Alisa Sue Wolberg, Ph.D. EDUCATION - UNC Health Care · Curriculum Vitae . Alisa Sue Wolberg, Ph.D....

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Curriculum Vitae

Alisa Sue Wolberg, Ph.D.

University of North Carolina, Chapel Hill Phone: (919) 966 – 8430 Department of Pathology and Laboratory Medicine, CB#7525 Fax: (919) 966 – 6718 Chapel Hill, NC 27599-7525 Email: [email protected] EDUCATION Research Associate (Post-Doctoral Training), Department of Pathology, Duke University, Durham, NC:

11/1996 – 6/2001, Research in Blood Coagulation Doctor of Philosophy, Department of Biology, University of North Carolina, Chapel Hill, NC: 8/1991 –

12/1996; Thesis: Characterization of Human Coagulation Factor IX; Advisor: Dr. Darrel W. Stafford, Professor of Biology

Bachelor of Science, Department of Biology, University of North Carolina, Chapel Hill, NC: 8/1987 – 5/1991 (Biology), Minor from the Department of Chemistry

PROFESSIONAL EXPERIENCE - EMPLOYMENT HISTORY Associate Professor with Tenure, Department of Pathology and Laboratory Medicine, University of

North Carolina at Chapel Hill: 7/2011 – present Assistant Professor, Department of Pathology and Laboratory Medicine, University of North Carolina at

Chapel Hill: 4/2007 – 6/2011 Research Assistant Professor, Department of Pathology and Laboratory Medicine, University of North

Carolina at Chapel Hill: 12/2001 – 3/2007 Assistant Research Professor, Department of Pathology, Duke University: 7/2001 – 11/2001 Summer Intern, Burroughs Wellcome: 5/1990 – 8/1990, 5/1991 – 8/1991 HONORS and AWARDS Fellow, American Heart Association, 2011 Finalist, Kenneth M. Brinkhous Young Investigator Prize in Thrombosis, 2009 Joe W. Grisham Award for Excellence in Graduate Student Teaching, 2008 Novo Nordisk Award “In recognition of outstanding research of an original nature leading to further

understanding of bleeding disorders and their treatment,” 2005 Associate, Building Interdisciplinary Research Careers in Women’s Health (BIRCWH), University of

North Carolina at Chapel Hill: 7/2004 – present Scholar, Building Interdisciplinary Research Careers in Women’s Health (BIRCWH), University of

North Carolina at Chapel Hill: 11/2003 – 7/2004 Bayer Corporate Fellow, University of North Carolina at Chapel Hill: 12/2001 – 6/2002 American Society of Hematology Travel Award, 1999 University of North Carolina, Graduate School Travel Award, 1993

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BIBLIOGRAPHY REFEREED PAPERS AND ARTICLES IN PEER REVIEWED JOURNALS

1. Rita Marchi, Bethany L. Walton, Colleen S. McGary, Feng-Chang Lin, Alice D. Ma, Rafal Pawlinski, Nigel Mackman, Robert A. Campbell, Jorge Di Paola, Alisa S. Wolberg*. 2012. Dysregulated coagulation from hypofibrinogenemia and elevated factor VIII levels. Thrombosis and Haemostasis, in press.

2. Alisa S. Wolberg*. 2012. Determinants of fibrin formation, structure, and function. Current Opinions in Hematology. In press.

3. Jian-Guo Wang, Julia E. Geddings, Maria M. Aleman, Jessica C. Cardenas, Pichika Chantrathammachart, Julie C. Williams, Daniel Kirchhofer, Vladimir Y. Bogdanov, Ronald R. Bach, Janusz Rak, Frank C. Church, Alisa S. Wolberg, Rafal Pawlinski, Nigel S. Key, Jen-Jen Yeh and Nigel Mackman. 2012. Human pancreatic cancer cell tissue factor activates coagulation in a mouse model. Blood, in press. PMID: 22547577

4. Amy L. Oldenburg, Gongting Wu, Dmitry Spivak, Frank Tsui, Alisa S. Wolberg, Thomas H. Fischer. 2012. Imaging and elastometry of blood clots using magnetomotive optical coherence tomography and labeled platelets. IEEE J Selected Topics In Quantum Electronics, 18(3):1100-9.

5. Alisa S. Wolberg*, Alan E. Mast. 2012. Tissue factor and factor VIIa – Hemostasis and Beyond. Thrombosis Research, 129(Suppl 2):S1-S4. PMID: 22417944

6. Alisa S. Wolberg*. 2012. Aβ and C(lot), but not D(egradation). Blood, 119(14):3196-7. PMID: 22493214

7. Alisa S. Wolberg*, Maria M. Aleman, Karin Leiderman, Kellie R. Machlus. 2012. Procoagulant activity in hemostasis and thrombosis: Virchow’s Triad revisited. Anesthesia & Analgesia, 114(2):275-85. PMID: 22104070

8. Gongting Wu, Alisa S. Wolberg, Amy L. Oldenburg. 2012. Validation study toward measuring the mechanical properties of blood clots using resonant acoustic spectroscopy with optical vibrometry. Proceedings of SPIE Medical Imaging, 8214:82140G. PMID: 22506093

9. Maria M. Aleman, Chris Gardiner, Paul Harrison, Alisa S. Wolberg*. 2011. Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability. Journal of Thrombosis and Haemostasis, 9(11):2251-61. PMID: 21883880

10. Kellie R. Machlus, Feng-Chang Lin, Alisa S. Wolberg*. 2011. Procoagulant activity induced by vascular injury determines contribution of elevated factor VIII to thrombosis and thrombus stability in mice. Blood, 118(14):3960-8. PMID: 21828144

11. Subcommittee on Control of Anticoagulation of the SSC of the ISTH: Trevor Baglin, Martin Besser, M Cattaneo, Yesim Dargaud, Elaine Gray, Nigel S. Key, Thomas Lecompte, Roger Luddington, Sirak Petros, Thomas Siegemund, Alisa S. Wolberg. 2011. Towards a recommendation for standardization for measurement of platelet-dependent thrombin generation. Journal of Thrombosis and Haemostasis, 9(9):1859-61. PMID: 21884566

12. Kellie R. Machlus, Jessica C. Cardenas, Frank C. Church, Alisa S. Wolberg*. 2011. Causal relationship between hyperfibrinogenemia, thrombosis, and resistance to thrombolysis in mice. Blood, 117(18):4953-63. PMID: 21355090. [Commentary by RAS Ariens. 2011. Elevated fibrinogen causes thrombosis. Blood, 117(18):4687-8.]

13. Richard Chasen Spero, Rachel K Sircar, Ryan Schubert, Russell M. Taylor II, Alisa S. Wolberg, Richard Superfine. 2011. Nanoparticle diffusion measures bulk clot permeability. Biophysical Journal, 101(4):943-50. PMID: 21843486

14. Kellie R. Machlus, Maria M. Aleman, Alisa S. Wolberg*. 2011. Update on venous thromboembolism: risk factors, mechanisms, and treatments. Arteriosclerosis, Thrombosis and Vascular Biology, 31(3):476-8. PMID: 21325668

15. Laura D. Gray, Michael A. Hussey, Brittany M. Larson, Kellie R. Machlus, Robert A. Campbell, Gary G. Koch, Mirella Ezban, Ulla Hedner, Alisa S. Wolberg*. 2011. Recombinant factor VIIa

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analog NN1731 (V158D/E296V/M298Q-FVIIa) enhances fibrin formation, structure and stability in lipidated hemophilic plasma. Thrombosis Research, 128:570-6. PMID: 21561645

16. Eungjun Kim, Oleg Kim, Kellie R. Machlus, Timur Kupaev, Joshua Lioi, Jian Mu, Xiaomin Liu, Alisa S. Wolberg, Danny Z. Chen, Elliot D. Rosen, Zhiliang Xu, Mark Alber. 2011. Correlation between fibrin network structure and mechanical properties: an experimental and computational analysis. Soft Matter, 7(10):4983-92.

17. Robert A. Campbell#, Maria M. Aleman#, Laura D. Gray, Michael R. Falvo, Alisa S. Wolberg*. 2010. Flow profoundly influences fibrin network structure: implications for fibrin formation and clot stability in hemostasis. #Co-first authors, Thrombosis and Haemostasis, 104(6):1281-1284. PMID: 20886193

18. Jennifer C. Carter, Robert A. Campbell, Jennifer A. Gibbons, Mark W. Gramling, Alisa S. Wolberg*, Frank C. Church*. 2010. Enhanced cell-associated plasminogen activator pathway but not coagulation pathway activity contributes to motility in metastatic breast cancer cells. Journal of Thrombosis and Haemostasis, 8:1323-32. PMID: 20180817

19. Feng Li, Chih-Hong Wang, Jianquo Wang, Tiffany Thai, Gunnar Boysen, Longquan Xu, Adam L. Turner, Alisa S. Wolberg, Nigel Mackman, Nobuyo Maeda, Nobuyuki Takahashi. 2010. Elevated tissue factor expression contributes to exacerbated diabetic nephropathy in mice lacking eNOS fed a high fat diet. Journal of Thrombosis and Haemostasis, 8(10): 2122-2132. PMID: 20626618

20. Veronique Ollivier, Jian-Guo Wang, David Manly, Kellie R. Machlus, Alisa S. Wolberg, Martine Jandrot-Perrus, Nigel Mackman. 2010. Detection of endogenous tissue factor levels in plasma using the calibrated automated thrombogram assay. Thrombosis Research. 125(1):90-6. PMID: 19345399

21. Alisa S. Wolberg*, Maria M. Aleman. 2010. Influence of cellular and plasma procoagulant activity on the fibrin network. Thrombosis Research. 125(Suppl 1):S35-S37. PMID: 20163831

22. Robert A. Campbell, Kellie R. Machlus, Alisa S. Wolberg*. 2010. Smoking out the cause of thrombosis. Arteriosclerosis, Thrombosis and Vascular Biology. 30(1):7-8. PMID: 20018940

23. Alisa S. Wolberg*. 2010. Plasma and cellular contributions to fibrin network formation, structure, and stability. Haemophilia. 16(Suppl 3):7-12. PMID: 20586795

24. Robert A. Campbell, Katherine A. Overmyer, Brett C. Sheridan, Craig H. Selzman, Alisa S. Wolberg*. 2009. Contributions of extravascular and intravascular cells to fibrin network formation, structure, and stability. Blood, 114(23):4886-96. PMID: 19797520

25. Kellie R. Machlus, Emily A. Colby, Jogin R. Wu, Gary G. Koch, Nigel S. Key, Alisa S. Wolberg*. 2009. Effects of tissue factor, thrombomodulin, and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thrombosis and Haemostasis, 102(5):936-44. PMID: 19888532

26. Alisa S. Wolberg, Nigel Mackman. 2009. Venous thromboembolism: risk factors, diagnosis and treatment. Arteriosclerosis, Thrombosis and Vascular Biology. 29(3):296-7. PMID: 19228601

27. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg*. 2008. Cellular procoagulant activities dictate clot structure as a function of distance from the cell. Arteriosclerosis, Thrombosis, and Vascular Biology, 28(12):2247-54. PMID: 18974382

