Analysis of Phenotypic Data Using PM Software

File ManagementParametric Analysis

v. 1.2.02

Michael Ziman, Ph.D.

Director, PM Services

Overview

• File Management Version/Replicate Analysis– Organize OmniLog data– View replicate and average graphs– Export raw data replicates

• Parametric Version/Pairwise Comparison– View replicate and average graphs– Create Pairwise Comparison– Export average parameters

File Management/Kinetic Plot

Data Organization: by assay or filter

PM1.oka PM11.oka

PM2.oka PM12.oka

PM3.oka PM13.oka

PM4.oka PM14.oka

PM5.oka PM15.oka

PM6.oka PM16.oka

PM7.oka PM17.oka

PM8.oka PM18.oka

PM9.oka PM19.oka

PM10.oka PM20.oka

PM1.oka PM11.oka

PM2.oka PM12.oka

PM3.oka PM13.oka

PM4.oka PM14.oka

PM5.oka PM15.oka

PM6.oka PM16.oka

PM7.oka PM17.oka

PM8.oka PM18.oka

PM9.oka PM19.oka

PM10.oka PM20.oka

File Management

Reproducibility Analysis Flowchart

Average Replicates

Display and Analyze Reproducibility

Selected Files in Data List

Reproducibility Analysis

Export Kinetic Data

Raw Data Replicates

PM1.oka PM11.oka

PM2.oka PM12.oka

PM3.oka PM13.oka

PM4.oka PM14.oka

PM5.oka PM15.oka

PM6.oka PM16.oka

PM7.oka PM17.oka

PM8.oka PM18.oka

PM9.oka PM19.oka

PM10.oka PM20.oka

PM1.oka PM11.oka

PM2.oka PM12.oka

PM3.oka PM13.oka

PM4.oka PM14.oka

PM5.oka PM15.oka

PM6.oka PM16.oka

PM7.oka PM17.oka

PM8.oka PM18.oka

PM9.oka PM19.oka

PM10.oka PM20.oka

MUT.dlb

WT.dlb

File Management

Parametric Analysis

Pairwise

Comparison

Pairwise Comparison Flowchart

Parametric Version: Load Data List(s)

Calculate Parameters

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1 21 41 61 81 101 121 141 161 181

Time (Hour)

Om

niL

og

Sig

na

l

0

10

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100

Slo

pe

a-D-Glucose1st Derivative

Slope

Maximum Signal

Time to Maximum Slope

Lag

Average Height = sum of reads divided by number of reads

dXdY

TEST vs REFERENCE~2000 Kinetic Curves

Defects in Tryptophan Metabolism

Report

Export Parameters

Average Parameters or Paramter Difference Values