagent IPTG with

anti-DC-SIGN at 0.5 µg /ml.

REFERENCES: 1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV -1-binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97. 2. Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J Virol. 2001;75(10):4664-72. 3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000;100(5):575-85. 4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol. 2000;165(6):2937-42. 5. Pohlmann S, Soilleux EJ, Baribaud F, et al DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans. Proc Natl Acad Sci USA. 2001;98(5):2670-2675. 6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV -1 infection. J Exp Med. 2001;193(6):671-8. 7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR: Subunit organisation and binding to multivalent ligands. J Biol Chem. 2001 in press 8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms: Inter-individual variation in expression of DC-SIGN transcripts. J Biol Chem. 2001 in press (WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-DC-SIGN (ED)

CATALOG No.: 2349

BACKGROUND:

Dendritic cells (DCs) that control immune responses were

recently found to capture and transport HIV from the

mucosal area to remote lymph nodes (1), where DCs hand

over HIV to CD4+ T lymphocytes. DCs also amplify the

amount of virus and extend the duration of viral infectivity.

Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-

SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A

DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-

SIGN2) was identified recently (4-8). DC-SIGN forms a novel

gene family with DC-SIGNR and many alternatively spliced

isoforms of DC-SIGN and DC-SIGNR (8). The expression of

DC-SIGN was found in mucosal tissues including placenta,

small intestine, and rectum.

SOURCE:

Rabbit anti-DC-SIGN polyclonal antibody was raised against

a synthetic peptide (CYFMSNSQRN WHDSITA)

corresponding to amino acids 277 to 293 of human DC-

DIGN (1).

APPLICATION:

This antibody can be used for detection of DC-SIGN by

Western blot at 1 to 2 µg/ml. Human placenta lysate can be


can be detected.


STORAGE:



for one year.

RELATED PRODUCTS:



Human placenta lysate, 200 µg at 2 mg/ml, is available for


Western blot analysis of DC-

SIGN expression in human

placenta tissue lysate in the

absence (lane 1) and presence

(lane 2) of blocking peptide with

anti-DC-SIGN at 2 µg /ml.

REFERENCES: 1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV -1-binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97. 2. Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J Virol. 2001;75(10):4664-72. 3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000;100(5):575-85. 4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol. 2000;165(6):2937-42. 5. Pohlmann S, Soilleux EJ, Baribaud F, et al DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans. Proc Natl Acad Sci USA. 2001;98(5):2670-2675. 6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV -1 infection. J Exp Med. 2001;193(6):671-8. 7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR: Subunit organisation and binding to multivalent ligands. J Biol Chem. 2001 in press 8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms: Inter-individual variation in expression of DC-SIGN transcripts. J Biol Chem. 2001 in press (WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-NAK / TBK1 (CT)

CATALOG No.: 2351

BACKGROUND:







activity. Phosphorylation of I-κB by IκB kinase (IKK) complex

leads to degradation of I-κB and activation of NF-κB. The

IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK

related kinase was recently identified and designated TBK1

(TANK-binding kinase 1), NAK (NF-κB-activating kinase),

and T2K (1-3). NAK/TBK1 activates IKKβ through direct

phosphorylation. NAK/TBK1 is activated by growth factors

and PMA and mediates IKK and NF-kB activation in

response to growth factors (2). NAK/TBK1 functions

upstream of NIK and the IKK complex (1,2). NAK/TBK1 is

also critical in protecting embryonic liver from apoptosis (3).

SOURCE:

Rabbit anti-NAK/TBK1 polyclonal antibody was raised

against a synthetic peptide (ERFGSLTMDGGLRNVD)

corresponding to amino acids 712 to 727 of human

NAK/TBK1 (1,2), which are identical to those of mouse

homologue (1).

APPLICATION:


NAK/TBK1 by Western blot at 0.5 to 1 µg/ml. MOLT4 whole

cell lysate can be used as positive control and an 84 kDa

band should be detected. It is human, mouse and rat

reactive and has no cross response to IKKα, IKKβ, IKKγ, or

IKKε . For research use only.

STORAGE:




RELATED PRODUCTS:



REFERENCES:

1. Pomerantz JL, Baltimore D. NF-kappaB activation by a

signaling complex containing TRAF2, TANK and TBK1, a novel

IKK-related kinase. EMBO J 1999;18(23):6694-704

2. Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S,

Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin

M, Nakanishi M. NAK is an IkappaB kinase-activating kinase.

Nature 2000;404(6779):778-82

3. Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie

A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W,

Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic

liver degeneration and impaired NF-kappaB-dependent gene

transcription. EMBO J. 2000;19(18):4976-85.

(WD0101)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IRAK-M

CATALOG No.: 2355

BACKGROUND:

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces

cellular response through IL-1 receptor (IL-1R) and Toll like

receptors (TLR). IL-1 receptor-associated kinase (IRAK and

IRAK2) mediates the activation of NF-κB by IL-1/Toll

receptors (1,2), which is a pivotal transcription factor

mediating inflammatory and immune response. A novel

member in the IRAK/Pelle family was recently identified and

designated IRAK-M (3). IRAKs associate with IL-1/Toll

receptors after IL-1 or LPS stimulation and the dominant

negative mutants of IRAKs inhibit IL-1 or LPS induced NF-

κB activation. Members in IRAK/Pelle family play a central

role in IL-1R/TLR mediated inflammatory responses to

cytokine IL-1 and LPS.

SOURCE:

Rabbit anti-IRAK-M polyclonal antibody was raised against a

peptide (CSSKFSWDEYEQYKKE) corresponding to amino

acids 581 to 596 of human IRAK-M (3).

APPLICATION:

This polyclonal antibody can be used for detection of IRAK-

M by Western blot at 0.5 to 1 µg/ml. Mouse spleen or rat

kidney tissue lysate can be used as positive control and a 68

kDa major band can be detected. It is human, mouse, and

rat reactive. Anti-IRAK-M has no cross response to IRAK or

IRAK2. For research use only.

STORAGE:

It is supplied as chromatography purified IgG, 100 µg in

200 µl of PBS containing 0.02% sodium azide. Store at


RELATED PRODUCTS:



Murine spleen tissue lysate, 200 µg at 2 mg/ml, is available



IRAK-M in mouse spleen (M)

and rat liver (R) tissue lysates

with anti-IRAK-M at 1 µg /ml.

REFERENCES:

1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the




Science 1997;278:1612-5

3. Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z. IRAK-

M is a novel member of the Pelle/interleukin-1 receptor-associated

kinase (IRAK) family. J Biol Chem. 1999;274(27):19403-10.

(WD0101)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-p53R2 (NT)

CATALOG No.: 2383

BACKGROUND:

The p53 tumor-suppressor gene integrates numerous

signals that control cell life and death. Several novel

molecules involved in p53 signaling, including p53R2 (1),

Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2

(6), were recently discovered. p53R2 is a p53 inducible gene

that contains a p53 binding sequence and encodes a subunit

of the enzyme ribonucleotide reductase (1,7). p53R2 is

induced by the reagents, ultraviolet and γ-irradiation that

cause DNA damages. The product of p53R2 gene is directly

involved in the p53 checkpoint for repair of damaged DNA.

The isoform of the p53 family member p73 also induces

p53R2 expression (8). p53R2 is an important target of p53

for tumour suppression.

SOURCE:

Rabbit anti-p53R2 polyclonal antibody was raised against a

synthetic peptide (GDPERPEAAGLDQDER) corresponding

to amino acids 2 to 17 of human p53R2 (1).

APPLICATION:

This antibody can be used for detection of p53R2 by

Western blot at 0.5 to 1 µg/ml. A431 or 3T3 cell lysate can

be used as positive control and a 39 kDa band can be

detected. It is human, mouse, and rat reactive.


STORAGE:




RELATED PRODUCTS:





Western blot analysis of p53R2

expression in A431 (A) and 3T3

cell lysates in the absence (B) or

presence (C) of blocking peptide

with anti-p53R2 at 1 µg /ml.

REFERENCES: 1. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K,

Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-

dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.

2. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle

regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.

3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T,

Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and

candidate mediator of p53-induced apoptosis. Science.

2000;288(5468):1053-8.

4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori

H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator

of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated

p53. Cell. 2000 Sep 15;102(6):8 49-62.

5. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its

effect on cell growth and apoptosis.

Nature. 2000;408:377-81.

6. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein,

is induced by p53 and promotes apoptos is. Nat Genet. 2000;26:122-7.

7. Lozano G, Elledge SJ. p53 sends nucleotides to repair DNA. Nature.

2000;404:24-5.

8. Nakano K, Balint E, Ashcroft M, Vousden KH. A ribonucleotide reductase

gene is a transcriptional target of p53 and p73. Oncogene.

2000;19(37):4283-9. (WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Chk2 (NT)

CATALOG No.: 2391

BACKGROUND:



molecules involved in p53 signaling, including Chk2 (1),

p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2

(6), were recently discovered. The checkpoint kinase Chk2 is

the mammalian homologue of yeast Cds1/Rad53. In

response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly

phosphorylates and activates p53 (7,8). Chk2 serves as

ATM downstream effector to mediate activation of p53. Chk2

also phosphorylates and activates BRCA1, the product of a

tumor suppressor gene that is mutated in breast and ovarian cancer (9).

SOURCE:

Rabbit anti-Chk2 polyclonal antibody was raised against a

synthetic peptide (SRESDVEAQQSHGSSAC) corresponding

to amino acids 2 to 18 of human Chk2 (1).

APPLICATION:

This antibody can be used for detection of Chk2 by Western

blot at 0.5 to 1 µg/ml. Jurkat or K562 cell lysate can be used

as positive control and a 60 kDa band can be detected. It is

human, mouse, and rat reactive.


STORAGE:




RELATED PRODUCTS:



Jurkat cell lysate, 200 µg at 2 mg/ml, is available for



Chk2 expression in K562

(A), Jurkat (B), and HL-60

(C) whole cell lysates with

anti-Chk2 at 1 µg /ml.

REFERENCES: 1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7. 2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9. 3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8. 4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62. 5. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature. 2000;408:377-81. 6. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis. Nat Genet. 2000;26:122-7. 7. Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge SJ. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro.Proc Natl Acad Sci USA. 2000 ;97:10389-94. 8. Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J, Yoshida H, Liu D, Elledge SJ, Mak TW. DNA damage-induced activation of p53 by the checkpoint kinase Chk2. Science. 2000;287:1824-7 9. Lee JS, Collins KM, Brown AL, Lee CH, Chung JH. hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response. Nature. 2000;404:201-4.

(WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Smac / DIABLO (CT)

CATALOG No.: 2409

BACKGROUND:

The inhibitor of apoptosis proteins (IAPs) regulate

programmed cell death by inhibiting members of the caspase

family of enzymes. A novel mammalian protein that binds to

IAPs and neutralizes the inhibitory effect of IAPs on

caspases was recently identified and designated

Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial

protein that is released along with cytochrome c during

apoptosis and activates cytochrome c/Apaf-1/capase-9

pathway. Analysis of the structural basis of Smac/DIABLO

reveals that the N-terminal amino acids are required for

binding of Smac/DIABLO to IAPs and activation of caspases

(3-6). Smac/DIABLO is expressed in a variety of human and

mouse tissues (1,2).

SOURCE:

Rabbit anti-Smac/DIABLO (CT) polyclonal antibody was

raised against a peptide (EERAESEQEAYLRED)


Smac/DIABLO (1).

APPLICATION:


Smac/DIABLO by Western blot at 0.5 to 1 µg/ml. Human

heart tissue lysate can be used as positive control and a 25

kDa band can be detected. It is human, mouse, and rat

reactive. For research use only. STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2409P). Human heart tissue


(Catalog No. 1301).

Western blot analysis of Smac

in human heart tissue lysate in

the absence (A) or presence (B)

of blocking peptide (2409P) with

anti-Smac at 1 µg /ml.

REFERENCES: 1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein

that promotes cytochrome c-dependent caspase activation by

eliminating IAP inhibition. Cell. 2000;102(1):33-42.

2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid

GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a

mammalian protein that promotes apoptosis by binding to and

antagonizing IAP proteins. Cell. 2000;102(1):43-53.

3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z,

Alnemri ES. Molecular Determinants of the Caspase-promoting

Activity of Smac/DIABLO and Its Role in the Death Receptor

Pathway. J Biol Chem. 2000;275(46):36152-36157.

4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and

biochemical basis of apoptotic activation by Smac/DIABLO. Nature.

2000;406(6798):855-62.

5. Liu Z, Sun C, Olejniczak ET, Meadows RP, Betz SF, Oost T,

Herrmann J, Wu JC, Fesik SW. Structural basis for binding of

Smac/DIABLO to the XIAP BIR3 domain. Nature.

2000;408(6815):1004-8.

6. Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y. Structural

basis of IAP recognition by Smac/DIABLO. Nature.

2000;408(6815):1008-12.

(WD0102)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-mSmac / DIABLO (CT)

CATALOG No.: 2411

BACKGROUND:

The inhibitor of apoptosis proteins (IAPs) regulate

programmed cell death by inhibiting members of the caspase

family of enzymes. A novel mammalian protein that binds to

IAPs and neutralizes the inhibitory effect of IAPs on

caspases was recently identified and designated

Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial

protein that is released along with cytochrome c during

apoptosis and activates cytochrome c/Apaf-1/capase-9

pathway. Analysis of the structural basis of Smac/DIABLO

reveals that the N-terminal amino acids are required for

binding of Smac/DIABLO to IAPs and activation of caspases

(3-6). Smac/DIABLO is expressed in a variety of human and

mouse tissues (1,2).

SOURCE:

Rabbit anti-mSmac/DIABLO (CT) polyclonal antibody was

raised against a peptide (SDEGADQEEEAYLRED)

corresponding to amino acids 222 to 237 of murine

Smac/DIABLO (2).

APPLICATION:


Smac/DIABLO by Western blot at 0.5 to 1 µg/ml. mouse

heart tissue lysate can be used as positive control and a 25

kDa band can be detected. It is human, mouse, and rat

reactive. For research use only. STORAGE:




RELATED PRODUCTS:


competition studies (Catalog No. 2411P). Mouse heart

tissue lysate, 200 µg at 2 mg/ml, is available for positive


Western blot analysis of Smac in

mouse heart tissue lysate in the


blocking peptide (2411P) and in

rat heart tissue lysate with anti-

mSmac at 1 µg /ml.

REFERENCES: 1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein

that promotes cytochrome c-dependent caspase activation by

eliminating IAP inhibition. Cell. 2000;102(1):33-42.

2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid

GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a

mammalian protein that promotes apoptosis by binding to and

antagonizing IAP proteins. Cell. 2000;102(1):43-53.

3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang

Z, Alnemri ES. Molecular Determinants of the Caspase-promoting

Activity of Smac/DIABLO and Its Role in the Death Receptor

Pathway. J Biol Chem. 2000;275(46):36152-36157.

