Agle hplc2014

1

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web: www.bonnaagela.com

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Guaranteed Product Quality

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Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY

HPLC Columns

SPE Columns

Flash Chromatography Products

Consumables Products

VenusilTM AA KitVenusilTM & PromosilTM HPLC Columns

CleanertTM SPE Cartridges

TLC PlatesCHEETAHTM

ClarinertTM Syringe Filters Vials, Caps and SeptaGC Columns

CleanertTM IC Pre-treatment Cartridges

CleanertTM 96 Wellplates

3

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Bonna-Agela Technologies — A Global Supplier for Chromatography Solutions

2014 Message From Bonna-Agela Technologies

contributed to Bonna-Agela Technologies in some way.

after several years' efforts.

Bonna-Agela teams never stop pursuing new technologies to bridge the gap between conventional HPLC and

new Ultra High Performance LC (UHPLC) systems. Bonna-Agela's sub-2 um Bonshell HPLC columns have

Bonna-Agela has engaged in a new business sector in developing and manufacturing LC hardware and

"Product Focus: chromatography"

Selection from a variety of chromatography media has never been made more easier at Bonna-Agela for your

applications.

Your satisfaction is Bonna-Agela's goal. It is an one-stop call to Bonna-Agela's service and applications

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Terms and Conditions

Acceptance and AvailabilityAll orders placed are subject to the agreement of Bonna-

Agela Technologies Inc. The catalogue does not constitute

an engagement of the company to sell all listed products.

Price and PaymentThe prices are in effect at the time of printing. Bonna-

Agela Technologies reserves the right to change the

customers with advance notice. The prices quoted at the

time of ordering will be guaranteed. The general payment

customers' account information is not satisfactory. A 1.5%

per month service charge will be added to delinquent

ChangesBonna-Agela Technologies reserves the right to change

prior notice and without liability for such changes.

Shipping Policy

United States and Canada. We will try to accommodate

requests for other shipping methods if they are available. All

shipping and handling charges will be billed separately. Should

the carrier.

ApplicationAll products in this catalog should be used for laboratory

or manufacturing use only. They are not intended for direct

medicinal or food use. Bonna-Agela Technologies assumes no

liability for any misuse of the products.

ReturnsBonna-Agela Technologies tries to accommodate all requests

may be restricted by the original manufacturers. Please

contact us for return authorization before returning any items.

WarrantyAll products from Bonna-Agela Technologies are warranted

not warranted for any other particular purpose. Bonna-Agela

Technologies shall not under any circumstance be liable for

TO CONTACT US AND OUR STAFF WILL BE GLAD TO ASSIST YOU.

How to Place Orders

from the following contacts:

Bonna-Agela Technologies US

E-mail: [email protected]

Bonna-Agela India

Bonna-Agela China

Please include the following information with your order or

method of payment and preferred method of delivery. A written

methods of payment.

Checks:

Bonna-Agela Technologies Inc. and send to:

Bonna-Agela Technologies Inc.

Wire Transfer:

information.

Credit Cards:

credit card information.

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001

002

002

002

006

009

011

014

017

017

018

024

028

031

032

033

034

037

045

049

052

052

065

068

068

074

075

077

078

078

079

080

Introduction

HPLC Columns by Family

NEW Products!

UHPLC Technology and Columns

Bonshell columns

Venusil HLP C18

Venusil XBP Polar – Phenyl

Unisol C18 (2) Cloumns

Featured Products!

Unisol Technology and Column Products

Unisol Column

Unisol Amide (HILIC)

Venusil ASB Series Columns (C1, C3, C8, C18 and phenyl)

Venusil PAH

Venusil PFP---USP L43

Venusil XBP COOH

Innoval Columns

Durashell Column

Optimix Family of HPLC Columns

Chiral Columns

Standard Products!

