Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal &...
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![Page 1: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/1.jpg)
Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland
Speakers: Neil Agarwal & Robert Vocke III
University of Maryland, College Park
![Page 2: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/2.jpg)
Establishing a Baseline
MDE
Clean Water Act
Antibiotics
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Site Overview
Non-tidal, mitigation sites 3 plots per site
![Page 4: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/4.jpg)
Data Collection
Vegetation
Soils
Land Use
Water Quality
Antibiotic Resistance
![Page 5: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/5.jpg)
Our focus today…
Water quality data
Why Antibiotic Resistance?
The Concept of Measuring Resistance
Preliminary Results
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Water Quality Results
Nitrate
0.050
1.246
0.000
0.200
0.400
0.600
0.800
1.000
1.200
1.400
1.600
1.800
Surface Subsurface
Nit
rate
(m
g/L
)
![Page 7: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/7.jpg)
Water Quality (continued)
Ammonia
0.924
0.221
0.000
0.200
0.400
0.600
0.800
1.000
1.200
Surface Subsurface
Am
mo
nia
(m
g/L
)
![Page 8: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/8.jpg)
Water Quality (continued)
Total Nitrogen
4.4904.267
0.000
1.000
2.000
3.000
4.000
5.000
6.000
Surface Subsurface
To
tal
N (
mg
/L)
![Page 9: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/9.jpg)
Water Quality (continued)
Total Phosphorus
2.300
0.487
0.000
0.500
1.000
1.500
2.000
2.500
3.000
3.500
4.000
Surface Subsurface
To
tal
P (
mg
/L)
![Page 10: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/10.jpg)
Why look at antibiotic resistance? The pharmaceutical problem
Antibiotics and resistance Testing for antibiotic resistance is cost
effective Questions to be asked:
Prevalence and temporal changes Correlation to other aspects of health Resistance ‘sinks’ Nutrient Cycling
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Disk Diffusion: The Concept
Image courtesy of http://biology.clc.uc.edu/fankhauser/Labs/Microbiology
Image courtesy of http://jyi.org/articleimages/555/
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Disk Diffusion: The Concept
Image courtesy of http://www.wings-china.com/showinfo.asp?typeid=24&xxid=554&Language=En
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Fleming’s Discovery
Image courtesy of http://www.accessexcellence.org/AE/AEC/CC/s5.html
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Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
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Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
![Page 16: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/16.jpg)
Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
![Page 17: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/17.jpg)
Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
![Page 18: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/18.jpg)
Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
![Page 19: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/19.jpg)
Disk Diffusion: The Procedure
Image courtesy of http://jyi.org/articleimages/555/
![Page 20: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/20.jpg)
Our specific procedure
Collected surface samples Serial dilutions Culture on ColiBlue
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ColiBlue media
Red = Coliforms Blue = E. Coli
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Our specific procedure
Collected surface samples Serial dilutions Culture on ColiBlue Isolate a single E. coli colony Disk diffusion using 5 antibiotics:
Ampicillin Erythromycin Tetracycline Sulfisoxazole Ciprofloxacin Negative Control: Vancomycin
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Converting Inhibition Zones
CLSI standard Example: Ampicillin with 7mm
zone of inhibition
<13 mm = Resistant 14-16 mm = Intermediate >17 mm = Susceptible
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Converting Inhibition Zones
CLSI standard Example: Ampicillin with 7mm
zone of inhibition
<13 mm = Resistant 14-16 mm = Intermediate >17 mm = Susceptible
![Page 25: Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland Speakers: Neil Agarwal & Robert Vocke III University of Maryland, College Park.](https://reader035.fdocuments.us/reader035/viewer/2022081506/56649d1a5503460f949efd1a/html5/thumbnails/25.jpg)
Results of Testing
Ampicillin Tetracycline Ciprofloxacin Sulfisoxazole
Susceptible 1 2 32 11
Intermediate 5 13 2 12
Resistant 27 18 0 11
Total 33 33 34 34
% Resistant 82% 55% 0% 32%
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What’s next?
Find correlations to health Test upstream & downstream Effect on nutrient
cycling
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Antibiotic Resistant Escherichia Coli in Non-Tidal Wetlands of Maryland
Speakers: Neil Agarwal & Robert Vocke III
University of Maryland, College Park