Amino Acid Analysis Using ZORBAX Eclipse
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Amino Acid Analysis Using ZORBAX Eclipse AAA Columns.
Transcript of Amino Acid Analysis Using ZORBAX Eclipse
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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mAU
0
5
10
min0 4 6 8 10 12
mAU
0
5
10
15
2
1 2
3
4
5
6
78
9
10 11 12
21
2019
18
1716
15
14
13
22
23
2421
A
B
338 nm
262 nm
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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mAU
0
4
8
min0 5 10 15 20 25
mAU
0
2
46
8
1
2
3
4
5
678
9
1011 12 131415
1716
1819
20
3
21
12
4
5
6
7 8
9
1110 12 131415
1617
1819
20
21
5 m, Zorbax Eclipse-AAA
3.5 m Zorbax Eclipse-AAA
mAU
0
5
mAU
0
510
min0 10 15 20 25
LU
0
500
1000
5
1 2
3
4567
8
9
1011 12 13
1415
16171819
2021
21
2223
24
12
3
4
5
6
78
9
1011 12 14
15
161718
24
2322
21
2019
A
B
C
338 nm
262 nm
FLD
mAU
0
2
4
6
8
min0 10 15 20 25
mAU
0
4
8
12
5
1
2
3
4
5
6
78
9
1011 1213
14
15
16
17
18
1920
21
23
24
22
21
338 nm
262 nm
A
B
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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mAU
0
10
20
30
40
min0 5 10 15 20 25
mAU
0
2
4
6
8
A
BAsp/Glu
Asp/Glu
His/Gl
Ala/Arg
Val/Met Phe/Ile
His/Gly
Val/Met
Phe/IleArg/Ala
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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0
1000
2000
3000
4000
5000
0 100 200 300 400 500 600
pmoles
PeakArea
Asp
His
Thr
Cys
Lys
B
0
20
40
60
0 100 200 300 400 500 600
pmoles
PeakArea
Asp
His
Thr
Cys
Lys
A
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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mAU
-1
0
1
2
3
min0 2 4 6 8 10
mAU
-1
0
1
2
3
21
3
4
56 7 8 9
10
11 12 1314 1516
1 2
34
5 6 7 8 910
11
12
1314
1516
5 pmol
50 pmol
LU
0
50
100
150
200
min0 2 4 6 8 10
LU
0
50
100
150
200
21
3
4
5 6
7
8 9
10
1112 1314
15
17
1 2
3
4
56 7 8 9
10
11 12
1314 15 17
5 pmol
50 pmol
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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C
BA
WATER
FMOC
OPA
WATER
AA SAMPLE
BORATE
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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5/27/2018 Amino Acid Analysis Using ZORBAX Eclipse
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