Venusil Family of HPLC Columns

Promosil Family of HPLC Columns

Selected Solutions for Popular Applications

Solutions for Highly Water Soluble Compounds

Solutions for Low pH and High pH Applications

Solutions for LC-MS

Solutions for Fast Analysis

Solutions for Bio-molecules

Solutions for Preparative HPLC

Solutions for SFC

Ordering Information by the Type of Stationary Phases

HPLC Columnsand Media

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HPLC Columnsand Media

Reversed Phase Columns

Normal Phase Columns

Ion-exchange Columns

Mixed Phase Columns

Size Exclusion Columns (Silica Based)

Chiral Columns

Guard Cartridge Holder

HPLC Accessories and Supplies

HPLC Fitting and Tubing

Automatic Column Selector

HPLC Column Heater

UV Lamps

Pipettes and Accessories

HPLC Column Selection Guide

Column Selection Parameters

Basic Considerations in Choosing HPLC Columns

Bonna-Agela HPLC Column Selection Guide

Comparison of Technical Properties for C18 Phases

Cross-reference for Bonna-Agela HPLC Columns

USP Column Selection Guide

Applications

European and American Pharmacopeia

HILIC Applications

Antibiotics

Synthetic Antimicrobial Agents

Anti-virus Medicine

Steriod Hormones

Medicine for Gastric Ulcer

Analysis of Alkaloids

Agricultural Chemical

Analysis of Amino Acids

Applications in LC-MS

Others

APPENDIX

Procedures for Column Regeneration

Methods of Maintaining Good Column Lifetime and

Performance

080

085

088

088

089

089

090

091

091

091

091

091

092

093

093

093

094

094

095

098

101

101

109

115

118

119

121

123

124

126

127

128

130

134

134

135

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1. Unisol C18

and neutral organic compounds. It has a superior performance compared to other branded C18 phases in almost every aspect.

2. Unisol C18(2)

concentration.

3. Unisol Amide - It has a unique HILIC mechanism to retain and separate molecules of strong polarities. Also it can be used as either

to separate very polar compounds for optimized retention.

Optimix Series –A class of column provides optimized selectivity and balanced retention for hydrophilic and hydrophobic organic

5. Durashell Series

Venusil ASB Series

Bonshell Columns – Bonshell columns represent a new level of performance through a core-shell structure. They are the best

8. Innoval Columns-

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UHPLC Technology and Columns

The chromatography separation technology has been revolutionized by Ultra-Performance Liquid Chromatography (UHPLC) System. Sub-2 um

particles surface.

silica has narrow size distribution and no dead pores whose pore size is smaller than 2nm.

phase.

UHP AQ C18

2

Main Features:

Low pH stability: stable at pH as low as 1.5;

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Pressure test

Balanced Retention for Hydrophilic and Hydrophobic Compounds

UHP ASB C18

2

Main Features:

Polar C18 phase: Very strong separation power for polar compounds

Sample: Uracil (1) Phenol (2) Nitrobenzene (3) and

Flow R

(1)(1)

(2) (2)

(3) (3)

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Pressure test

UHP Innoval C18

(1)

(2)

(3)

Characteristics:2

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Test Conditions

columns.2

Main Features:

Pressure test

Type

UHPLC

UHPLC

UHPLC

UHPLC

UHPLC

Dimension (mm) UHP AQ C18 UHP ASB C18 UHP Innoval C18 UHP HILICParticle size (µm)

Ordering Information

UHP HILIC

2

NEW Products!

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Main features:

Main features:

Bonshell ASB C18 columns

Bonshell columns

------A better choice to speed up your HPLC analysis

Core-shell technology

Core-shell technology

Very strong separation power for polar compounds

conditions.

The structure of core-shell silica The structure of traditional silica

Stationary phase characteristics

Phase

ASB C18

C18

Particle size Pore size Carbon loading Surface Area2

2

End-capped style

No

Double

pH range

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Lower pressure

particles

2

Rurpe curve': Bonshell

Pressure (bar)

Pressure

Bonshell ASB C18

Sub-2 m C18 column

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Order information

Bonshell C18 columns

Time

5

13

Water% ACN%

25

caproaldehyde

Mobile phase:

Bonshell ASB C18 Bonshell C18Type

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Bonshell columns

Dimension (mm)Particle size (µm)

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Venusil HLP C18

Venusil HLP C18 column is the improved product for Venusil HLP. It is reversed-phase column based on Bonna-Agela's amide

amide polar group which literally capped the silica's residual silanols from interacting with highly basic compounds. It can be used to

2

Main Features:

High Loading Capacity

Sample: Amitriptyline

Temperature: Ambient

Temperature: 25

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Ordering Information2

Type

Analytical

Analytical

Analytical

G

DCG

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG


3

3

3

3

3

5

5

5

5

5

5

5

HLP C18

G: Guard Cartridge

DCG: Direct-connection Guard Cartridge Holder

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Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG


5

5

5

5

5

5

5

5

5


2

Main Features:

Different selective with reversed-phase C18 or C8 column;

Greater separation between polar and aromatic compounds with only slight differences chemically or structurally.


