Inter. J. of Phytotherapy / Vol 4 / Issue 4 / 2014 / 154-161.

~ 154 ~

e - ISSN - 2249-7722

Print ISSN - 2249-7730

International Journal of Phytotherapy

www.phytotherapyjournal.com

ANALYSIS OF OIL COMPOSITION OF THE BENZENE EXTRACT

OF CORIANDRUM SATIVUM LINN FRUIT BY SOXHLATION AND

MACERATION TECHNIQUE

Padmaa M Paarakh1,*

1Department of Pharmacognosy, The Oxford College of Pharmacy, Bangalore 560 068, Karnataka, India.

INTRODUCTION

Coriander [Coriandrum sativum Linn.] an annual

of the Apiaceae family is one of valuable medicinal and

seasoning plant. This species comes from the

Mediterranean region and it is grown all over the world.

The coriander fruit and essential oil isolated from it are

used for medicinal purpose. It is used to treat menstrual

disorder, secondary infertility, ovaritis and cervicitis. It is

used to treat female diseases such as menoxenia,

ovulation type dysfunctional uterine bleeding [1]. It is

aphrodisiac to enhance sexual function and reproductive

capacity. It is used for treating leucorrhea; spermatorrhea.

Coriander fruit possess stimulant and carminative

properties [2]. Its oil is bactericidal and larvacidal [3]. It is

hypoglycemic and anti-inflammatory [4]. The fruits are

used as astringent, anthelmintic, emollient, stomachic,

antibilious, digestive, appetizer, constipating, diuretic,

antipyretic, refrigerant, tonic, expectorant, anodyne,

antidiabetic and dyspepsia [5]. It is

reported that coriander oil contains linalool and 20%

hydrocarbons which differ from the seed oil [6].

Therefore present aim is to determine the change in the oil

composition of the benzene extract of C.sativum fruits by

two different method of extraction Viz., Soxhlation and

maceration using GC-MS technique.

MATERIAL AND METHODS

Plant material and extraction procedure

Coriandrum sativum fruits were collected from

local market in Bangalore, Karnataka, India and it was

identified and authenticated by Botanist, Natural

Remedies Pvt Ltd., Bangalore. The fruits were dried in

shade and powdered coarsely. 350 g of powdered fruits

were macerated with petroleum ether followed by

benzene for 24 hrs and filtered and concentrated in Rota

evaporator and dried. Similarly 250g of fruits were

packed in the soxhlet apparatus and extracted with

Corresponding Author:- Padmaa M Paarakh Email: padmaparas@hotmail.com

ABSTRACT

Coriandrum sativum Linn [Family Apiceae] is a medicinal herb used in the treatment of various disease and

disorders. The present investigation was carried out to determine the change in the oil composition of the benzene

extract of C.sativum fruits by two different method of extraction Viz., Soxhlation and maceration using GC-MS

technique. The analysis revealed that by Soxlation method, a total of 155 compounds were obtained with major

constituents as Trichloromethane [2.39%], 2-Tridecenal(E) [2.18 %] and 1,2, benzene dicarboxylic acid, bis(2-methyl

propyl)ester [2.37%] whereas maceration method yielded 112 compounds with major constituents as 2-Tridecenal(E)

[1.33 %],1-Hexadecanol[1.40%], 1,2, benzene dicarboxylic acid, bis(2-methyl propyl)ester [1.22%]. The analysis

concludes that soxhlation method is better than maceration technique.

Key words: Coriandrum sativum, GC-MS analysis, Soxhlation, Maceration.

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petroleum ether followed by benzene for 18 hours. The

petroleum ether and benzene extracts were concentrated

by rotaevaporator and dried. The percentage yield of

petroleum ether and benzene extracts were found to be

4.35, 3.97 and 10.35, 2.36 % w/w respectively with

respect to air dried plant material.

