Amino Acid Ppt
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Transcript of Amino Acid Ppt
AMINO ACID DEFICIENCY
DISEASES IN FISHES
By-Amruta SheteAQC-PA1-04
INTRODUCTION• The information on protein requirement is of
limited value unless amino acids requirement of fish is understood.
• Amino acids are the organic compound containing an amino group (NH2), a carboxylic acid group (COOH), and any of various side groups (R).
• Link together by peptide bonds to form proteins or that function as chemical messengers and as intermediates in metabolism.
Protein in body tissues incorporate about 20 amino acids viz, essential amino acids (EAA) and non-essential amino acids.
Out of which 10 amino acids (EAA) must be supplied in the diet since fish cannot synthesise
them. Sr. No. Essential Amino Acids
1 Isoleucine
2 Leucine
3 Methionine
4 Phenylalanine
5 Threonine
6 Tryptophan
7 Valine
8 Histidine
9 Tyrosine
10 Lysine
Amino acids are required for the maintenance, growth, reproduction and repletion of tissues
Reduced or abnormal amino acid intake in fish/shrimp results in reduced biosynthesis of many vital substances: enzymes, hormones, certain pigments, and cofactors
Certain amino acids are necessary for oxidation and utilization of fats and carbohydrates
CAUSE FOR DIETARY EAA DEFICIENCIES
1. Poor feed formulation due to the use of disproportionate amounts of feed proteins with natural specific EAA deficiencies
LIMITING ESSENTIAL AMINO ACIDS OF SELECTED PROTEIN SOURCES
S.no Feedstuff LAA S.no Feedstuff LAA
1 Cottonseed Lys 7 Fish muscle Phe
2 Sunflower Lys 8Fish meal (herring) Thr
3 Linseed Lys 9 Fish meal (white) Thr
4 Groundnut Met 10Fish protein concentrate Cys
5 Rapeseed Cys 11 Fish silage Trp
6 Soybean Met 12 Whole shrimp meal
His
2. Presence of disproportionate levels of specific amino acids in the protein source.
Ex.-Blood meal is a rich source of valine, leucine and histidine, but is a very poor source of methionine and isoleucine. Therefore, animals fed high dietary levels of blood meal suffers from an isoleucine deficiency caused by an excess of dietary leucine (Taylor, Cole & Lewis, 1977).
3. Due to excessive heat treatment of feed proteins during feed manufacture.
Ex. -Excessive heat treatment in fish meal production has been shown to markedly reduce protein digestibility and biological value due to the destruction of amino acids by oxidation or through the formation of linkages between individual amino acids which are more resistant to digestion ( Pike, Andorsdottir & Mundheim, 1990).
4. Dietary EAA deficiencies may arise from the chemical treatment of feed proteins with acids (silage production) or alkalies, due to the loss of free tryptophan and lysine/cystine respectively (Kies, 1981).
5. Dietary EAA deficiencies may arise from the leaching of free and protein bound amino acids into the water. Grabner, Wieser & Lackner (1981), reported
the loss, through leaching, of almost all the free and about one-third of the free plus protein bound amino acids from frozen or freeze-dried zooplankton (Artemia Salina and Moina spp.) after a ten minute immersion period at 9°C.
PATHOLOGICAL SIGNS FOR DIETARY EAA DEFICIENCIES
Poor FCR, FCEAnaemia or low blood erythrocyte countReduction of growthScoliosis and lordosisCataracts
DISEASES DUE TO DEFICIENCY OF EAA
EAA Fish species Deficiency signs
References
Lysine Oncorhynchus mykiss
-Dorsal/caudal fin erosion -increased mortality
Walton, Cowey & Adron (1984) Ketola (1983)
Cyprinus carpio Increased mortality
Mazid et al. (1978),
EAA Fish species Deficiency signs
References
Methionine O. mykiss Cataract
Walton,Cowey & Adron (1982)Cowey et el.(1992)
Salmo salar CataractBarash, Poston &Rumsey (1982)
EAA Fish species Deficiency signs References
TryptophanOncorhynchus
mykiss
1. Scoliosis2. lordosis 3. renal calcinosis 4. cataract 5. caudal fin
erosion, decreased carcass lipid content
6. elevated Ca, Mag, Na and K carcass concentration
•Walton et al. (1984)
• Kloppel & Post (1975)
Scoliosis Halver & Shanks (1960)
O.nerka Scoliosis cataract
Akiyama, Mori & Murai (1986)
O.keta O. kisutch Scoliosis
Also deficiency of sulfur containing AA's causes cataractExcesses of leucine or isoleucine increases valine requirement (antagonism)In severe cases, deficiency reduces the ability to resist diseases and lowers the effectiveness of the immune response mechanism.