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Phase Particle size Pore size Carbon loading Surface Area End-capped style pH range

AQ C18 15 %2

Single

Characteristics

Innoval AQ C18 column

Main Features:Strong separation performance for polar and isomer compounds

Lower surface area leading to good contamination tolerance

Long life-time of Innoval AQ C18

1st injection

Mobile phase B: Methanol

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Order Information2

Dimension (mm)

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Type

Analytical

Analytical

Analytical

Analytical

G

Semi-preparative

Semi-preparative

G

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

G

Analytical

Semi-preparative

Semi-preparative

G

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

G

Innoval AQC18

G: Guard Cartridge


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Unisol C18 (2) column is the improved product for Unisol C18. It is a universal reversed phase column through carefully optimizing the

good retention capability for the different polar compounds. Because it is less affected by the pH of mobile phase and buffer

Unisol C18 (2) Cloumns

Characteristics:2

Main Features

The activity testing of silicon hydroxyl

of basic compound is less changed when the pH of mobile phase changes

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Sample: uridine

Mobile Phase: water

Good tolerance of biological matrix

Sample: uridine in plasma

then wash column with solution

.

100 % Water compatible

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Applications

Adenosine Cyclophosphate

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Main Features:

hydrophobicity in the reverse phase mode

( "hot spots" )

Bonna-Agela's unique sol-gel process technology generates a uniform surface that produces a universal column applicable to many

Synthetic silica with high purity is typically obtained by hydrolyzing silicic acid esters followed by high temperature sinteration. The

covered surface. It will create some isolated silanol groups with very strong acidity or some "un-leached islands" during the leaching

further contributing to the nonuniformity of the surface.

silane is adsorbed evenly on the silica surface. It is subsequently hydrolyzed by a controlled amount of moisture. A fresh layer of silanols

retention).

Unisol Technology and Column Products

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compounds. We offer bonding chemistry of C8 groups presenting different selection for various application.

Main Features:

Unisol Column

numbers in Italic are for amitriptyline.

Waters-Atlantis

12071

5

4010

83

Unisol C18

8

Inertsil C18

Brand A C18

Waters erra C18

1638

9518

153

4533

6772


C18

C8

18 % 2

2

Single

No

Characteristics

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Good Retention for Polar Compounds

2PO

Temperature: 35


2PO

Methanol

Temperature: 23

Sample: Uracil

Propranolol hydrochloride

Butyl Phthalate

Dimethyl Phthalate

Naphthalene

Acenaphthene

Amitriptyline

min

mAU

8.2

33

min

mAU

1 3

.583

58.3

22

min

mAU

1

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Extremely Low Metal Effects

Temperature: 25

,

min1 2 3 5

mAU

TF=1.31

min1 2 3 5

mAU

2.2

2.8 TF

min1 2 3 5

mAU

5

15

25

35

2

3

min2 8

mAU

5

15

25 3

5

TF TF=2.88

Good Tolerance of Directly Injected Biological Matrix

Run Time: 8 min

Fresh Colunm

After column washing and re-equlibrium in

Plates =

Plates =

1 2 3 5

1 2 3 5

1 2 3 5

mAU

-1

mAU

-1

mAU

-1

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Enable Methods to be Compatible with LC-MS

2PO Aq.

without a Non-Volatile Ion Pair Reagent Using Unisol C18

Column .

51 52 53

mAU

min

Compatibility with 100 % Aqueous Mobile Phase

Sample: Uridine

during the testing period for each column

mAU

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

mAU

Unisol C18

s

mAU

Luna C18 initial

mAU

l

mAU

s

mAU

s

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Greater Volume Loading Capacity Than Other Brands

min1 2 3 5

mAU

15

min1 2 3 5

mAU

15

15

min1 2 3 5

mAU

Brand A

Plates=2118

mAU s

mAUs

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Perfect Peak Symmetry and Good Stability at Mid-pH for Basic Compounds

pH=7.0

mAU

35

25

15

5

Initial

mAU

35

25

15

5

2.31

1

A

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Unisol Amide stationary phase is made from ultra pure spherical silica particles bonded with a neutral hydrophilic amide group. It can be

2 and

2

because of its difference in separation mechanism from most of the conventional stationary phases.