4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and

biochemical basis of apoptotic activation by Smac/DIABLO.

Nature. 2000;406(6798):855-62.

5. Liu Z, Sun C, Olejniczak ET, Meadows RP, Betz SF, Oost T,

Herrmann J, Wu JC, Fesik SW. Structural basis for binding of

Smac/DIABLO to the XIAP BIR3 domain. Nature.

2000;408(6815):1004-8.

6. Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y.

Structural basis of IAP recognition by Smac/DIABLO. Nature.

2000;408(6815):1008-12.

(WD0102)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-APRIL (ED2) TALL-2, TRDL-1

CATALOG No.: 2415

BACKGROUND:




designated APRIL (for a proliferation-inducing ligand), TALL-

2 (for TNF- and ApoL-related Leukocyte-expressed Ligand

2), and TRDL-1α (for TNF related death ligand 1α) in human

and mouse (1-4). Two receptors for APRIL were recently

identified and designated TACI and BCMA (5-7). APRIL

stimulates B and T cell proliferation, triggers humoral

immune responses, activates NF-kB, and induces cell death

(1-7). APRIL and its close relative of BlyS and their

receptors BCMA and TACI are involved in diseases of

autoimmunity and cancer (8,9).

SOURCE:

Rabbit anti-APRIL (ED2) polyclonal antibody was raised

against a synthetic peptide (CIRSMPSHPDRAYNS)

corresponding to amino acids 196 to 210 of human APRIL

(1-3).

APPLICATION:

This antibody can be used for detection of APRIL by

Western blot at 2-4 µg/ml. HL60 or Jurkat cell lysate can be



STORAGE:




RELATED PRODUCT:



HL60 cell lysate, 200 µg at 2 mg/ml, is available for positive


Western blot analysis of APRIL

expression in Jurkat cell lysates

in the absence (A) or presence

(B) of blocking peptide with anti-

APRIL at 2 µg /ml.

REFERENCES: 1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor

necrosis factor family, stimulates tumor cell growth. J Exp Med.

1998;188(6):1185-90.

2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF

family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.

3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis

factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.

4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA

and TACI: system for regulating humoral immunity. Nat Immunol.

2000;1(3):252-6.

5. Wu Y, Bressette D, Carrell JA, et a. Tumor necrosis factor (TNF) receptor

superfamily member TACI is a high affinity receptor for TNF family members

APRIL and BLyS. J Biol Chem. 2000;275(45):35478-85.

6. Marsters SA, Yan M, Pitti RM, et al. Interaction of the TNF homologues

BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr

Biol. 2000;10(13):785-8.

7. Rennert P, Schneider P, Cachero TG, at al. A soluble form of B cell

maturation antigen, a receptor for the tumor necrosis factor family member

APRIL, inhibits tumor cell growth. J Exp Med. 2000;192(11):1677-84.

8. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are receptors for a

TNF homologue implicated in B-cell autoimmune disease. Nature

2000;404:995-9

9. Ware CF. APRIL and BAFF connect autoimmunity and cancer. J Exp

Med. 2000;192(11):F35-8.

(WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-PID / MTA2

CATALOG No.: 2443

BACKGROUND:



molecules involved in p53 pathway, including Chk2 (1),

p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2

(6), were recently discovered. The transcriptional activity of

p53 is modulated by protein stability and acetylation.

PID/MTA2, also termed MTA1-L1, was found to be a subunit

of nucleosome remodeling and deacetylating (NRD/NuRD)

complex (6-8). PID/MTA2 modulates the enzymatic activity

of the histone deacetylase complex and its expression

reduces the levels of acetylated p53. Deacetylation of p53 by

PID/MTA2 represses p53-dependent transcriptional

activation and modulates p53-mediated cell growth arrest

and apoptosis (6). PID/MTA2 is ubiquitously expressed in

human tissues (8).

SOURCE:

Rabbit anti-PID/MTA2 polyclonal antibody was raised

against a synthetic peptide (PAPSHPASTNEPIVLED)


PID/MTA2 (6), which differ from the mouse sequence by one

amino acid (7).

APPLICATION:

This antibody can be used for detection of PID/MTA2 by

Western blot at 0.5 to 1 µg/ml. HeLa cell lysate can be used

as positive control and a 75 kDa band can be detected. It is

human, mouse, and rat reactive and has no cross response

to MTA1. For research use only.

STORAGE:



year.

RELATED PRODUCTS:


competition studies (Catalog No. 2443P). HeLa cell lysate,

200 µg at 2 mg/ml, is available for positive control (Catalog

No. 1201).


PID/MTA2 expression in HeLa

whole cell lysates in the absence

(A) or presence (B) of blocking

peptide with anti-PID at 1 µg /ml.

REFERENCES: 1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle


2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S,

Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene

involved in a p53-dependent cell-cycle checkpoint for DNA

damage. Nature. 2000;404:42-9.

3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T,

Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the

Bcl-2 family and candidate mediator of p53-induced apoptosis.

Science. 2000;288(5468):1053-8.

4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T,

Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a

potential mediator of p53-dependent apoptosis, and its regulation

by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.

5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-

containing protein, is induced by p53 and promotes apoptosis. Nat

Genet. 2000;26:122-7.

6. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53

modulates its effect on cell growth and apoptosis. Nature.

2000;408:377-81.

7. Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A,

Reinberg D. Analysis of the NuRD subunits reveals a histone

deacetylase core complex and a connection with DNA methylation.

Genes Dev. 1999;13(15):1924-35.

8. Futamura M, Nishimori H, Shiratsuchi T, Saji S, Nakamura Y,

Tokino T.Molecular cloning, mapping, and characterization of a

novel human gene, MTA1-L1, showing homology to a metastasis-

associated gene, MTA1. J Hum Genet. 1999;44(1):52-6. (WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-MTBP (IN2)

CATALOG No.: 2447

BACKGROUND:



molecules involved in p53 network, including Chk2 (1),

p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6)

and MTBP (7), were recently discovered. The transcriptional

activity of p53 is modulated by posttranslational regulations

of the p53 protein including stabilization and acetylation. P53

transcriptionally activates MDM2 gene then the translated

MDM2 protein binds to p53 and promotes the degradation of

p53 leading to lowering the concentration of p53 protein.

MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A

recently discovered protein termed MTBP was found to bind

to MDM2 and to inhibit the modulation effect of MDM2 on

p53 (7). MTBP is expressed in a variety of normal tissues

(7).

SOURCE:

Rabbit anti-MTBP polyclonal antibody was raised against a

synthetic peptide (GAVECFEEEDSNSRESLS)

corresponding to amino acids 122 to 139 of human MTBP,

which differ from the mouse sequence by three amino acids

(7).

APPLICATION:

This antibody can be used for detection of MTBP by Western

blot at 0.5 to 1 µg/ml. K562 cell lysate can be used as

positive control and a 104 kDa band can be detected. For

research use only.

STORAGE:



for one year.

RELATED PRODUCTS:






MTBP expression in K562

whole cell lysates with anti-

MTBP at 1 µg /ml.

REFERENCES:

1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle


2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S,

Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene

involved in a p53-dependent cell-cycle checkpoint for DNA

damage. Nature. 2000;404:42-9.

3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T,

Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the

Bcl-2 family and candidate mediator of p53-induced apoptosis.

Science. 2000;288(5468):1053-8.

4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T,

Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a

potential mediator of p53-dependent apoptosis, and its regulation

by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.

5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-

containing protein, is induced by p53 and promotes apoptosis. Nat

Genet. 2000;26:122-7.

6. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53

modulates its effect on cell growth and apoptosis. Nature.

2000;408:377-81.

7 Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein

(MTBP) binds to MDM2 and induces a G1 arrest that is

suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.

(WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-PERP

CATALOG No.: 2451

BACKGROUND:



molecules involved in p53 network, including Chk2 (1),

p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6),

MTBP (7) and PERP (8), were identified and their genes

were cloned recently. PERP, also termed PIGPC1 and THW,

is a plasma membrane protein (8-10). p53 binds to the

promoter of PERP and transcriptionally activates PERP gene

then the translated PERP protein mediates the p53 induced

apoptosis (8). The expression of PERP causes cell death.

PERP is a mediator of p53 induced apoptosis. PERP has

sequence similarity to PMP-22/gas3 and is a new member of

the PMP-22/gas3 family (8).

SOURCE:

Rabbit anti-PERP polyclonal antibody was raised against a

synthetic peptide (EDDLLGNAKPRYFYTSA) corresponding

to amino acids 175 to 193 of human PERP, which differ from

the mouse sequence by three amino acids (7-9).

APPLICATION:

This antibody can be used for detection of PERP by Western

blot at 0.5 to 1 µg/ml. A431 cell lysate can be used as

positive control and a 21 kDa band can be detected. For

research use only.

STORAGE:



year.

RELATED PRODUCTS:






PERP expression in A431

whole cell lysates in the

absence (A) and presence (B)

of blocking peptide with anti-

PERP at 1 µg /ml.

REFERENCES: 1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7. 2. Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9. 3. Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8. 4. Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62. 5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis. Nat Genet. 2000;26:122-7. 6. Luo J, Su F, Chen D, et al. Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature. 2000;408:377-81. 7 Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90. 8. Attardi,L.D., Reczek,E.E., Cosmas,C., et al. PERP, an apoptosis-associated target of p53, is a novel member of the PMP-22/gas3 family. Genes Dev. 2000;14 (6):704-718 9. Goltsov,A.A., Ren,C., Wang,J., et al. p53 regulates the expression of a novel four transmembrane protein-PERP/PIGPC1 in mouse and human prostate cancer. GeneBank database 10. Hildebrandt,T., Weidle,U., Preiherr,J. et al. Identification of THW, a putative new tumor suppressor gene. GeneBank database (WD0105)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-21 (IN)

CATALOG No.: 2465

BACKGROUND:

A novel cytokine was recently identified in human and mouse

and designated IL-21 (1), which has significant homology to

IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also

termed NILR for novel Interleukin receptor) is a new member

of the class I cytokine receptor family (1,2). IL-21R forms a

complex with the common cytokine receptor γ chain, γc, and

mediates IL-21 signaling (3,4). IL-21 and its receptor activate

JAK-STAT signaling pathway. IL-21 is expressed in activated

T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in

the proliferation and maturation of NK, B and T cell

populations.

SOURCE:

Rabbit anti-IL-21 polyclonal antibody was raised against a

synthetic peptide (TCPSCDSYEKKPPKE) corresponding to

amino acids 121 to 135 of human IL-21 precursor (1).

APPLICATION:

This antibody can be used for detection of IL-21 by Western

blot at 0.5 to 1 µg/ml. Human HL-60 and THP-1 cell lysates

can be used as positive control and an approximately 18 kDa

band can be detected.


STORAGE:



for one year.

RELATED PRODUCTS:



Human HL-60 cell lysate, 200 µg at 2 mg/ml, is available for


Western blot analysis of IL-21

expression in HL-60 cell lysate in

the absence (lane A) or presence

of blocking peptide (lane B) with

anti-IL-21 (IN) at 1 µg /ml.

REFERENCES:

1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and

its receptor are involved in NK cell expansion and regulation of

lymphocyte function. Nature. 2000;408(6808):57-63.

2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I

cytokine receptor most related to the IL-2 receptor beta chain. Proc

Natl Acad Sci USA. 2000;97(21):11439-44.

3. Asao H, Okuyama C, Kumaki S, et al. the common gamma-

chain is an indispensable subunit of the IL-21 receptor complex. J

Immunol. 2001;167(1):1-5.

4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the

gamma(c)-dependent network? Curr Biol. 2001;11(5):R175-7.

(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-21R (ED)

CATALOG No.: 2469

BACKGROUND:

A novel cytokine related to IL-2 and IL-15 was recently

identified and designated IL-21 (1). The receptor for IL-21

(IL-21R, also termed NILR for novel Interleukin receptor) is a

new member of the class I cytokine receptor family (1,2). IL-

21R forms a complex with the common cytokine receptor γ

chain, γc, and mediates IL-21 signaling (3,4). Both IL-21R

and the γc are necessary for the IL-21 function. IL-21 and its

receptor activate JAK-STAT signaling pathway. IL-21R is

expressed in spleen, thymus, natural killer (NK), T and B cell

lines. IL-21 plays a role in the proliferation and maturation of

NK, B and T cell populations.

SOURCE:

Rabbit anti-IL-21R polyclonal antibody was raised against a

synthetic peptide (NITDQSGNYSQECGS) corresponding to

amino acids 97 to 111 of human IL-21R precursor (1).

APPLICATION:

This antibody can be used for detection of IL-21R by

Western blot at 0.5 to 1 µg/ml. Human Raji and A431 cell

lysates can be used as positive control and an approximately

60 kDa band can be detected.


STORAGE:



for one year.

RELATED PRODUCTS:



Human Raji cell lysate, 200 µg at 2 mg/ml, is available for


Western blot analysis of IL-21R

expression in human Raji cell

lysate with anti-IL-21R (ED) at 1

µg /ml.

REFERENCES:






Natl Acad Sci USA. 2000;97(21):11439-44.



Immunol. 2001;167(1):1-5.



(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-21R (NT)

CATALOG No.: 2471

BACKGROUND:

A novel cytokine related to IL2 and IL15 was recently

identified and designated IL-21 (1). The receptor for IL-21

(IL-21R, also termed NILR for novel Interleukin receptor) is a

new member of the class I cytokine receptor family (1,2). IL-

21R forms a complex with the common cytokine receptor γ

chain, γc, and mediates IL-21 signaling (3,4). Both IL-21R

and the γc are necessary for the IL-21 function. IL-21 and its

receptor activate JAK-STAT signaling pathway. IL-21R is

expressed in spleen, thymus, natural killer (NK), T and B cell

lines. IL-21 plays a role in the proliferation and maturation of

NK, B and T cell populations.

SOURCE:


synthetic peptide (CPDLVCYTDYLQT) corresponding to


APPLICATION:


Western blot at 0.5 to 1 µg/ml. Human Raji and A431 cell

lysates can be used as positive control and an approximately

60 kDa band can be detected.


STORAGE:



for one year.

RELATED PRODUCTS:



Human Raji cell lysate, 200 µg at 2 mg/ml, is available for



21R expression in human Raji

cell lysate with anti-IL-21R

(NT) at 1 µg /ml.

REFERENCES:






Natl Acad Sci USA. 2000;97(21):11439-44.



Immunol. 2001;167(1):1-5.