Gas Chromatography-mass Spectrum Analysis [GC-

MS]

GC-Ms technique was used in this study to

identify the components present in the extract which was

carried out at Indian Institute of Science, Bangalore. GC-

MS analysis was performed using GC Thermo scientific,

Trace GC ultra and gas chromatograph interfaced to a

Mass spectrometer DSQII equipped with Zebron ZB 5 ms

capillary column[Length : 30 m, diameter:0.25 mm, film

thickness:0.25 m. For GC-MS detection, an electron

ionization energy system with ionization energy of 70eV

and Quadrapole as analyzer was used. Helium gas

[99.99%] was used as the carrier gas at a constant flow

rate of 1 ml/min and an injection volume of 1 l was

employed [split ratio: 10]. The oven temperature was

programmed from 40oC [isothermal for 2 min], with an

increase at the rate of 10oC/min to 300

oC for 5 min. Mass

spectra were taken at 70eV; a scan interval of 5 min with

scan range of 30-600 m/z. Toal GC running time was 60

min.The relative percentage amount of each component

was calculated by comparing its average peak area to the

total areas. The spectrum of unknown component was

compared with spectrum of the known compound stored

in the software libray [Xcaliber and AMDIS]. The name,

retention time and % area of the component of the test

material was ascertained with the data given in the

AMDIS library.

RESULT AND DISCUSSION

The Fig no 1 and 2 gives the GC-MS spectrum

of oil obtained from benzene extract of Coriandrum

sativum by soxhlation and maceration respectively. It can

be seen from Table No: 1 that total of 155 compounds

were obtained with major constituents as

Trichloromethane [2.39%] , 2-Tridecenal(E) [2.18 %] and

1,2, benzene dicarboxylic acid, bis(2-methyl propyl)ester

[2.37%] whereas maceration method yielded 112

compounds with major constituents as 2-Tridecenal(E)

[1.33 %],1-Hexadecanol[1.40%], 1,2, benzene

dicarboxylic acid, bis(2-methyl propyl)ester [1.22%].

The other major constituents by soxhlation method were

undecane [1.50%], 1-Hexadecan0l [0.70 %], menthol

[0.73 %], dodecanal 0.70%], phthalic acid, butyl 2-ethyl

butyl ester [1.07%], dibutyl phthalate [0.82 %] and 2, 3,

dihydroxy propyl elaidate [0.62%]. The other major

constituents by maceration method were undecane

[0.96%], 3,7,Octadiene-2,6,diol, 2,6-dimethyl [0.97%],

1,7, Octadiene-3,6,diol, 2,6,dimethyl [0.68%] and

phthalic acid, hept-4yl oisobutyl ester [1.22%].

Table 1. Chemical composition of oil obtained from benzene extract of Coriandrum sativum by soxhlation

Sl.No. Name of the compound RT Amount

1 Trichloromethane 3.4941 2.39%

2 Carbon Tetrachloride 3.6305 0.45%

3 Methane, dichloronitro- 3.6524 0.25%

4 3-Buten-2-one, 3-methyl- 3.6851 0.07%

5 Pentanal 3.8109 0.06%

6 3-Penten-2-one, 4-methyl- 4.0168 0.01%

7 Cyclohexanone 4.0168 0.00%

8 Toluene 4.3474 0.01%

9 2,4,6-Trimethyl-1-nonene 4.4595 0.03%

10 Octane 4.6181 0.37%

11 Hexanal 4.64 0.44%

12 Cyclohexane, (1,1-dimethylethyl)- 4.6903 0.10%

13 Cyclotrisiloxane, hexamethyl- 4.7078 0.06%

14 Piperidine 4.8336 0.02%

15 1,3-Octadiene 4.894 0.03%

16 2,4-Dimethyl-1-heptene 5.0633 0.06%

17 Ethylbenzene 5.3466 0.00%

18 2-Cyclopenten-1-one, 3-methyl- 5.4789 0.01%

19 Heptane, 4-ethyl- 5.7906 0.07%

20 Heptanal 5.8464 0.05%

21 3-Carene 6.3014 0.00%

22 Cyclohexane, nitro- 6.4523 0.02%

23 2,4-Pentadien-1-ol, 3-ethyl-, (2Z)- 6.4523 0.04%

24 1-Hexanol, 2-ethyl- 6.6098 0.57%

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25 2-Heptenal, (Z)- 6.6098 0.57%