28. David J. Bautz, Gloria A. Preston, Sofia Lionaki, Peter Hewins, Alisa S. Wolberg, Jia Jin Yang, Susan L. Hogan, Hyunsook Chin, Stephan Moll, J. Charles Jennette, Ronald J. Falk. 2008. Antibodies with dual reactivity to plasminogen and complementary PR3 in PR3-ANCA vasculitis. Journal of the American Society of Nephrology, 19(12):2421-9. PMID: 18701607

29. Thomas H. Fischer, Alisa S. Wolberg, Arthur P. Bode, Timothy C. Nichols. 2008. The interaction of factor VIIa with rehydrated, lyophilized platelets. Platelets. 19(3):182-91. PMID: 18432519

30. Alisa S. Wolberg*. 2008. Polyphosphate: Physiologic? Pathologic? Pharmacologic? Blood. 112(7):2598-9. PMID: 18809768

31. Alisa S. Wolberg*, Robert A. Campbell. 2008. Thrombin generation, fibrin clot formation, and hemostasis. Journal of Transfusion and Apheresis Science. 38(1):15-23. PMID: 18282807

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32. Robert A. Campbell, Thomas H. Fischer, Alisa S. Wolberg*. 2007. A novel approach to improving rFVIIa activity with a preserved platelet preparation. British Journal of Haematology. 138(1):82-93. PMID: 17555451

33. Geoffrey A. Allen, Egon Persson, Robert A. Campbell, Mirella Ezban, Ulla Hedner, Alisa S. Wolberg*. 2007. A variant of recombinant factor VIIa with enhanced procoagulant and antifibrinolytic activities in a model of hemophilia. Arteriosclerosis, Thrombosis, and Vascular Biology, 27(3):683-9. PMID: 17204663

34. Alisa S. Wolberg*. 2007. Antiphospholipid antibody effects on monocytes. Current Rheumatology Reports, 9(3):198-204. PMID: 17531172

35. Alisa S. Wolberg*. 2007. Thrombin generation and fibrin clot structure. Blood Reviews, 21(3):131-42. PMID: 17208341

36. Alisa S. Wolberg*. 2007. Thrombin generation assays: Understanding how the method influences the results. Thrombosis Research, 119(6):663-5. PMID: 16926045

37. Alisa S. Wolberg*, Dougald M. Monroe, Ulla Hedner, Harold R. Roberts, Maureane Hoffman. 2005. High dose factor VIIa improves clot structure and stability in a model of hemophilia B. British Journal of Haematology, 131(5):645-55. PMID: 16351642

38. Alisa S. Wolberg, Robert A. S. Roubey. 2005. Annexin A2: better left alone. Blood, 105(5):1845-6. PMID: 15747402

39. Hong Zhou, Alisa S. Wolberg, Robert A. S. Roubey. 2004. Characterization of monocyte tissue factor activity induced by IgG antiphospholipid antibodies and inhibition by dilazep. Blood, 104(8):2353-8. PMID: 15226179

40. Alisa S. Wolberg, Zhi Hong Meng, Dougald M. Monroe, Maureane Hoffman. 2004. A systematic evaluation of the effect of temperature on coagulation enzyme activity and platelet function. Journal of Trauma. 56(6):1221-8. PMID: 15211129

41. Alisa S. Wolberg, Robert A. S. Roubey. 2004. Mechanisms of autoantibody-induced monocyte tissue factor expression. Thrombosis Research, 114(5-6):391-6. PMID: 15507269

42. Alisa S. Wolberg*, Robert A. S. Roubey. 2004. Anti-beta-2 glycoprotein I antibodies do not increase TF activity by de-encryption. Thrombosis Research, 114(1):67-71. PMID: 15262487

43. Geoffrey A. Allen, Alisa S. Wolberg, Julie A. Oliver, Maureane Hoffman, Harold R. Roberts, Dougald M. Monroe. 2004. Impact of procoagulant concentration on rate, peak and total thrombin generation in a model system. Journal of Thrombosis and Hemostasis, 2(3):402-13. PMID: 15009455. [Commentary by HC Hemker and S Béguin. 2007. Journal of Thrombosis and Hemostasis, 2(3):400-1.]

44. Alisa S. Wolberg, Dougald M. Monroe, Harold R. Roberts, Maureane Hoffman. 2003. Elevated prothrombin results in clots with an altered fiber structure: a possible mechanism of the increased thrombotic risk. Blood. 101(8):3008-13. PMID: 12506014

45. Zhi Hong Meng, Alisa S. Wolberg, Dougald M. Monroe, Maureane Hoffman. 2003. The effect of temperature and pH on the activity of factor VIIa: Implications for the efficacy of high-dose factor VIIa in hypothermic and acidotic patients. Journal of Trauma. 55(5):886-91. PMID: 14608161

46. Derrick Sauls, Alisa S. Wolberg, Maureane Hoffman. 2003. Elevated plasma homocysteine leads to alterations in fibrinogen function and fibrin structure: Implications for the mechanism of the hypercoagulable state in hyperhomocysteinemia. Journal of Thrombosis and Haemostasis. 1(2):300-6. PMID: 12871504.

47. Alisa S. Wolberg, Don A. Gabriel, Maureane Hoffman. 2002. Analyzing fibrin clot structure using a microplate reader. Blood Coagulation and Fibrinolysis. 13(6):533-9. PMID: 12192305

48. Rebekah White, Christopher Rusconi, Elizabeth Scardino, Alisa Wolberg, Jeffrey Lawson, Maureane Hoffman, Bruce Sullenger. 2001. Generation of species cross-reactive aptamers using “toggle” SELEX. Molecular Therapy. 4(6):567-73. PMID: 11735341

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49. Alisa S. Wolberg, Rachel H. Kon, Dougald M. Monroe, Mirella Ezban, Harold R. Roberts, Maureane Hoffman. 2000. De-encryption of cellular tissue factor is independent of its cytoplasmic domain. Biochemical and Biophysical Research Communications. 272(2):332-6. PMID: 10833414

50. Alisa S. Wolberg, Rachel H. Kon, Dougald M. Monroe, Maureane Hoffman. 2000. Factor XI is a contaminant in intravenous immunoglobulin preparations. American Journal of Hematology. 65(1):30-4. PMID: 10936860

51. Alisa S. Wolberg, Dougald M. Monroe, Harold Roberts, Maureane R. Hoffman. 1999. Tissue factor de-encryption: Ionophore treatment induces changes in tissue factor activity by phosphatidylserine-dependent and -independent mechanisms. Blood Coagulation and Fibrinolysis. 10(4):201-10. PMID: 10390120

52. Alisa S. Wolberg, Darrel W. Stafford and Dorothy A. Erie. 1997. Human factor IX binds to specific sites on the collagenous domain of collagen IV. Journal of Biological Chemistry. 272(27):16717-20. PMID: 9201971

53. Alisa S. Wolberg, Daniel P. Morris, Darrel W. Stafford. 1997. Activation of factor IX by factor XIa proceeds without release of a free intermediate. Biochemistry. 36(14):4074-9. PMID: 9100000

54. Alisa S. Wolberg, Leping Li, Wing-Fai Cheung, Nobuko Hamaguchi, Lee G. Pedersen, Darrel W. Stafford. 1996. Characterization of gamma-carboxyglutamic acid residue 21 of human factor IX. Biochemistry. 35(32):10321-7. PMID: 8756687

55. Wing-Fai Cheung, Alisa S. Wolberg, Darrel W. Stafford, Kenneth J. Smith. 1995. Localization of a metal-dependent epitope to the amino terminal residues 33-40 of human factor IX. Thrombosis Research. 80(5):419-27. PMID: 8588203

56. Cynthia A. Richards, Alisa S. Wolberg, Brian Huber. 1993. The transcriptional control region of the human carcinoembryonic antigen gene: DNA sequence and homology studies. DNA Sequence. 4(3):185-96. PMID: 8161821

*Corresponding author REFEREED, PUBLISHED ABSTRACTS, POSTERS, AND ORAL PRESENTATIONS

1. Maria M. Aleman, Jian-Guo Wang, Jay L. Degen, Matthew J. Flick, Alisa S. Wolberg. 2012. Fibrin(ogen)-leukocyte integrin receptor αMβ2 interactions in the control of venous thrombus formation and resolution. XXIInd International Fibrinogen Workshop, Brighton, UK, Oral presentation

2. J.G. Wang, J. Gambone, M.M. Aleman, J.C. Cardenas, P. Chantrathammachart, J.C. Williams, D. Kerchhofer, V.Y. Bogdanov, R.R. Bach, F.C. Church, A.S. Wolberg, R. Pawlinski, N.S. Key, J.J. Yeh, N. Mackman. 2012. Human pancreatic cancer cell tissue factor activates coagulation in a mouse model. International Conference on Thrombosis and Hemostasis Issues in Cancer, Bergamo, Italy, Poster presentation

3. Rita Marchi, Bethany L. Walton, Colleen S. McGary, Feng-Chang Lin, Alice D. Ma, Rafal Pawlinski, Nigel Mackman, Robert A. Campbell, Jorge Di Paola, Alisa S. Wolberg. 2012. Dysregulated coagulation from co-existing hypofibrinogenemia and elevated factor VIII. Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Chicago, IL, Poster #416.

4. Larissa Bornikova, Gary Brodsky, Keith B. Neeves, Rita Marchi, Donald S. Backos, Philip Reigan, PhD, Alisa S. Wolberg, Marilyn J. Manco-Johnson, Jorge Di Paola. 2011. A novel missense mutation in FGG (c.944C>A) encodes for an amino acid change (p.Ala315Asp) in the gamma chain of fibrinogen causing hypofibrinogenemia and a thrombotic phenotype. 53rd Meeting of the American Society of Hematology, San Diego, CA

5. Kellie R. Machlus, Alisa S. Wolberg. 2011. Causal relationship between hyperfibrinogenemia, thrombosis, and resistance to thrombolysis. XXIII ISTH Congress with 57th Annual SSC Meeting, Kyoto, Japan, Abstract #00009, Poster presentation

6. Kellie R. Machlus, Alisa S. Wolberg. 2011. Extent of vascular injury determines contribution of elevated factor VIII to thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Chicago, IL. Oral Presentation (62)

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7. Candice Bailey Smith, Maria M. Aleman, Joleen M. Soukup, John C. Lay, Alisa S. Wolberg, and Martha Sue Carraway. 2011. Exposure to air pollution enhances the generation of vascular microparticles. Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Chicago, IL. Poster Presentation (P-672)

8. Jennifer C. Carter, Robert A. Campbell, Jennifer A. Gibbons, Alisa S. Wolberg, Frank C. Church. 2009. Enhanced cell-associated fibrinolytic pathway but not coagulation pathway activity contributes to motility in metastatic breast cancer cells. 51st ASH Annual Meeting, Poster presentation (#3170) and oral presentation in the Special Symposium on the Basic Science of Hemostasis and Thrombosis

9. AS Wolberg, C Amin, S Adam, A Kutlar, B Hulkower, J-Y Chang, KI Ataga, NS Key. 2009. Thrombin generation and fibrin clot formation in patients with sickle cell disease (SCD). XXII ISTH Congress with 55th Annual SSC Meeting, Boston, MA, Abstract #PP-TH-655, Poster presentation, Ranked in top third of presented abstracts