.2

Main features:

Applications:

Separation of Validamycin

OO

NH

CH2OH

HOH2C

OH

OH

CH2OH

OHHO

OH

OH

HO OH

Au

2

min

Temperature: 25

Sample: Validamycin

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Separation of Shikimic Acid

Mobile Phase: A:1 % Fomic Acid in Water; B: Acetonitrile

Temperature: 25

Water-soluble Vitamins

2

mi n1 2 3 5

mA U D AD 1 S ig =2 R ef =3 ( AG EL D)

1 2.3

3.3

5.5

mi n1 2 3 5

mA U

15

25

D AD 1 Sig = Ref =3 ( AGEL D)

158

1

3.5

2PO

Temperature: 25

Sample: Validamycin

Au

min

2

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mi n1 2 3 5

mA U

15

D AD 1 S ig =2 R ef =3 ( AGEL 11 .D )

3.2

3 3.8

35


Type Dimension (mm)Particle Size (µm) Unisol C8Unisol C18 Unisol C18 (2) Unisol Amide (HILIC)

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

2.5

2.5

2.5

2.5

2.5

2.5

2.5

2.5

3

3

3

3

3

3

3

3

3

3

3

3

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2

G: Guard Cartridge

DCG: Direct-connection Guard Cartridge

Type Dimension (mm)Particle Size(µm) Unisol C18 Unisol C8Unisol C18(2) Unisol Amide(HILIC)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Preparative

Preparative

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Featured Products!

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Venusil ASB Series Columns (C1, C3, C8, C18 and phenyl)

Main features:

Polar C18 phase: Very strong separation power for polar compounds

Five different bonded phases provide broad selectivity

mAU

mAU

min

min

min8

5

15

25

35

mAU

Sample: Naphthol

The Stability in the Low pH

Venusil ASB column

The Separation of Organic Acid

Phase Particle size Carbon loading Surface Area pH rangeEnd-capped stylePore size

C18

C8

C3

C1

phenyl

C18(T)

C8(T)

12 %

2 %

5 %

3 %

2

2

2

2

2

2

2

No

No

No

No

No

No

No

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Highest LC-MS Sensitivity

2

2-3 times higher than many popular brands

Type Particle VenusilASB C18

VenusilASB C8

Venusil VenusilASB C1

VenusilASB Phenyl

Analytical

Analytical

Analytical

Analytical

G

DCG

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Dimension(mm)

283

223

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2


VenusilASB C8

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytica

G

DCG

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


Dimension(mm)


VenusilASB C8


VenusilASB Phenyl

Dimension(mm)

Preparative

Preparative

Preparative

Preparative

Preparative

G: Guard Cartridge


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Venusil PAHVenusil PAH column is a polymerically bonded C18 column. It is recommended for the separation of PAHs and steric isomers of the

soil and food is of major importance.

2

Main Features:High resolution separation of PAHs

Robust and reproducible performance

Good for applications requiring separation of geometric isomers

Mobile Phase:


Type

Analytical

G


5

5

Venusil PAH

Time (min)

2

H2O (%)

15

15

5

5

MeOH (%)

85

85

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Venusil PFP---USP L43

2

Main Features:

Mobile Phase: MeOH: CH3 3COONH

Dopamine Isoprenaline

.D: UV Chromatogra nm

Intens.

mAU

5 15 25 35 Ti me mi

Dopamine and Its Metabolin

OH

HO

NH2

HO

HO

OH

NH


G: Guard Cartridge



3

3

3

3

3

5

5

5

5

5

5

5

Type

Analytical

Analytical

Analytical

G

DCG

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Venusil PFP

VF

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Venusil XBP COOH

Characteristics:2


2


5

5

5

5

5

5

5

5

5

5

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Venusil XBP COOH

G: Guard Cartridge


2PO

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Metal sensitivity testing

Temperature: 35


C18

Silica

2

2

Double

No

Characteristics

Batch-to-Batch Reproducibility

Main Features:

Lower surface area leading to good contamination tolerance

Temperature: 25

Innoval Columns

Silica.