26 Benzaldehyde 6.7651 0.00%

27 Cyclotetrasiloxane, octamethyl- 6.8435 0.04%

28 Methyl vinyl ketone 6.8887 0.02%

29 1-Octen-3-ol 6.9667 0.08%

30 Cyclodecane 7.0758 0.06%

31 Benzene, 1,3,5-trimethyl- 7.1751 0.00%

32 Decane 7.1988 0.28%

33 Octanal 7.2813 0.07%

34 4-Ethylcyclohexanol 7.3981 0.04%

35 Benzene, 1,4-dichloro- 7.5029 0.00%

36 Benzene, 1,2-dichloro- 7.5029 0.00%

37 Decane, 4-methyl- 7.5242 0.00%

38 Benzene, tert-butyl- 7.6262 0.00%

39 Benzene, 1-methyl-3-(1-methylethyl)- 7.6262 0.00%

40 Cyclohexanone, 4-(1,1-dimethylethyl)- 7.6883 0.03%

41 3-Octen-2-one 7.8206 0.08%

42 1-Ethyl-2,2,6-trimethylcyclohexane 7.8359 0.04%

43 Indene 7.9573 0.00%

44 Dodecane 8.0077 0.02%

45 2,6-Dimethyldecane 8.0077 0.04%

46 2-Octenal, (E)- 8.1245 0.16%

47 1-Dodecanol 8.3405 0.10%

48 Cyclooctane, 1,4-dimethyl-, trans- 8.3405 0.10%

49 1-Octanol, 3,7-dimethyl- 8.4038 0.06%

50 Ethane, hexachloro- 8.4096 0.09%

51 1-Undecene 8.5736 0.19%

52 Undecane 8.7117 1.50%

53 Nonanal 8.7965 0.20%

54 Benzene, 1-ethyl-3,5-dimethyl- 9.0533 0.00%

55 1-Tridecene 9.6417 0.03%

56 2(3H)-Furanone, 5-hexyldihydro- 10.0549 0.02%

57 Naphthalene 10.1037 0.01%

58 3,7-Octadiene-2,6-diol, 2,6-dimethyl- 10.1496 0.30%

59 Butanoic acid, 2-hexenyl ester, (E)- 10.1627 0.18%

60 Silicon tetrafluoride 10.2091 0.03%

61 Decanal 10.2905 0.08%

62 2-Decenal, (E)- 11.1037 0.55%

63 5-Undecanone 11.1949 0.08%

64 Furan, 2-butyltetrahydro- 11.3188 0.09%

65 1,7-Octadiene-3,6-diol, 2,6-dimethyl- 11.3402 0.23%

66 Benzaldehyde, 4-propyl- 11.3553 0.20%

67 Undecanol-5 11.4668 0.12%

68 Propanal 11.5069 0.01%

69 Cyclohexasiloxane, dodecamethyl- 11.5304 0.04%

70 2,4-Decadienal, (E,E)- 11.5707 0.32%

71 2-Isopropyl-5-methyl-1-heptanol 11.5904 0.09%

72 Naphthalene, 2-methyl- 11.679 0.00%

73 Undecanal 11.7206 0.46%

74 Pentadecane 11.8682 0.05%

75 Bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl- 11.9077 0.50%

76 2,4-Decadienal, (E,E)- 11.9077 0.50%

77 2-Undecenal 12.5059 0.09%

78 1-Chloroundecane 12.5499 0.07%

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79 1-Undecanol 12.6164 0.42%

80 Formic acid, undecyl ester 12.6164 0.42%

81 Cyclododecane 12.7727 0.03%

82 Biphenyl 12.8034 0.00%

83 Tetradecane 12.9314 0.05%

84 1-Hexadecanol 13.0834 0.70%

85 Menthol 13.0834 0.73%

86 Dodecanal 13.0834 0.70%

87 Naphthalene, 1,8-dimethyl- 13.1702 0.00%

88 Naphthalene, 1,2-dimethyl- 13.1788 0.00%

89 1,4-Methanoazulene, decahydro-4,8,8-trimethyl-9-methylene-, [1S-1.alpha.,3a.be 13.2354 0.00%