10. Robert A. Campbell#, Katherine A. Overmyer, Alisa S. Wolberg. 2009. The procoagulant phenotype of the cell dictates fibrin clot structure. XXII ISTH Congress with 55th Annual SSC Meeting, Boston, MA, Abstract #OC-MO-073, Oral presentation, #Travel Award Recipient

11. Kellie R. Machlus, Emily A. Colby, Jogin R Wu, Gary G. Koch, Nigel S. Key, Alisa S. Wolberg. 2009. Use of calibrated automated thrombography to detect hypercoagulability. XXII ISTH Congress with 55th Annual SSC Meeting, Boston, MA, Abstract #PP-WE-299, Poster presentation

12. Veronique Ollivier, Jian-Guo Wang, David Manly, Kellie R. Machlus, Alisa S. Wolberg, Martine Jandrot-Perrus, Nigel Mackman. 2009. Detection of endogenous tissue factor levels in plasma using the calibrated automated thrombogram assay. XXII ISTH Congress with 55th Annual SSC Meeting, Boston, MA, Abstract #PP-TH-119, Poster presentation

13. Robert A. Campbell, Katherine A. Overmyer, Alisa S. Wolberg. 2009. The procoagulant phenotype of the cell determines fibrin clot structure. Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Washington D.C. Oral Presentation

14. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg. 2008. Cellular procoagulant activity dictates clot structure as a function of distance from the cell surface. Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Atlanta, GA, P186, Poster presentation

15. Robert A. Campbell, Thomas H. Fischer, Alisa S. Wolberg*. 2006. A novel use of reconstituted, lyophilized platelets increases recombinant FVIIa procoagulant activity in a model of hemophilia. 48th Meeting of the American Society of Hematology, Orlando, FL, Blood, 108(11):Abstract #882, Poster presentation

16. Alisa S. Wolberg*, Egon Persson, Mirella Ezban, Ulla Hedner, Geoffrey A. Allen. 2006. A variant of recombinant factor VIIa with enhanced procoagulant and antifibrinolytic activities in a model of hemophilia. 48th Meeting of the American Society of Hematology, Orlando, FL, Blood, 108(11):Abstract #767, Oral presentation

17. Robert A. Campbell, Thomas H. Fischer, Alisa S. Wolberg*. 2005. Rehydrated, lyophilized platelets generate thrombin in the presence of recombinant factor VIIa. 47th Meeting of the American Society of Hematology, Blood, 106(11): Abstract # 4057

18. Alisa S. Wolberg*, Mirella Ezban, Ulla Hedner, Egon Persson. 2005. Enhanced fibrin clot formation and stability with a superactive analog of factor VIIa in an in vitro model of hemophilia. 47th Meeting of the American Society of Hematology, Blood, 106(11): Abstract # 4056

19. Thomas H. Fischer, Alisa S. Wolberg, Arthur P. Bode, J. Kevin Ramer, Timothy C. Nichols. 2005. The interaction of factor VIIa with STASIXTM platelets. 47th Meeting of the American Society of Hematology, Blood, 106(11): Abstract #3994

20. Robert Campbell, Thomas Fischer, Alisa S. Wolberg*. 2005. Activity of recombinant factor VIIa (rFVIIa) on reconstituted, lyophilized platelets in an in vitro model of haemophilia. 8th Novo Nordisk Symposium on Haemostasis Management, May 11–13, 2005, Copenhagen, Denmark, Oral presentation

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21. Judson M. Englert, Jennifer L. Moen, Alisa S. Wolberg*. 2004. Altered fibrinopeptide release produces thicker fibrin fibers and an abnormal fibrin clot structure in an in vitro model of hemophilia B. International Fibrinogen Workshop, Poster presentation

22. Alisa S. Wolberg*, Dougald M. Monroe, Harold R. Roberts, Maureane Hoffman. 2004. High dose recombinant factor VIIa improves the fibrin clot structure of hemophilic clots in an in vitro model of hemophilia. International Fibrinogen Workshop, Oral presentation

23. Maureane Hoffman, Zhi Hong Meng, Alisa S. Wolberg, Dougald M. Monroe. 2003. A systematic evaluation of the effect of temperature on coagulation enzyme activity and platelet function. Journal of Thrombosis and Haemostasis 2003; 1 Supplement 1 July: Abstract #P0582, Poster presentation

24. Geoffrey Allen, Alisa S. Wolberg, Julie A. Oliver, Maureane Hoffman, Harold R. Roberts, Dougald M. Monroe. 2003. Impact of procoagulant concentration on rate, peak and total thrombin generation in a model system. Journal of Thrombosis and Haemostasis 2003; 1 Supplement 1 July: Abstract #P1270, Poster presentation

25. Maureane Hoffman, Zhi Hong Meng, Alisa S. Wolberg, Dougald M. Monroe. 2003. The effect of temperature and pH on the activity of FVIIa: implications for the efficacy of high-dose FVIIa in hypothermic and acidotic patients. Journal of Thrombosis and Haemostasis 2003; 1 Supplement 1 July: Abstract #P0592, Poster presentation

26. Alisa S. Wolberg*, Jennifer C. Carter, Caroline S. Chen, Robert A. S. Roubey. 2003. Anti-2 glycoprotein I antibodies do not increase TF activity by de-encryption. American College of Rheumatology, ACR/ARHP Annual Scientific Meeting, Poster presentation

27. Alisa S. Wolberg, Dougald M. Monroe, Ulla Hedner, Harold R. Roberts, Maureane Hoffman. 2001. High dose factor VIIa enhances clot stability in a model of hemophilia. 43rd Meeting of the American Society of Hematology. Abstract #3435, Oral presentation

28. Alisa S. Wolberg, Dougald M. Monroe, Harold R. Roberts, Maureane Hoffman. 2001. Elevated prothrombin results in clots with an altered fiber structure: a possible mechanism of the increased thrombotic risk. XVIIIth Congress of Thrombosis and Haemostasis. Abstract #OC166, Oral presentation

29. Alisa S. Wolberg, Don A. Gabriel, Maureane Hoffman. 2001. Analyzing fibrin clot structure using a microplate reader. XVIIIth Congress of Thrombosis and Haemostasis. Abstract #P2770

30. Derrick L. Sauls, Alisa S. Wolberg, Maureane Hoffman. 2001. Homocysteine-induced alterations in fibrinogen function and fibrin clot structure in vivo. 2nd Annual Conference on Arteriosclerosis, Thrombosis and Vascular Biology.

31. Derrick L. Sauls, Alisa S. Wolberg, Maureane Hoffman. 2001. Homocysteine-induced alterations in fibrinogen function and fibrin clot structure in rabbit and mouse models. XVIIIth Congress of Thrombosis and Haemostasis. Abstract #OC2516

32. Derrick L. Sauls, Alisa S. Wolberg, Maureane Hoffman. 2000. Hyperhomocysteinemia induces alterations in fibrinogen function and fibrin clot structure in a rabbit model. 42nd Meeting of the American Society of Hematology. Abstract #157

33. Alisa S. Wolberg, Rachel H. Kon, Dougald M. Monroe, Mirella Ezban, Harold R. Roberts, Maureane Hoffman. 1999. De-encryption of cellular tissue factor is independent of its cytoplasmic domain. 41st Meeting of the American Society of Hematology. Abstract # 1004, Poster presentation

34. Alisa S. Wolberg, Julie A. Oliver, Priscilla A. McDowell, Laura L. Phillips, Maria E. Mohan, Dougald M. Monroe, Harold R. Roberts, Maureane Hoffman. 1999. The initial rate, but not the total amount, of thrombin (IIa) generated determines fibrin clot structure in a cell-based model of coagulation. 41st Meeting of the American Society of Hematology. Abstract # 1019, Poster presentation

35. Rachel H. Kon, Alisa S. Wolberg, Julie A. Oliver, Dougald M. Monroe, Maureane Hoffman. 1999. Factor XI is a contaminant in intravenous immunoglobulin (IVIG) preparations. Thrombosis and Hemostasis Supplement: 459 and XVIIth ISTH Congress. Featured poster presentation

36. Geoffrey A. Allen, Alisa S. Wolberg, Julie A. Oliver, Maureane Hoffman, Harold R. Roberts, Dougald M. Monroe. 1999. Effect of varied procoagulant concentration on thrombin generation in a

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model system. Thrombosis and Hemostasis Supplement: 316 and XVIIth ISTH Congress, Poster presentation

37. Alisa S. Wolberg, Maureane Hoffman, Dougald M. Monroe, Harold R. Roberts. 1997. De-encryption of cellular tissue factor activity increases activation of both factors IX and X. 39th Meeting of the American Society of Hematology. San Diego, CA, Blood. 90: Poster presentation

38. Alisa S. Wolberg, Maureane Hoffman, Dougald M. Monroe and Harold R. Roberts. 1997. Competition between factors X and IX for activation by f.VIIa/tissue factor (TF): Effect on thrombin (IIa) production in a cell-based model. 39th Meeting of the American Society of Hematology, San Diego, CA, Blood. 90: 293a, Poster presentation

39. Alisa S. Wolberg, Daniel P. Morris, David L. Straight, Darrel W. Stafford. 1995. Activation of factor IX proceeds without release of a free FIX inte rme dia te . Blood. 86: 892a

40. Alisa S. Wolberg, Wing-Fai Cheung, Darrel W. Stafford, Nobuko Hamaguchi. 1993. Characterization of γ-carboxyglutamic acid residues 20 and 21 in human factor IX. XIVth ISTH Congress. New York, NY, Thrombosis and Haemostasis 69: 614, Poster presentation

UNREFEREED WORKS: PUBLISHED AND UNPUBLISHED ABSTRACTS, POSTERS, PAPERS, AND ORAL

PRESENTATIONS 1. Maria M. Aleman, Jian-Guo Wang, Jay L. Degen, Matthew J. Flick, Alisa S. Wolberg. 2012.