EAA Fish species Deficiency signs References
Miscellaneous C. carpio
Increased mortality and incidence of
lordosis observed with dietary deficiencies of
leucine, isoleucine, lysine, arginine and
histidine
Mazid et al. (1978)
Lordosis
scoliosis
Cataract
Fin Erosion
TOXIC AMINO ACIDS Plant legumes Leucaena leucocephala (toxic non-
protein amino acid – mimosine, Wee & Wang, 1987)
Sesbania grandiflora and Canavalia ensiformis
(toxic amino acid - L-canavanine; Olvera et al. 1988).
EAA (ie. leucine) have also been reported to exert a toxic effect in fish when present in dietary excesses (Robinson, Poe & Wilson, 1984). For example, the reported toxicity signs for a dietary excess of leucine (13.4% of diet) in rainbow trout (O. mykiss) included scoliosis, deformed opercula, scale deformities, scale loss (Choo et al. 1991).
ESSENTIAL AMINO ACID REQUIREMENTS OF SOME FISH SPECIES (% DIETARY PROTEIN)
Amino acids
Rohu Catla mrigal Common carp tilapia Rainbo
w troutMilkfish
Arginine 5.75 4.80 5.25 4.3 4.2 5.0 5.2
Histidine 2.25 2.45 2.13 2.1 1.7 1.8 2.0
Isoleucine 3.0 2.35 2.75 2.5 3.1 2.0 4.0
Leucine 4.63 3.70 4.25 3.3 3.4 3.5 5.1
Lysine 5.58 6.23 5.88 5.7 5.1 4.5 4.0
Methionine 2.88 3.55 3.18 3.1 2.7 3.5 2.5
Phenylalanine 4.00 3.70 4.00 6.5 5.5 4.5 4.2
Threonine 4.28 4.95 4.13 3.9 3.8 2.0 0.5
Tryptophan 1.13 0.95 1.08 0.8 1.0 0.5 0.6
valine 3.75 3.55 3.50 3.6 2.8 3.2 3.6
AMINO ACID COMPOSITION OF COMMON PROTEIN SOURCES (G/ 100 G PROTEIN).
C P (%)
Met(+Cys
)Lys Trp Thr Ile His Val Leu Ar
g
Phe(+Ty
r)
Fish meal 68 3.1 7.9 1.1 4.0 4.2 8.8 7.9 7.1 8.3 3.6
Soybean meal 48 1.6 6.7 1.3 4.2 5.5 2.7 5.7 8.0 8.0 5.7
Corn gluten meal 60 3.2 1.7 0.5 3.3 3.8 2.0 4.5 15.7 3.
2 6.3
Blood meal 85 1.2 6.3 1.2 4.5 0.9 3.6 6.1 12.2 2.8 6.0
Meat and bone meal 50 1.2 4.9 0.4 4.0 3.8 3.3 5.3 5.7 6.
0 4.0
Poultry by-product meal 65 1.7 5.9 0.9 4.0 2.9 2.2 4.8 5.7 7.
5 2.5
Feather meal 85 0.7 1.2 0.5 3.3 3.1 0.3 5.4 9.2 4.6 3.1
CONCLUSION
Proper diet formulation with appropriate levels of essential amino acids can prevent amino acid deficiency diseases in fishes.