(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-TCCR (NT)

CATALOG No.: 2481

BACKGROUND:

Upon antigen challenge, T-helper cells differentiate into two

functional distinct subsets, Th1 and Th2. Th1 cells produce

IL-2, IFN-γ and lymphotoxin-β that augment cell mediated

immune response while Th2 cells secrete IL-4, IL-5, and IL-

10 that enhance humoral immunity. The function of T-helper

cells is regulated by cytokines. A novel cytokine receptor

was recently identified and cloned (1,2). It is a new member

in the type I cytokine receptor family and designated TCCR

for T-cell cytokine receptor and WSX-1 (1,2). TCCR

deficient mice had impaired Th1 responses to protein

antigen challenge, including decreased levels of IFN-γ and

Th1-dependent antibody IgG2a (1). TCCR is predominately

expressed in thymus, spleen, lymph notes and peripheral

blood leukocytes.

SOURCE:

Rabbit anti-TCCR polyclonal antibody was raised against a

synthetic peptide (GSAGPLQCYGVGPLGD)

corresponding to amino acids 44 to 59 of human TCCR

precursor (1),

APPLICATION:

This antibody can be used for detection of TCCR by Western

blot at 0.5 to 1 µg/ml. Human spleen tissue lysate can be


can be detected.


STORAGE:





TCCR expression in human

spleen tissue lysates with

anti-TCCR at 1 µg /ml.

RELATED PRODUCTS:





REFERENCES:

1. Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A,

Grewal IS and de Sauvage FJ. Development of Th1-type immune

responses requires the type I cytokine receptor TCCR Nature

2000;407(6806):916-920

2. Sprecher,C.A., Grant,F.J., Baumgartner,J.W., Presnell,S.R.,

Schrader,S.K., Yamagiwa,T., Whitmore,T.E., O'Hara,P.J. and

Foster,D.F. Cloning and characterization of a novel class I cytokine

receptor Biochem. Biophys. Res. Commun. 1998;246(1):82-90

(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-TCCR (CT)

CATALOG No.: 2483

BACKGROUND:

Upon antigen challenge, T-helper cells differentiate into two

functional distinct subsets, Th1 and Th2. Th1 cells produce

IL-2, IFN-γ and lymphotoxin-β that augment cell mediated

immune response while Th2 cells secrete IL-4, IL-5, and IL-

10 that enhance humoral immunity. The function of T-helper

cells is regulated by cytokines. A novel cytokine receptor

was recently identified and cloned (1,2). It is a new member

in the type I cytokine receptor family and designated TCCR

for T-cell cytokine receptor and WSX-1 (1,2). TCCR

deficient mice had impaired Th1 responses to protein

antigen challenge, including decreased levels of IFN-γ and

Th1-dependent antibody IgG2a (1). TCCR is predominately

expressed in thymus, spleen, lymph notes and peripheral

blood leukocytes.

SOURCE:

Rabbit anti-TCCR polyclonal antibody was raised against a

synthetic peptide (SGYEKHFLPTPEELG) corresponding to

amino acids 611 to 625 of human TCCR precursor (1). This

sequence is identical to that of mouse TCCR (1,2)

APPLICATION:

This antibody can be used for detection of TCCR by Western

blot at 0.5 to 1 µg/ml. Human spleen tissue lysate can be


can be detected. It is human, mouse, and rat reactive.


STORAGE:





TCCR expression in human

spleen tissue lysate with anti-

TCCR-CT at 1 µg /ml.

RELATED PRODUCTS:



Human spleen tissue lysate, 200 µg at 2 mg/ml, is available


REFERENCES:

1. Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A,

Grewal IS and de Sauvage FJ. Development of Th1-type immune

responses requires the type I cytokine receptor TCCR Nature

2000;407(6806):916-920

2. Sprecher,C.A., Grant,F.J., Baumgartner,J.W., Presnell,S.R.,

Schrader,S.K., Yamagiwa,T., Whitmore,T.E., O'Hara,P.J. and

Foster,D.F. Cloning and characterization of a novel class I cytokine

receptor Biochem. Biophys. Res. Commun. 1998;246(1):82-90

(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-22R (CT)

CATALOG No.: 2489

BACKGROUND:

A novel cytokine, designated IL-TIF for IL-10 related T cell-

derived inducible factor and IL-22, was recently identified (1-

3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and

IL-TIF-R1 chain) is a new member of the class II cytokine

receptor family (3,4). IL-22R forms a complex with IL-10

receptor β chain and mediates IL-22 signaling. IL-22 and its

receptor activate JAK-STAT signaling pathway. IL22R is

expressed in normal liver and kidney and their cell lines

HepG2 and TK-10. A soluble form of IL-22 receptor, also

termed IL-22 binding protein (IL-22BP) and IL-22 receptor-α

2 (IL-22RA2), was identified very recently (5-7). IL-22BP

prevents binding of IL-22 to the functional cell surface IL-22R

complex and neutralizes IL-22 activity. LPS induces IL-22

expression, which indicates the role of IL-22 in inflammatory

response.

SOURCE:


synthetic peptide (DSLFRGLALTVQWES) corresponding to


APPLICATION:


Western blot at 0.5 to 1 µg/ml. Human HepG2 cell lysate can




STORAGE:



for one year.

RELATED PRODUCTS:


competition studies (Catalog No. 2489P). Human HepG2

cell lysate, 200 µg at 2 mg/ml, is available for positive



22R expression in human

HepG2 cell lysate with anti-

IL-22R (CT) at 1 µg /ml.

REFERENCES: 1. Dumoutier L, Louahed J, Renauld JC. Cloning and

characterization of IL-10-related T cell-derived inducible factor (IL-

TIF), a novel cytokine structurally related to IL-10 and inducible by

IL-9. J Immunol. 2000;164(4):1814-9.

2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human

interleukin-10-related T cell-derived inducible factor: molecular

cloning and functional characterization as an hepatocyte-

stimulating factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.

3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel

human cytokine that signals through the interferon receptor-related

proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.

4. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al.

Identification of the functional interleukin-22 (IL-22) receptor

complex: J Biol Chem. 2001;276(4):2725-32.


Identification, cloning, and characterization of a novel soluble

receptor that binds IL-22 and neutralizes its activity. J Immunol.

2001;166(12):7096-103.

6. Dumoutier L, Lejeune D, Colau D, Renauld JC. Cloning and

characterization of il-22 binding protein, a natural antagonist of il-

10-related t cell-derived inducible factor/il-22. J Immunol.

2001;166(12):7090-5.

7. Xu W, Presnell SR, Parrish-Novak J, et al. A soluble class II

cytokine receptor, IL-22RA2, is a naturally occurring IL-22

antagonist. Proc Natl Acad Sci USA. 2001 in press (WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-IL-22R (NT)

CATALOG No.: 2497

BACKGROUND:

A novel cytokine, designated IL-TIF for IL-10 related T cell-

derived inducible factor and IL-22, was recently identified (1-

3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and

IL-TIF-R1 chain) is a new member of the class II cytokine

receptor family (3,4). IL-22R forms a complex with IL-10

receptor β chain and mediates IL-22 signaling. IL-22 and its

receptor activate JAK-STAT signaling pathway. IL22R is

expressed in normal liver and kidney and their cell lines

HepG2 and TK-10. A soluble form of IL-22 receptor, also

termed IL-22 binding protein (IL-22BP) and IL-22 receptor-α

2 (IL-22RA2), was identified very recently (5-7). IL-22BP

prevents binding of IL-22 to the functional cell surface IL-22R

complex and neutralizes IL-22 activity. LPS induces IL-22

expression, which indicates the role of IL-22 in inflammatory

response.

SOURCE:


synthetic peptide (QSSNFENILTWDSGPE) corresponding to


APPLICATION:


Western blot at 0.5 to 1 µg/ml. Human HepG2 cell lysate can




STORAGE:



for one year.

RELATED PRODUCTS:


competition studies (Catalog No. 2497P). Human HepG2

cell lysate, 200 µg at 2 mg/ml, is available for positive


Western blot analysis of IL-22R

expression in human HepG2 cell

lysate with anti-IL-22R (NT) at 1

µg /ml.

REFERENCES: 1. Dumoutier L, Louahed J, Renauld JC. Cloning and

characterization of IL-10-related T cell-derived inducible factor (IL-

TIF), a novel cytokine structurally related to IL-10 and inducible by

IL-9. J Immunol. 2000;164(4):1814-9.

2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human

interleukin-10-related T cell-derived inducible factor: molecular

cloning and functional characterization as an hepatocyte-

stimulating factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.

3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel

human cytokine that signals through the interferon receptor-related

proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.


Identification of the functional interleukin-22 (IL-22) receptor

complex: J Biol Chem. 2001;276(4):2725-32.


Identification, cloning, and characterization of a novel soluble

receptor that binds IL-22 and neutralizes its activity. J Immunol.

2001;166(12):7096-103.

6. Dumoutier L, Lejeune D, Colau D, Renauld JC. Cloning and

characterization of il-22 binding protein, a natural antagonist of il-

10-related t cell-derived inducible factor/il-22. J Immunol.

2001;166(12):7090-5.

7. Xu W, Presnell SR, Parrish-Novak J, et al. A soluble class II

cytokine receptor, IL-22RA2, is a naturally occurring IL-22

antagonist. Proc Natl Acad Sci USA. 2001 in press (WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Act1 / CIKS (CT)

CATALOG No.: 2499

BACKGROUND:





response to extracellular stimuli. NF-κB associates with IκB

proteins in the cell cytoplasm, which inhibit NF-κB activity.

IκB is phosphorylated by IκB kinase (IKK) complex that

contains IKKα, IKKβ, and IKKγ. A novel molecule that

associates with and activates IKK was recently identified and

designated CIKS (for connection to IKK and SAPK/JNK) and

Act1 (for NF-κB activator 1) (1,2). CIKS directly interacts with

IKKγ. CIKS/Act1 also activates activating transcription factor

(ATF) and activator protein 1 (AP-1) through Jun kinase

(JNK). These results indicate that CIKS/Act1 is involved in

the inflammation and stress responses. CIKS/Act1 is

ubiquitously expressed in human tissues.

SOURCE:

Rabbit anti-CIKS polyclonal antibody was raised against a

synthetic peptide (REEEYVAPPRGPLPT) corresponding to

amino acids 554 to 568 of human CIKS (1,2).

APPLICATION:

This antibody can be used for detection of CIKS by Western

blot at 0.5 to 1 µg/ml. Human placenta tissue lysate can be


can be detected.


STORAGE:




Western blot analysis of CIKS

expression in human placenta

tissue lysate with anti-CIKS (CT)

at 1 µg /ml.

RELATED PRODUCTS:



Human placenta tissue lysate, 200 µg at 2 mg/ml, is


REFERENCES:

1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U.

CIKS, a connection to Ikappa B kinase and stress-activated protein

kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.

2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D,

Haag M, Stark GR. Act1, an NF-kappa B activating protein. Proc

Natl Acad Sci USA. 2000;97(19):10489-93.

(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Act1 / CIKS (NT)

CATALOG No.: 2501

BACKGROUND:





response to extracellular stimuli. NF-κB associates with IκB

proteins in the cell cytoplasm, which inhibit NF-κB activity.

IκB is phosphorylated by IκB kinase (IKK) complex that

contains IKKα, IKKβ, and IKKγ. A novel molecule that

associates with and activates IKK was recently identified and

designated CIKS (for connection to IKK and SAPK/JNK) and

Act1 (for NF-κB activator 1) (1,2). CIKS directly interacts with

IKKγ. CIKS/Act1 also activates activating transcription factor

(ATF) and activator protein 1 (AP-1) through Jun kinase

(JNK). These results indicate that CIKS/Act1 is involved in

the inflammation and stress responses. CIKS/Act1 is

ubiquitously expressed in human tissues.

SOURCE:

Rabbit anti-CIKS polyclonal antibody was raised against a

synthetic peptide (PPQLQETRMNRSIP) corresponding to

amino acids 2 to 15 of human CIKS (1,2).

APPLICATION:

This antibody can be used for detection of CIKS by Western

blot at 0.5 to 1 µg/ml. Human placenta tissue lysate can be


can be detected.


STORAGE:





CIKS expression in human

lung (lane A) and placenta

(lane B) tissue lysates with

anti-CIKS (NT) at 1 µg /ml.

RELATED PRODUCTS:



Human placenta tissue lysate, 200 µg at 2 mg/ml, is


REFERENCES:

1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U.

CIKS, a connection to Ikappa B kinase and stress-activated protein

kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.

2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D,

Haag M, Stark GR. Act1, an NF-kappa B activating protein. Proc

Natl Acad Sci USA. 2000;97(19):10489-93.

(WD0108)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Livin, KIAP

CATALOG No.: 2505

BACKGROUND:






inhibits cell death proteases. A novel member in the IAP

protein family was recently identified and designated Livin

and KIAP for kidney IAP (1,2). Livin/XIAP contains a single

baculoviral IAP repeat (BIR) domain and a RING finger

domain and has two isoforms termed Livin-α and Livin-β

(1,3). Transfection of Livin in cells resulted in protection from

apoptosis induced by FADD, BAX, RIP, RIP3 and DR6 (1).

Livin has direct interaction with several caspases including

caspase-3, -7, and –9. Livin inhibits the activation of

caspase-9 induced by Apaf-1, cytochrome c, and dATP. The

two isoforms of Livin ? ? ? ? ? ? ?to ?????different function

and tissue distribution.

SOURCE:

Rabbit anti-Livin polyclonal antibody was raised with a

synthetic peptide (CRAPVRSRVRTFLS) corresponding to

amino acids 264 to 280 of the short form and 281 to 298 of

the long form of human Livin (1,3). This sequence is identical

between α and β ????s ?? the Livin ? ? ? ? ? ? ? ? .

APPLICATION:

This antibody can be used for detection of Livin by Western

blot at 2 to 4 µg/ml. Human Raji cell lysate can be used as a

positive control and a band at 33 kDa can be detected. A

lower but much weaker band at 30 kDa was detected in Raji

cell lysate, which may represent the short

???????????????


Western blot analysis of Livin

expression in human Raji cell lysate with

anti-Livin at 4 µg/ml.

STORAGE: It is supplied as immunoaffinity

chromatography purified IgG, 100 µg in 200 µl of PBS

containing 0.02% sodium azide. Store at 4°C, stable for

one year.

RELATED PRODUCTS:



Human Raji cell lysate (Catalog No. 1207), 200 µg at 2

mg/ml, is available for positive control.

REFERENCES:

1. Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis

protein family member. J Biol Chem. 2001;276(5):3238-46.

2. Lin JH, Deng G, Huang Q, Morser J. KIAP, a novel member of

the inhibitor of apoptosis protein family. Biochem Biophys Res

Commun. 2000;279(3):820-31.

3. Ashhab Y, Alian A, Polliack A, Panet A, Yehuda DB.

Two splicing variants of a new inhibitor of apoptosis gene with

different biological properties and tissue distribution pattern.

FEBS Lett. 2001;495(1-2):56-60.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-CARD9

CATALOG No.: 2515

BACKGROUND:

Apoptosis is related to many diseases and development.