Fibrin(ogen)-leukocyte interactions orchestrate venous thrombus formation and resolution. Gordon Research Symposium on Hemostasis, Waterville Valley, NH, Oral presentation

2. Bethany L. Walton, Todd M Getz, Shirley Uitte de Willige, Wolfgang Bergmeier, Alisa S. Wolberg. 2012. Contribution of the fibrin(ogen) gamma prime chain to clot formation, structure, and stability. Gordon Research Conference on Hemostasis, Waterville Valley, NH, Poster presentation

3. Bethany L. Walton, Todd M Getz, Shirley Uitte de Willige, Wolfgang Bergmeier, Alisa S. Wolberg. 2012. Contribution of the fibrin(ogen) gamma prime chain to clot formation, structure, and stability. 6th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor: Hemostasis and Beyond, Chapel Hill, NC, Poster presentation

4. Maria M. Aleman, Jian-Guo Wang, Nigel Mackman, Jay L. Degen, Matthew J. Flick, Alisa S. Wolberg. 2012. Fibrin(ogen)-leukocyte interactions orchestrate venous thrombus formation and resolution. 6th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor: Hemostasis and Beyond, Chapel Hill, NC, Poster presentation

5. Rita Marchi, Bethany L. Walton, Colleen S. McGary, Feng-Chang Lin, Alice D. Ma, Rafal Pawlinski, Nigel Mackman, Robert A. Campbell, Jorge Di Paola, Alisa S. Wolberg. 2012. Dysregulated coagulation from co-existing hypofibrinogenemia and elevated factor VIII. 6th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor: Hemostasis and Beyond, Chapel Hill, NC, Poster presentation

6. Julia E. Geddings, Jian-Guo Wang, Maria M. Aleman, Jessica C. Cardenas, Pichika Chantrathammachart, Julie C. Williams, Daniel Kerchhofer, Vladimir Y. Bogdanov, Ronald R. Bach, Frank C. Church, Alisa S. Wolberg, Rafal Pawlinski, Nigel S. Key, Jen-Jen Yeh, and Nigel Mackman. 2012. Relationship between tissue factor expression by human pancreatic cancer cells and activation of coagulation and thrombosis in a mouse model. 6th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor: Hemostasis and Beyond, Chapel Hill, NC, Poster presentation

7. Bethany L. Walton, Todd M. Getz, Shirley Uitte de Willige, Wolfgang Bergmeier, Alisa S. Wolberg. 2012. Contribution of the fibrin(ogen) gamma prime chain to clot formation, structure, and stability. UNC Translational Medicine Symposium, Chapel Hill, NC, Winner, Best Poster Presentation

8. Maria M. Aleman, Jian-Guo Wang, Jay L. Degen, Alisa S. Wolberg. 2012. Fibrin(ogen)-leukocyte interactions orchestrate venous thrombus formation and resolution. McAllister Heart Institute/IVB Symposium, Chapel Hill, NC, Oral presentation

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9. Xiaomin Liu, Jian Mu, Kellie R. Machlus, Alisa S. Wolberg, Elliot D. Rosen, Zhiliang Xu, Mark S. Alber, Danny Z. Chen. 2012. Automatic segmentation and analysis of fibrin networks in 3D confocal microscopy images. Proceedings of SPIE Medical Imaging 2012.

10. Samantha Snow, Alisa S. Wolberg, Martha Sue Carraway. 2012. Soluble components of ultrafine particulate matter induce pro-coagulant responses in endothelial cells. UNC Annual Toxicology Retreat, Chapel Hill, NC. 2nd place winner, poster competition.

11. Matthew J. Heisler, Christa I. DeVette, Kellie R. Machlus, Maria M. Aleman, Alisa S. Wolberg. 2011. Fibrin(ogen) as a serine protein inhibitor in hyperfibrinogenemia. 6th International Serpins Symposium, Chapel Hill, NC

12. Maria M. Aleman, Matthew J. Heisler, Erin Lewchuk, Laura D. Gray, Jay L. Degen, Alisa S. Wolberg. 2011. Role of fibrin(ogen) in thrombus resolution. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

13. Bethany L. Walton, Matthew J. Heisler, Susan T. Lord, Alisa S. Wolberg. 2011. Delineating mechanisms by which hyperfibrinogenemia promotes thrombosis. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

14. Joseph Nuffer, Patrick Moseby, Michaael R. Falvo, Alisa S. Wolberg. 2011. A microfluidic device to investigate plasma shear stress and composition. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

15. Maria M. Aleman$, Chris Gardiner, Paul Harrison, Alisa S. Wolberg. 2011. Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability. McAllister Heart Institute/IVB Symposium, Chapel Hill, NC. Winner, Best Poster presentation

16. Christa I. DeVette, Alisa S. Wolberg. 2011. The roles of factor XIII, factor VIII, and fibrinogen in clot retraction. 6thAnnual ACC Meeting of the Minds Undergraduate Research Conference, Miami, FL

17. Gongting Wu, Alisa S. Wolberg, Amy L. Oldenburg. 2010. A novel way of measuring the mechanical property of fibrin clots by using resonance acoustic spectroscopy. North Carolina Biophysics Symposium, NC

18. Christa I. DeVette, Alisa S. Wolberg. 2010. The roles of factor XIII, factor VIII, and fibrinogen in clot retraction. State of North Carolina Undergraduate Research and Creativity Symposium, Raleigh, NC

19. Jessica C. Cardenas, Kellie R. Machlus, Alisa S. Wolberg, Frank C. Church. 2010. Inducing senescence in vascular endothelial cells promotes a prothrombotic phenotype. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

20. Maria M. Aleman, Sam Gover, Chris Gardiner, Paul Harrison, Raj Kasthuri, Alisa S. Wolberg. 2010. Procoagulant microparticles support thrombin generation and modulate fibrin formation. Gordon Research Conference on Hemostasis, Waterville Valley, NH

21. Kellie R. Machlus, Jessica C. Cardenas, Frank C. Church, Alisa S. Wolberg. 2010. Hypercoagulability and endothelial dysfunction as a two-hit mechanism for thrombosis. Gordon Research Conference on Hemostasis, Waterville Valley, NH

22. Jessica C. Cardenas, Kellie R. Machlus, Alisa S. Wolberg, Frank C. Church. 2010. Inducing senescence in vascular endothelial cells promotes a prothrombotic phenotype. Gordon Research Conference on Hemostasis, Waterville Valley, NH

23. Kellie R. Machlus, Alisa S. Wolberg. 2010. Separate and combined roles of endothelial activation and plasma procoagulant activity in fibrin network formation and structure. 5th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor: from Cell Biology to Animal Models, Chapel Hill, NC, *Poster presentation 2nd place winner

24. Kellie R. Machlus, Alisa S. Wolberg. 2009. Separate and combined roles of endothelial activation and plasma procoagulant activity in clot formation and structure. Annual Symposium of the

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Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

25. Maria M. Aleman, Sam Glover, Patrick Moody, Raj Kasthuri, Nigel S. Key, Nigel Mackman, Alisa S. Wolberg. 2009. Different effects of monocyte- and platelet-derived microparticles on thrombin generation. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

26. Kellie R. Machlus, Emily A. Colby, Gary G. Koch, Jogin R. Wu, Alisa S. Wolberg. 2009. Use of calibrated automated thrombography to detect hypercoagulability. The Aging Exchange: Education, Research and Service at the University of North Carolina at Chapel Hill, Chapel Hill, NC

27. Michael Gomez-Melendez$, Kellie R. Machlus, Jessica Cardenas, Hongmin Sun, Alisa S. Wolberg. 2009. The effect of reduced plasma factor V on clot structure. Annual Biomedical Research Conference for Minority Students (ABRCMS), Nov 4-7, 2009, Phoenix, AZ, $Winner, travel award

28. Michael Gomez-Melendez, Kellie R. Machlus, Jessica Cardenas, Hongmin Sun, Alisa S. Wolberg. 2009. The effect of reduced plasma factor V on clot structure. Southeastern Regional Meeting of the American Chemical Society, San Juan, Puerto Rico

29. Michael Gomez-Melendez, Kellie R. Machlus, Jessica Cardenas, Hongmin Sun, Alisa S. Wolberg. 2009. The effect of reduced plasma factor V on clot structure. UNC Annual Summer Undergraduate Research Symposium and Poster Session, Chapel Hill, NC

30. Feng Li, Chih-Hong Wang, Tiffany Thai, Gunnar Boysen, Longquan Xu, Alisa S. Wolberg, Nobuyo Maeda, Nobuyuki Takahashi. 2008. Elevated tissue factor activity and hypercoagulability in severe glomerulosclerosis of diabetic mice lacking eNOS. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

31. Kellie R. Machlus, Emily A. Colby, Gary G. Koch, Jogin R. Wu, Alisa S. Wolberg. 2008. Use of calibrated automated thrombography to detect a hypercoagulable phenotype. The Aging Exchange: Education, Research and Service at the University of North Carolina at Chapel Hill, Chapel Hill, NC

32. Kellie R. Machlus, Emily A. Colby, Gary G. Koch, Jogin R. Wu, Alisa S. Wolberg. 2008. Use of calibrated automated thrombography to detect a hypercoagulable phenotype. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

33. Robert A. Campbell, Kellie R. Machlus, Alisa S. Wolberg. 2008. The procoagulant response of the endothelium to inflammatory cytokines modulates fibrin clot structure. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

34. Robert A. Campbell, Kellie R. Machlus, Alisa S. Wolberg. 2008. The procoagulant response of the endothelium to inflammatory cytokines modulates fibrin clot structure. Gordon Research Conference on Hemostasis, Waterville Valley, NH

35. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg. 2008. Cell procoagulant activity dictates the three-dimensional structure of a fibrin clot. Gordon Research Conference on Hemostasis, Waterville Valley, NH

36. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg. 2008. The role of cellular procoagulant activity in clot formation. 9th Annual Women’s Health Research Day, Chapel Hill, NC

37. Laura Gray, Brittany Larson, Judson M. Englert, Robert A. Campbell, Mirella Ezban, Alisa S. Wolberg. 2008. Recombinant Factor VIIa Analog (V158D/E296V/M298Q-FVIIa, NN1731) Enhances Fibrin Formation, Structure and Stability in Hemophilic Plasma. 4th Symposium on Hemostasis with Special Focus on Factor VIIa & Tissue Factor – Understanding the Molecular Mechanism, Chapel Hill, NC

38. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg. 2008. Cell-mediated in situ thrombin generation dictates clot structure as a function of distance from the cell

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surface. 4th Symposium on Hemostasis with Special Focus on Factor VIIa & Tissue Factor – Understanding the Molecular Mechanism, Chapel Hill, NC, *Winner, best poster presentation

39. Laura Gray, Brittany Larson, Judson M. Englert, Robert A. Campbell, Mirella Ezban, Alisa S. Wolberg. 2008. Recombinant Factor VIIa Analog (V158D/E296V/M298Q-FVIIa, NN1731) Enhances Fibrin Formation, Structure and Stability in Hemophilic Plasma. Ninth Workshop on Novel Technologies and Gene Transfer for Hemophilia, Philadelphia, PA

40. Robert A. Campbell, Katherine A. Overmyer, C. Robert Bagnell Jr., Alisa S. Wolberg. 2007. Intercellular differences in procoagulant activity and its role in determining fibrin clot architecture. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, *Winner, Best Poster Presentation

41. Kellie R. Machlus, Robert A. Campbell, Alisa S. Wolberg. 2007. The effect of glycerol on thrombin generation and fibrin clot structure. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

42. Chih-Hong Wang, Feng Li, Tiffany Thai, Gunnar Boysen, Longquan Xu, Alisa Wolberg, Nobuyo Maeda, J. Charles Jennette, and Nobuyuki Takahashi. 2007. Severe glomerulosclerosis in diabetic mice lacking eNOS on a high fat diet. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

43. Chara May, Alisa S. Wolberg. 2007. Effect of lipid vesicles on fibrin clot structure in normal and hemophilic plasma. SPGRE poster session, Chapel Hill, NC

44. Katherine A. Overmyer, Robert A. Campbell, Alisa S. Wolberg. 2007. Role of TAFI in clot stability. Research in the Capital. April 17, 2007. Raleigh, North Carolina. Poster Presentation

45. Katherine A. Overmyer, Robert A. Campbell, Alisa S. Wolberg. 2007. Role of TAFI in clot stability. The 8th Annual Celebration of Undergraduate Research at UNC, Chapel Hill, NC

