Analysis of lytic proteins pinholin and Rz/Rz1
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Analysis of lytic proteins pinholin and Rz/Rz1 Sue Parks and Lawangin Khan
description
Analysis of lytic proteins pinholin and Rz/Rz1. Sue Parks and Lawangin Khan. Lytic Cassette. S. R. Rz. Rz1. Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins. Two Domains in the Pinholin Protein. Figure 1D: Phage S21 reading frame. - PowerPoint PPT Presentation
Transcript of Analysis of lytic proteins pinholin and Rz/Rz1
Analysis of lytic proteins pinholin and Rz/Rz1
Sue Parks and Lawangin Khan
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S R Rz
Rz1
Lytic Cassette
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Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins
Two Domains in the Pinholin Protein
Pang et al.(2010) Mutational Analysis of S21 pinholin. Molecular Biology, 76(1): 66-77.
Figure 1D: Phage S21 reading frame
Traditional Holin Model
TM1
TM2
TM3
TMD2TMD1
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Motifs TMD1 TMD2MXXM
Comparative analysis of lysis protein, Rz1 cleavage
sitesLawangin Khan
Localization? Why Cleave?
Cleave site
Cleavage site conserved? Determining cleavage site
• Determined by LipoP, (www.cbs.dtu.dk/services/LipoP/)
Comparative Analysis
Relative Consensus and Position
• Krupovic’s lipobox consensus: L(A/S)(G/A)C
