Analysis of Physiological Characteristics and Gene Expression Associated with Different Light...
-
Upload
prudence-jennings -
Category
Documents
-
view
220 -
download
0
Transcript of Analysis of Physiological Characteristics and Gene Expression Associated with Different Light...
![Page 1: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/1.jpg)
Analysis of Physiological Characteristics and Gene Expression Associated with Different
Light Conditions in Brachypodium distachyon
By Jared Streich,Research lead by
Sam Fox and Dr. Todd Mockler
![Page 2: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/2.jpg)
Introduction
• Light sensitive proteins called phytochromes and cryptochromes regulate and or trigger certain biochemical and cellular processes.
![Page 3: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/3.jpg)
Phytochromes and Cryptochromes
• What is a phytochrome and cryptochrome?
![Page 4: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/4.jpg)
Brachypodium distachyon
• Grass model organism for monocot plants
• Ideal for research
• Accession Bd21 used
![Page 5: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/5.jpg)
Arabidopsis thaliana vs. Brachypodium distachyon
• Arabidopsis is limited in modeling monocots plants
• Brachypodium is a monocot
![Page 6: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/6.jpg)
Question• What are the affects of light quality on
Brachypodium distachyon growth, development and regulation of gene expression?
![Page 7: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/7.jpg)
Goals
• Identified physiological differences
• Create lists of up and down regulated genes
• Mapped metabolic pathways
• Identified phytochrome and cryptochrome genes
![Page 8: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/8.jpg)
Hypothesis
• Plants grown in certain colors of light will have differences in physiology and gene expression.
![Page 9: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/9.jpg)
Experiment Composition
–Analyze gene expression characteristics
–Analyze growth and development
–Conclude relations of both
![Page 10: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/10.jpg)
Gene Expression
Red
Blue
White + Far-red
White
Vs.
Dark (Control)
![Page 11: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/11.jpg)
Gene Expression
![Page 12: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/12.jpg)
Gene Data Analysis• Microarray technology used to analyze cDNA
![Page 13: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/13.jpg)
Gene Data Analysis
• Create clusters of up and down regulated genes via p-value
![Page 14: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/14.jpg)
Conclusion: Gene Count Analysis
• Shared genes in red and white light
• Shared genes in blue light and white light
• Most down regulated genes in white light
• Most up regulated genes in blue light
![Page 15: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/15.jpg)
Gene Data Analysis
![Page 16: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/16.jpg)
Conclusion: Genes Isolated as Likely Candidates for Phytochrome and Cryptochromes in BD21
• Phytochrome A Bradi1g10520.1
• Phytochrome B Bradi1g64360.1
• Phytochrome C Bradi1g08400.1
• Cryptochrome A Cry1a Bradi3g46590.1
• Cryptochrome B Cry1b Bradi5g11990.1
• Cryptochrome C Cry2 Bradi3g49204.1
![Page 17: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/17.jpg)
Physical Measurements
Tissues length–Root length
–Above ground tissue
![Page 18: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/18.jpg)
Conclusion: Physiological
• Red: Most overall growth
• White: Most root growth
• White + far-red: Longest leaves
• Blue: slowed growth in all tissue types.
![Page 19: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/19.jpg)
Biomass and Physiological Growth
![Page 20: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/20.jpg)
Flowering Time• All conditions flowered within all standard
deviations
![Page 21: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/21.jpg)
Summary
• Possibly identified phytochrome and cryptochrome genes
• Biomass varies by light condition
• Physiological growth varies by light condition
• Flowering time is not effected by light conditions
![Page 22: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/22.jpg)
Future Directions:
• Continue with gene expression analysis
• Isolate and over express genes of interest
• Transgenic plants of other genus with isolated genes
• Affects of adding far-red on white light condition
• Research flowering time regulation
![Page 23: Analysis of Physiological Characteristics and Gene Expression Associated with Different Light Conditions in Brachypodium distachyon By Jared Streich, Research.](https://reader030.fdocuments.us/reader030/viewer/2022033100/56649d035503460f949d6428/html5/thumbnails/23.jpg)
Acknowledgements
• Ernest and Pauline Jaworski• Howard Hughes Medical Institute• Dr. Kevin Ahern• Dr. Todd Mockler• Sam Fox• Erik Rowley• Jessica Murray• Dr. Serge Filichkin