46. Robert A. Campbell, Alisa S. Wolberg. 2007. Cellular procoagulant activities dictate clot structure as a function of distance from the cell. Carolina Cardiovascular Biology Center, Vascular Biology Forum, Chapel Hill, NC

47. Lance Warren, Robert A. Campbell, Susan T. Lord, Alisa S. Wolberg. 2007. Lateral aggregation in BβA68T recombinant fibrinogen using a cell-based system. University of North Carolina Medical Student Research Day, Chapel Hill, NC

48. Patrice J. Gladden, Alisa S. Wolberg. 2006. Factor XI contaminates KKO monoclonal antibody. 2006. Annual Biomedical Research Conference for Minority Students (ABRCMS), November 8 – 11, 2006, Anaheim, California, Poster #D11

49. Robert A. Campbell, Thomas H. Fischer, Alisa S. Wolberg. 2006. Recombinant factor VIIa combined with reconstituted, lyophilized platelets increases thrombin generation and clot formation in a model of hemophilia. Annual Symposium of the Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

50. Patrice J. Gladden, Alisa S. Wolberg. 2006. Factor XI contaminates KKO monoclonal antibody. SPGRE poster session, Chapel Hill, NC

51. Robert A. Campbell, Thomas Fischer, Alisa S. Wolberg. 2006. Thrombin generation on reconstituted, lyophilized platelets in the presence of recombinant factor VIIa. Carolina Cardiovascular Biology Center, Vascular Biology Forum, Chapel Hill, NC

52. Robert A. Campbell, Thomas Fischer, Alisa S. Wolberg. 2005. Thrombin generation on rehydrated, lyophilized platelets in the presence of recombinant factor VIIa. Carolina Cardiovascular Biology Center, Vascular Biology Forum, Chapel Hill, NC

53. Judson M. Englert, Alisa S. Wolberg. 2005. Role of fibrinopeptide release in determining fibrin clot structure during in situ thrombin generation. Annual Celebration of Undergraduate Research, University of North Carolina, Chapel Hill, NC

54. Alisa S. Wolberg, H. Zhou, Robert A. S. Roubey. “Pathophysiology of the Antiphospholipid Syndrome,” Women’s Health Interdisciplinary Research Symposium, National Institutes of Health, Bethesda, Maryland, October 5, 2004

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55. Alisa S. Wolberg, Maureane Hoffman, Dougald M. Monroe, Harold R. Roberts. 1998. Cellular tissue factor activity: Contributions of de-encryption and phosphatidylserine exposure. Gordon Research Conference on Hemostasis

56. Alisa S. Wolberg, Darrel W. Stafford, Dorothy A. Erie. 1997. Human factor IX binds to specific sites on the collagenous domain of collagen IV. "Structural Insights into Biological Function" Symposium at the National Institute for Environmental Health Sciences, RTP, NC

57. Alisa S. Wolberg, Daniel P. Morris, Darrel W. Stafford. 1996. Determination of the rate constants for cleavage of factor IX a nd fa ctor IXaα by factor XIa. Gordon Research Conference on Hemostasis, Proctor Academy, NH

SUBMITTED MANUSCRIPTS AND MANUSCRIPTS IN PREPARATION Yesim Dargaud, Alisa S. Wolberg, Roger Luddington, Veronique Regnault, Henri Spronk, Trevor

Baglin, Thomas Lecompte Hugo TenCate Claude Negrier. Evaluation of a standardized protocol for thrombin generation measurement using the calibrated automated thrombogram: an international multicentre study. Submitted.

Candice B. Smith, Samantha J. Snow, Maria M. Aleman, Joleen M. Soukup, Alisa S. Wolberg, Martha S. Carraway. Exposure to diesel exhaust air pollution induces the generation of pro-inflammatory and pro-coagulant microparticles. Submitted.

Alexander Y. Mitrophanov, Alisa S. Wolberg, Jaques Reifman. Computational model captures principal kinetic features of thrombin generation, fibrin formation, and fibrinolysis in vitro. Submitted.

Chirag Amin, Soheir Adam, Abdullah Kutlar, Ferdane Kutlar, Denise Esserman, Kenneth I. Ataga, Jen-Yea Chang, Alisa S. Wolberg, Nigel S. Key. Activation of coagulation in sickle cell trait: possible role of circulating microparticle tissue factor. Manuscript in preparation.

Maria M. Aleman, Jian-Guo Wang, Jay L. Degen, Matthew Flick, Alisa S. Wolberg*. Role of fibrin(ogen) in thrombus resolution. Manuscript in preparation.

Bethany L. Walton, Todd M. Getz, Shirley Uitte de Willige, Wolfgang Bergmeier, Alisa S. Wolberg*. Role of the fibrinogen γ’ chain in arterial and venous thrombosis. Manuscript in preparation.

*Corresponding author TEACHING EXPERIENCE COURSE DIRECTORSHIP Pathology 713 “Mechanisms of Disease,” UNC at Chapel Hill Molecular and Cellular Pathology

Graduate Program, Co-Director: 8/2007 – present Science of Medicine (formerly “Integration Selection”), UNC at Chapel Hill School of Medicine, Co-

Director: 4/2011 – present PARTICIPATION IN COURSES FOR GRADUATE AND UNDERGRADUATE STUDENTS 5th Annual Carolina Workshop on Force Measurement and Manipulation in Biological Microscopy,

“Role of Surface-Driven Thrombin Generation on Fibrin Clot Structure and Stability,” Chapel Hill, North Carolina, 9/23/09 (1x1 hour lecture, ~12 students)

Biological and Biomedical Sciences Program 902 (First Year Group Seminar), UNC at Chapel Hill; Group Co-Mentor: 8/08 – 5/2009 (1.5 hrs/wk, Fall and Spring semesters, 16 students)

4th Annual Carolina Workshop on Force Measurement and Manipulation in Biological Microscopy, “Role of Surface-Driven Thrombin Generation on Fibrin Clot Structure and Stability” Chapel Hill, North Carolina, 5/28/08 (1x1 hour lecture, ~10 students)

Pathology 713 “Mechanisms of Disease,” UNC at Chapel Hill, lectures on Hemostasis and Thrombosis: 10/4-6/2006, 10/5-10/2007, 11/7-12/2008, 11/16-20/2009, 11/12-17/2010, 11/14-18/2011(3x1 hour lectures/year, ~25 students)

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Pathology 715 “Systemic Pathology,” UNC at Chapel Hill, lecture on thrombosis: 1/19/2007 (1x1 hour lecture, 21 students)

Teaching Assistant for Advanced Molecular Biology Laboratory at UNC at Chapel Hill, lectures on molecular modeling and molecular biology: 8/1992 – 5/1996

Biology 11L, UNC at Chapel Hill, Teaching Assistant for Introductory Biology Laboratory, labs on ecological and evolutionary relationships, biological diversity, and animal dissection: 8/1991 – 5/1992 (3 hour weekly labs, ~20 students)

GRAND ROUNDS (UNC AT CHAPEL HILL, CME) University of North Carolina, Department of Pathology and Laboratory Medicine Grand Rounds,

“Cellular and Soluble Contributions to Fibrin Formation, Structure, and Stability,” (CME Lecture) Chapel Hill, North Carolina, September 17, 2009

University of North Carolina, Department of Pathology and Laboratory Medicine Grand Rounds, “Thrombin Generation and Fibrin Clot Formation in Thrombosis and Bleeding,” (CME Lecture) Chapel Hill, North Carolina, August 17, 2006

University of North Carolina, Department of Pathology and Laboratory Medicine Grand Rounds, “Relationships between Thrombin Generation and Fibrin Clot Structure: Mechanisms of Thrombosis and Bleeding,” (CME Lecture) Chapel Hill, North Carolina. August 30, 2001

INVITED ORAL PRESENTATIONS (NATIONAL, INTERNATIONAL, CME)

AHA Council on Arteriosclerosis, Thrombosis, and Vascular Biology Annual Meeting, “Roles of factor VIII and fibrinogen in venous thrombosis,” Chicago, IL, April 18, 2012

University of Vermont, Department of Biochemistry, “Hypercoagulability in a Complex World,” Burlington, Vermont, February 24, 2012

Aflac Cancer Center Visiting Lecture Series and Emory University, “Hypercoagulability in a Complex World,” Atlanta, Georgia, November 17, 2011

North Carolina Biotechnology Center/Novo Nordisk, “Measuring Hemostatic and Thrombotic Properties: in vitro and in vivo models” Research Triangle Park, NC, August 26, 2011

XXIIIth Annual Meeting for the International Society on Thrombosis and Haemostasis, Scientific Subcommittee on Vascular Biology, “Differential Contributions of Monocyte- and Platelet-derived Microparticles towards Thrombin Generation and Fibrin Formation and Stability” Kyoto, Japan, July 23, 2011

University of Michigan, Department of Surgery, Section of Vascular Surgery, “Cellular and Soluble Contributions to Clot Formation, Structure and Stability” Ann Arbor, Michigan, January 31, 2011

Hemostasis, Bleeding and Therapeutic Approaches in 2010: New Therapies and New Paradigms IV, “Clot Formation, Structure and Stability: Virchow’s Triad Revisited” Emory University, Atlanta, CA, December 4, 2010

13th Biannual Midwest Platelet Conference, “Cellular and Soluble Contributions to Clot Formation, Structure, and Stability” Chapel Hill, NC, October 15, 2010

Gordon Research Conference on Hemostasis, “Plasma and Cellular Influences on Fibrin Structure and Stability” Waterville Valley, NH, July 27, 2010

1st Maastricht Summer School on Thrombin Generation and its Application, “Thrombin Generation Measurement and Fibrin Formation” Maastricht, Netherlands, June 17, 2010

5th Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor – from Cell Biology to Animal Models, “Modulation of Fibrin Structure by Cell Surface Tissue Factor” Chapel Hill, NC, April 30, 2010

10th Novo Nordisk Symposium on Haemostasis Management, “Understanding Fibrin Clot Strength and Stability” Prague, Czech Republic, October 1, 2009

Blood Research Institute, “Cellular and Soluble Contributions to Fibrin Formation, Structure, and Stability,” Milwaukee, Wisconsin, September 22, 2009

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University of Notre Dame, Center for Biocomplexity Colloquium, “Role of Surface-Driven Thrombin Generation on Fibrin Clot Structure and Stability” Notre Dame, Indiana, March 26, 2009

National Hemophilia Foundation’s 60th Annual Meeting, “Improving Recombinant Factor VIIa Treatment for Hemophilia,” (CME Lecture) Denver, CO, November 14, 2008

Mathematical Biology Seminar Series, University of Utah, “Role of Surface-Driven Thrombin Generation on Fibrin Clot Structure and Stability” Salt Lake City, Utah, March 26, 2008

Ninth Workshop on Novel Technologies and Gene Transfer for Hemophilia, “Novel Approaches to Improving Recombinant Factor VIIa Activity: Effects of a Preserved Platelet Preparation,” The Children’s Hospital of Philadelphia, Pennsylvania, February 23, 2008

Annual Meeting of the Southeastern Regional Hemophilia Treatment Centers, “Biochemical Effects of Bypass Therapy in Hemophilia Patients with Inhibitors,” (CME Lecture) Nashville, Tennessee, September 17, 2006

