ambr 15 cell culture Performance Data -...
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ambr ® 15 cell culture Performance Data ambr ® 15 cell culture can model growth and productivity in a 10 L bench scale system Efficient Using ambr 15 can dramatically reduce the time spent setting up and running bioreactor experiments. Figure 1: Operator time to set-up and run 24 bioreactor experiments ambr ® 15 is cost-effective, efficient and easy to use. Performance of micro bioreactors is consistent from vessel to vessel. Operator Hours ambr Workstation 24 6x1 L Benchtop system 600 500 400 300 200 100 0 37 607
Transcript of ambr 15 cell culture Performance Data -...
ambr® 15 cell culture
Performance Data
ambr® 15 cell culture can model growth and productivity in a 10 L bench scale system
Efficient
Using ambr 15 can dramatically reduce the time spent setting up and running bioreactor experiments.
Figure 1: Operator time to set-up and run 24 bioreactor experiments
ambr® 15 is cost-effective, efficient and easy to use. Performance of micro bioreactors is consistent from vessel to vessel.
Ope
rato
r Hou
rs
ambr Workstation 24 6x1 L Benchtop system
600
500
400
300
200
100
037
607
Scalable
Growth profiles in ambr 15 show good comparability to those in bench scale bioreactors. Data is from CHO fed batch culture in ambr 15 and a10 L bioreactor.
Figure 3: Data from cell line E
Consistent
The precise control and uniform conditions in each culture result in minimal growth variation from bioreactor to bioreactor and run to run.
Figure 2: Viable cell count from 24 ambr 15 vessels
Viab
le c
ell c
ount
(x10
5 )
100
80
60
40
20
00 2 4 6 8 10 12
Culture time (days)
Viab
le c
ell c
ount
(x10
5 )
25
20
15
10
5
00 50 100 150 200 250 300
Culture time (days)
ambr reactor 1
ambr reactor 2
10 L bioreactor
Specifications subject to change without notice. Printed and copyrighted by Sartorius Stedim Biotech GmbH. | W Publication No. : SBI4003-e141201 Order No. : 85037-548-57 Ver. 02 | 2015
Sartorius Stedim Biotech GmbHAugust-Spindler-Strasse 1137079 Goettingen, GermanyPhone +49.551.308.0Fax +49.551.308.3289www.sartorius-stedim.com
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Specifications subject to change without notice. Printed and copyrighted by Sartorius Stedim Biotech GmbH. | WPublication No.: Order No.: Ver. 01 | 2012
TAP Biosystems is now part of the Sartorius Stedim Biotech Group
Sartorius is grateful to a number of companies that have helped test ambr 15 during the product development process. In particular we thank Dr Colin Jaques and Dr Matthew Cheeks from Lonza Biologics plc, UK for providing the data used in figures 3 and 4.
Fit for purpose
Cell line ranking experiments using ambr 15 can give excellent prediction of performance in large scale bioreactors.
Figure 4: Comparison of clone ranking performance in ambr 15 and 10 L bioreactors
Cell line 10 L bioreactor pg/cell/day
Bioreactor ranking
ambr pg/cell/day
ambr ranking
G 36.2 1 45.6 1
H 31.0 2 29.8 2
J 25.1 3 29.3 3
A 24.8 4 27.3 4
E 20.3 5 24.2 5
B 19.8 6 22.6 6
F 17.4 7 21.1 7
C 8.6 8 10.4 8
I 5.4 9 9.0 9
D 3.6 10 7.2 10
