Analysis of Triazine Pesticides Using a Core Enhanced ...
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Analysis of Triazine Pesticides Using a Core Enhanced Technology Accucore HPLC Column Anila Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Abstract This application note demonstrates the use of the Thermo Scientific Accucore C18 column, for the fast HPLC analysis of triazine herbicide and pesticides without losing resolution, all at low column back pressure. Introduction Triazines are widely used in weed control in agricultural and non-agricultural practices and work by inhibiting the photosynthetic pathways. If ingested by humans or animals they can prove to be poisonous leading to nausea, weight loss and weakness. Fast HPLC analysis of selected triazines was achieved using an Accucore™ C18 column. Accucore™ HPLC columns use Core Enhanced Technology to facilitate fast and high efficiency separations. The 2.6 μm diameter particles are not totally porous, but rather have a solid core and a porous outer layer. The optimised phase bonding creates a series of high coverage, robust phases. The carbon loading of Accucore C18 provides high retention of non-polar analytes via a predominantly hydrophobic interaction mechanism, enabling the separation of a broad range of analytes.The tightly controlled 2.6 μm diameter of Accucore particles results in much lower backpressures than typically seen with sub-2 μm materials. Results Figure 1 shows the chromatogram of the triazine herbicides and pesticides separated on an Accucore C18 2.6 μm 100 x 2.1 mm column under 3.0 minutes. The analysis was performed using fast HPLC demonstrating this column can provide good baseline resolution of triazines at low backpressure. Conclusions Separation of triazines was successfully achieved on an Accucore C18 column at low system backpressure. The column therefore provides an excellent choice for the fast analysis of these compounds, giving excellent retention time reproducibility. Key Words • Accucore C18 • Superficially porous • Core Enhanced Technology • Fused core • Triazines • Herbicides • Pesticides Application Note: ANCCSCETPEST Sample Preparation A 1000 μg/mL solution of triazine herbicides and pesticides was prepared in acetonitrile; this solution was then diluted in water to the required concentration required for analysis. (refer to Table 1) Thermo Scientific Column Part Number Accucore C18 2.6 μm 100 x 2.1 mm 17126-102130 Measured back pressure: 298 bar Thermo Scientific Accela HPLC/UHPLC Column temperature 40 °C Injection volume 2 μL Flow rate 0.60 mL/min PDA/UV detection 280 nm Mobile Phase Mobile phase A Water Mobile phase B Acetonitrile Gradient: Time/min A% B% 0.00 65 35 1.00 65 35 2.50 30 70 2.60 65 35 Consumables Part Number Fisher Scientific HPLC grade water W/0106/17 Fisher Scientific HPLC grade acetonitrile A/0626/17 Liquid handling hardware: FinnPippette (100-1000μL) 642090 NSC Mass Spec Certified 2mL clear vial with MSCERT4000-34W PTFE silicone cap
Transcript of Analysis of Triazine Pesticides Using a Core Enhanced ...
Analysis of Triazine Pesticides Using a CoreEnhanced Technology Accucore HPLC Column Anila Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK
Abstract
This application note demonstrates the use of the ThermoScientific Accucore C18 column, for the fast HPLC analysisof triazine herbicide and pesticides without losingresolution, all at low column back pressure.
Introduction
Triazines are widely used in weed control in agriculturaland non-agricultural practices and work by inhibiting thephotosynthetic pathways. If ingested by humans oranimals they can prove to be poisonous leading to nausea,weight loss and weakness.
Fast HPLC analysis of selected triazines was achievedusing an Accucore™ C18 column. Accucore™ HPLCcolumns use Core Enhanced Technology to facilitate fastand high efficiency separations. The 2.6 µm diameterparticles are not totally porous, but rather have a solidcore and a porous outer layer. The optimised phasebonding creates a series of high coverage, robust phases.The carbon loading of Accucore C18 provides highretention of non-polar analytes via a predominantlyhydrophobic interaction mechanism, enabling theseparation of a broad range of analytes.The tightlycontrolled 2.6 µm diameter of Accucore particles resultsin much lower backpressures than typically seen withsub-2 µm materials.
Results
Figure 1 shows the chromatogram of the triazineherbicides and pesticides separated on an Accucore C182.6 µm 100 x 2.1 mm column under 3.0 minutes. Theanalysis was performed using fast HPLC demonstratingthis column can provide good baseline resolution oftriazines at low backpressure.
Conclusions
Separation of triazines was successfully achieved on anAccucore C18 column at low system backpressure. Thecolumn therefore provides an excellent choice for the fastanalysis of these compounds, giving excellent retentiontime reproducibility.
Key Words
• Accucore C18
• Superficially porous
• Core Enhanced Technology
• Fused core
• Triazines
• Herbicides
• Pesticides
Application Note:ANCCSCETPEST
Sample Preparation
A 1000 µg/mL solution of triazine herbicides and pesticides was prepared inacetonitrile; this solution was then diluted in water to the requiredconcentration required for analysis. (refer to Table 1)
Thermo Scientific Column Part Number
Accucore C18 2.6 µm 100 x 2.1 mm 17126-102130Measured back pressure: 298 bar
Thermo Scientific Accela HPLC/UHPLC
Column temperature 40 °C Injection volume 2 µLFlow rate 0.60 mL/minPDA/UV detection 280 nm
Mobile Phase
Mobile phase A WaterMobile phase B AcetonitrileGradient:Time/min A% B%0.00 65 351.00 65 352.50 30 702.60 65 35
Consumables Part NumberFisher Scientific HPLC grade water W/0106/17Fisher Scientific HPLC grade acetonitrile A/0626/17Liquid handling hardware: FinnPippette (100-1000µL) 642090NSC Mass Spec Certified 2mL clear vial with MSCERT4000-34WPTFE silicone cap
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ANCCSCETPEST 0611
Figure 1: Chromatogram of triazines compounds mixture separated on an Accucore C18 2.6 µm 100 x 2.1 mm column
Table 1: The analysis of triazines compounds on an Accucore C18 2.6 µm 100 x 2.1 mm column
Time (Minutes)0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5
mAU
0
10
20
30
40
50
60
70
80
90
100
1
2
3
4
5
6
Compounds tr/min tr/min %RSD (n=6)
1 Simazine 0.78 0.12
2 Simetryn 1.17 0.18
3 Atrazine 1.27 0.17
4 Ametryn 1.98 0.13
5 Propazine 2.07 0.11
6 Prometryn 2.55 0.12
