An Auditory Cortex Site Critical to Discrimination Learning
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Transcript of An Auditory Cortex Site Critical to Discrimination Learning
An Auditory Cortex Site Critical to Discrimination Learning
Adam Duvel and Mike Gabriel
Neural Pattern Analysis
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Auditory Cortical Lesions and LearningAuditory Cortical Lesions and Learning
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Auditory Cortex Lesions and TIAAuditory Cortex Lesions and TIA
Patterned Neurons Develop Patterned Neuronal Activity
Bruce Wheeler with
Deborah Leckband
Correlated Neuronal Activityfrom Neuronal Networks
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Are Neurons in Patterns More Active?
A. Patterned Networks Have Greater ActivityWithout Patterns: 1% ± 3% active electrodes
0.43 ± 0.35 Hz spike rateWith Patterns: 16% 12% active electrodes
2.13 ± 3.0 Hz spike rate
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B. Activity Increases with Cell Density
PatternedNeuron Cultures
Well Patterned
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Glia Develop More Rapidly On Patterns
Green – GFAP -- GliaRed –Neurofilament
8 days in culture
14 days in culture
22 days in culture
Evaluation of a Binaural FMV Beamforming Algorithm in
NoiseJeffery B. Larsen, Charissa R. Lansing,
Robert C. Bilger, Bruce Wheeler, Sandeep Phatak, Nandini Iyer, Mike Lockwood, William
O’Brien, Doug Jones, and Albert S. Feng
Research supported by NIH grant #DC04840
INTRODUCTION
A Frequency Banded Minimum Variance (FMV) algorithm has been developedPreliminary investigations with the FMV in simulation have been promisingEvaluation of the FMV in a multi-source environment is needed to demonstrate its effectivenessThe primary question of this study is: How much benefit from the FMV is obtained in noisy environments beyond that provided by directional microphones alone?
Setup of current preliminary investigation - Experiment #1
• Dependent variables– SRT in quiet and in noise with spondaic
words– SRT in quiet and in noise with HINT
sentences (Nilsson, Soli, & Sullivan, 1994)– Subject rated intelligibility of SIR passages
(Cox & McDaniel, 1989) at a -4 dB SNR
• 5 listeners w/ NH (20 - 40 years old) and 9 w/ SNHL (53 to 81 years old)
Setup #1
*Target Computer
Real-time system
Probe mic system
Cardioid mics
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* Reversed speech of four talkers speaking R-SPIN sentences at -40°, -20°, 20°, and 40° from target at 0°
CD player
Setup of current preliminary investigation - Experiment #2• Dependent variables
– SRT in quiet and in noise with HINT sentences (Nilsson, Soli, & Sullivan, 1994)
– Percent Correct of Keywords for CST Passages (Cox, Alexander, & Gilmore, 1987) at a 0 dB SNR
• 5 listeners w/ SNHL (65 - 80 years old) • Use of speech-shaped cafeteria noise
(Ricketts & Dhar, 1999) as jammer• Novel spacing and levels of jammers
CONCLUSIONS• FMV algorithm provides benefit across:• Competing signal types
– Speech shaped cafeteria noise and reversed speech• ESSENTIALLY EQUAL BENEFIT IN EACH
• Response Tasks– Intelligibility ratings - 50% higher for FMV than DM– Speech recognition - 36% improvement in WRS– Fixed and variable SRT measures
• 6 TO 8 dB OF SRT IMPROVEMENT OVER DIRECTIONAL MICS ALONE FOR NORMAL HEARING LISTENERS
• 8 TO 13 dB OF SRT IMPROVEMENT FOR LISTENERS WITH SNHL OVER DIRECTIONAL MICS ALONE DEPENDING UPON LOCATION OF JAMMERS
CONCLUSIONS (cont.)• Auditory Scenes
– Setup #1 - jammers equal at +20º and +40º (Thanksgiving table scene)
• VARIABLE SRT IMPROVEMENT OVER DIRECTIONAL MICS FOR LISTENERS WITH SNHL WAS 8 TO 11 dB
– Setup #2 - Closest jammer at +20º was more intense than other three jammers at -80º, -40º, and +60º (4th of July picnic scene)
• MEAN VARIABLE SRT IMPROVEMENT OVER DIRECTIONAL MICS FOR LISTENERS WITH SNHL WAS 13 dB (range of 8 to 19 dB across listeners)
FUTURE DIRECTIONS• Direct comparison of FMV with
commercially available hearing aids with technology for listening in noise
• Continued assessment of performance in different noise source configurations and reverberation times
• Comparison of speech intelligibility performance with subjective measures of quality
• Comparison of human performance with engineering metrics
Direct Evidence for Modular Binding Interactions between Cell Adhesion Molecules
The Structures of Many Adhesion Proteins Consist of Multiple Tandem Repeats of
Similar Domains
How Does This Modular Architecture Impact Protein-
mediated Intercellular Adhesion?
Cadherins Mediate Cell-Cell Adhesion in All Soft Tissue
• Cadherin extracellular regions comprise 5 similar domains.• Adhesion was predicted to involve only the outermost domain
The Surface Force Apparatus was used to Quantify the Force-Distance Profiles
Between Cadherins on Opposed Surfaces
Force-distance measurements show that cadherin binds in any of three different, antiparallel alignments.
Adhesion involves multiple domains.
Multiple binding interactions may facilitate the sequential assembly
of tight cell-cell junctions.
Sequential interactions impede the abrupt failure of adhesive
junctions under force.
Cadherin monolayers detach in an unusual way
Conclusions
• Cadherin binds in multiple, antiparallel alignments
• This is a consequence of the modular domain structure of the cadherin extracellular region
• These multiple binding interactions may (1) facilitate the self-assembly of adhesive junctions and (2) impede their abrupt failure under force.