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toluene

acetic acid pH

Temperature: 25

and p-nitroaniline


The test show that Innoval has a lower metal content than Brand H

RT(min)Column

Brand H

Innoval

Brand H

Innoval

Compounds TF

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The content of ferulic acid (acid samples)

Temperature: 25

Order Information

2

Dimension (mm)

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Type

Analytical

Analytical

Analytical

Analytical

G

Semi-preparative

Semi-preparative

G

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

G

Analytical

Semi-preparative

Semi-preparative

G

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

G

Innoval Silica Innoval C18

G: Guard Cartridge


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Featured Products!

We also develop a new phase Durashell C18(L). It gives a good performance by decreasing the surface area to achieve the balance of

Phase

C18

C18(L)

C8

phenyl

NH2

Particle size Pore size Carbon loading

21 %

13 %

Surface Area2

2

2

2

2

End-capped style

Double

Double

Single

Single

No

pH range

Durashell Column

Characteristics

Main Features

Durashell C18 Vs Brand G C18

Mobile phase: MeOH: H2O=85:15

Flow rate:

Sample:

Injection:

Temperature:

Column: Bonna-Agela

Agela

Durashell C18

Durashell C18 (L)

Durashell RP

Waters

Gemini-NT C18

The cross–reference for Agela HPLC columns

HP

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Bonna-Agela Durashell C18 Brand G C18

Test of basic compounds under basic condition:

Mobile phase: MeOH: :

Flow rate:

Injection:

Temperature:

Sample:

Bonna-Agela Durashell C18 Brand G

Peak No.

3

Retention time N Tf AsymmetryCompound

Amitriptyline

Peak No.

3


Amitriptyline

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Featured Products!

Test of basic compounds under acid condition:

Bonna-Agela Durashell C18 Brand G

Peak No.

3

Retention time N Tf Asymmetry

1.122 1.238

Compound

Amitriptyline

Peak No.

3


Amitriptyline

The maximum volume of sample loading onto Durashell C18 and Brand G C18

Sample: Amitriptyline dissolved in mobile phase

Peak No.

1


Amitriptyline

Peak No.

1


Amitriptyline

Durashell

Amitriptyline

Brand G

Amitriptyline

HP

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Column

Durashell C18

Brand G

Column

Durashell C18

Brand G

N Tf

1.11

Tf

Retention time/min

Retention time(Amitriptyline)/min

Test of basic compounds under basic condition:

Test of basic compounds under acid condition:

Column

Durashell C18

Brand G

Tf

1.122

Amitriptyline (min.)

The maximum volume of sample loading onto Durashell and Brand G:

Sample amount (mg)

Durashell C18

Brand G

Rt (min.)

Tf

Rt (min.)

Tf

High pH Stability of Durashell C18

Temperature: 35

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Best Value


Featured Products!

Durashell C18(L)

Sample: BSA peptide fragment

Time

5

A

82%

5%

5%

B

5%

8%

18%

32%

5%

First injection The thousandth injection

Rat liver protein extracts of enzyme peptides

sodium

Heptanesulfonate (acetic acid pH=3.5)

;

Time

2

13

22

23

A

82%

5%

5%

B

5%

8%

18%

32%

5%

HP

LC

Colu

mns


Featured Products!

Durashell C8

Applications

Glucosamine Hydrochloride Tablets

Durashell NH2

o m-nitroaniline and

p

Column: Durashell NH2

Detector: Differential detector

HP

LC

Colu

mns


Best Value


Featured Products!

Mobile Phase: Water: ACN =15:85

Glucose and Sorbitol

4-Hydroxy-D-phenylglycine

Sialic acid

Durashell RP

HP

LC

Colu

mns



2

Durashell RP: 2

Type Dimension(mm)

Durashell C18

Durashell C18 (L)

DurashellPhenyl

Durashell C8Durashell NH2Particle

size (µm)

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Preparative

Preparative

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Durashell RP

G: Guard Cartridge


Featured Products!

HP

LC

Colu

mns


Best Value


Optimix Family of HPLC Columns

selectivity compared to a mono-chain type stationary phase.

HPLC conditions:

Column

Mobile Phase

ACN

Flow

Wavelength

Injection

Sample

analyzed in a single run under isocratic conditions.

