Ambient Water Toxicity Surveys - Amazon S3 · Salinity (ppt) A. bahia mysid NOEC M. beryllina fish...
Transcript of Ambient Water Toxicity Surveys - Amazon S3 · Salinity (ppt) A. bahia mysid NOEC M. beryllina fish...
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Ambient Water Toxicity SurveysAmbient Water Toxicity Surveys
A. Ronald A. Ronald MacGillivrayMacGillivray, Ph.D., Ph.D.Environmental ToxicologistEnvironmental Toxicologist
[email protected]@drbc.state.nj.us
Delaware Estuary Science Conference 2007Delaware Estuary Science Conference 2007
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Advantages of Advantages of Ambient Water Toxicity TestingAmbient Water Toxicity Testing
•• Assesses point sources and nonAssesses point sources and non--point point sourcessources
•• Assesses mixtures (cumulative effects)Assesses mixtures (cumulative effects)•• Assesses toxicants with no chemical Assesses toxicants with no chemical
specific water quality standards and/or are specific water quality standards and/or are not being monitored by chemical analysisnot being monitored by chemical analysis
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BackgroundBackground
•• DRBC chronic toxicity studies of ambient DRBC chronic toxicity studies of ambient water in 1990, 1992, 1999, 2000, 2001water in 1990, 1992, 1999, 2000, 2001
•• Tidal Delaware River (RM 63 to 115)Tidal Delaware River (RM 63 to 115)•• Twelve fixed stationsTwelve fixed stations
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Previous DRBC Ambient Water Previous DRBC Ambient Water Toxicity StudiesToxicity Studies
•• Survival of all test organisms not affectedSurvival of all test organisms not affected
•• SubSub--lethal chronic toxicity suggested at different lethal chronic toxicity suggested at different sampling stations and in different species over sampling stations and in different species over the study periodthe study period
•• EPA reviews conclude data inconclusive, EPA reviews conclude data inconclusive, recommend further study (possible confounding recommend further study (possible confounding factors e.g., salinity effect)factors e.g., salinity effect)
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Chronic Toxicity WorkgroupChronic Toxicity WorkgroupDeveloping ConsensusDeveloping Consensus
On Test Species and Study DesignOn Test Species and Study Design
•• DNREC DNREC –– Rick GreeneRick Greene•• NJDEP NJDEP –– Betty Jane Betty Jane
BorosBoros--Russo/Michelle Russo/Michelle MooneyMooney
•• DRBC DRBC –– Ron Ron MacGillivrayMacGillivray•• DRBC DRBC –– Tom Tom FikslinFikslin•• EPAEPA––RIII RIII –– Brian Brian TrulearTrulear•• EPAEPA––RII RII –– Jim Jim FerrettiFerretti•• PADEP PADEP –– Mike Boyer
•• EPAEPA--RIII RIII –– Dave RussellDave Russell•• DuPontDuPont, Haskell Lab , Haskell Lab ––
Bob Bob HokeHoke•• Rohm & Haas Co. Rohm & Haas Co. –– Steve Steve
BrownBrown•• PWD PWD –– Linda OLinda O’’DonnellDonnell•• AAT AAT –– Chris Chris NallyNally•• EPA EPA –– ORD consultationORD consultation
Mike Boyer
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ObjectivesObjectives
••To assess if chronic toxicity, as To assess if chronic toxicity, as measured in laboratory controlled measured in laboratory controlled experiments, is present in the river experiments, is present in the river water samples collected.water samples collected.
••To develop appropriate toxicity tests To develop appropriate toxicity tests for the estuary.for the estuary.
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Freshwater Test Species
Ceriodaphnia dubia
water flea
Pimephales promelasfathead minnow
Pseudokirchneriella subcapitata
green algae
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Salinity Tolerant Test Species
Menidia beryllina
inland silverside (5 to 32 ppt)Americamysis bahia
mysid shrimp (10 to 30 ppt)
Hyalella azteca
amphipod (0 to 15 ppt)
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2005 Sites2005 Sitesmidmid--channelchannel
•• June June Survey Survey (sites T4 to (sites T4 to T16)T16)
•• September September Survey Survey (sites T1 to (sites T1 to T7)T7)
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June Survey ConditionsJune Survey Conditionsgraph by Dr. N. graph by Dr. N. SukSuk
Flows and Turbidity for the Delaware River at Trenton
5000
5500
6000
6500
7000
06/05/05 06/06/05 06/07/05 06/08/05 06/09/05 06/10/05 06/11/05
Flow
, CFS
0
5
10
15
20
Tur
bidi
ty, F
TU
Flow Turbidity
Chronic Toxicity Sampling
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September Survey ConditionsSeptember Survey Conditions
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•• NiskinNiskin sampler to analytical lab sample bottlesampler to analytical lab sample bottle
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•• Composite sample Composite sample
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•• Transfer to Transfer to cubitainercubitainer for transport to for transport to toxtox lablab
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Ambient Water Toxicity TestsAmbient Water Toxicity Tests
•• Expose test species to Expose test species to –– 100%, 75%, 50%, 25%, and 12.5% ambient 100%, 75%, 50%, 25%, and 12.5% ambient
waterwater
•• Test Duration Test Duration -- 4 to 10 days (species 4 to 10 days (species specific)specific)
•• Test for survival and growth or Test for survival and growth or reproduction reproduction
•• Compare to controlsCompare to controls
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JuneJuneFreshwaterFreshwater
SitesSites
P. P. promelaspromelasfishfish
NOECNOEC
C. C. dubiadubiainvertebrateinvertebrate
NOECNOEC
P. P. subcapitatasubcapitataalgaealgae
NOECNOEC
T4 S of T4 S of Del Del MemMem BrBr
100%100% 75% 75% reproductionreproduction
(salinity effect?)(salinity effect?)