90 Naphthalene, 1,5-dimethyl- 13.3962 0.00%

91 Cycloheptasiloxane, tetradecamethyl- 13.7167 0.03%

92 2-Dodecenal 13.8379 0.14%

93 2,6-Dimethyl-3,5,7-octatriene-2-ol, ,E,E- 14.0896 0.04%

94 Pentadecane 14.2034 0.08%

95 Tetradecane 14.2034 0.08%

96 Phenol, 2,4-bis(1,1-dimethylethyl)- 14.3565 0.05%

97 Propanoic acid, anhydride 14.3919 0.01%

98 Cyclopentane, 1-butyl-2-pentyl- 14.5238 0.03%

99 Octadecane 14.6135 0.05%

100 2-Butenedioic acid (Z)-, dibutyl ester 14.6354 0.08%

101 2-Tridecenal, (E)- 14.9012 0.16%

102 Pentadecane, 2,6,10,14-tetramethyl- 14.969 0.02%

103 2-Tridecenal, (E)- 15.1287 2.18%

104 Oxalic acid, cyclohexyl hexyl ester 15.2785 0.04%

105 Hexadecane 15.4265 0.10%

106 Benzene, (1-methylnonyl)- 15.4699 0.03%

107 Cyclooctasiloxane, hexadecamethyl- 15.6883 0.04%

108 Benzene, (1-butylheptyl)- 15.8145 0.03%

109 Benzene, (1-ethylnonyl)- 16.1995 0.03%

110 Z-12-Tetradecenal 16.3012 0.43%

111 Heptadecane 16.5681 0.11%

112 Hexadecane 16.5681 0.11%

113 Benzene, 1-ethyl-4-methyl- 16.6476 0.04%

114 Benzene, 1-ethyl-3-methyl- 16.6476 0.05%

115 Benzene, 1-ethyl-2-methyl- 16.6476 0.06%

116 Benzene, (1-butyloctyl)- 16.9316 0.03%

117 Sulfurous acid, hexyl octyl ester 17.0493 0.07%

118 9H-Fluorene, 4-methyl- 17.2305 0.00%

119 Heptadecyl heptafluorobutyrate 17.2735 0.03%

120 Benzene, (1-ethyldecyl)- 17.337 0.04%

121 Octadecane 17.6596 0.02%

122 Benzene, (1-methylundecyl)- 17.7664 0.05%

123 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester 18.3677 2.37%

124 Phthalic acid, butyl 2-ethylbutyl ester 18.8198 1.07%

125 Benzene, (1-methyldecyl)- 18.859 0.05%

126 Hexadecanoic acid, methyl ester 18.9648 0.02%

127 1-Dodecanol, 2-octyl- 19.1092 0.03%

128 Tetracosane 19.2394 0.03%

129 Dibutyl phthalate 19.3028 0.82%

130 Hexadecanoic acid, ethyl ester 19.6375 0.05%

131 Eicosane 19.7031 0.01%

132 9-Octadecenoic acid, methyl ester, (E)- 20.6733 0.06%

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133 Octadecanenitrile 20.7477 0.03%

134 Ethyl Oleate 21.2486 0.10%

135 Ethanone, 1-(2-hydroxyphenyl)- 21.5989 0.00%

136 2H-Pyran, 2-(2-heptadecynyloxy)tetrahydro- 22.2107 0.04%

137 Tricosane 22.4495 0.02%

138 9,12-Octadecadienoic acid (Z,Z)- 22.6294 0.15%

139 2H-Pyran, 2-(2-heptadecynyloxy)tetrahydro- 22.8075 0.38%

140 (R-(R*,R*))-4-(1,5-Dimethylhexyl)-1-cyclohexenecarboxylic acid 22.9711 0.44%

141 Triphenyl phosphate 23.3534 0.01%

142 2,3-Dihydroxypropyl elaidate 23.9122 0.62%

143 Pentacosane 24.1101 0.08%

144 Bis(2-ethylhexyl) phthalate 24.3688 0.05%

145 Diisooctyl phthalate 24.3688 0.08%

146 Heptacosane 24.8912 0.04%

147 Octacosane 25.6509 0.03%

148 Oleic acid, eicosyl ester 25.9861 0.07%

149 Triacontane 27.0754 0.09%

150 Tetratriacontane 27.0754 0.09%

151 Campesterol 30.2656 0.13%

152 Stigmasterol 30.5408 0.06%

Table 2. Chemical composition of oil obtained from benzene extract of Coriandrum sativum by Maceration