Cell death signals are transduced by death domain (DD),

death effector domain (DED), and caspase recruitment

domain (CARD) containing molecules. CARD containing

proteins include some caspases, Apaf-1, CARD4, IAPs,

RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-

containing protein was recently identified and designated

CARD9, which interacts with the CARD activation domain of

BCL-10 (1). CARD9 associates with BCL-10 and forms a

complex within cells. CARD9 induces apoptosis and

activates NF-κB. CARD9 is an upstream activator of BCL-10

and NF-κB signaling.

SOURCE:

Rabbit anti-CARD9 polyclonal antibody was raised against a

synthetic peptide (DRENTTGSDNTDTEGS) corresponding to

amino acids 521 to 536 of human CARD9 (1). The

sequence is different from that of rat origin by two amino

acids (1).

APPLICATION:

This antibody can be used for detection of CARD9 by

Western blot at 0.5 to 1 µg/ml. Human PC-3 and MDA-MB-

361 cell lysates can be used as a positive control and a band

at approximately 59 kDa can be detected.


STORAGE:



for one year.

Western blot analysis of CARD9

expression in human MDA-MB-361

(A) and PC-3 (B) cell lysate with anti-

CARD9 at 2.5 µg /ml.

RELATED PRODUCTS:



Human PC-3 lysate, 200 µg at 2 mg/ml, is available for


REFERENCES:

1. Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL,

Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES.

CARD9 is a novel caspase recruitment domain-containing protein that

interacts with BCL10/CLAP and activates NF-kappa B. J Biol Chem.

2000;275(52):41082-6.

(RD0202)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ILP-2

CATALOG No.: 3017

BACKGROUND:





proteins form a conserved gene family including IAP,

XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits

specific proteases. A novel member in the IAP protein family

was recently identified and designated ILP-2 for IAP-like

protein-2 (1). ILP-2 has high homology to ILP-1, but is

encoded by a distinct gene that is solely expressed in testis

of tested normal human tissues (1). ILP-2, unlike ILP-1, has

no inhibitory effect on Fas and TNF induced apoptosis, but

potently inhibits apoptosis induced by overexpression of Bax

or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts

with the processed caspase-9. These results suggest that

ILP-2 is a novel IAP family member with restricted specificity

for caspase-9.

SOURCE:

Rabbit anti-ILP-2 polyclonal antibody was raised with a

synthetic peptide (TGYEARLITFGT) corresponding to amino

acids 2 to 13 of human ILP-2 (1).

APPLICATION:

This antibody can be used for detection of ILP-2 by Western

blot at 1 to 2 µg/ml. Human HepG2 or MOLT4 cell lysate

can be used as a positive control and a band at

approximately 33 kDa can be detected. It is human, mouse,

and rat reactive.


STORAGE:




Western blot analysis of ILP-2

expression in human HepG2 (lane 1)

and MOLT4 (lane 2) cell lysates and

mouse spleen (lane 3) tissue lysates

with anti-ILP-2 at 1 µg/ml.

RELATED PRODUCTS:



Human HepG2 cell lysate (Catalog No. 1211), 200 µg at 2


REFERENCES:

1. Richter BW, Mir SS, Eiben LJ, Lewis J, Reffey SB, Frattini A, Tian

L, Frank S, Youle RJ, Nelson DL, Notarangelo LD, Vezzoni P,

Fearnhead HO, Duckett CS. Molecular cloning of ILP-2, a novel

member of the inhibitor of apoptosis protein family. Mol Cell Biol.

2001;21(13):4292-301.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-ARTS

CATALOG No.: 3025

BACKGROUND:

Apoptosis is related to many diseases and development.

Mitochondrial proteins, such as cytochrome c, Apaf-1, and

AIF play important role in apoptosis. A novel mitochondrial

septin-like protein was identified recently and designated

ARTS for apoptosis related protein in TGF-β signaling

pathway (1). ARTS that is encoded by the human septin

H5/PNUTL2/CDCrel2b gene (1-4) is located to mitochondria

and translocates to the nucleus when apoptosis occurs.

ARTS is expressed in many tissues. It enhances cell death

induced by TGF-β and, to a lesser extent, by other apoptotic

agents, such as TNF-α and Fas ligand.

SOURCE:

Rabbit anti-ARTS polyclonal antibody was raised against a

synthetic peptide (KRFLEDTTDDGE) corresponding to

amino acids 3 to 14 of human ARTS (1).

APPLICATION:

This antibody can be used for detection of ARTS by Western

blot at 0.5 to 2 µg/ml. Human lung, kidney and spleen tissue

lysates can be used as a positive control and a band at 32

kDa can be detected.


STORAGE:




RELATED PRODUCTS:



Human lung tissue lysate, 200 µg at 2 mg/ml, is available for


Western blot analysis of ARTS

expression in human lung (lane 1),

spleen (lane 2), and kidney (lane 3)

tissue lysates with anti-ARTS at 2 µg /ml.

REFERENCES:

1. Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial

septin-like protein, ARTS, mediates apoptosis dependent on its P-

loop motif. Nat Cell Biol 2000;2(12):915-21

2. Depraetere V. The ARTS of apoptosis. Nat Cell Biol.

2000;2(12):E219.

3. Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L.

Characterization of a novel gene, PNUTL2, on human chromosome

17q22-q23 and its exclusion as the Meckel syndrome gene.

Genomics 1999;55 (1):122-125

4. Zieger,B., Tran,H., Hainmann,I., Wunderle,D., Zgaga-Griesz,A.,

Blaser,S. and Ware,J. Characterization and expression analysis of

two human septin genes, PNUTL1 and PNUTL2. Gene

2000;261(2):197-203

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bmf (CT)

CATALOG No.: 3029

BACKGROUND: Apoptosis is related to many diseases and development.



homology 3 (BH3) domain is a potent death domain. BH3-

only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and

PUMA, form a growing subclass of the Bcl-2 family. A novel

BH3-only protein was recently identified in human and

mouse and designated Bmf (for Bcl-2-modifing factor) (1).

The BH3 domain in Bmf is required both for binding to Bcl-2

proteins and for triggering apoptosis. In healthy cells, Bmf

associates with the dynein light chain 2 (DLC2) component

of the myosin V motors and is sequestered by the cell’s actin

cytoskeleton. Disruption of the actin cytoskeleton, either by

depolymerization of actin filaments or by detachment of cells

from the extracellular matrix, triggers release and activation

of Bmf, initiating the downstream apoptotic program (1,2).

Bmf is constitutively expressed in many tissues (1,2).

SOURCE:

Rabbit anti-Bmf polyclonal antibody was raised with a

synthetic peptide (ALNGEENRNGAGPR) corresponding to

amino acids 171 to 184 of human Bmf (1).

APPLICATION:

This antibody can be used for detection of Bmf by Western

blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a

positive control and a band at approximately 25 kDa can be

detected.


STORAGE:




RELATED PRODUCTS:



HepG2 (Catalog No. 1204) cell lysate, 200 µg at 2 mg/ml, is

available for positive control

REFERENCES:

1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L,

Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only

protein regulated by interaction with the myosin V actin motor

complex, activated by anoikis.

Science. 2001;293(5536):1829-32.

2. Hunt A, Evan G. Apoptosis. Till death us do part. Science.

2001;293(5536):1784-5.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-Bmf (NT)

CATALOG No.: 3031

BACKGROUND: Apoptosis is related to many diseases and development.



homology 3 (BH3) domain is a potent death domain. BH3-

only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and

PUMA, form a growing subclass of the Bcl-2 family. A novel

BH3-only protein was recently identified in human and

mouse and designated Bmf (for Bcl-2-modifing factor) (1).

The BH3 domain in Bmf is required both for binding to Bcl-2

proteins and for triggering apoptosis. In healthy cells, Bmf

associates with the dynein light chain 2 (DLC2) component

of the myosin V motors and is sequestered by the cell’s actin

cytoskeleton. Disruption of the actin cytoskeleton, either by

depolymerization of actin filaments or by detachment of cells

from the extracellular matrix, triggers release and activation

of Bmf, initiating the downstream apoptotic program (1,2).

Bmf is constitutively expressed in many tissues (1,2).

SOURCE:

Rabbit anti-Bmf polyclonal antibody was raised with a

synthetic peptide (EPSQCVEELEDDV) corresponding to

amino acids 2 to 14 of human Bmf (1). This sequence differs

from that of mouse by one amino acid.

APPLICATION:

This antibody can be used for detection of Bmf by Western

blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a

positive control and a band at approximately 25 kDa can be

detected. It is human and mouse reactive.


STORAGE:




Western blot analysis of Bmf

expression in human HepG2 (lane 1)

and 293 (lane 2) cell lysates with anti-

Bmf at 2 µg /ml.

RELATED PRODUCTS:



HepG2 (Catalog No. 1204) cell lysate, 200 µg at 2 mg/ml, is

available for positive control

REFERENCES:

1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L,

Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only

protein regulated by interaction with the myosin V actin motor

complex, activated by anoikis.

Science. 2001;293(5536):1829-32.

2. Hunt A, Evan G. Apoptosis. Till death us do part. Science.

2001;293(5536):1784-5.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-EndoG

CATALOG No.: 3035

BACKGROUND:

The fragmentation of nuclear DNA is a hallmark of apoptotic

cell death. The activities of caspase and nuclease are

involved in the DNA fragmentation. Caspase-activated

deoxyribonuclease (CAD), also termed DNA fragmentation

factor (DFF40), is one such nuclease, and is capable of

inducing DNA fragmentation and chromatin condensation

after cleavage by caspase-3 of its inhibitor ICAD/DFF45.

Caspase and CAD independent DNA fragmentation also

exists. Recent studies demonstrated that another nuclease,

endonuclease G (endoG), is specifically activated by

apoptotic stimuli and is able to induce nucleosomal

fragmentation of DNA independently of caspase and

DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease

that translocates to the nucleus and cleaves chromatin DNA

during apoptosis. The homologue of mammalian EndoG is

the first mitochondrial protein identified to be involved in

apoptosis in C. elegans (2). EndooG also cleaves DNA in

vitro (4).

SOURCE:

Rabbit anti-EndoG polyclonal antibody was raised with a

synthetic peptide (GGPRGPGELAKYGLP) corresponding to

amino acids 55 to 70 of human EndoG (5).

APPLICATION:

This polyclonal antibody can be used for detection of EndoG

by Western blot at 1 to 2 µg/ml. HepG2 and 3T3 cell lysates

can be used as positive control and a 35 kDa band can be

detected. It is human, mouse and rat reactive.


STORAGE:




Western blot analysis of EndoG in

mouse (M) 3T3 and human (H) HepG2

cell lysates with anti-EndoG at 2 µg/ml.

RELATED PRODUCTS:



HepG2 cell lysate (Catalog No. 1211), 200 µg at 2 mg/ml, is

available for positive control.

REFERENCES:

1. Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase

when released from mitochondria. Nature. 2001;412(6842):95-9.

2. Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial

endonuclease G is important for apoptosis in C. elegans. Nature.

2001;412(6842):90-4

3. Hengartner MO. Apoptosis. DNA destroyers. Nature.

2001;412(6842):27, 29.

4. Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant

human apoptotic endonuclease G on naked DNA and chromatin

substrates: cooperation with exonuclease and DNase I. J Biol

Chem. 2001;276(51):48404-9.

5. Tiranti V, Rossi E, Ruiz-Carrillo A, Rossi G, Rocchi M, DiDonato

S, Zuffardi O, Zeviani M. Chromosomal localization of mitochondrial

transcription factor A (TCF6), single-stranded DNA-binding protein

(SSBP), and endonuclease G (ENDOG), three human

housekeeping genes involved in mitochondrial biogenesis.

Genomics. 1995;25(2):559-64. (WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-PUMA (CT) bbc3

CATALOG No.: 3041

BACKGROUND:

Apoptosis is related to many diseases and development. The

p53 tumor-suppressor protein induces apoptosis through

transcriptional activation of several genes. A novel p53

inducible pro-apoptotic gene was identified recently and

designated PUMA (for p53 upregulated modulator of

apoptosis) and bbc3 (for Bcl-2 binding component 3) in

human and mouse (1-3). PUMA/bbc3 is one of the pro-

apoptotic Bcl-2 family members including Bax and Noxa,

which are also transcriptional targets of p53. The PUMA

gene encodes two BH3 domain-containing proteins termed

PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2,

localize to the mitochondria, and induce cytochrome c

release and apoptosis in response to p53. PUMA may be a

direct mediator of p53-induced apoptosis.

SOURCE:

Rabbit anti-PUMA polyclonal antibody was raised against a

synthetic peptide (PLPRGHRAPEMEPN) corresponding to

amino acids 180 to 193 of human PUMA-α (1). This

sequence is identical between α and β ? ? ? ? ? ?? ? ?the

? ? ? ? ?? ? ? ? ? ? ? ? .

APPLICATION:

This antibody can be used for detection of PUMA by

Western blot at 1 to 2 µg/ml. K562 or 3T3 cell lysate can be

used as a positive control and a band at approximately 23

kDa can be detected. A lower band at approximately 16 kDa

was detected in MOLT4 and U937 cells, which may

represent the PUMA-β ? ? ? ? ? It is human and mouse

reactive.


STORAGE:




Western blot analysis of PUMA

expression in human (H) K562 and

mouse (M) 3T3 cell lysates with anti-

PUMA at 2 µg /ml.

RELATED PRODUCTS:



K562 (Catalog No. 1204) or 3T3 (Catalog No. 1212) cell


REFERENCES:

1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is

induced by p53. Mol Cell. 2001;7(3):683-94.

2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA

induces the rapid apoptosis of colorectal cancer cells. Mol Cell.

2001;7(3):673-82.

3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ,

Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only

gene, is regulated by diverse cell death and survival signals. Proc

Natl Acad Sci U S A. 2001;98(20):11318-23.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-PUMA (NT) bbc3

CATALOG No.: 3043

BACKGROUND:




inducible pro-apoptotic gene was identified recently and

designated PUMA (for p53 upregulated modulator of

apoptosis) and bbc3 (for Bcl-2 binding component 3) in

human and mouse (1-3). PUMA/bbc3 is one of the pro-

apoptotic Bcl-2 family members including Bax and Noxa,

which are also transcriptional targets of p53. The PUMA

gene encodes two BH3 domain-containing proteins termed

PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2,

localize to the mitochondria, and induce cytochrome c

release and apoptosis in response to p53. PUMA may be a

direct mediator of p53-induced apoptosis.

SOURCE:

Rabbit anti-PUMA polyclonal antibody was raised with a

synthetic peptide (ARARQEGSSPEPVEG) corresponding to

amino acids 2 to 16 of human PUMA-α (1). This sequence is

identical between human and mouse PUMA.