Optimix C18/Amide

Features:

Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions

Featured Products!

HP

LC

Colu

mns


Featured Products!

Optimix C18/C8:

Main Features:

compounds.

Analysis of Vancomycin and Its Impurity

NH2

H3C

HO

CH3

OHHO

OH

OH

CH3

CH3

CI

CI

O

O

OO

OO

O

O

O O

O

O

O

HO

HO

HOOC

H

H H

H HH

3C

NH2

HHHHN

NH

HN

NH

NH

HN

HN

H

OHOH

NH2

H3C

HO

CH3

OHHO

OH

OH

CH3

CH3

CI

O

O

OO

OO

O

O

O O

O

O

O

HO

HO

HOOC

H

H H

H HH

3C

NH2

HHHHN

NH

HN

NH

NH

HN

HN

H

OHOH

Vancomycin Impurity

Vancomycin

Impurity

2

1

1

8.5e+1

1

1

1

1

1

5.5e+1

1

1

1

3.5e+1

1

2.5e+1

1

1.5e+1

1

5.Time

12.52

11.52 Temperature: 25

Sample: Vancomycin+Impurity

HP

LC

Colu

mns


Best Value


Optimix C18/SCX

Main Features:

Application in Melamine Detection Standard Curve

HPLC Conditions :

Mass Conditions:

Impurity

Vancomycin

2

1

1

8.5e+1

1

1

1

1

1

5.5e+1

1

1

1

3.5e+1

1

2.5e+1

1

1.5e+1

1

5.

Time

Featured Products!

HP

LC

Colu

mns


Milk Sample

Melamine Standard

G: Guard Cartridge DCG: Direct-connection Guard Cartridge

2

Type Dimension (mm) Particle size (µm) Optimix C18/C8 (1:1) Optimix C18/amide (1:1) Optimix C18/phenyl (1:1)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

3

Featured Products!

HP

LC

Colu

mns


Best Value


Featured Products!

Chiral Columns

Normal phase chiral columns from Bonna-Agela Technologies are based on modified celluloses and starches. This type of chiral columns

Main Festures:

ide selectivity and high loading capacity

Venusil CA

acid compounds

Example:

Temperature: 25

L-val PMBD-val PMB

N

O

N

N N

NHOPMB

O N

O

N

N N

NHOPMB

O

HP

LC

Colu

mns


isomer 1 isomer 2

Venusil CO

Example:

Temperature: 25

Venusil CJ

acid compounds.

Example:

OHN

O

HN

O

O

OHN

O

HN

O

O

min5 15 25 35

Norm.

-5

15

25

VWD1 A Wavelength=2 nm .D)

1

2

Featured Products!

HP

LC

Colu

mns


Best Value


Featured Products!

Chiral Amide-1

Main Features:Can be used as reversed phase or normal phase

Unique selectivity and high resolution

More stable and longer column lifetime

SiCH2CH2CH2HNCOON

N

OH3C

2PO



5

5

5

5

Type

Analytical

Analytical

Preparative

Preparative

Analytical

Preparative

Preparative

Venusil CA Venusil CO Venusil CJ Chiral Amide-1

HP

LC

Colu

mns


Introduction of Venusil HPLC Columns

Unprecedented Experience in Chromatography

Venusil Family of HPLC Columns ------ A Full Line Products of High Quality

Venusil HPLC columns are manufactured from the ultra pure spherical silica particles. The outstanding properties of the HPLC phases

are results of subjecting high quality silica to Bonna-Agela's innovative surface modification and unique bonding processes. Our

columns was developed to meet the tough requirements for the analysis of highly polar compounds. Lot-to-lot reproducibility is ensured 2 2 2

outperforming competitors.

The pH-independent high performance feature of

Venusil columns allows scientists to establish rugged

Waters Symmetry-C18 Zorbax XDB-C18 Venusil XBP C18 (2)

Spec. on tailing factor(toluene)

Spec. on tailing factor-amitriptyline

pH range

Carbon %

and mobile phase

Highest surface coverage

A wide variety of chemistries

Minimum buffer concentration needed

Great column lifetime

Tolerance of large volume and mass injection

Better and faster separation

Lower cost; greater convenience

1.8

12 % 18 %

Characteristics of Venusil Columns

Standard Products!