Sl.No. Name of the compound RT Amount

1 Trichloromethane 3.5406 0.41%

2 Carbon Tetrachloride 3.588 0.23%

3 Trichloromethane 3.6415 0.40%

4 4-Penten-2-one 3.6781 0.03%

5 Acetic anhydride 3.6976 0.02%

6 Methane, isocyanato- 3.7366 0.08%

7 Hexane, 2,2-dimethyl- 3.7366 0.08%

8 2-Pentanone, 3-methylene- 3.816 0.03%

9 Methane, bromodichloro- 3.8887 0.00%

10 Toluene 4.3367 0.01%

11 1-Butanol, 3-methyl- 4.5196 0.01%

12 Octane 4.5992 0.23%

13 Glutaraldehyde 4.6227 0.26%

14 Hexanal 4.6227 0.26%

15 Cyclotrisiloxane, hexamethyl- 4.7021 0.03%

16 o-Xylene 5.3389 0.00%

17 Heptanal 5.8373 0.04%

18 Cyclohexane, nitro- 6.4604 0.02%

19 1-Pentene, 3-ethyl-3-methyl- 6.4604 0.04%

20 1-Hexanol, 2-ethyl- 6.6112 0.29%

21 2-Heptenal, (Z)- 6.6112 0.29%

22 Ethane, pentachloro- 6.9651 0.01%

23 1-Octen-3-ol 6.9747 0.04%

24 Decane 7.2053 0.11%

25 Hexane, 2,4-dimethyl- 7.2053 0.12%

26 5-Isopropenyl-2-methylcyclopent-1-enecarboxaldehyde 7.3922 0.06%

27 Benzene, 1,2,3,5-tetramethyl- 7.6252 0.00%

28 Cyclohexane, 2-ethenyl-1,1-dimethyl-3-methylene- 7.6896 0.08%

29 5-Isopropenyl-2-methylcyclopent-1-enecarboxaldehyde 7.7188 0.03%

30 3,5-Octadien-2-ol 7.84 0.03%

31 2-Butenoic acid, 3-hexenyl ester, (E,Z)- 7.8473 0.04%

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32 Dodecane 8.0087 0.01%

33 Benzene, butyl- 8.0886 0.00%

34 2-Octenal, (E)- 8.1434 0.06%

35 2-Furanmethanol, 5-ethenyltetrahydro-,,5-trimethyl-, cis- 8.3618 0.03%

36 Ethane, hexachloro- 8.4231 0.05%

37 1-Undecanol 8.5729 0.10%

38 1-Tridecene 8.5729 0.10%

39 Undecane 8.7009 0.96%

40 3-Carene 8.7418 0.02%

41 Bicyclo[3.1.1]heptane, 6,6-dimethyl-2-methylene-, (1S)- 8.7418 0.05%

42 1,6-Octadien-3-ol, 3,7-dimethyl- 8.7418 0.05%

43 Nonanal 8.8008 0.13%

44 Borneol 9.9091 0.02%

45 Benzene, tert-butyl- 10.1027 0.01%

46 Naphthalene 10.1115 0.00%

47 3,7-Octadiene-2,6-diol, 2,6-dimethyl- 10.153 0.97%

48 Decanal 10.2971 0.08%

49 4,4-Dimethyl-1-hexene 10.6157 0.32%

50 2-Propenoic acid, 2-methyl-, (tetrahydro-2-furanyl)methyl ester 10.629 0.25%

51 1,3-Cyclohexadiene, 1-methyl-4-(1-methylethyl)- 10.6467 0.00%

52 2-Decenal, (E)- 11.1024 0.26%

53 5-Undecanone 11.1997 0.07%

54 2-Undecen-4-ol 11.3236 0.05%

55 1,7-Octadiene-3,6-diol, 2,6-dimethyl- 11.3489 0.68%

56 Undecanol-5 11.4659 0.04%

57 2-Undecanone 11.507 0.02%

58 2,4-Decadienal, (E,E)- 11.5713 0.19%

59 Camphor 11.5732 0.18%

60 Undecanal 11.7243 0.20%

61 Bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl- 11.9117 0.35%

62 2,4-Decadienal, (E,E)- 11.9117 0.35%

63 1-Tridecene 12.6397 0.13%

64 1-Undecanol 12.6417 0.19%

65 Tetradecane 12.9335 0.01%

66 Benzene, 1-ethyl-2,3-dimethyl- 13.0693 0.01%

67 Benzene, 1-methyl-3-(1-methylethyl)- 13.0693 0.00%

68 Menthol 13.089 0.31%

69 Dodecanal 13.089 0.35%

70 2-Dodecenal 13.8426 0.05%

71 Ethanone, 1-(1,4-dimethyl-3-cyclohexen-1-yl)- 14.0942 0.25%

72 Phenol, 2,4-bis(1,1-dimethylethyl)- 14.3646 0.01%

73 1,5,7-Octatrien-3-ol, 2,6-dimethyl- 14.9153 0.16%

74 2-Tridecenal, (E)- 15.1106 1.33%

75 1-Hexadecanol 15.1172 1.40%

76 Hexadecane 15.4202 0.01%

77 Tetradecanal 15.6074 0.04%

78 7-Tetradecenal, (Z)- 16.3065 0.27%

79 Heptadecane 16.5703 0.03%

80 9H-Fluorene, 4-methyl- 17.2441 0.00%

81 1-Tetradecanol 17.277 0.00%

82 Octadecane 17.6707 0.00%

83 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester 18.3434 1.22%

84 Phthalic acid, hept-4-yl isobutyl ester 18.3434 1.22%

85 Nonadecane 18.7201 0.01%

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86 Phthalic acid, 6-ethyl-3-octyl butyl ester 18.8214 0.49%

