Annual Meeting Sheffield – 22nd March, 2019 The Circle Conference venue, http://www.thecirclesheffield.org.uk/

Final timings are still subject to minor changes https://www.cilianetwork.org.uk/events/4595/2019-uk-cilia-network-meeting The organisers of the meeting would like to thank the following companies for their generous support for the meeting.

09.30-10.15 Registration and Coffee 10.15-10.45 Welcome - Gwen Reilly and Colin Bingle, In remembrance of Jarema Maliki Colin Johnson - for the UK cilia community Freek van Eeden – for the Bateson/BMS Update on the UK Cilia Network Martin Knight. . Session 1 Chair: Dominic Norris 10.45-11.05 Robert Hirst1, C Watson2, A Rutman1, G Williams1, P Chetciti2, E Sheridan, C O’Callaghan1,3. 1Centre for PCD Diagnosis and Research, Department of Respiratory Sciences, University of Leicester, 2School of Medicine, University of Leeds, 3Respiratory, Critical Care & Anaesthesia, Institute of Child Health, UCL & Great Ormond Street Children’s Hospital Do mutations in LRRC56 cause human motile ciliopathies? 11.05-11.25 Hermes Gadelha1, P Hernández-Herrera2, F Montoya2, A Darszon3, G Corkidi2. 1Department of Mathematics, University of York, 2 Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Mexico. 3Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Mexico. How does the sperm flagellum beat in 4D? 11.25-11.45 Basudha Basu1, CA.Johnson1, J. Bond1, R. Taylor1, K Khan 1,2, M McKibbin2, C Inglehearn1, J. Ladbury3. 1Leeds Institute of Medical Research, University of Leeds, 2Dept. of Ophthalmology, Leeds Teaching Hospitals NHS Trust, 3Astbury Centre, Faculty of Biological Sciences, University of Leeds. Developing a stem cell facility for the molecular modelling of genetic variants in inherited retinal dystrophies 11.45- 12.05 Asma Boukhalfa, N Dupont, P Codogno, E Morel.. INSERM U1151-CNRS UMR8253, Descartes-Sorbonne Paris Cité University, Paris, France.

Non-canonical role of ATG16L1 in primary cilium trafficking and functions 12.05-12.25 John Vakonakis1, A Kantsadi1, G Hatzopoulos2, P Gönczy2. 1University of Oxford, 2Swiss Federal Institute of Technology (EPFL), Switzerland. Structural origins of cartwheel self-assembly by the centriolar protein SAS-6 12.25-13.25 Lunch & poster viewing (Prize - £100 from Portland Press/ Biochemical Society Transactions. Judging will be by popular vote with all attendees to indicate their preference by the tea break). Session 2 Chair: Pleasantine Mill 13.25-14.00. Ronald Roepman, Radboud UMC, The Netherlands Social networking to deconvolute cilium (dys-) function 14.00-14.15 Introducing the Nanoimager - ONI Bio presentation 14.15-14.35 Ursala McClurg1,4, S Sandhu1, K Szymanska5, JE Hunter2 CL Wilson3, ND Perkins2, N

Hunter1, OR Davies2. 1Howard Hughes Medical Institute University of California Davis, USA.

2Institute for Cell and Molecular Biosciences, Newcastle University, 3Institute of Cellular Medicine, Newcastle University. 4Institute for Integrative Biology, University of Liverpool. 5Leeds Institute of Medical Research, University of Leeds.

A novel centrosomal function of a meiotic protein, TEX12 in cancer 14.35-14.55 Alice Lake1, G Grant1, C Galloni1, J Bond1, E Morrison1[1], M Olson2, G Marvia1, CA

Johnson1. 1Leeds Institute of Medical Research, University of Leeds, 2Ryerson

University, Toronto Actin remodelling, regulated by ROCK2, modulates

ciliogenesis 14.55-15.15 Patricia Yeyati1, MJ Ford2, MA Keighren1, IJ Jackson1, P Mill1, RL Mort3.

1MRC Human Genetics Unit, Edinburgh; 2Goodman Cancer Research Centre, McGill University, Canada; 3Division of Biomedical and Life Sciences, , Lancaster University, Lancaster

A fluorescent tri-cistronic biosensor of cilia and cell cycle in live cells and mice. 15.15-15.35 Rosie Little1,2, RV Walker1,2,3, JL Keynton1, DP Norris1. 1Cilia, Development & Disease Group, MRC Harwell Institute, 2Department of Physiology, Anatomy & Genetics, University of Oxford, 3University of Maryland, USA (current) Examining vesicles and Fgf signalling in the mouse node. 15.35-15.55. Tea Session 3 Chair: Hannah Mitchison 15.55-16.30 Albert Ong Infection, Immunity and Cardiovascular Disease, University of Sheffield. New treatments for ADPKD 16.30-16.50 Melis Dalbay1, RA Hirst2, H Mitchison1, C O’Callaghan1, SL Hart1. 1University College London, Institute of Child Health. 2University of