HP

LC

Colu

mns


Best Value


Standard Products!Expanding the Capabilities with the Venusil Family of C18 Columns

Bonna-Agela Technologies has developed a series of high quality C18 stationary phases to meet a wide range of application needs.

are able to tune the surface properties of the silica particles and alter selectivity to meet a variety of application requirements.

other columns)

compounds:

Many of our columns have very unique properties to address some special needs. Here are some examples:

HP

LC

Colu

mns


Venusil XBP C18 (2) Series Columns

Main Features:

suitability

Hydrophobicity Test

Inertness Test

Characteristics:

2

2PO 2HPO


Mobile Phase: NaH2PO 2HPO

Amitriptyline is a strong basic antidepressant. It is recognized

min

1 2

3 5

mAU

12

Standard Products!

HP

LC

Colu

mns


Best Value


Standard Products!Extremely Low Metal Effects Selectivity of Basic Compounds

Temperature: 35


Type Dimension(mm) Venusil XBP C18 (2)Particle size(µm)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


Venu C18(2)

Part.No.:V 2

Brand Y C18

Brand H

HP

LC

Colu

mns


Plates (Toluene) Tailing Factor

Comparison of C8 Phases

Brand L C8

1.12

1.15

Phase Particle size Pore size Carbon loading Surface Area End-capped sty pH range

22 %

15 %

8 %

8 %

3 %

12 %

5 %

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

Double

Double

Double

Single

Double

Single

Single

No

No

No

No

Single

No

No

No

1.5-8.5

1.5-8.5

1.5-8.5

1.5-8.5

1.5-8.5

1.5-8.5

C18

C18(L)

C18(T)

C8

C18 (A)

C8(L)

C8(T)

C1

NH2

phenyl

CN

Silica

Silica(L)

Silica(T)

Characteristics:

Venusil XBP Series Columns

density and therefore provide highest hydrophobicity or

lowest polarity. This allows for the least interaction

Trimipramine

Main Features:Inertness: one of most base-friendly columns.

large injection volumes.

M triethylamine.

2

2.0

16

2.9

04

0.7

41

1.1

87

V C8

1.1

90

2.4

07

Luna C8

Initial

0.6

07

0.7

01

0.6

18

6.0

47

17.0

21

0.7

27

9.7

76

Quality of Column Packing Perfect Peak Symmetry and Great Lifetime of the Column for Basic Compounds at Mid-pH

Standard Products!

Mobile Phase:

HP

LC

Colu

mns


Best Value


Standard Products!Ordering Information

Type Particle Dimension (mm)

Venusil XBP C18 Venusil XBP C18 (A)

Venusil XBP C8 Venusil XBP C4 Venusil XBP C1

2

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Preparative

Preparative

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


HP

LC

Colu

mns


2

2

Type

Type

Particle

Particle

VenusilXBP C18

VenusilXBP NH2

VenusilXBP C8

VenusilXBP Phenyl

VenusilXBP C4

VenusilXBP CN

VenusilXBP C1

Venusil XBP Silica

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


Dimension(mm)

Dimension(mm)

2

Type Particle VenusilXBP NH2

VenusilXBP Phenyl

VenusilXBP CN

VenusilXBP Silica

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

5

Dimension(mm)

Standard Products!

HP

LC

Colu

mns


Best Value


Standard Products!Venusil XBP(L) Series Columns


(L) (L) has relatively low surface area which allows

(L)

PhaseParticle

sizeCarbonloading

SurfaceArea

End-cappedstyle pH range

C18(L)

C8(L)

Silica(L)

15 %

8 %

2

2

2

Double

Single

No

1.5-8.5

Pore size

2

Type Particle VenusilXBP C18(L)

VenusilXBP C8(L)

VenusilXBP Silica(L)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

Dimension(mm)

G: Guard Cartridge


HP

LC

Colu

mns


Venusil AQ C18 Columns

Main Features:

than other brand AQ columns.

Low pH stability (pH=1.5): better stability than most of the

popular brand columns (including other brand AQ columns)

Venusil AQ

Characteristics:2

Compatibility with 100 % Aqueous Mobile Phase

Sample: Uridine

during the testing period for each column

mAU

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

2 12 18 min

mAU

mAU

Luna C18 initial

mAU

l

mAU

s

mAU

s

Standard Products!