100%100%
T5 N of T5 N of Del Del MemMem BrBr
100%100% 100%100% 100%100%
T6 T6 OldmanOldman’’ss PtPt
100%100% 100%100% 100%100%
T7 Marcus Hook T7 Marcus Hook CreekCreek
100%100% 100%100% 100%100%
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JuneJuneSitesSites
P. P. promelaspromelasfishfish
NOECNOEC
C. C. dubiadubiainvertebrateinvertebrate
NOECNOEC
P. P. subcapitatasubcapitataalgaealgaeNOECNOEC
T8 T8 EddystoneEddystone 100%100% 100%100% 100%100%
T9 S of T9 S of SchuykillSchuykill RR
100%100% 100%100% 100%100%
T10 at Big T10 at Big Timber CreekTimber Creek
100%100% 100%100% 100%100%
T11 PennT11 Penn’’s s LandingLanding
100%100% 100%100% 100%100%
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JuneJuneSitesSites
P. P. promelaspromelasfishfish
NOECNOEC
C. C. dubiadubiainvertebrateinvertebrate
NOECNOEC
P. P. subcapitatasubcapitataalgaealgaeNOECNOEC
T12 at T12 at Pennsauken CreekPennsauken Creek
100%100% 100%100% 100%100%
T13 atT13 atRancocasRancocas CreekCreek
100%100% 100%100% 100%100%
T14 BeverlyT14 Beverly 100%100% 100%100% 100%100%
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JuneJuneSitesSites
P. P. promelaspromelasfishfish
NOECNOEC
C. C. dubiadubiainvertebrateinvertebrate
NOECNOEC
P. P. subcapitatasubcapitataalgaealgaeNOECNOEC
T15 FlorenceT15 Florence 100%100% 100%100% 100%100%
T16 T16 BilesBilesChannelChannel
100%100% 100%100% Single Single Concentration Concentration NOEC <100%NOEC <100%
ResampledResampled three three weeks later with weeks later with Dilution Series Dilution Series NOEC = 100%NOEC = 100%
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SeptemberSeptemberSites and Sites and Salinity (Salinity (pptppt))
A. A. bahiabahiamysidmysidNOECNOEC
M. M. beryllinaberyllinafishfish
NOECNOEC
H. H. AztecaAztecaamphipodamphipod
NOECNOEC
T1 T1 ListonListon PtPt11.4 11.4 -- 15.515.5
100%100% 100%100% 100%100%
T2T2Reedy IsReedy Is8.2 8.2 -- 10.410.4
100%100% 100%100% 100%100%
T3T3N of Pea Patch N of Pea Patch IsIs5.8 5.8 -- 6.46.4
100%100% 100%100% 100%100%
T4 S of Del T4 S of Del MemMemBrBr4.3 4.3 -- 4.94.9
100%100% 100%100% 100%100%
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SeptemberSeptemberSites and Sites and Salinity (Salinity (pptppt))
A. A. bahiabahiamysidmysidNOECNOEC
M. M. beryllinaberyllinafishfish
NOECNOEC
H. H. AztecaAztecaamphipodamphipod
NOECNOEC
T5 N of Del T5 N of Del MemMem BrBr3.4 3.4 –– 3.93.9
100%100% 100%100% 100%100%
T6 T6 OldmanOldman’’ss PtPt2.2 2.2 –– 3.13.1
100%100% 100%100% 100%100%
T7 Marcus Hook T7 Marcus Hook CreekCreek0.9 0.9 –– 1.61.6
75% growth75% growthICIC2525 98.8 growth98.8 growth
100%100% 100%100%
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ConclusionsConclusions
•• Identified a set of test species and modified toxicity Identified a set of test species and modified toxicity testing methods appropriate for routine monitoring of testing methods appropriate for routine monitoring of the estuary.the estuary.