Leicester. A cell line model for primary ciliary dyskinesia 16.50-17.10 Isabella Collins1, Š Bálint2, CR Coveney1, K Yamamoto3, F Bangs4, L Troeberg1, AKT Wann1. 1Arthritis Research UK Centre for Osteoarthritis Pathogenesis, Kennedy Institute of Rheumatology, University of Oxford, 2Kennedy Institute of Rheumatology, University of Oxford, 3Institute of Ageing and Chronic Disease, University of Liverpool, 4CRUK/MRC Institute for Radiation Oncology, University of Oxford The primary cilium in the regulation of cartilage matrix remodelling: a hotspot for endocytic clearance of proteases? 17.10-17.30 Victor Hernandez-Hernandez1,4 A Abarrategi1, J Jeyabalan1, MM Munye1, SC de Castro1, M Chawda1, R

Ruiz1, SN Waddington2, AA Rahim3, PL Beales1. 1Great Ormond Street Institute of Child Health, UCL, 2Institute of Women

Health, Maternal and Fetal Medicine, UCL, 3UCL School of Pharmacy, 4Department of Life Sciences, Brunel University London.

Gene therapy rescues CNS defects and obesity in ciliopathies

17.30 Meeting wrap up – move to the Devonshire Cat

Posterpresentations.1: Theroleofprimaryciliaincontrollingstemcellsinthedeveloping cerebralcortex.KerstinHasenpusch-Theil1,ChristineLaclef2,Eamon Fitzgerald1,MattColligan1,SylvieSchneider-Maunoury2,ThomasTheil1 1UniversityofEdinburgh,CentreforDiscoveryBrainSciences, Edinburgh. 2UniversitéPierreetMarieCurie,DevelopmentalBiologyLaboratory,75252 ParisCedex05,France2: PrimaryciliaelongationenhancesmechanosensitivityLiamABoyle1,Ingvar Kiricenko1,DamienLacroix2,GwendolenC.Reilly1 1INSIGNEOInstituteforinsilicoMedicine,DepartmentofMaterials ScienceandEngineering,UniversityofSheffield.2Departmentof MechanicalEngineeringUniversityofSheffield,UnitedKingdom3: InvestigatingthemechanismsofciliarylocalisationinPKD2function Laura.M.Dyer,V.Sreekumar,C.T.Esapa,R.V.Walker,D.P.Norris. MRCHarwellInstitute4: SurveyofciliarymotilitygenesinDrosophilaspermandciliated mechanosensoryneuronsrevealssubstantialcell-type-specificdifferences motileciliaandspermflagella.Petra.zurLage,F.G.Newton,A.P.Jarman. UniversityofEdinburgh,SBMS(CentreforDiscoveryBrainSciences)5: Twitchy,theDrosophilaFBF1/dyf-19orthologueisrequiredfor coordinatedlocomotionandciliaryfunction.SHodge,JBirkhead, LindsayKMacDougall. UniversityofManchester6: Mcidasmutantmicerevealatwo-stepprocessforthespecificationand differentiationofmulticiliatedcellsinmammals.HLu1,Priyanka Anujan1,2,FZhou1,YZhang1,YLChong1,CDBingle2,S Roy1. 1AgencyofScienceTechnologyandResearchSingapore.2Departmentof Infection,ImmunityandCardiovascularDisease,UniversityofSheffield7: Transitionsinsynchronizationstatesofmodelciliathroughbasal- connectioncoupling.Y.Liu,R.Claydon,D.R.Brumley,MarcoPolin. UniversityofMelbourne,UniversityofWarwick8: Ciliaryregulationofpolycystinsignallinginchondrocytes.Clare.L. Thompson,H.M.Mitchison,P.L.Beales,M.Ramachandran,J.P.Chapple, M.M.Knight [1]InstituteofBioengineering,QueenMaryUniversityofLondon, London,UK.[2]GeneticsandGenomicMedicine,UCLInstituteofChild Health,London,UK.[3]WilliamHarveyResearchInstitute,Bartsandthe

LondonSchoolofMedicine,QueenMaryUniversityofLondon,London, UK9: UsingCRISPRtechnologytoendogenouslytagretinaldiseasegene CEP290withEGFPininducedpluripotentstemcells.Rowan.D.Taylor1 ,B.Basu1,C.A.Johnson1,J.Bond1,K.Khan1,2,MartinMcKibbin2,Chris Inglehearn1,J.Ladbury3. 1LeedsInstituteofMedicalResearch,SchoolofMedicine,UniversityofLeeds, 2Dept.ofOphthalmology,LeedsTeachingHospitalsNHSTrust,3Astbury Centre,FacultyofBiologicalSciences,UniversityofLeeds