Anatomy & Neurobiology ANA 812 Lab Session 7 - 2005 Muscle Tissue Katherine Freedman Jamie Lewis...

Anatomy & Neurobiology

ANA 812 Lab Session 7 - 2005

Muscle Tissue

Katherine FreedmanJamie Lewis

Jennifer MooreAndrea ThomasAndrew Usery


Slide #29 Skeletal Muscle – Human (H&E) at 4X

• Cross section

• Longitudinal section



• Perimysium• Endomysium• Peripheral Nuclei• Fascicle• Epimysium

4x



• Endomysium

• Perimysium• Nuclei• Fascicle• A, I, & Z

bands


Slide #73 Infant Tongue – Human (H&E) 4X


Slide #73 Infant Tongue – Human (H&E) 40X

• Fibers oriented at right angles to each other


Slide #20 Vertebra – Dog (H&E) 4X

• Muscle Spindle


Slide #20 Vertebra – Dog (H&E) 40X

• Skeletal Muscle

• Intrafusal Fibers

• Fluid Filled Space

• External Capsule (Connective Tissue)


Slide #30: Cardiac muscle – human (H&E)

4x 10x

Blood vessel


Slide #30:Cardiac muscle – human (H&E)

Intercalated discs

Bi-nucleated cell

Mono-nucleated cell

Striations

Branching cells

40x


Slide #32:Cardiac muscle – rat (H&E)

4x 10x



Blood vessel

Intercalated disc

40x



Branching cell

Striations

Bi-nucleated cell

Mono-nucleated cell

40x


Slide #37 Heart, Right Atrium - Human (H&E) 10x


• Cross section




• Nuclei: one per cell and centrally located

• Intercalated discs



• Lipofuscin granules


Slide #38 Heart- Sheep (Masson’s Tri-Chrome) 4x

• Cross section




• Cross section


• Intercalated discs



• Purkinje fibers– Pale staining in center of

cytoplasm– Peripherally located

myofibrils– Round nuclei


Slide #93 Colon-Monkey (H& E) 4X

•Smooth Muscle Cross-Section



•Smooth Muscle Cross-Section•Muscularis Externa

•Inner Layer (longitudinal)•Outer Layer (Cross-section)



•Cells packed closely together•No striations•Minimal Intracellular Space•Centrally Located, Ovoid Nuclei•Muscularis Externa

•Inner Layer (longitudinal)•Outer Layer (Cross-section)


Slide #87 Gastroduodenal Junction-Human (PAS &

Hematoxylin) 2X

•External Lamina

•Smooth Muscle Cells



Hematoxylin) 4X•Smooth Muscle Cells•External Lamina

•Glycoasminoglycans•Reticular Fibers



Hematoxylin) 10X

•External Lamina

•Smooth Muscle Cells



Hematoxylin) 40X


Similarities and Difference in Muscle Types (H & E) 4x

• Long Fibers Lack Branches

• Discernable Fiber Bundles

• Striations Lend Banded Appearance

• Fibers Branch and Anastomose

• Striations and Intercalated Discs are Indiscernible at Low Power

•Less Uniform Structure

•Lack Striations And Discs

•Tightly Packed Fibers

Skeletal Muscle Cardiac Muscle Smooth Muscle


Similarities and Differences In Muscle Types (H & E) 40X

• Discernable A & I Bands

• Multinucleated Fibers

•Long Fibers that Do Not Branch or Anastomose

•Striations Apparent, But Less Defined

•Mono- or Bi- Nucleated Cells

•Fibers Branch and Anastomose with less linear structure

• Fibers Lack Striation

• Single, Centrally Located Nucleus

• Short, Spindle Shaped Cells that Do Not Branch

Skeletal Muscle Cardiac Muscle Smooth Muscle

Anatomy & Neurobiology ANA 812 Lab Session 7 - 2005 Muscle Tissue Katherine Freedman Jamie Lewis...

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