REFERENCES Akiyama,T., K.Mori & T.Murai, (1986) Effects of temperature on the incidence of scoliosis and
cataracts in chum salmon fry caused by tryptophan deficiency.Bull.Jap.Soc.Sci.Fish., 52:2039 Barash, H., H.A.Poston & G.L.Rumsey, (1982) Differentiation of soluble proteins in cataracts
caused by deficiencies of methionine, riboflavin or zinc in diets fed to Atlantic salmon,Salmo salar, rainbow trout, Salmo gairdneri, and lake trout, Salvelinus namaycush. Cornell Vet., 72:361–371
Choo,P-S., et al, (1991) Dietary excesses of leucine influence growth and body composition of rainbow trout. J.Nutr., 121:1932–1939
Cockerell,I., B.J.Francis & D.Halliday, (1972) Changes in the nutritive value of concentrate feeding stuffs during storage. In Proceedings of Conference on Development of feed resource and improvement of animal feeding methods in the CENTO Region countries, Ankara, 1–7 June 1971. London, Tropical Products Institute, pp.181–192
Cowey,C.B.,, (1992) Methionine intake in rainbow trout (Oncorhynchus mykiss), relationship to cataract formation and in the metabolism of methionine. J.Nutr., 122:1154–1163
Grabner,M., W.Wieser & R.Lackner, (1981) The suitability of frozen and freeze-dried zooplankton as food for fish larvae: a biochemical test program. Aquaculture, 26:85–94
Halver,J.E. & W.E.Shanks, (1960) The nutrition of salmonid fishes. 8. Indispensable amino acids for sockeye salmon. J.Nutr., 72:340–348
Holm,J.C. & B.T.Walther, (1988) Free amino acids in live freshwater zooplankton and dry feed: possible importance for first feeding in Atlantic salmon fry (Salmo salar).Aquaculture, 71:223–234
Ichhponani,J.S. & G.N.Lodhi, (1976) Recycling animal waste as feed: A review. Indian Ketola,H.G.,, (1978) Dietary zinc prevents cataract in trout. Fed.Proc.Fed.Am.Soc.Exp.Biol.,
37:584
CONTD… Kies,C., (1981) Bioavailability: A factor in protein quality. J.Agric.Food Chem., 29:435–440 Kloppel,T.M. & G.Post, (1975) Histological alterations in tryptophan-deficient rainbow
trout. J.Nutr., 105:861–866 Mazid, M.A., et al, (1978) Metabolism of amino acids on aquatic animals. III. Indispensable amino
acids for Tilapia zillii. Bull.Jap.Soc.Sci.Fish., 44:739–742 Olvera,N., et al, (1988) The use of the Leguminous plant Sesbania grandiflora as a partial
replacement for fish meal in diets for Tilapia (Oreochromis mossambicus). Aquaculture, 71:51–60 Pike,I.H., G.Andorsdottir & H.Mundheim, (1990) The role of fish meal in diets for salmonids.
International Association of Fish Meal Manufacturers Technical Bulletin No.24, March 1990, London, 35p.
Robinson,E.H., Poe W.E.& Wilson R.P., (1984) Effects of feeding diets containing an imbalance of branched-chain amino acids on fingerling channel catfish. Aquaculture, 37:51–62
Taylor,S.J., J.A.Cole & D.Lewis, (1977) An interaction of leucine, isoleucine and valine in the diet of the growing pig. Proc.Nutr.Soc., 36:36A (Abstr.)
Walton,M.J.,(1984) The effect of dietary lysine levels on growth and metabolism of rainbow trout (Salmo gairdneri). Brit.J.Nutr., 52:115–122
Watanabe,T.,(1981) The relationship between dietary lipid levels and alpha-tocopherol requirement of rainbow trout. Bull.Jap.Soc.Sci.Fish., 47:1463– 1471
Watanabe,T.,(1987) Effect of dietary histidine or histamine on growth and development of stomach erosion in rainbow trout. Bull.Jap.Soc.Sci.Fish., 53:1207–1214
Wee,K.L. & S.S.Wang, (1987) Nutritive value of Leucaena leaf meal in pelleted feed for Nile tilapia. Aquaculture, 62:97–108
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