Eastern Canada Hemostasis/Hereditary Bleeding Disorders Meeting, “Overview of Hemostasis with a focus on Thrombin Generation and Clot Stability.” (CME Lecture) Old Montreal, Canada. September 14, 2006

3rd Symposium on Hemostasis with Special Focus on Factor VIIa and Tissue Factor, “Relationships between Thrombin Generation and Fibrin Clot Structure and Stability.” (CME Lecture) Chapel Hill, North Carolina. May 6, 2006

Bayer U.S. Hemophilia Advisory Board Meeting - Looking Towards Tomorrow, “Bypass versus Replacement Therapy for Hemophilia: Biochemical Effects of Recombinant Factor VIIa in Hemophilia B.” Miami, Florida. March 25, 2006

Novo Nordisk, “Thrombin Generation and Fibrin Clot Formation in Hemophilia: Effect of Factor VII(a) Variants.” Copenhagen, Denmark, May 10. 2005

Novo Nordisk. “Fibrin Clot Formation in Thrombosis and Bleeding: Insights from in vitro Models of Coagulation.” Copenhagen, Denmark, May 10, 2005

Center for Thrombosis and Hemostasis Symposium on Use of Recombinant Factor VIIa to By-Pass Inhibitors of Factors VIII and IX, “Effect of High Dose Factor VIIa on Clot Structure,” Chapel Hill, North Carolina. January, 2001

INVITED ORAL PRESENTATIONS (AT UNC AT CHAPEL HILL) UNC Biological & Biomedical Sciences Program Recruitment Weekend Keynote Address, “Complex

coagulopathy: probing a patient’s pathology using basic science," February 18, 2012 UNC Department of Pathology and Laboratory Medicine, Molecular and Cellular Pathology Annual

Research Symposium, “Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability,” September 30, 2011

UNC Biological & Biomedical Sciences Program Recruitment Weekend Keynote Address, “Elucidating Pathophysiologic Mechanisms in Thrombosis: an Interdisciplinary Approach," February 26, 2011

UNC Department of Physics, Computer Integrated Systems for Microscopy and Manipulation (CISMM) Site Visit, “Cell-, Plasma-, and Flow-Specific Determinants of Fibrin Clot Structure,” October 18, 2010

Symposium on Measurement of Microparticles (MPs) in Plasma and their Role in Disease, “MP in the CAT assay and fibrin formation,” April 29, 2010

UNC Institute on Aging, “Risk Factors for Venous Thromboembolism (VTE) in the Young and Elderly,” December 16, 2008

UNC Department of Physics, Computer Integrated Systems for Microscopy and Manipulation (CISMM) External Advisory Board Meeting, “Fibrin Formation during in situ Thrombin Generation,” October 10, 2007

UNC Department of Pathology and Laboratory Medicine Chalk Talk, Chapel Hill, North Carolina. August 11, 2005

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5th Annual Women’s Health Research Day, Center for Women’s Health Research, “Pathophysiology of the Antiphospholipid Syndrome,” Chapel Hill, North Carolina. March 17, 2004

UNC Biological Division, Department of Chemistry, “Hemophilia and Thrombosis: Studies Using an in vitro Model of Coagulation,” Chapel Hill, North Carolina. October 29, 2003

RESEARCH MENTORING TRAINEES (POSTDOCTORAL)

Trainee Period Training Position

Research Project(s) Present Position (past trainees)/

Source of Support (current trainees)

Jian-Guo Wang, PhD 2/12–-pres Postdoc Leukocytes in Thrombus Resolution R01HL094740 Joseph H. Nuffer, PhD 6/11–11/11 Postdoc Role of Shear in Thrombosis Unknown Robert A. Campbell, PhD

5/09–7/09 Postdoc Vascular Cells and Clot Structure Postdoc, U Utah

TRAINEES (GRADUATE AND MEDICAL STUDENTS)




Bethany L. Walton 8/10–pres Predoc Hypercoagulability and Thrombosis HHMI Translational Medicine Program, IVB Training Program

Maria M. Aleman 8/08–pres Predoc Microparticles in Thrombosis NIH T32ES007017, AHA Predoc

Kellie R. Machlus, PhD 5/06–9/11 Predoc Hypercoagulability and Thrombosis Postdoc, Harvard Robert A. Campbell, PhD

8/04–5/09 Predoc Vascular Cells and Clot Structure Postdoc, U Utah

TRAINEES (UNDERGRADUATES AND HIGH SCHOOL STUDENTS)




Alice Xu 5/12-pres Undergrad Role of fibrinogen in thrombosis Undergrad, UNC Matthew J. Heisler 5/11-12/11 Undergrad Plasma Activity in Thrombosis Undergrad,

Northeastern U Erin Lewchuk 5/11-pres Undergrad Leukocytes in Thrombus Resolution HHMI, UNC Christa I. Devette 5/09-5/11 Undergrad Role of factor XIII in clot retraction MD/PhD Student,

U Utah Colleen S. McGary 8/08-5/10 Undergrad Procoagulant Activity and Fibrin

Formation Grad Student, Emory

Michael Gomez Melendez

5/09-7/09 Undergrad (RISE)

Fibrin organization in thrombosis Undergrad, U Puerto Rica, Cayey

Brittany M. Larson 8/07-5/09 Undergrad RFVIIa variants and clot formation MD student, Wake Forrest Univ

Katie A. Overmyer 8/05-7/08 Undergrad (Biol 98)

Role of Procoagulant Activity in Fibrin Formation

Grad Student, U Mich

Mark Steuerwald 1/08-4/08 Undergrad Protein expression on HUVEC Unknown Michael Platter 5/07-8/07 Undergrad Use of ARFI in Determining Clot

Stability Unknown

Chara L. May 5/07-7/07 Undergrad (CRUISE)

Effects of Large Unilamellar Vesicles on Clot Formation in Normal and Hemophilic Plasma

Unknown

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Patrice Gladden 5/06-7/06 Undergrad (SPGRE)

Factor XI contaminates KKO monoclonal antibody

Unknown

Marlon Sequeira 5/06-8/06 Undergrad (UCIS)

Investigation of the Rabies Epidemic in Kerala, India

DVM student, Cornell

Dax Varkey 1/05-12/06 Undergrad (Biol 98)

Interactions between Annexin A2 and B2GPI

Resident, UNC

Judson M. Englert, MD PhD

1/04-5/05 Undergrad (Chem 99)

Fibrinopeptide release in hemophilia B

Resident, Brigham and Women’s

Amit Bhagwandass 5/05-8/05 Undergrad (Biol 98)

Hemodilution and Clot Formation Unknown

Andreas M. Staebler, MD

5/03-8/04 Undergrad (Biol 98)

Factor XIII in Clot Formation Physician, Burlington, NC

Caroline S. Chen 5/02-8/02 Ugrad B2GPI and Tissue Factor Unknown

Rachel H. Kon, MD 6/98-8/02 Ugrad (HH Precol Prog)

Factor XI, Tissue Factor in Coagulation

Assistant Professor, Georgetown

Brett C. Niblack, MD 1/94-5/95 Ugrad (Biol 98)

Gla Residues in Clotting Factor IX Physician, Shelby, NC

TRAINEES (STUDENT THESIS COMMITTEES)

Trainee Period Department, Institution

Thesis Advisor

Degree, Year Completed, Research

Jessica Cardenas 1/10-5/12 Pathology, UNC FC Church PhD, 2012, VTE and Aging Samantha Snow 11/09-

pres Toxicology,UNC MS Carraway

Rupan Sandhu, PhD 1/08–8/11 Pathology, UNC WB Coleman PhD, 2011, Epigenetics of breast cancer

Ricky Spero, PhD 1/08–5/10 Physics, UNC R Superfine PhD, 2010, Novel technologies for measuring clot quality

Karin Leiderman Gregg, PhD

3/08–5/10 Math, U Utah A Fogelson PhD, 2010, Mathematical simulations of blood clotting under flow

Jennifer Carter, PhD 9/06–5/09 Pathology, UNC FC Church PhD, 2009, PAI-1, PPARγ, breast cancer

Jacqueline (Rischer) Roll, PhD

8/06–5/08 Pathology, UNC WB Coleman PhD, 2008, Epigenetics, breast cancer

Jill Rau, PhD 9/05–4/08 Pathology, UNC FC Church PhD, 2008, Serpin biology TRAINEES (ROTATION STUDENTS AND MEDICAL STUDENTS)


Research Project(s)

Leslie Kennedy 3/11–5/11 Predoc Role of platelet aggregation and fibrin polymerization in thrombosis

Jessica Cardenas 1/08–3/08 Predoc Role of Platelets in RFVIIa Efficacy Christianna Stack 3/07–5/07 Predoc Determining Clot Stability with ARFI Olguitza Guzman 3/07–5/07 Predoc RFVIIa in Canine Hemophilia Sybil Yeboah 9/06–12/06 Predoc Fibrinogen Effect on Clot Structure Rupninder Sandhu 8/06–12/06 Predoc Annexin A2 and B2GPI Interactions Lance A. Warren, MD 5/06–8/06 MD student Lateral aggregation in BβA68T recombinant fibrinogen Mark Gramling, PhD 8/05–12/05 Predoc HIT/T Antibodies in Platelet Activity

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CURRENT GRANT SUPPORT NIH/NHLBI (1R01HL094740-01A109), “Cellular Determinants of Fibrin Structure and Stability.”

09/10/2009 – 04/30/2014. Role: PI (40% FTE), $225,000/yr direct costs NIH/NHLBI (R21HL109832) “Novel device for sensitive, small sample volume assessment of clot

elasticity.” 07/01/2011 – 06/30/2013. PI: Oldenburg, Role: Co-I (10% FTE), $225,000 total direct costs

American Heart Association (12GRNT11840006), “Pathophysiology of hypercoagulability and thrombosis.” 07/01/2011 – 06/30/2013. PI (10% FTE), $70,000/yr direct costs

Novo Nordisk (No Number Assigned) “Influence of NovoSeven and NN1731 on Fibrin Deposition in an in vivo Model of Hemophilia” 01/24/12 – 01/23/13. Role: PI (15% FTE), $119,500/yr direct costs

Inspiration (No Number Assigned) “Inspiration Contract” 2/21/12 – 2/20/13. Role: PI (15% FTE), $101,700/yr direct costs

PAST GRANT SUPPORT: EXTRAMURAL North Carolina Biotechnology Center (2010-BES-0239), Event Sponsorship Grant for Program

“Measurement of Microparticles (MPs) in Plasma and their Role in Disease.” 04/28/2010 – 04/29/2010. PI (0% FTE) $1070 direct costs

American Heart Association Grant-In-Aid (09GRNT2250674) “Vascular Bed-Specific Determinants of Fibrin Structure and Stability.” 07/01/09 – 09/01/10. PI (15% FTE) $70,000/yr direct costs, Resigned due to scientific overlap with NIH award

Gustavus and Louise Pfeiffer Research Foundation, “Abnormal Clot Formation in Cardiovascular Disease.” 05/01/09 – 04/30/10. Role: PI (15% FTE), $72,000/yr direct costs