87 Hexadecanoic acid, methyl ester 18.969 0.01%

88 Dibutyl phthalate 19.3109 0.27%

89 Hexadecanoic acid, ethyl ester 19.641 0.02%

90 Pentadecane 19.7166 0.00%

91 Hexacosane 20.619 0.06%

92 Heneicosane 20.671 0.03%

93 cis-13-Octadecenoic acid, methyl ester 20.6768 0.04%

94 2H-Pyran-2-one, tetrahydro-6-octyl- 21.0687 0.05%

95 9-Octadecenoic acid (Z)-, methyl ester 21.2858 0.06%

96 Ethyl Oleate 21.2858 0.09%

97 Ethanone, 1-(2-hydroxyphenyl)- 21.6003 0.01%

98 Z-(13,14-Epoxy)tetradec-11-en-1-ol acetate 22.2146 0.04%

99 Tricosane 22.4562 0.07%

100 Tetracosane 22.4562 0.07%

101 5,8-Octadecadienoic acid, methyl ester 22.6367 0.08%

102 2H-Pyran, 2-(2-heptadecynyloxy)tetrahydro- 22.813 0.42%

103 Bis(2-ethylhexyl) phthalate 24.379 0.03%

104 Diisooctyl phthalate 24.379 0.06%

105 2-Pyrimidinamine, 4-(4-fluorophenyl)-6-(trifluoromethyl)- 24.6878 0.05%

106 Decanoic acid, octyl ester 24.6974 0.21%

107 Heptacosane 25.6454 0.01%

108 Tetracosane 27.0808 0.04%

109 Pentacosane 27.0808 0.04%

110 Heneicosane 27.085 0.10%

111 Campesterol 30.2706 0.20%

112 Stigmasterol 30.5302 0.16%

Fig 1. GC-Ms of oil obtained from Benzene extract of Coriandrum sativum by soxhlation

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Fig 2. GC-Ms of oil obtained from benzene extract of Coriandrum sativum by maceration

CONCLUSION

The result of this work suggests that the benzene

extract of Coriandrum sativum has number of

components which can act as a drug for various diseases.

The analysis revealed that by Soxlation method, a total of

155 compounds were obtained with major constituents as

Trichloromethane [2.39%] , 2-Tridecenal(E) [2.18 %] and

1,2, benzene dicarboxylic acid, bis(2-methyl propyl)ester

[2.37%] whereas maceration method yielded 112

compounds with major constituents as 2-Tridecenal(E)

[1.33%], 1-Hexadecanol [1.40%], 1,2,benzene

dicarboxylic acid, bis(2-methyl propyl)ester [1.22%].

The analysis concludes that soxhaltion method is better

than maceration technique. Further in future, these

components can be isolated and pharmacological activity

may be studied to ascertain the traditional use.

ACKNOWLEDGEMENT

The authors are grateful to Department of

Pharmacognosy, The Oxford College of Pharmacy,

Bangalore, for providing the facilities and the grant from

Research Promotion Scheme from All India Council for

Technical Education, New Delhi for carrying out the

entire experiment.

Ethical Issues

There is none to be applied.

Conflict of Interest

None to be declared.

REFERENCES

1. The Wealth of India, First supplement Series-Raw Material] 2:Cl-Cy, 2001, 205, National Institute of Science and Communication, Council of scientific and industrial Research, New Delhi, India.

2. Pullaih T, Encyclopedia of World Medicinal Plants, 2, 2006, 1641, Regency Publication, New Delhi, India. 3. Bently R, Trimen H, Medicinal Plants, 2, 2002, 729, Omsons Publications, New Delhi, India. 4. Khare PC, Indian Medicinal Plants, 2007, 174, Springer Science Publishers, NY, USA. 5. Paarakh PM. Coriandrum Sativum : A review, Pharmacologyonline News Letter, 3, 2009, 561-573, 6. Guenther E. The Essential Oil. Vol IV, Florida, USA, REK Publishing Company, 1950, 602-15.