Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant...
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![Page 1: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/1.jpg)
Amy Alter Erika Pike
Lake Shore High School
![Page 2: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/2.jpg)
Background Information
• Elodea densa– Water plant
• Carassius auratus– Common goldfish
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Background Information
• Nitrogen cycle
Fish Waste NO2
Nitrite
NO3
Nitrate
Plant Fertilizer
Nitrate Buildup
NO3
Ammonia
Plant Waste
![Page 4: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/4.jpg)
Problem Statement
• How does the presence of a goldfish (Carassius auratus) affect the percent of chloroplasts in Elodea densa?
![Page 5: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/5.jpg)
Hypothesis
• If a goldfish (Carassius auratus) is placed with Elodea (Elodea densa), then the elodea will have more chloroplasts than elodea grown without a goldfish
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Materials
• 60 Goldfish• 84 Regular-Quart
Mason Jars• 150 Pieces of Elodea
densa• Centrifuge• 0.5M Sucrose Solution• Metric Balance• Mortar
• Pestle• Cheese Cloth• Glass Funnel• Centrifuge Tubes• Sharp Sand• 25mL-Graduated
Cylinder• 10mL-Graduated
Cylinder
![Page 7: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/7.jpg)
Goldfish
![Page 8: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/8.jpg)
Materials
• 60 Goldfish• 60 Regular-Quart
Mason Jars• 150 Pieces of Elodea
densa• Centrifuge• 0.5M Sucrose Solution• Metric Balance• Mortar
• Pestle
![Page 9: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/9.jpg)
Mortar and Pestle
![Page 10: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/10.jpg)
Cheese Cloth
![Page 11: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/11.jpg)
Glass Funnel
![Page 12: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/12.jpg)
Materials
• 60 Goldfish• 60 Regular-Quart
Mason Jars• 150 Pieces of Elodea
densa• Centrifuge• 0.5M Sucrose Solution• Metric Balance• Mortar
• Pestle• Cheese Cloth• Glass Funnel• Centrifuge Tubes• Sharp Sand• 25mL-Graduated
Cylinder• 10mL-Graduated
Cylinder
![Page 13: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/13.jpg)
10mL-Graduated Cylinder
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ProcedureJar Preparation
1. Label 30 jars with “Control” and the jar number, 30 jars with “Experimental” and the jar number, and 24 jars with “Experimental extra” and the jar number
2. Dechlorinate tap water following the directions on the bottle of Insto-Chlor and add 1 quart (800mL) of water to each jar.
3. Mass out approximately 4.0g of Elodea and place in all jars
4. Mass out the goldfish, place goldfish weighing between 1.0 and 2.0 grams in all 30 of the experimental jar and all 24 of the experimental extra jars
5. Place all 60 jars near a light source, such as under a light or near a window.
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Procedure1. Place centrifuge tubes, graduated cylinders,
and sucrose solution in an ice bath to keep them cold
2. Mass the test tube to be used in the trial3. Grind plant into a paste
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Procedure
4. Slowly add sucrose solution
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Procedure
5. Filter the pulp into centrifuge tube
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Procedure
6. Place the centrifuge tube in the centrifuge
7. Spin for five minutes
8. Decant the top 5mL
9. Spin for fifteen minutes
10. Pour off any remaining liquid
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Procedure
11. Mass the test tube with the chloroplast pellet
12. Subtract the mass of the test tube from the mass of the test tube with the pellet to find out the weight of the chloroplast pellet
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Data and Observations
• Day 7: all Experimental and Control jars moved
• After day 11, when fish died the jar was removed and replaced by an experimental extra jar
• All fish were fed approximately every other day
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Data and Observations
• Plants in experimental jars were greener and crisper than those in the control jars– The pulp was also greener that the pulp of the
control jars
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Data Analysis and Interpretation
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Data Analysis and Interpretation
• t=((mean1)(mean2))/√(((SD1)2/N1)((SD2)2/N2))
• Our Formula– t=((6.07)(4.97))/√(((4.31)2/30)((1.68)2/30))
• null hypothesis (H0)
– µ1= µ2
• Alternative hypothesis (Ha)
– µ1< µ2
![Page 24: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/24.jpg)
Error
• Lack of time– Bacteria did not build up to complete the
nitrogen cycle• Ammonia build-up
• Insufficient light
• Centrifuge speed
• Fish are not predictable
• Feeder goldfish
![Page 25: Amy Alter Erika Pike Lake Shore High School. Background Information Elodea densa –Water plant Carassius auratus –Common goldfish.](https://reader033.fdocuments.us/reader033/viewer/2022051215/5697bf951a28abf838c90f70/html5/thumbnails/25.jpg)
Conclusion
• How does the presence of a goldfish (Carassius auratus) affect the percent of chloroplasts in Elodea densa?
• If a goldfish (Carassius auratus) is
placed with Elodea (Elodea densa), then
the elodea will have more chloroplasts
than elodea grown without a goldfish
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Real Life Applications
• Low-maintenance planted aquariums
• Nitrogen Cycle
• Fertilizers
• Goldfish’s ability to survive in small environments
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Further Experimentation
• Different fish with the same set-up– Betta splenden
• Larger containers
• Filtration on containers
• Different comparisons– Fertilizers– Fish
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Acknowledgements
• Ms. DeMaria
• Mrs. Moore
• Ms. Pike
• Cynthia Anthony
• Mr. and Mrs. Schroeder
• Mr. Supal
• Mr. Estapa
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Picture Acknowledgements
• http://www.dewinters.nl/english/fishes/Betta%20splendens.htm
• http://www.goldfishutopia.net/gallery/kablah/• http://www.petsmart.com/global/product_detail.jsp?
PRODUCT%3C%3Eprd_id=845524441776829&FOLDER%3C%3Efolder_id=2534374302030112&ASSORTMENT%3C%3East_id=2534374302023693&bmUID=1104255833546
• http://www.hoftiezer.net/personal/aquaria/images/20030428_front_04_small.jpg
• http://www.bigalsonline.com/catalog/product.xml?product_id=28603&category_id=3349&pcid1=
• http://www.hebs.scot.nhs.uk/library/img/chiMaA01.jpg