•• The June and September 2005 toxicity surveys The June and September 2005 toxicity surveys indicated overall, based on the measured toxicity indicated overall, based on the measured toxicity endpoints, that the ambient water samples collected endpoints, that the ambient water samples collected were not chronically toxic.were not chronically toxic.
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Future DirectionFuture Direction
•• Shift from a developmental phase of Shift from a developmental phase of identifying appropriate test species and identifying appropriate test species and methods to a monitoring phase with a methods to a monitoring phase with a continued focus on ambient toxicity testing continued focus on ambient toxicity testing of water.of water.
•• Increase spatial and temporal coverage of Increase spatial and temporal coverage of the estuary (new focus on tributaries as well the estuary (new focus on tributaries as well as mainas main--stem)stem)
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CriteriaCriteria forfor Prioritization of 2007 Prioritization of 2007 Tributary SamplingTributary Sampling
•• Direct tributary to the Delaware Estuary in Direct tributary to the Delaware Estuary in Zones 2 through 5Zones 2 through 5
•• Segment of the tributary is listed for toxics Segment of the tributary is listed for toxics in a state Integrated Assessmentin a state Integrated Assessment
•• History of sampling as part of a tributary History of sampling as part of a tributary monitoring programmonitoring program
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2007
Ambient
Toxicity
Survey
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Water Quality Water Quality
•• Dissolved cadmium, chromium Dissolved cadmium, chromium ((hexavalenthexavalent and total), lead, nickel, silver and total), lead, nickel, silver and zinc did not exceed DRBC Water and zinc did not exceed DRBC Water Quality Objectives for the limited number Quality Objectives for the limited number of sites and dates tested. of sites and dates tested.
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Ambient Copper Concentrations at Selected Sites Ambient Copper Concentrations at Selected Sites June 2005 surveyJune 2005 survey
DateDate SiteSite Current Current USEPA USEPA CriteriaCriteriaµµg/Lg/L
Proposed Proposed USEPA USEPA CriteriaCriteriaµµg/Lg/L
Measured Measured Dissolved Dissolved
CuCuµµg/Lg/L
Applicable Applicable DRBCDRBC
Regulatory Regulatory CriteriaCriteriaµµg/L g/L
BLM BLM SiteSite--
specific specific Chronic Chronic WQC WQC µµg/Lg/L
6/6/056/6/05 T4T4 1313
4.84.8
4.84.8
1313
4.84.8
1313
4.84.8
2.12.1 2.82.8 7.87.8
6/6/056/6/05 T5T5
3.13.1
4.24.2
4.24.2
3.13.1
4.24.2
3.13.1
22 8.38.3 7.67.6
6/6/056/6/05 T16T16 <1.4<1.4 8.38.3 16.816.8
6/8/056/8/05 T4T4 2.12.1 2.82.8 11.111.1
6/8/056/8/05 T5T5 22 8.38.3 12.912.9
6/10/056/10/05 T4T4 2.12.1 2.82.8 12.412.4
6/10/056/10/05 T5T5 4.24.2 22 8.38.3 27.027.0
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Water Quality Water Quality
•• Dissolved copper did not exceed the Dissolved copper did not exceed the applicable regulatory criteria or the siteapplicable regulatory criteria or the site--specific water quality criteria predicted by specific water quality criteria predicted by the BLM for the sites and dates evaluated.the BLM for the sites and dates evaluated.
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ReferencesReferences
•• U.S. Environmental Protection Agency. 2000. Section 11. U.S. Environmental Protection Agency. 2000. Section 11. Test Method 100.1. Test Method 100.1. HyalellaHyalella aztecaazteca 1010--d Survival and d Survival and Growth Test for Sediments. EPA 600/RGrowth Test for Sediments. EPA 600/R--99/064.99/064.
•• U.S. Environmental Protection Agency, October 2002. U.S. Environmental Protection Agency, October 2002. ShortShort--Term Methods For Estimating The Chronic Toxicity Term Methods For Estimating The Chronic Toxicity Of Effluents And Receiving Waters To Freshwater Of Effluents And Receiving Waters To Freshwater Organisms, Fourth Edition EPAOrganisms, Fourth Edition EPA--821821--RR--0202--013.013.
•• U.S. Environmental Protection Agency, October 2002. U.S. Environmental Protection Agency, October 2002. ShortShort--Term Methods For Estimating The Chronic Toxicity Term Methods For Estimating The Chronic Toxicity Of Effluents And Receiving Waters To Marine & Estuarine Of Effluents And Receiving Waters To Marine & Estuarine Organisms, Third Edition EPAOrganisms, Third Edition EPA--821821--RR--0202--014.014.