Novo Nordisk, “Relationships between Thrombin Generation and Fibrin Clot Structure in Hemophilia and Recombinant Factor VIIa Therapy” 06/01/08 – 05/31/10. PI (5% FTE), $92,486 direct costs

Gustavus and Louise Pfeiffer Research Foundation, “Abnormal Clot Formation in Cardiovascular Disease.” 05/01/08 – 04/30/09. Role: PI (5% FTE) $62,000/yr direct costs

National Hemophilia Foundation Career Development Award, “Recombinant Factor VIIa and RL Platelets as a Hemophilia Therapy” 07/01/07 – 06/30/10. PI (25% FTE), $64,815/yr direct costs

Gustavus and Louise Pfeiffer Research Foundation, “Abnormal Clot Formation in Cardiovascular Disease.” 12/01/06 – 11/30/07. Role: PI (10% FTE) $60,000/yr direct costs

Novo Nordisk, “Relationships between Thrombin Generation and Fibrin Clot Structure in Hemophilia and Recombinant Factor VIIa Therapy.” 11/28/06 – 12/31/08. Role: PI (3% FTE), $91,061 direct costs

NIH/NIAMS (1K01AR051021) “Pathology of Anti-B2GPI Antibodies in APS.” 7/1/2004 – 6/30/2009, Role: PI (88% FTE), $93,183/yr direct costs

Novo Nordisk, “Influence of Prothrombin and High Dose Activated Factor VII on Fibrin Clot Structure and Stability.” 11/10/03 – 10/31/06. Role: PI, $63,000 direct costs

Bayer Hemophilia Early Career Development Award, “Fibrin Clot Stability: Efficacy of Replacement vs Bypass Therapy in a Preclinical Hemophilia Model.” 07/01/03 – 06/30/05. Role: PI, (50% FTE) $68,729/yr direct costs, 2 years

NICHD-NIH (K12 HD01441) “Building Interdisciplinary Research Careers in Women’s Health (BIRCWH).” 7/01/00 - 6/30/10, PI: Orringer, $500,000, Role: Scholar, 2003 - 2004

American Heart Association Beginning Grant-In-Aid (0160418U), “Altered Clot Structure as a Mechanism for Thrombosis.” Role: PI. 07/01/01 – 06/30/03. Role: PI, (50% FTE) $66,000/yr direct costs, 2 years

National Research Service Award, National Institute of Health (1F32HL09978-01), “Cellular Tissue Factor: Regulation and Activity.” Role: PI. 07/01/98 – 06/30/01. Role: PI, (100% FTE) 3 years

American Heart Association Post-doctoral Fellowship (F98204N), “Cellular Tissue Factor: Regulation, Activity and the Initiation of Coagulation.” 1998. Declined in order to accept NIH/NRSA.

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PAST GRANT SUPPORT: INTRAMURAL NC TraCS Institute (10KR81034), “Hyperfibrinogenemia in a Novel Model of Thrombus Stability.”

12/01/2010 – 11/30/2011. Role: PI (0% FTE), $10,000/yr direct costs UNC Institute on Aging, Research Stimulus Grants in Aging Program, “Clot Structure and Venous

Thrombosis and Thromboembolism in the Elderly.” 07/01/07 – 06/30/09. Role: PI (0% FTE), $18,504 direct costs

UNC University Research Council Grant, “Mechanisms of Thrombin Generation in Heparin-Induced Thrombocytopenia.” 12/01/05 – 11/30/07, Role: PI (0% FTE), $4,000

UNC Medical Alumni Endowment Fund Grant, “Evaluation of a Recombinant Factor VIIa Variant (DVQ) as a Novel Anti-Bleeding Therapy for Hemophilia.” 06/01/05 – 05/31/06, Role: PI, $8,000

UNC Junior Faculty Development Award, “Activity of Recombinant Factor VIIa Conjugated to Rehydrated, Lyophilized Platelets: Evaluation Using an in vitro Model of Coagulation.” 01/01/04 – 12/31/04. Role: PI, $5,000

Durham Veterans Affairs Medical Center, Institute for Medical Research In – House Grant, “The Effect of Homocysteine on Clot Structure: a Direct Mechanism for Increasing Thrombotic Risk in Cardiovascular Disease.” 01/01/00 – 12/31/00. Role: PI, $15,000

PROFESSIONAL SERVICE: STATE/NATIONAL/INTERNATIONAL OFFICES, COMMITTEES and

APPOINTMENTS Member, Advisory Board, XXIV Congress of the ISTH, 2013 Session Chair, 2013 FASEB meeting on Proteases in Hemostasis and Vascular Biology: June, 2013 Coordinating Reviewer, 54th Annual Meeting of the American Society of Hematology: 2012 Session Moderator and Abstracts Reviewer, XXII International Fibrinogen Workshop, Brighton, UK:

July, 2012 Co-Chair, 6th Symposium on Hemostasis with Special Focus on Tissue Factor, Factor VIIa, and Tissue

Factor Pathway Inhibitor: Hemostasis and Beyond: Chapel Hill, NC, May 3-5, 2012 Session Co-Moderator, “Blood coagulation and fibrinolytic factors: control of thrombin generation,”

Annual Meeting of the American Society of Hematology: 2011 Abstracts Reviewer, 53rd Annual Meeting of the American Society of Hematology: 2011 Member, Steering Committee, Workshop on IGIV and Thrombosis, “Risk Mitigation Strategies to

Address Potential Procoagulant Activity in Immune Globulin Products,” FDA, Center for Biologic Evaluation and Research, Office of Blood Research and Review: 2011

Abstracts Reviewer, Arteriosclerosis, Thrombosis and Vascular Biology Annual Meeting: 2011 Ad hoc Reviewer, NIH Special Emphasis Panel/Scientific Review Group 2011/05 HLBP S: 2011 Member, American Society for Hematology (ASH) Scientific Subcommittee on Thrombosis and

Vascular Biology: January 1, 2011 – December 31, 2016 Member, Brinkhous Young Investigator Award Committee, American Heart Association: July 1, 2010 –

June 30, 2012 Session Co-Moderator, 1st Maastricht Summer School on Thrombin Generation and its Application

“Masterclass Session” and “Novel and Parallel Techniques”: June, 2010 Co-Organizer and Session Chair, Symposium on Measurement of Microparticles (MPs) in Plasma and

their Role in Disease, Chapel Hill, NC: April 29, 2010 Ad hoc Reviewer, Department of Defense FY10 Wound Infection and Healing (WIH) Peer Review

Panel of the Defense Medical Research and Development Program (DMRDP): 2010 Member, Working Party on Thrombin Generation Testing, FVIII/IX Subcommittee of the Scientific

Subcommittee of the International Society on Thrombosis and Hemostasis: 2009 – present Ad hoc Reviewer, NIH/NHLBI P30 proposals in Biomedical Research Core Centers: 2009

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Ad hoc Reviewer, NIH/NHLBI RC1 proposals in Vascular and Hematology [ZRG1 VH-D (58)]: 2009 Abstracts Reviewer, Arteriosclerosis, Thrombosis and Vascular Biology Annual Meeting: 2009 Session Co-Moderator, “Pathogenesis and Treatment of Hemophilic Inhibitors,” Annual Meeting of the

American Society of Hematology: 2008 Abstracts Reviewer, Annual Meeting of the American Society of Hematology: 2008 Grants Reviewer, American Heart Association National Center, Thrombosis/Throm BSCT2 Study

Section: 2006 – present Invited Peer Reviewer for International Grants Programs: K. U. Leuven Research Council, Israeli

Science Foundation, U.K. Medical Research Council PROFESSIONAL SERVICE: EDITORIAL POSITIONS

Co-Editor, Thrombosis Research Supplemental Issue for 6th Symposium on Hemostasis with Special Focus on Tissue Factor, Factor VIIa, and Tissue Factor Pathway Inhibitor: Hemostasis and Beyond: 2012

Associate Editor, Arteriosclerosis, Thrombosis, and Vascular Biology Special Issue on Venous Thromboembolism: 2011

Member, Advisory Board, Journal of Thrombosis and Haemostasis: 2011 – present Member, Editorial Board, Arteriosclerosis, Thrombosis, and Vascular Biology: July 1, 2010 – June 30,

2012 Ad hoc Manuscript Reviewer: Arteriosclerosis Thrombosis and Vascular Biology, Biological Research

for Nursing, Biomacromolecules, Blood, Blood Cells Molecules and Diseases, British Journal of Haematology, Cell Biochemistry and Biophysics, Cell Research, Expert Opinion on Biological Therapy, Expert Reviews in Molecular Medicine, Journal of Cardiovascular Pharmacology, Journal of Cellular and Molecular Medicine, Journal of Molecular and Cellular Cardiology, Journal of Thrombosis and Thrombolysis, Journal of Visualized Experiments, Metabolism, Proceedings of the National Academy of Sciences U. S. A., Thrombosis and Haemostasis, Thrombosis Research, Transfusion

PROFESSIONAL SERVICE: UNC at CHAPEL HILL Member, Faculty Search Committee, McAllister Heart Institute: 2011 Member, Search Committee for Pathology and Laboratory Medicine: 2011 Member, UNC School of Medicine Strategic Planning Task Force #7: 2011 – 2012 Mentor, Howard Hughes Medical Institute Future Scientists and Clinicians (HHMI-FSC) program: 2011

– present Member, Biological and Biomedical Sciences Program (BBSP) Pathogenesis Admissions Committee:

2010 – 2012 Member, Liaison Committee on Medical Education (LCME) Steering Committee: 2010 – 2012 Mentor, UNC Medical Student in Aging Research (MSTAR) Program: 2010 – present Core Faculty, UNC Program in Translational Medicine: 2009 – present Chairperson, Preliminary Exam committee, Molecular and Cellular Pathology Graduate Program,

Department of Pathology and Laboratory Medicine: 2008 – 2010 Member, Executive Committee, Molecular and Cellular Pathology Graduate Program: 2008 – 2010 Member, Education Committee, Molecular and Cellular Pathology Graduate Program: 2008 – 2010 Member, Preliminary Exam Committee, Molecular and Cellular Pathology Graduate Program,

Department of Pathology and Laboratory Medicine: 2008 Member, UNC Thrombosis and Hemostasis Program Seminar Series Organizing Committee: 2007 –

present Faculty Advisor, Marc J. Mass Memorial Lecture Series Steering Committee: 2005 – present

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Chairperson, Molecular and Cellular Pathology Annual Research Symposium: 2005, 2006 Organizer, Cardiovascular Biology, Hemostasis and Thrombosis Chalk Talks, Department of

Pathology and Laboratory Medicine: 2005 Member, Admissions Committee, Molecular and Cellular Pathology Graduate Program, Department of

Pathology and Laboratory Medicine: 2004 – 2007 PROFESSIONAL ASSOCIATIONS American Society of Hematology: 2006 – present International Fibrinogen Research Society: 2004 – present McAllister Heart Institute (formerly Carolina Cardiovascular Biology Center), University of North

Carolina at Chapel Hill: 2003 – present Center for Thrombosis and Hemostasis, University of North Carolina at Chapel Hill: 2002 – present American Heart Association Scientific Council: Arteriosclerosis, Thrombosis and Vascular Biology:

2001 – present International Society on Thrombosis and Haemostasis: 2000 – present