APPLICATION:

This antibody can be used for detection of PUMA by

Western blot at 2 to 4 µg/ml. K562 cell lysate can be used

as a positive control and a band at approximately 23 kDa

can be detected.


STORAGE:




Western blot analysis of PUMA

expression in human K562 cell lysate

with anti-PUMA at 2 µg /ml.

RELATED PRODUCTS:



K562 cell lysate (Catalog No. 1204), 200 µg at 2 mg/ml, is

available for positive control.

REFERENCES:

1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is

induced by p53. Mol Cell. 2001;7(3):683-94.

2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA

induces the rapid apoptosis of colorectal cancer cells. Mol Cell.

2001;7(3):673-82.

3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ,

Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only

gene, is regulated by diverse cell death and survival signals. Proc

Natl Acad Sci U S A. 2001;98(20):11318-23.

(WD0204)


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Anti-p53DINP1, SIP

CATALOG No.: 3045

BACKGROUND:




inducible gene was identified recently and designated

p53DINP1 (for p53-dependent damage-inducible nuclear

protein 1) and SIP (for stress induced protein) in human and

mouse (1,2). A p53DINP1 antisense oligonucleotide inhibits

and overexpression of p53DINP1 enhances Ser46

phosphorylation of p53, induction of p53AIP1, and cell death

induced by DNA double-strand breaks (1). p53DINP1 may

regulate p53-dependent apoptosis through phosphorylation

at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene

encodes two proteins of 27 and 18 kDa in human and mouse

termed p53DINP1-α and p53DINP1-β or SIP27 and SIP18

(1,2). p53DINP1/SIP is expressed in many tissues and

induced by a variety of stress agents including UV stress,

mutagenic stress, heat shock, and oxidative stress (2).

SOURCE:

Rabbit anti-p53DINP1 polyclonal antibody was raised with a

synthetic peptide (MFQRLNKMFVGEVS) corresponding to

amino acids 1 to 14 of human p53DINP1 (1). This sequence

is identical between α and β ? ? ? ? ? ?? ? ?the

p53DINP1?????????, and differs by one amino acid from

those of mouse.

APPLICATION:

This antibody can be used for detection of p53DINP1 by

Western blot at 1 to 2 µg/ml. Human lung tissue lysate can

be used as a positive control and a band at 27 kDa can be

detected. A lower band at 18 kDa was detected in human

spleen, and mouse liver and kidney tissue lysates, which

may represent the p53DINP1-β ? ? ? ? ? It is human, mouse,

and rat reactive.


STORAGE:





p53DINP1 expression in human

lung tissue lysate with anti-

p53DINP1 at 2 µg/ml in the

absence (lane 1) or presence

(lane 2) of blocking peptide.

RELATED PRODUCTS:



Human lung tissue lysate (Catalog No. 1302), 200 µg at 2


REFERENCES:

1. Okamura S, Arakawa H, Tanaka T, Nakanishi H, Ng CC, Taya Y,

Monden M, Nakamura Y. p53DINP1, a p53-inducible gene,

regulates p53-dependent apoptosis. Mol Cell. 2001;8(1):85-94.

2. Tomasini R, Samir AA, Vaccaro MI, Pebusque MJ, Dagorn JC,

Iovanna JL, Dusetti NJ. Molecular and functional characterization of

the stress-induced protein (SIP) gene and its two transcripts

generated by alternative splicing. SIP induced by stress and

promotes cell death. J Biol Chem. 2001;276(47):44185-92.

(WD0204)

peptide Data Sheets


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IRAK (CT) peptide

CATALOG NO.: 1007P SEQUENCE: DRQGPEESDEFQS LOCATION: Amino acids 700 to 712 of human IRAK (1) APPLICATION:

The peptide is used for blocking the activity of anti-IRAK (CT, catalog number 1007). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-IRAK in Western blotting. For research use only. STORAGE:

It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science

1996;271:1128-31.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CXCR4 (NT) peptide

CATALOG NO.: 1009P SEQUENCE: MEGISIYTSDNYTE LOCATION: Amino acids 1 to 14 of human CXCR4 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-CXCR4 (NT, catalog number 1009). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-CXCR4 (NT) in Western blotting. For research use only. STORAGE:

It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Berson J.F. et al. J Virol 1996;70:6288-95. 2. Leoetscher M. et al. J Biol Chem 1994;269:232-237


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CXCR4 (EL) peptide

CATALOG NO.: 1012P SEQUENCE: DRYICDRFYPNDLWV LOCATION: Amino acids 182 to 196 of human CXCR4 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-CXCR4 (EL, catalog number 1012). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-CXCR4 (EL) in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE:

1. Berson J.F. et al. J Virol 1996;70:6288-95.

2. Leoetscher M. et al. J Biol Chem 1994;269:232-237


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CCR5 (NT) peptide

CATALOG NO.: 1112P SEQUENCE: SSPIYDINYYTSEPC LOCATION: Amino acids 6 to 20 of human CCR5 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-CCR5 (catalog number 1112). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-CCR5 in Western blotting.


1. Raport, CJ, et al. J Biol Chem 1996;271:17161-6

2. Combadiere, C. et al. J Leuk Biol 1996;60:147-52


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

TRAIL (CT) peptide

CATALOG NO.: 1113P SEQUENCE: CTNEHLIDMDHEASFFGA LOCATION: Amino acids 261 to 277 of human TRAIL (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-TRAIL (catalog number 1113). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-TRAIL (CT) in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch

C, Smith CA, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995;3:673-682

2. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by

Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996;271:12687-90


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

RAIDD (IN) peptide

CATALOG NO.: 1115P SEQUENCE: CAGDRLTGIPSHILNSSPSD LOCATION: Amino acids 99 to 117 of human RAIDD (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-RAIDD (catalog number 1115). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-RAIDD (IN) in Western blotting.


1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89 2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES.

CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP. Cancer Res 1997


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

RAIDD (CT) peptide

CATALOG NO.: 1117P SEQUENCE: CHNGLRAVEVDPSLLLHMLE LOCATION: Amino acids 181 to 199 of human RAIDD (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-RAIDD (catalog number 1117). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89 2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES.

CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP. Cancer Res 1997 57:615-619


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

DR3 (CT) peptide

CATALOG NO.: 1120P SEQUENCE: ERMGLDGCVEDLRSRLQRGP LOCATION: Amino acids 398 to 417 of human DR3 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-DR3 (catalog number 1120). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit

VM. Science, 1996;274:990-2. 2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN.

Nature, 1996;384:372-5.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Neurturin peptide

CATALOG NO.: 1121P SEQUENCE: DAHSRYHTVHELSARE LOCATION: Amino acids 178 to 193 of human neurturin (1) APPLICATION:

The peptide is used for blocking the activity of anti-neurturin (catalog number 1121). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Kotzbauer PT, Lampe PA, Heuckeroth RO, Golden JP, Creedon DJ, Johnson EM Jr, lbrandt J.

Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Eotaxin peptide

CATALOG NO.: 1123P

SEQUENCE: NH2-DSMKYLDQKSPTPKP-OH

LOCATION: Amino acids 83 to 97 of human eotaxin (1-2).

Application:

The peptide is used for blocking the antibody activity of eotaxin (catalog number 1123). It usually

blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of

antibody for 30 min at 37°C


STORAGE:

It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM

NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.

REFERENCE:

1. Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective

CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J

Biol Chem 1996;271:7725-30

2. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin.

Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment

of eosinophils. J Clin Invest 1996;97:604-12


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

SIRPα (CT) peptide

CATALOG NO.: 1125P SEQUENCE: KPEPSFSEYASVQVPRK LOCATION: Amino acids 487 to 503 of human SIRPα (1) APPLICATION:

The peptide is used for blocking the activity of anti-SIRPα (catalog number 1125). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Kharitonenkov A, et al. Nature 1997;386:181-186.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Caspase-10 (CT) peptide

CATALOG NO.: 1128P SEQUENCE: ISAQTPRPPMRRWSSVS LOCATION: Amino acids 505 to 521 of human caspase-10 (1) APPLICATION:

The peptide is used for blocking the activity of anti-caspase-10 (catalog number 1128). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2),

an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

NIK (CT) peptide

CATALOG NO.: 1129P SEQUENCE: SFAWSWRVKHGQLENRP LOCATION: Amino acids 931 to 947 of human NIK (1) APPLICATION:

The peptide is used for blocking the activity of anti-NIK (catalog number 1129). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3K-related kinase involved in NF-kappaB

induction by TNF, CD95 and IL-1. Nature 1997;385:540-544


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

TACE (CT) peptide

CATALOG NO.: 1131P SEQUENCE: ASFKLQRQNRVDSKETE LOCATION: Amino acids 807 to 823 of human TACE (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-TACE (CT, catalog number 1131). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-TACE in Western blotting. For research use only. STORAGE:

It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCES: 1. Black RA, Rauch CT, Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-

necrosis factor-alpha from cells. Nature 1997;385:729-733 2. Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor

tumour-necrosis factor-alpha. Nature 1997;385:733-736


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

GFRα-1 (IN) peptide CATALOG NO.: 1133P SEQUENCE: KNKPLGPAGSENEI LOCATION: Amino acids 369 to 382 of human GFRα-1 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-GFRα-1 (catalog number 1133). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z; Cupples R; Louis JC;

Hu S; Altrock BW; Fox GM. GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR-? , a novel receptor for GDNF. Cell, 1996;85:1113-24.

2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD; Gray C; Armanini MP;

Pollock RA; Herti F; Phillips HS; Goddard A; Moore MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF. Nature, 1996;382:80-83.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

GFRα-3 (IN) peptide CATALOG NO.: 1137P SEQUENCE: RFHRQLFSQDWADS LOCATION: Amino acids 347 to 360 of murine GFRα-3 (1) APPLICATION:

The peptide is used for blocking the activity of anti-GFRα-3 (catalog number 1137). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Naveilhan P, Baudet C, Mikaels A, Shen L, Westphal H, Ernfors P. Expression and regulation of

GFRalpha3, a glial cell line-derived neurotrophic factor family receptor. Proc Natl Acad Sci USA 1998;95:1295-1300.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

DR4 (CT) peptide CATALOG NO.: 1139P SEQUENCE: CDSGKFIYLEDGTGSAVSLE LOCATION: Amino acids 427 to 445 of human DR4 (1) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor

for the cytotoxic ligand TRAIL. Science; 1997;276:111-113


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

DFF45 (CT) peptide CATALOG NO.: 1141P SEQUENCE: KASPPGDLQNPKRARQDPT LOCATION: Amino acids 313 to 331 of human DFF45 (1) APPLICATION:

The peptide is used for blocking the activity of anti-DFF45 (catalog number 1141). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of

caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DFF45/35 (NT) peptide CATALOG NO.: 1148P SEQUENCE: EVTGDAGVPESGEIRTLKPC LOCATION: Amino acids 2 to 21 of human DFF45/35 (1) APPLICATION:

The peptide is used for blocking the activity of anti-DFF45/35 (catalog number 1148). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of

caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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MADD (NT) peptide CATALOG NO.: 1150P SEQUENCE: AAVHSSEEDLRTPPRPVSS LOCATION: Amino acids 1570 to 1588 of human MADD (1) APPLICATION:

The peptide is used for blocking the activity of anti-MADD (catalog number 1150). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel death domain protein that interacts with

the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J Biol Chem 1997;272:12069-12075


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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mFLIP (CT) peptide CATALOG NO.: 1156P SEQUENCE: DKVYAWNSGVSSKEKYS LOCATION: Amino acids 449 to 465 of human mFLIP (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-mFLIP (catalog number 1156). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,

Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195.

2. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM, Wallach D. CASH, a

novel caspase homologue with death effector domains. J Biol Chem 1997;272:19641-19644.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DR3 (ED) peptide CATALOG NO.: 1158P SEQUENCE: APCTEPCGNSTCLVCPQDT LOCATION: Amino acids 59 to 77 of human DR3 (1,2) APPLICATION:




Nature, 1996;384:372-5.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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FLIP (NT) peptide CATALOG NO.: 1159P SEQUENCE: SAEVIHQVEEALDTDEKE LOCATION: Amino acids 2 to 18 of human FLIP (1) APPLICATION:

The peptide is used for blocking the activity of anti-FLIP (catalog number 1159). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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FLIPα (CT) peptide CATALOG NO.: 1161P SEQUENCE: NGYMYDWNSRVSAKEKYY LOCATION: Amino acids 447 to 464 of human FLIP? (1) APPLICATION:

The peptide is used for blocking the activity of anti-FLIP? (catalog number 1161). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Daxx (CT) peptide CATALOG NO.: 1163P SEQUENCE: TSVATQCDPEEIIVLSDSD LOCATION: Amino acids 722 to 740 of human Daxx (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-Daxx (catalog number 1163). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel Fas-binding protein that activates

JNK and apoptosis. Cell 1997;89:1067-1076

2. Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd. Cloning and expression of primate Daxx

cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol 1997;16:1289-1298 5.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DR3 (NT) peptide CATALOG NO.: 1166P SEQUENCE: QGGTRSPRCDCAGDFH LOCATION: Amino acids 25 to 40 of human DR3 (1,2) APPLICATION:




Nature, 1996;384:372-5.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DR4 (NT) peptide CATALOG NO.: 1167P SEQUENCE: ASGTEAAAATPSKVWGSSAG LOCATION: Amino acids 1 to 20 of human DR4 (1) APPLICATION:



for the cytotoxic ligand TRAIL. Science; 1997;276:111-113


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Bonzo (CT) peptide CATALOG NO.: 1170P SEQUENCE: KSSEDNSKTFSASHNVEATS LOCATION: Amino acids 319 to 338 of human Bonzo (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-Bonzo (catalog number 1170). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by

simian and human immunodeficiency viruses. Nature 1997;388:296-300

2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine

receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J Exp Med 1997;185:2015-23.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ICAD (NT) peptide

CATALOG NO.: 2001P SEQUENCE: ELSRGASAPDPDDVRPLKPC LOCATION: Amino acids 2 to 21 of murine ICAD (1) APPLICATION:

The peptide is used for blocking the activity of anti-ICAD (catalog number 2001). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that

degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ICAD (CT) peptide

CATALOG NO.: 2003P SEQUENCE: -RRSPLPGEPQRPKRAKRDSS LOCATION: Amino acids 312 to 331 of murine ICAD (1) APPLICATION:

The peptide is used for blocking the activity of anti-ICAD (catalog number 2003). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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CAD (I17) peptide

CATALOG NO.: 2007P SEQUENCE: YNGSYFDRGAEASSRL LOCATION: Amino acids 205 to 222 of murine CAD (1) APPLICATION:

The peptide is used for blocking the activity of anti-CAD (catalog number 2007). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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CAD (CT) peptide

CATALOG NO.: 2011P SEQUENCE: KLECDRSRIYRPQTGS LOCATION: Amino acids 314 to 329 of murine CAD (1) APPLICATION:

The peptide is used for blocking the activity of anti-CAD (catalog number 2011). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Apaf-1 (NT) peptide

CATALOG NO.: 2013P SEQUENCE: HREALEKDIKTSYIMDHC LOCATION: Amino acids 12 to 28 of human Apaf-1 (1) APPLICATION:

The peptide is used for blocking the activity of anti-Apaf-1 (catalog number 2013). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans

CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Apaf-1 (CT) peptide

CATALOG NO.: 2015P SEQUENCE: TFYTNGTNLKKIHVSPDFKT LOCATION: Amino acids 1158 to 1177 of human Apaf-1 (1) APPLICATION:

The peptide is used for blocking the activity of anti-Apaf-1 (catalog number 2015). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans

CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DR5 (CT) peptide

CATALOG NO.: 2019P SEQUENCE: KIEDHLLSSGKFMYLEGNAD LOCATION: Amino acids 388 to 407 of human DR5 (1,2) APPLICATION:



1. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8

2. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL,

Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DcR2 (ID) peptide

CATALOG NO.: 2021P SEQUENCE: GGPERVHRVLFRRRS LOCATION: Amino acids 249 to 263 of human DcR2 (1-3) APPLICATION:

The peptide is used for blocking the activity of anti-DcR2 (catalog number 2021). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


for the cytotoxic ligand TRAIL. Science; 1997;276:111-113 2. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-

containing receptor for TRAIL. Science 1997;277:815-8 3. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL,

Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IKKα (C1) peptide

CATALOG NO.: 2025P SEQUENCE: CLGHLSTIIHEANEEQGNS LOCATION: Amino acids 716 to 734 of human IKKα (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-IKKα (catalog number 2025). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997;388:548-54

2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an

IkappaB kinase. Cell 1997;90:373-83


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ADAM10 (CT) peptide

CATALOG NO.: 2051P SEQUENCE: PQRQRPRESYQMGHMRR LOCATION: Amino acids 732 to 748 of human ADAM10 (1) APPLICATION:

The peptide is used for blocking the activity of anti-ADAM10 (catalog number 2051). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis

factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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FLIPs (CT) peptide

CATALOG NO.: 2055P SEQUENCE: QKSLKDPSNNFRMITPYAH LOCATION: Amino acids 191 to 209of human FLIPs (1) APPLICATION:

The peptide is used for blocking the activity of anti-FLIPs (catalog number 2055). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,

Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP.

Nature 1997;388:190-195


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

DNase II (CT) peptide CATALOG NO.: 2059P SEQUENCE: NH2-CNGMARKPSRAYKI-OH LOCATION: Amino acids 347 to 360 of human DNase II precursor (1-3). APPLICATION:

The peptide is used for blocking the antibody activity of DNase II (catalog number 2059). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:

It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE:

1. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6

2. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14

3. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Bim (IN) peptide

CATALOG NO.: 2065P SEQUENCE: AERPPQLRPGAPTSLQTEP LOCATION: Amino acids 22 to 40 of human Bim (1) APPLICATION:

The peptide is used for blocking the activity of anti-Bim (catalog number 2065). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. O'Connor,L., Strasser,A., O'Reilly,L.A., Hausmann,G., Adams,J.M., Cory,S. and Huang,D.C. Bim: a

novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998;17:384-395


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ZIPK (IN) peptide

CATALOG NO.: 2067P SEQUENCE: RRRNVRGEDSGRKPERRRLK LOCATION: Amino acids 279 to 298 of human ZIPK (1) APPLICATION:

The peptide is used for blocking the activity of anti-ZIPK (catalog number 2067). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which

mediates apoptosis. Mol Cell Biol 1998;18:1642-51


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Caspase-9 (IN1) peptide

CATALOG NO.: 2071P SEQUENCE: EDIQRAGSGSRRDQAR LOCATION: Amino acids 41 to 56 of human caspase-9 (1-3) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel

member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA,

Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and

dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Caspase-9 (IN2) peptide

CATALOG NO.: 2073P SEQUENCE: ASTSPEDESPGSNPEPDATP LOCATION: Amino acids 299 to 318 of human caspase-9 (1-3) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel

member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA,

Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and

dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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RICK (NT) peptide

CATALOG NO.: 2075P SEQUENCE: PTIPYHKLADLRYLSRGASG LOCATION: Amino acids 11 to 30 of human RICK (1) APPLICATION:

The peptide is used for blocking the activity of anti-RICK (catalog number 2075). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase

recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem 1998;273:12296-300


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ARC (CT) peptide

CATALOG NO.: 2081P SEQUENCE: PDPEPEPDFEERDESEDS LOCATION: Amino acids 191 to 208 of human ARC (1) APPLICATION:

The peptide is used for blocking the activity of anti-ARC (catalog number 2081). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle

and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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CIDE-A (CT) peptide

CATALOG NO.: 2085P SEQUENCE: DDKEERPSLRSQAKGRFT LOCATION: Amino acids 200 to 217 of human CIDE-A (1) APPLICATION:

The peptide is used for blocking the activity of anti-CIDE-A (catalog number 2085). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with

homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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mCIDE-A (CT) peptide

CATALOG NO.: 2089P SEQUENCE: GDTEEQPSPKPSTKG LOCATION: Amino acids 200 to 214 of murine CIDE-A (1) APPLICATION:

The peptide is used for blocking the activity of anti-mCIDE-A (catalog number 2089). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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mCIDE-B (CT) peptide

CATALOG NO.: 2091P SEQUENCE: EGADRWQWHGQRHLHS LOCATION: Amino acids 204 to 219 of murine CIDE-B (1) APPLICATION:

The peptide is used for blocking the activity of anti-mCIDE-B (catalog number 2091). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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CX3CR1 (NT) peptide

CATALOG NO.: 2093P SEQUENCE: DQFPESVTENFEYDDLAEAC LOCATION: Amino acids 2 to 21 of murine CX3CR1 (1) APPLICATION:

The peptide is used for blocking the activity of anti-CX3CR1 (catalog number 2093). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled

receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995;163:295-9

2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization, and RNA expression of a

human beta chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CCR8 (EL) peptide

CATALOG NO.: 2097P SEQUENCE: CYSFYNQQTLKWKIFTNFK LOCATION: Amino acids 732 to 748 of human CCR8 (1-3) APPLICATION:

The peptide is used for blocking the activity of anti-CCR8 (catalog number 2097). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A, Storlazzi CT, Rocchi M, Santoni A. Molecular cloning of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J Immunol 1996;157:2759-63

2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G. Molecular cloning and RNA expression of two

new human chemokine receptor-like genes. Biochem Biophys Res Commun 1996;227:846-53 3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G, Parmentier M. Molecular cloning and

chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor. Eur J Immunol 1996;26:3021-8


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GPR15 (NT) peptide

CATALOG NO.: 2101P SEQUENCE: YYATSPNSDIRETHSH LOCATION: Amino acids 13 to 28 of murine GPR15 (1) APPLICATION:

The peptide is used for blocking the activity of anti-GPR15 (catalog number 2101). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Heiber M, Marchese A, Nguyen T, Heng HH, George SR, O'Dowd BF. A novel human gene encoding

a G-protein-coupled receptor (GPR15) is located on chromosome 3. Genomics 1996;32:462-5


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DFF40/CAD (I18) peptide

CATALOG NO.: 2107P SEQUENCE: EGLESRFRNKSGYLRYS LOCATION: Amino acids 147 to 164 of murine CAD (1) APPLICATION:

The peptide is used for blocking the activity of anti-DFF40/CAD (catalog number 2107). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.




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IKKα (C2) peptide

CATALOG NO.: 2115P SEQUENCE: TPQDGETSAQMIEENLN LOCATION: Amino acids 699 to 715 of human IKKα (1-3) APPLICATION:







Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IKKα (C3) peptide

CATALOG NO.: 2117P SEQUENCE: CTQSSARSLVGSSLEGA LOCATION: Amino acids 658 to 674 of human IKKα (1-3) APPLICATION:







Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IKKβ (C3) peptide CATALOG NO.: 2121P SEQUENCE: CSKVRGPVSGSPDSMNASR LOCATION: Amino acids 662 to 680 of human IKKβ (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-IKKβ (catalog number 2121). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK)

contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997;91:243-52

2. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-beta: NF-kappaB activation and

complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9


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IRAK2 (C2) peptide CATALOG NO.: 2123P SEQUENCE: APWAGAATPLLPTENGEGR LOCATION: Amino acids 546 to 564 of human IRAK2 (1) APPLICATION:

The peptide is used for blocking the activity of anti-IRAK2 (catalog number 2123). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal

mediators of IL-1 signaling. Science 1997;278:1612-5


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MyD88 (IN) peptide CATALOG NO.: 2125P SEQUENCE: CDFQTKFALSLSPGAHD LOCATION: Amino acids 233 to 248 of human MyD88 (1) APPLICATION:

The peptide is used for blocking the activity of anti-MyD88 (catalog number 2125). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE. The cloning and characterization of

human MyD88: amember of an IL-1 receptor related family. FEBS Lett 1997;402:81-4


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MyD88 (CT) peptide CATALOG NO.: 2127P SEQUENCE: CTKSWFWTRLAKALSLP LOCATION: Amino acids 279 to 296 of human MyD88 (1) APPLICATION:

The peptide is used for blocking the activity of anti-MyD88 (catalog number 2127). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE. The cloning and characterization of

human MyD88: a member of an IL-1 receptor related family. FEBS Lett 1997;402:81-4


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IL-1RAcP (C2) peptide CATALOG NO.: 2129P SEQUENCE: KWKGEKSKYPQGRFWK LOCATION: Amino acids 525 to 540 of human IL-1RAcP (1) APPLICATION:

The peptide is used for blocking the activity of anti-IL-1RAcP (catalog number 2129). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and

characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-65


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IL-1RAcP (CT) peptide CATALOG NO.: 2131P SEQUENCE: KKSPRRSSSDEQGLSYSSLKN LOCATION: Amino acids 549 to 569 of human IL-1RAcP (1) APPLICATION:

The peptide is used for blocking the activity of anti-IL-1RAcP (catalog number 2131). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and

characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-65


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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APP (AβNT) peptide CATALOG NO.: 2133P SEQUENCE: DAEFRHDSGY LOCATION: Amino acids 653 to 662 of human APP (1) APPLICATION:

The peptide is used for blocking the activity of anti-APP (catalog number 2133). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W,

Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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APP (CT) peptide CATALOG NO.: 2136P SEQUENCE: GYENPTYKFFEQMQN LOCATION: Amino acids 737 to 751 of human APP (1) APPLICATION:

The peptide is used for blocking the activity of anti-APP (catalog number 2136). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W,

Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DcR3 (NT) peptide CATALOG NO.: 2140P SEQUENCE: AETPTYPWRDAETGERC LOCATION: Amino acids 31 to 46 of human DcR3 (1,2) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, Huang A, Donahue CJ, Sherwood SW,

Baldwin DT, Godowski PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD, Botstein D, Ashkenazi A. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699-703

2. Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly identified member of tumor necrosis factor receptor

superfamily (TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem 1999;274:13733-6


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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SODD (NT) peptide CATALOG NO.: 2143P SEQUENCE: SALRRSGYGPSDGPSC LOCATION: Amino acids 2 to 16 of human SODD (1) APPLICATION:

The peptide is used for blocking the activity of anti-SODD (catalog number 2143). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of constitutive TNF receptor 1 signaling by

silencer of death domains. Science 1999;283:543-6


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DRAK1 (NT) peptide CATALOG NO.: 2147P SEQUENCE: EKPGSGGSSPGATSGC LOCATION: Amino acids 5 to 19 of human DRAK1 (1) APPLICATION:

The peptide is used for blocking the activity of anti-DRAK1 (catalog number 2147). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein

kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71


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DRAK2 (CT) peptide CATALOG NO.: 2149P SEQUENCE: CSKRFRFDDSLPNPHE LOCATION: Amino acids 351 to 365 of human DRAK2 (1) APPLICATION:

The peptide is used for blocking the activity of anti-DRAK2 (catalog number 2149). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein

kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DFF40 (NT) peptide CATALOG NO.: 2153P SEQUENCE: QKPKSVKLRALRSPRKC LOCATION: Amino acids 3 to 18 of human DFF40 (1,2) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA

fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

2. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and

characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DFF40 (IN) peptide CATALOG NO.: 2155P SEQUENCE: NGSYFDRGAKGGSRLC LOCATION: Amino acids 203 to 218 of human DFF40 (1,2) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA

fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

2. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and

characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DR6 (NT) peptide CATALOG NO.: 2157P SEQUENCE: QPEQKASNLIGTYRHC LOCATION: Amino acids 42 to 56 of human DR6 (1) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C, Aggarwal BB, Ni J, Dixit VM.

Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett 199;431:351-6


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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BCL-10 (NT) peptide CATALOG NO.: 2161P SEQUENCE: APSLTEEDLTEVKKDC LOCATION: Amino acids 5 to 19 of human BCL-10 (1-5) APPLICATION:

The peptide is used for blocking the activity of anti-BCL-10 (catalog number 2161). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and

mutated in multiple tumor types. Cell 1999;96(1):35-45 2. Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF kappaB-activating protein containing a caspase

recruitment domain with homology to Herpesvirus-2 protein E10. J Biol Chem 1999;274(15):9955-61 3. Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel caspase recruitment domain-containing

proapoptotic molecule. J Biol Chem 1999;274(15):10287-92 4. Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2 E10 gene product, but not its cellular homologue,

activates NF-kappaB transcription factor and c-Jun N-terminal kinase. J Biol Chem 1999;274(15):9962-8 5. Srinivasula SM, Ahmad M, Lin JH, et al. CLAP, a novel caspase recruitment domain-containing protein in the

tumor necrosis factor receptor pathway, regulates NF-kappaB activation and apoptosis. J Biol Chem 1999;274(25):17946-54


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DcR1 (ED) peptide CATALOG NO.: 2179P SEQUENCE: CVEEFGANATVETPAAEET LOCATION: Amino acids 149 to 167 of human DcR1 (1) APPLICATION:


For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.. REFERENCE: 1. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for

TRAIL. Science 1997;277:815-8 2. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of

signaling and decoy receptors. Science 1997;277:818-21


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RICK (CT) peptide

CATALOG NO.: 2183P SEQUENCE: KLKDNKQMGLQPYPE LOCATION: Amino acids 508 to 522 of human RICK (1) APPLICATION:

The peptide is used for blocking the activity of anti-RICK (catalog number 2183). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase

recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem 1998;273:12296-300


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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ARC (NT) peptide

CATALOG NO.: 2185P SEQUENCE: GNAQERPSETIDRERKR LOCATION: Amino acids 2 to 18 of human ARC (1) APPLICATION:

The peptide is used for blocking the activity of anti-ARC (catalog number 2185). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle

and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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CX3CR1 (EL) peptide

CATALOG NO.: 2201P SEQUENCE: CLGDYPEVLQEIWPV LOCATION: Amino acids 175 to 189 of human CX3CR1/V28 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-CX3CR1 (catalog number 2201). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled

receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995;163:295-9

2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization, and RNA expression of a

human beta chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Bonzo (NT2) peptide

CATALOG NO.: 2209P SEQUENCE: GFSSFNDSSQEEHQD LOCATION: Amino acids 11 to 25 of human Bonzo/STRL33 (1,2) APPLICATION:

The peptide is used for blocking the activity of anti-Bonzo (catalog number 2209). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.