REFLECTIVE STATEMENT RESEARCH PHILOSOPHY: “Abnormal blood clotting is the primary cause of morbidity and mortality in the Western World.” – American Heart Association My research goals are to understand biochemical and cellular mechanisms governing hemostasis and thrombosis. Briefly, exposure of cellular tissue factor to blood triggers a series of enzymatic reactions culminating in thrombin generation. Thrombin activates platelets and cleaves the plasma protein, fibrinogen, to fibrin, which polymerizes into a web-like mesh to reinforce the blood clot. Evidence from epidemiological and basic research studies suggests the quality of the blood clot determines its ability to support hemostasis or trigger thrombosis. Over the past twenty years, I have investigated multiple aspects of coagulation, including contributions from tissue factor activity, thrombin generation, platelet activation and fibrin formation. My studies have led to exciting findings on mechanisms contributing to stable and unstable clot formation. I have shown that elevated rates of thrombin generation, such as those seen in the thrombotic disorder hyperprothrombinemia, produce clots with dense networks of thin fibrin fibers, whereas deficiency in factors VIII or IX, proteins missing in patients with hemophilia, causes the formation of clots that are weak, poorly-formed, and highly susceptible to fibrinolysis. I have shown that cellular procoagulant (tissue factor) activity dictates fibrin network structure and stability as a function of distance from the cell surface. I have extended these studies to show that soluble and cellular effects on fibrin network formation shorten the time to vessel occlusion in in vivo models of thrombosis, suggesting abnormal fibrin structure and stability is a fundamental pathophysiological mechanism contributing to hemostatic and thrombotic disorders. Through these studies, I have accumulated a cutting-edge array of in vitro, ex vivo, and in vivo technologies for investigating mechanisms in coagulopathy, with particular strengths in protein biochemistry, enzyme kinetics, cell biology, and microscopy. Using these coordinated approaches enables me to precisely control levels and types of plasma proteins and cells, and correlate enzyme activity (thrombin) and functional effect (clot formation) in vitro and in vivo. Together, my scientific findings and technical expertise have allowed me to assemble a strong research program that makes me poised to address novel, important questions on mechanisms leading to blood clot formation. ‘Now that you have the knowledge, what are you going to do with it?’ – Gertrude B. Elion, Nobel Laureate

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Presently, and over the next five to ten years, I will investigate aspects of coagulation stemming from recent findings on cellular, biochemical, and biophysical mechanisms operant in coagulation. For example, I have shown that cellular tissue factor activity is a central mechanism dictating fibrin network density. Since certain cells release tissue factor in the form of cellular blebs (microparticles) in response to bacterial endotoxin, exposure to environmental pollution, and chemotherapy, I am investigating the role(s) of tissue factor-bearing microparticles and microparticle procoagulant activity in fibrin network formation, structure, and stability. Since cellular integrins bind fibrin, I am investigating how interactions between fibrin and specific integrin-expressing cells govern thrombus formation and resolution. Recent physical and mathematical studies have shown that blood flow (shear) has dual roles in clotting because it both replenishes procoagulant reactants and removes coagulation products from the site of clotting. I am therefore conducting studies to investigate the role(s) of blood flow in fibrin network formation, structure and stability in different vascular regions, and how these processes contribute to hemostatic and thrombotic clot formation. Since epidemiologic studies show abnormalities in plasma or cellular function, alone, do not cause thrombosis ipso facto, I am investigating how multiple “hits” from abnormalities in plasma, cells, and flow interact to promote thrombosis in vivo. I anticipate these studies will shed light on clinical challenges related to predicting bleeding and thrombotic risk in patient populations and understanding mechanisms leading to embolism following thrombus formation. To conduct these studies, I have initiated exciting collaborations with local, national and international scientists in multidisciplinary areas, including medicine, surgery, physics, mathematics, and computer science. These collaborations will enable me to undertake basic, clinical, and translational studies examining pathologic mechanisms operant in patients with hemophilia, sickle cell disease, dysfibrinogenemia, cancer, and other coagulopathies. These collaborations will also lead to the development of new tools for analyzing fibrin network characteristics in micrographs captured using confocal microscopy, and high-throughput analysis of clot biochemical and mechanical properties. Together, I anticipate these studies will elucidate coagulation mechanisms, and novel approaches to predict, identify, and treat bleeding and clotting disorders. In sum, my research program is built upon twenty years of experience studying mechanisms contributing to hemostasis and thrombosis. My scientific methodologies have demonstrated their utility as important tools for both basic and preclinical studies. Because abnormal blood clotting is the primary cause of morbidity and mortality in the Western World, my work has strong applicability to a broad spectrum of clinical presentations. I anticipate this strength will manifest as a diverse source of potential funding sources, including federal agencies (National Institutes of Health and the Department of Defense), private foundations (American Heart Association, National Diabetes Foundation) and industry. Given these strengths, I am well-positioned to lead an innovative and dynamic research program, and look forward to a long and productive academic research career. TEACHING PHILOSOPHY:

1) Involve students in the excitement of discovery, lead them towards developing and testing new concepts, and teach them how to communicate results and ideas to the scientific and medical community

2) Excite and involve laypersons in scientific inquiry and the support of basic research, through conversation and formal and informal presentations.

My academic teaching efforts are divided into two main avenues: classroom teaching and mentoring and laboratory training of undergraduate and graduate students. I also have administrative roles in the Molecular and Cellular Pathology Ph.D. education program in the UNC School of Medicine. Classroom Teaching 1991 – 1996: While enrolled in my Ph.D. program, I taught two semesters (2 sections/semester, ~20 students/section) of Introductory Biology Laboratory for the UNC Biology program. This course was organized by Dr. Jean DeSaix. I prepared the laboratory, gave introductory lectures on scientific methods, ecological and evolutionary relationships, biological diversity, and animal dissection, and constructed and graded exams. I also taught individual lectures in an Advanced Molecular Biology Laboratory course directed by Dr. Darrel Stafford (UNC Dept of Biology). I instructed students in bacterial transformations and gave introductory lectures on molecular modeling of protein structures.

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2008 – present: I Co-Direct Pathology 713 (Molecular and Cellular Pathophysiology of Disease: Mechanisms of Disease), a graduate course on cell injury and pathogenesis of disease with emphasis on basic mechanisms at the molecular, cellular and organismal levels. This course is offered in the fall semesters and dovetails with a laboratory course with a similar topic outline. The lecture portion enrolls 20 – 30 graduate students. Pathology 713 is team-taught, whereby my co-Director, Dr. Jonathon Homeister, and I coordinate members of the Pathology and Laboratory Medicine faculty and guest lecturers whom present information on histology and pathologic mechanisms of disease. The course content is derived from the leading pathology textbook and primary literature. Our objective is to provide graduate-level students with an exposure to histology and pathology and a foundation for conducting basic research in pathology. I teach three lectures on Hemostasis and Thrombosis in Pathology 713 that are quite well-received by students. I also design and grade exams on this lecture content. Prior to directing Pathology 713, I taught lectures on Hemostasis and Thrombosis in this course, as well as in its Spring-semester counterpart, Pathology 715 (Molecular and Cellular Pathophysiology of Disease: Systemic Pathology). Student evaluations of these lectures are included as supporting documents in my Teaching Portfolio. I Co-Direct CC34 Science of Medicine, a course designed to re-emphasize the basic sciences in the senior year of medical school. The ultimate goal of the course is to provide students with an opportunity to delve more deeply into the basic science of clinical problems than what was encountered in the third year clerkships, re-emphasize basic science from preclinical years and provide a foundation for evidence-based practice of clinical medicine. Mentoring and Laboratory Training of Undergraduate and Graduate Students Of all the teaching activities in which I engage, I have found the most gratification working with students one-on-one in the laboratory. I am currently training two Ph.D. students enrolled in the Molecular and Cellular Pathology Graduate Program. I also routinely mentor UNC undergraduates and non-UNC undergraduates through summer minority enrichment programs hosted by UNC-Chapel Hill. I see my support of the educational, scientific, and personal development of my trainees as the single most important commitment I have made as an academic researcher. I endeavor to create a supportive, caring, and productive environment for my trainees and laboratory personnel. I have an “always open-door” policy and I interact and discuss science and professional development with each trainee every day. I am continuously surprised to find that things I learn as a parent of two amazing children enlighten the way that I mentor my students, and vice-versa. Like my children, my students are unique individuals. Each student learns and asks questions in different ways. Each develops differently, with unique talents and interests. I strive to tailor my educational style to the learning needs of each student, to reinforce each student’s strengths and abilities, and to bolster each student’s self-confidence as they become independent and productive scientists. The pride I feel in watching my students succeed is that of a mentor, a colleague, and a friend. I have had the honor of seeing my first two graduate students defend their theses, choose post-doctoral positions, and leave my lab prepared to begin the next phase of their scientific careers. Several previous undergraduate students have also gone on to post-graduate scientific education following their work in my laboratory. These students have expressed tremendous enthusiasm for their training opportunities in my lab, and several have written letters describing their experiences in my lab. These letters are included as supporting documents in my Teaching Portfolio. I derive enormous satisfaction from having contributed to the futures of these talented individuals and am grateful for the opportunity to have influenced their lives so positively. In addition to students that I directly mentor, I have also served on dissertation committees for Ph.D. students in other Departments at UNC-Chapel Hill and other institutions. I find this activity immensely rewarding because it exposes me to students and research conducted somewhat outside of my primary focus, and enriches and broadens the educational and research experiences that I bring back to my lab. Administrative Activities in the Graduate Program In 2005 and 2006, I organized the Annual Research Symposium of the Program in Molecular and Cellular Pathology in the UNC School of Medicine. This event highlights work being conducted by graduate students and postdoctoral fellows, and reflects the diversity of ongoing research in our department. The afternoon begins with oral presentations by selected students and postdoctoral

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fellows, and is followed by presentations from faculty members. Poster sessions featuring research of the students and postdoctoral fellows take place between and after the afternoon sessions. Awards are given for the best oral and poster presentations by a graduate student and postdoctoral fellow. 2006 – 2010: I served on and Chaired the Preliminary Exam Committee of the Molecular and Cellular Pathology Graduate Program in the UNC School of Medicine. The goal of this committee is to design exams to evaluate student comprehension of basic concepts in molecular and cellular pathology and histology. Students in the Ph.D. program are required to take this examination at the end of their second year of graduate study and must pass the exam within two attempts. 2008 – 2010: I was a member of both the Education Committee and the Executive Committee of the Molecular and Cellular Pathology Graduate Program in the UNC School of Medicine. These committees oversee the educational program designed for our graduate students and ensure its success as a program of excellence in the Graduate School at UNC-Chapel Hill. I am the Faculty Mentor for a student-run Marc J. Mass Memorial Lecture Seminar Series held in honor of Dr. Marc J. Mass, a former Ph.D. student in the Department of Pathology and Laboratory Medicine. This event provides an annual opportunity for students to interact with outstanding members of the scientific community. Previous speakers include Drs. Patricia Steeg, Eric Olson, and Peter Libby.

Last amended 3/21/13

Alisa Sue Wolberg, Ph.D. EDUCATION - UNC Health Care · Curriculum Vitae . Alisa Sue Wolberg, Ph.D....

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