1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by

simian and human immunodeficiency viruses. Nature 1997;388:296-300

2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine

receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J Exp Med 1997;185:2015-23


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IRAK2 (CT) peptide CATALOG NO.: 2213P SEQUENCE: READSSSEACVGLEPPQDVT LOCATION: Amino acids 571 to 590 of human IRAK2 (1) APPLICATION:

The peptide is used for blocking the activity of anti-IRAK2 (catalog number 2213). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal

mediators of IL-1 signaling. Science 1997;278:1612-5


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Acinus (CP) peptide CATALOG NO.: 2215P SEQUENCE: NH2-TRTALHGVKWPQSNPK-OH LOCATION: Amino acids 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS’, or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17. APPLICATION:

The peptide is used for blocking the antibody activity of Acinus (catalog number 2215). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Acinus (CT) peptide CATALOG NO.: 2217P SEQUENCE: NH2-SRSTPVRDRGGRR-OH LOCATION: Amino acids 1329 to 1341 of human AcinusL, 602 to 614 of human AcinusS’, or 571 to 583 of human AcinusS (1). Application:



STORAGE:




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Acinus (IN) peptide CATALOG NO.: 2219P SEQUENCE: NH2-DTSENRPENDVPEPP-OH LOCATION: Amino acids 775 to 789 of human AcinusL, 48 to 62 of human AcinusS’, or 17 to 31 of human AcinusS (1). APPLICATION:



STORAGE:




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

BAFF (CT) peptide CATALOG NO.: 2221P SEQUENCE: NH2-EEGDELQLAIPRENAQ-OH LOCATION: Amino acids 254 to 269 of human BAFF/BLys (1,4) APPLICATION:

The peptide is used for blocking the activity of BAFF (catalog number 2221). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3 2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747-56 3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol 1999;65:680-3 4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81


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APRIL (ED) (TALL-2/TRDL-1) peptide

CATALOG NO.: 2223P SEQUENCE: NH2-GTGGPSQNGEGYP-OH LOCATION: Amino acids 67 to 79 of human APRIL (1-3). APPLICATION:

The peptide is used for blocking the antibody activity of APRIL (catalog number 2423). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.

2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-

regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3. 3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand,

stimulates cell death. Cancer Res. 2000;60(4):1021-7.


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DEDAF peptide

CATALOG NO.: 2227P SEQUENCE: NH2-TPKGDMSAVNDESF-OH LOCATION: Amino acids 215 to 228 of human DADEF (1) APPLICATION:

The peptide is used for blocking the activity of DEDAF (catalog number 2227). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Zheng L, Schickling O, Peter ME, Lenardo MJ. The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 2001;276(34):31945-52.


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Survivin (NT) peptide

CATALOG NO.: 2233P SEQUENCE: NH2-MGAPTLPPAWQP-OH LOCATION: Amino acids 1 to 12 of human survivin (1). APPLICATION:

The peptide is used for blocking the antibody activity of survivin (catalog number 2233). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-21


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Survivin (CT) peptide CATALOG NO.: 2235P SEQUENCE: NH2-KKVRRAIEQLAAMD-OH LOCATION: Amino acids 129 to 142 of human survivin (1). APPLICATION:



STORAGE:


1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-21


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Survivin (CT) peptide

CATALOG NO.: 2237P SEQUENCE: NH2-AKTTRQSIEQLAA-OH LOCATION: Amino acids 128 to 140 of mouse survivin (1). APPLICATION:



STORAGE:


1. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T. Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation. Proc Natl Acad Sci USA 1999;96:1457-62


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AIF (NT) peptide CATALOG NO.: 2239P SEQUENCE: NH2-KQKKAALSASEGEE-OH LOCATION: Amino acids 109 to 122 of human AIF (1). APPLICATION:

The peptide is used for blocking the antibody activity of AIF (catalog number 2239). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6


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Acinus (NP) peptide CATALOG NO.: 2241P SEQUENCE: NH2-DDPVRTAQVPSPPRGK-OH LOCATION:

Amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS’, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is located near the N-terminus of active peptide p17. APPLICATION:



STORAGE:




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Caspase-13 peptide CATALOG NO.: 2245P SEQUENCE: NH2-ETVAGPDESVGSAAT-OH LOCATION: Amino acids 91 to 105 of human Acaspase-13 (1). APPLICATION:

The peptide is used for blocking the antibody activity of caspase-13 (catalog number 2245). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem 1998;273:15702-7


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BACE2 (NT) peptide CATALOG NO.: 2247P SEQUENCE: NH2-APTPGPGTPAERHADG-OH LOCATION: Amino acids 44 to 59 of human BACE2 (1). APPLICATION:

The peptide is used for blocking the antibody activity of BACE2 (catalog number 2247). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999;402:533-7


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BACE2 (CT) peptide

CATALOG NO.: 2249P SEQUENCE: NH2-CQRRPRDPEVVNDESS-OH LOCATION: Amino acids 496 to 511 of human BACE2 (1). APPLICATION:

The peptide is used for blocking the antibody activity of BACE2 (catalog number 2249). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999;402:533-7


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BACE (CT) peptide CATALOG NO.: 2253P SEQUENCE: NH2-CLRQQHDDFADDISLLK-OH LOCATION: Amino acids 485 to 501 of human BACE (1). APPLICATION:

The peptide is used for blocking the antibody activity of BACE (catalog number 2253). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41


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AIF (IN) peptide CATALOG NO.: 2267P SEQUENCE: NH2-PKSATEQSGTGIRSE-OH LOCATION:

Amino acids 517 to 531 of human AIF, which are identical to corresponding amino acids of mouse and rat AIF (1). APPLICATION:



STORAGE:


1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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FLASH (CT) peptide

CATALOG NO.: 2271P SEQUENCE: NH2-SERFQQLMKLFEKSKC-OH LOCATION: Amino 1253 to 1268 of human FLASH (1). APPLICATION:

The peptide is used for blocking the antibody activity of FLASH (catalog number 2271). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3


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F1Aα peptide CATALOG NO.: 2279P SEQUENCE: NH2-DINYQDQIPRTLEE-OH LOCATION: Amino acids 609 to 622 of human F1Aα, ???which are identical to the corresponding sequences of mouse FEM1β (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of F1Aα (catalog number 2279). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8. 2. Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20


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RIP3 peptide

CATALOG NO.: 2283P SEQUENCE: NH2-AQFGRGRGWQPFHK-OH LOCATION: Amino acids 473 to 486 of murine RIP3 (1). APPLICATION:

The peptide is used for blocking the antibody activity of RIP3 (catalog number 2283). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-? B. Mol Cell Biol. 1999;19(10):6500-8.


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ASC peptide

CATALOG NO.: 2287P SEQUENCE: NH2-RESQSYLVEDLERS-OH LOCATION: Amino acids 182 to 195 of human ASC (1). APPLICATION:

The peptide is used for blocking the antibody activity of ASC (catalog number 2287). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8.


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Bnip3L (IN) peptide

CATALOG NO.: 2289P SEQUENCE: NH2-DAQHESGQSSSRGSSH-OH LOCATION: Amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L (1). APPLICATION:

The peptide is used for blocking the antibody activity of Bnip3L (catalog number 2289). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10


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DcR1 (ED2) peptide CATALOG NO.: 2299P SEQUENCE: NH2-CKEGTFRNENSPE-OH LOCATION: Amino acids 111 to 123 at extracellular domain of human DcR1 precursor (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of DcR1 (catalog number 2299). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8 3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21


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AIF (CT) peptide

CATALOG NO.: 2301P SEQUENCE: NH2-KDGEQHEDLNEVAK-OH LOCATION: Amino acids 593 to 606 of human AIF (1). APPLICATION:


For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DAP Kinase 2 peptide

CATALOG NO.: 2323P SEQUENCE: NH2-ARRKALHPRRRSSTS-OH LOCATION: Amino acids 356 to 370 of human DAPK2 (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of DAP kinase 2 (catalog number 2323). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80 2. Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Caspase-12 (NT) peptide

CATALOG NO.: 2325P SEQUENCE: NH2-AARRTHERDPIYKIKG-OH LOCATION: Amino acids 2 to 17 of murine caspase-12 (1). APPLICATION:


For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-103.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Caspase-12 (IN) peptide

CATALOG NO.: 2327P SEQUENCE: NH2-CTPSSPSESKRKVEDDE-OH LOCATION: Amino acids 100 to 116 of murine caspase-12 (1). APPLICATION:


For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-103.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IKKε/IKK-i (CT) peptide CATALOG NO.: 2329P SEQUENCE: NH2-NRIIERLNRVPAPPDV-OH LOCATION: Amino acids 701 to 716 of human IKKε/IKK-i (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of IKKε/IKK-i (catalog number 2329). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Peters RT, Liao SM, Maniatis T. IKKepsilon is part of a novel PMA-inducible IkappaB kinase complex. Mol Cell 2000;5(3):513-22 2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int Immunol 1999;11(8):1357-62


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IKKγ/NEMO (NT) peptide

CATALOG NO.: 2335P SEQUENCE: NH2-CQYQAPDMDTLQIHVME-OH LOCATION: Amino acids 400 to 416 of human IKKγ (1,2) APPLICATION:

The peptide is used for blocking the antibody activity of IKKγ (catalog number 2335). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature 1998;395(6699):297-300 2. Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation. Cell. 1998;93(7):1231-40.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DC-SIGN (CT) peptide

CATALOG NO.: 2347P SEQUENCE: NH2-CSRDEEQFLSPAPATPNPPPA-OH LOCATION: Amino acids 384 to 404 of human DC-DIGN (1). Application:

The peptide is used for blocking the antibody activity of DC-SIGN (catalog number 2347). It usually blocks the antibody activity completely in Western blot by incubating the peptide with an equal volume of antibody for 30 min at 37°C


STORAGE:


1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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DC-SIGN (ED) peptide

CATALOG NO.: 2349P SEQUENCE: NH2-CYFMSNSQRNWHDSITA-OH LOCATION: Amino acids 277 to 293 of human DC-DIGN (1). Application:

The peptide is used for blocking the antibody activity of DC-SIGN (catalog number 2347). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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NAK/TBK1 (CT) peptide

CATALOG NO.: 2351P

SEQUENCE: NH2-ERFGSLTMDGGLRNVD-OH

LOCATION: Amino acids 712 to 727 of human NAK/TBK1 (1,2)

APPLICATION:

The peptide is used for blocking the antibody activity of NAK/TBK1 (catalog number 2351). It usually

blocks the antibody activity completely in Western blot by incubating the peptide with an equal volume of

antibody for 30 min at 37°C


STORAGE:

It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM

NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.

REFERENCE: 1. Pomerantz JL, Baltimore D. NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704

2. Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y,

Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an IkappaB kinase-activating kinase. Nature

2000;404(6779):778-82


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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IRAK-M peptide

CATALOG NO.: 2355P SEQUENCE: NH2-CSSKFSWDEYEQYKKE-OH LOCATION: Amino acids 581 to 596 of human IRAK-M (1) APPLICATION:

The peptide is used for blocking the antibody activity of IRAK-M (catalog number 2255). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J Biol Chem. 1999;274(27):19403-10.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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p53R2 (NT) peptide CATALOG NO.: 2383P SEQUENCE: NH2-GDPERPEAAGLDQDER-OH LOCATION: Amino acids 2 to 17 of human p53R2 (1) APPLICATION:

The peptide is used for blocking the antibody activity of p53R2 (catalog number 2383). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Chk2 (NT) peptide CATALOG NO.: 2391P SEQUENCE: NH2-SRESDVEAQQSHGSSAC-OH LOCATION: Amino acids 2 to 18 of human Chk2 (1) APPLICATION:

The peptide is used for blocking the antibody activity of Chk2 (catalog number 2391). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

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Smac/DIABLO (CT) peptide

CATALOG NO.: 2409P SEQUENCE: NH2-EERAESEQEAYLRED-OH LOCATION: Amino acids 225 to 239 of human Smac/DIABLO (1) APPLICATION:

The peptide is used for blocking the antibody activity of mSmac/DIABLO (catalog number 2409). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell. 2000;102(1):33-42.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

mSmac/DIABLO (CT) peptide

CATALOG NO.: 2411P SEQUENCE: NH2- SDEGADQEEEAYLRED-OH LOCATION: Amino acids 222 to 237 of murine Smac/DIABLO (1) APPLICATION:

The peptide is used for blocking the antibody activity of mSmac/DIABLO (catalog number 2411). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000;102(1):43-53.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

APRIL (ED2) (TALL-2/TRDL-1) peptide

CATALOG NO.: 2415P SEQUENCE: NH2-CIRSMPSHPDRAYNS-OH LOCATION: Amino acids 196 to 210 of human APRIL (1-3). Application:

The peptide is used for blocking the antibody activity of APRIL (catalog number 2415). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.

2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-

regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3. 3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand,

stimulates cell death. Cancer Res. 2000;60(4):1021-7.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

PID/MTA2 peptide CATALOG NO.: 2443P SEQUENCE: PAPSHPASTNEPIVLED LOCATION: Amino acids 652 to 668 of human PID/MTA2 (1) APPLICATION:

The peptide is used for blocking the activity of PID/MTA2 (catalog number 2443). Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.

For research use only. STORAGE: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year. REFERENCE: 1. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its effect on cell growth and

apoptosis. Nature. 2000;408:377-81.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

MTBP (IN2) peptide

CATALOG NO.: 2447P SEQUENCE: NH2-GAVECFEEEDSNSRESLS-OH LOCATION: Amino acids 122 to 139 of human MTBP (1). APPLICATION:

The peptide is used for blocking the antibody activity of MTBP (catalog number 2247). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

PERP peptide

CATALOG NO.: 2451P

SEQUENCE: NH2-EDDLLGNAKPRYFYTSA-OH

LOCATION: Amino acids 175 to 193 of human PERP (1,2).

Application:

The peptide is used for blocking the antibody activity of PERP (catalog number 2451). It can

block the antibody activity completely in Western blot by incubating the peptide with equal volume

of antibody for 30 min at 37°C


STORAGE:

It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130

mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,


REFERENCE:

1. Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and

induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.

2. Attardi,L.D., Reczek,E.E., Cosmas,C., et al. PERP, an apoptosis-associated target of p53,

is a novel member of the PMP-22/gas3 family. Genes Dev. 2000;14 (6):704-718


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IL-21 (IN) peptide

CATALOG NO.: 2465P SEQUENCE: NH2-TCPSCDSYEKKPPKE-OH LOCATION: Amino acids 121 to 135 of human IL-21 precursor (1). APPLICATION:

The peptide is used for blocking the antibody activity of IL-21 (catalog number 2465). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Arrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IL-21R (ED) peptide

CATALOG NO.: 2469P SEQUENCE: NH2-NITDQSGNYSQECGS-OH LOCATION: Amino acids 97 to 111 of human IL-21R precursor (1). APPLICATION:

The peptide is used for blocking the antibody activity of IL-21R (catalog number 2469). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IL-21R (NT) peptide

CATALOG NO.: 2471P SEQUENCE: NH2-CPDLVCYTDYLQT-OH LOCATION: Amino acids 20 to 32 of human IL-21R precursor (1). APPLICATION:



STORAGE:




Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IL-22R (CT) peptide

CATALOG NO.: 2489P SEQUENCE: NH2-DSLFRGLALTVQWES-OH LOCATION: Amino acids 560 to 574 of human IL-22R precursor (1). APPLICATION:



STORAGE:


1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

IL-22R (NT) peptide

CATALOG NO.: 2497P SEQUENCE: NH2-QSSNFENILTWDSGPE-OH LOCATION: Amino acids 31 to 46 of human IL-22R precursor (1). APPLICATION:



STORAGE:


1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CIKS/Act1 (CT) peptide

CATALOG NO.: 2499P SEQUENCE: NH2-REEEYVAPPRGPLPT-OH LOCATION: Amino acids 554 to 568 of human CIKS (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of CIKS/Act1 (catalog number 2499). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Ikappa B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.

2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR.

Act1, an NF-kappa B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

CIKS/Act1 (NT) peptide

CATALOG NO.: 2501P SEQUENCE: NH2-PPQLQETRMNRSIP-OH LOCATION: Amino acids 2 to 15 of human CIKS (1,2). APPLICATION:

The peptide is used for blocking the antibody activity of CIKS/Act1 (catalog number 2501). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C


STORAGE:


1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Ikappa B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.

2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR.

Act1, an NF-kappa B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.

lysate Data Sheets


Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


HeLa Cell Lysate Catalog No: 1201

Cell line: HeLa-S3 (Human cervix epithelioid carcinoma)

Growth media: Jokliks-MEM and 5% NCS (Newborn calf serum).

Cell lysate preparation:

HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium

chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM

phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium

dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by

centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate

was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%

sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Application:

HeLa cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is

recommended for mini gel.

Storage:

It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%

β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.



Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


A431 Cell Lysate Catalog No: 1202

Cell line: A431 (Human epidermoid carcinoma)

Growth media: DMEM and 10% FBS.


A431 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

A431 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


A549 Cell Lysate Catalog No: 1203

Cell line: A549 (Human lung carcinoma)

Growth media: DMEM and 10% NCS (Newborn calf serum)


A549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

A549 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


K562 Cell Lysate Catalog No: 1204

Cell line: K562 (Human chronic myelogenous leukemia)

Growth media: RPMI & 10% NCS (Newborn calf serum)


K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

K562 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Jurkat Cell Lysate Catalog No: 1205

Cell line: Jurkat, clone E6-1 (Human acute T cell leukemia)



Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

Jurkat cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


MOLT4 Cell Lysate Catalog No: 1206

Cell line: MOLT4 (Human acute lymphoblastic leukemia)

Growth media: RPMI and 10% FBS


MOLT4 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

MOLT4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Raji Cell Lysate Catalog No: 1207

Cell line: Raji (Human Burkitt's lymphoma)



Raji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

Raji cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


THP-1 Cell Lysate Catalog No: 1208

Cell line: THP-1 (Human monocyte)

Growth media: RPMI & 10% FBS


THP-1 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

THP-1 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


HL60 Cell Lysate Catalog No: 1209

Cell line: HL60 (Human promyelocytic leukemia)

Growth media: RPMI & 10% FBS (Fetal bovine serum)


HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


293 Cell Lysate Catalog No: 1210

Cell line: 293 (Human transformed primary embryonal kidney)

Growth media: RPMI & 10% FBS (Fetal bovine serum)


293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:

It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing

5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.



Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


HepG2 Cell Lysate Catalog No: 1211

Cell line: HepG2 (Human hepatocellular carcinoma)

Growth media: DMEM & 10% FBS (Fetal bovine serum)


HepG2 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium







Application:

HepG2 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


3T3 Cell Lysate Catalog No: 1212

Cell line: BALB/3T3 clone A31 (Mouse embryo)



BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer

(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM






Application:

BALB/3T3 clone A31 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg

per lane is recommended for mini gel.

Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


T24 Cell Lysate Catalog No: 1213

Cell line: T24 (Transitional-cell human bladder carcinoma)



T24 (Transitional-cell human bladder carcinoma) cell lysate was prepared by homogenization

in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM

ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1%

sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin).

Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad

protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH

6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-

mercaptoethanol.

Application:

T24 (Transitional-cell human bladder carcinoma) cell lysate is ready to load on SDS-PAGE

for Western blotting, 20 µg per lane is recommended for mini gel.

Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


SW1353 Cell Lysate Catalog No: 1214

Cell line: SW1353 (Human humerus chondrosarcoma)



SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM

sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM






Application:

SW1353 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


U937 Whole Cell Lysate Catalog No: 1215

Cell line: U937 (Human histiocytic lymphoma)

Growth media: DMEM & 10% NCS (Newborn Calf serum)


U937 (Human histiocytic lymphoma) cell lysate was prepared by homogenization in modified

RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic

acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1%

sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by




Application:

U937 (Human histiocytic lymphoma) cell lysate is ready to load on SDS -PAGE for Western

blotting, 20 µg per lane is recommended for mini gel.

Storage:

It is supplied as whole cell lysate, 200 µg per vial at 100 µg at 2 mg/ml, in 1 x SDS sample

buffer containing 5% β-mercaptoethanol. Storage at -20°C will be stable for three months, storage

at –70°C will be stable for one year.



Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


PC-3 Whole Cell Lysate Catalog No: 1216

Cell line: PC-3 (Human prostate adenocarcinoma)

Growth media: DMEM/Hams F12 & 10% NCS (Newborn Calf serum)


PC-3 (Human prostate adenocarcinoma) cell lysate was prepared by homogenization in

modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM






mercaptoethanol.

Application:

PC-3 (Human prostate adenocarcinoma) cell lysate is ready to load on SDS-PAGE for

Western blotting, 20 µg per lane is recommended for mini gel.

Storage:






Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


MDA-MB-361 Whole Cell Lysate Catalog No: 1217

Cell line: MDA-MB-361 (Human breast adenocarcinoma)

Growth media: DMEM/Hams F12 & 10% NCS (Newborn Calf serum)


MDA-MB-361 (Human breast adenocarcinoma) cell lysate was prepared by homogenization

in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM






mercaptoethanol.

Application:

MDA-MB-361 (Human breast adenocarcinoma) cell lysate is ready to load on SDS-PAGE for

Western blotting, 20 µg per lane is recommended for mini gel.

Storage:






Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Heart Tissue Lysate Catalog No: 1301

Source: 38 year old female

This material has been tested by accepted techniques and has been found to be negative

for HBsAg, HIV 1/2, and HCV.

Tissue lysate preparation:

Human heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM



dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by

centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue

lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%


Application:

Human heart tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane

is recommended for mini gel.

Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Lung Tissue Lysate Catalog No: 1302

Source: 38-year-old female




Human lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Human lung tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Brain Tissue Lysate Catalog No: 1303





Human brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Human brain tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Liver Tissue Lysate Catalog No: 1304





Human liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Human liver tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Kidney Tissue Lysate Catalog No: 1305





Human kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150

mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM






Application:

Human kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per

lane is recommended for mini gel.

Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Spleen Tissue Lysate Catalog No: 1306





Human spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150







Application:

Human spleen tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Pancreas Tissue Lysate Catalog No: 1307





Human pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150







Application:

Human pancreas tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Small Intestine Tissue Lysate Catalog No: 1308





Human small intestine tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Human small intestine tissue lysate is ready to load on SDS -PAGE for Western blotting, 20

µg per lane is recommended for mini gel.

Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692

[email protected]

Human Lung Tumor Tissue Lysate Catalog No: 1321





Human lung tumor (adenocarcinoma) tissue lysate was prepared by homogenization in lysis

buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid,

10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was

removed by centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50

mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue)

containing 5% β-mercaptoethanol.

Application:

Human lung tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Brain Tumor Tissue Lysate Catalog No: 1322





Human brain tumor (Meningeal tumor) tissue lysate was prepared by homogenization in lysis

buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml aprotinin, 5

µg/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS). Tissue and cell debris was removed by

centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl

pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-

mercaptoethanol.

Application:

Human brain tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Breast Tumor Tissue Lysate Catalog No: 1323





Human breast tumor (moderately differentiated invasive ductal carcinoma) tissue lysate was

prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1

mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease

inhibitors). Tissue and cell debris was removed by centrifugation. The tissue lysate was boiled for

5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium

dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Application:

Human breast tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Kidney Tumor Tissue Lysate Catalog No: 1324





Human kidney tumor (renal cell carcinoma) tissue lysate was prepared by homogenization in

lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic

acid, 10% glycerol, 1% NP -40, and a cocktail of protease inhibitors). Tissue and cell debris was

removed by centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50

mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue)

containing 5% β-mercaptoethanol.

Application:

Human kidney tumor tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Colon Normal Tissue Lysate Catalog No: 1341-N

Source: Human adult colon




Human colon normal tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Human colon normal tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg


Storage:

It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer

containing 5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.



Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Colon Tumor Tissue Lysate Catalog No: 1341-T

Source: Human adult colon




Human colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Human colon tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Liver Normal Tissue Lysate Catalog No: 1342-N

Source: Human adult liver




Human liver normal tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Human liver normal tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Human Liver Tumor Tissue Lysate Catalog No: 1342-T

Source: Human adult liver




Human liver tumor tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Human liver tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Heart Tissue Lysate Catalog No: 1401

Source: White laboratory mice


Mouse heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Mouse heart tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Lung Tissue Lysate Catalog No: 1402



Mouse lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Mouse lung tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Brain Tissue Lysate Catalog No: 1403



Mouse brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Mouse brain tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Liver Tissue Lysate Catalog No: 1404



Mouse liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Mouse liver tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Kidney Tissue Lysate Catalog No: 1405



Mouse kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150







Application:

Mouse kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Spleen Tissue Lysate Catalog No: 1406



Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150







Application:

Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Skeletal Muscle Tissue Lysate Catalog No: 1407



Mouse skeletal muscle tissue lysate was prepared by homogenization in modified RIPA

buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid,

1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium





Application:

Mouse skeletal muscle lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Mouse Small Intestine Tissue Lysate Catalog No: 1408



Mouse small intestine tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Mouse small intestine tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Heart Tissue Lysate Catalog No: 1461

Source: White laboratory rats


Rat heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat heart tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Lung Tissue Lysate Catalog No: 1462



Rat lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat lung tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Brain Tissue Lysate Catalog No: 1463



Rat brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat brain tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Liver Tissue Lysate Catalog No: 1464



Rat liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat liver tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Kidney Tissue Lysate Catalog No: 1465



Rat kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Spleen Tissue Lysate Catalog No: 1466



Rat spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM







Application:

Rat spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Skeletal Muscle Tissue Lysate Catalog No: 1467



Rat skeletal muscle tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Rat skeletal muscle tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:





Toll Free: 888-513-9525 Tel: 858-513-2638 Fax: 858-513-2692


Rat Small Intestine Tissue Lysate Catalog No: 1468



Rat small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer







Application:

Rat small Intestine tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg


Storage:




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Tel: 858.513.2638 Fax: 858.513.2692

Toll Free: 888.513.9525 www.prosci-inc.com

Protocols

WESTERN BLOT PROCEDURE 1) Load 20 to 25 microgram of whole cell lysate per lane in an SDS-PAGE mini gel. 2) Run at 20 mA per gel untill the dye front is close to the bottom. 3) Transfer the proteins to a nitrocellulose membrane (S&S NCTM) at 250 mA in

transfer buffer for 1-4 h, depending on the size of the target protein. 4) Incubate the blot with blocking buffer (5% non-fat dry milk in TBS) overnight at 4oC

or 2 h at room temperature (RT). 5) Incubate the blot with primary antibody (diluted 1:250 to 1:1000 in blocking buffer)

for 1 h in blocking buffer at RT. 6) Wash the blot 3 x 10 min in washing buffer (TBS containing 0.1% Tween 20) with

shaking. 7) Incubate blot with anti-rabbit IgG-HRP conjugate (Sigma) (diluted 1:10,000 -

1:2,000 in blocking buffer) for 1 h in blocking buffer at RT. 8) Wash 3 x 10 min in washing buffer with shaking. 9) Drain washing buffer, add ECL solution (Amersham) and develop for 1 min. 10) Expose to X-ray film for 1 to 30 min.

TBS: • 125 mM NaCl • 25 mM Tris pH 8.0

SDS/Running Buffer: • 25 mM Tris

• 192 mM Glycine • 1% SDS

Transfer Buffer: • 20 mM Tris pH 8.0

• 150 mM Glycine • 20% methanol

CELL LYSATE PROCEDURE 1) Wash the cell pellets with ice-cold PBS. 2) Add 1 ml of RIPA buffer per 108 cells and incubate on ice for 20 min, vortex 2-3

times, briefly. 3) Aliquat cell lysate into 1.5 ml microfuge tubes and centrifuge at 13,000 rpm, 4oL for

5 min in an Eppendorf microfuge. 4) Transfer the supernatant into clean tubes. Measure the protein concentration with

BioRad protein assay. 5) Adjust the concentration to 5 mg/ml with RIPA lysis buffer. 6) Add an equal volume of 2 x SDS sample buffer into the cell lysate. Boil for 5 min. 7) Store at -20oC for daily use or -80oC for long term storage.

RIPA Buffer: • 50 mM Tris-HCl pH 7.4 • 150 mM NaCl • 1 mM PMSF • 1 mM EDTA • 5 µg/ml Aprotinin • 5 µg/ml Leupeptin • 1% Triton -100 • 1% Sodium deoxycholate • 0.1% SDS

2 x SDS/Sample buffer: • 100 mM Tris

• 25% glycerol • 2% SDS • 0.01% bromphenol blue • Adjust to pH 6.8 • Add 2-ME to 10%

Antibody Product list - ProSci Incorporated · Product Name Cat. No. Species Application...

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