HP

LC

Colu

mns


Best Value


Venusil

Part No.: V

Brand Y


Great Peak Shape for All Type of Compounds

2PO 2HPO

Flow Rate

amitriptyline

Temperature: 23

Flow Rate

min2 8 12 18

mAU

Venusil

Part No.: VA

Brand Y

mAU

min12 18

Standard Products!

HP

LC

Colu

mns



2

Type Dimension(mm)

Particle VenusilAQ C18

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Preparative

Preparative

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


Standard Products!

HP

LC

Colu

mns


Best Value


Venusil SCX Columns


2

Type

Phase

Phase

Type

Particle size

Particle size

Venusil SCX

Carbon loading

Carbon loading

Surface Area

Surface Area

End-capped style

End-capped style

pH range

pH range

Venusil SCX

Analytical

Analytical

Analytical

G

DCG

3.5 %

2

2

2

2

No

No

No

No

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

Dimension (mm)

Pore size

Pore size

Dimension (mm)

G: Guard Cartridge


Venusil SAX Columns


2

2

Type

Type

Venusil SAX

Venusil SAX

Analytical

Analytical

Analytical

G

DCG

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

5

Dimension (mm)

Dimension (mm)


Standard Products!

HP

LC

Colu

mns


Type Venusil Diol

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

5

5

5

5

5

5

5

5

5

Dimension (mm)

Venusil Diol

Standard Products!

HP

LC

Colu

mns


Best Value


Promosil Family of HPLC Columns

through Bonna-Agela Technologies' well controlled bonding process. They have high surface bonding coverage and are completely

value.

Main Features:

2PO 2HPO

Hydrophobicity Test

Promosil C18

Characteristics:

2

min1 2 3 5

mAU

12

Phase Particlesize

Carbonloading

SurfaceArea

End-cappedstyle

C18

C8

NH2

CN

Silica

18

5

2

2

2

2

2

Double

Double

No

Single

No

5

5

5

5

5

Pore size pH range

Standard Products!

HP

LC

Colu

mns



Mobile Phase: NaH2PO 2HPO

Hydrophobicity Test

mAU

-5

15

5

2

1 2

.335

Promosil C8

Promosil CN

Temperature: Room temperature

Promosil Silica

Standard Products!

HP

LC

Colu

mns


Best Value



Dimension(mm)

Particle PromosilC18

PromosilC8

PromosilNH2

PromosilCN

PromosilSilica

5

5

5

5

5

5

5

5

5

5


Standard Products!

HP

LC

Colu

mns


Solutions for Highly Water Soluble Compounds

stationary phases along with development schemes to help chemists systematically develop methods for the analysis of highly water-

hydrophilic interaction with the analytes. Unisol Amide provides the strongest retention of hydrophilic compounds among these three

types of columns.

HPLC separation of four organic acids using Venusil AQ C18

was not adequate for analysis on either an AQ C18 or an ASB C18 column.

the desired resolution. When the desired retention or resolution cannot be obtained for highly water soluble compounds using a AQ C18

reversed phase columns and offer much higher retentivity .

The multiple selection of columns and this comprehensive method development scheme for the separation of highly water-soluble

compounds will allow you to develop HPLC methods at ease.

Venusil AQ C18

Venusil ASB C18

Unisol Amide

HP

LC

Colu

mns


Best Value


35

25

15

5

Area:

21.2

53

mA

U

min

112.2

23

mA

U

min

HPLC Separation of Organic Acids

HPLC Analysis of Validamycin Raw Products

HPLC Analysis of Shikimic Acid

OO

NH

CH2OH

HOH2C

OH

OH

CH2OH

OHHO

OH

OH

HO OH

Validamycin

B:Acetonitrile

Temperature: 25

Sample: Validamycin

HP

LC

Colu

mns


Other Stationary Phases from Bonna-Agela Technologies for Hydrophilic Compounds Separation:

Highly Water Soluble Compounds

Enough ResolutionNot Enough Retention

AcidicCompounds

BasicCompounds

NeutralCompounds

use Unisol Amide

and adjust

adjust pH to

use Unisol

Amide

use Venusil ASB C18

Amide

- Unisol C18 2

- adjust mobile phase

ratio or pH

- use Venusil ASB C18 or

Unisol Amide

HP

LC

Colu

mns


Best Value


Teicoplanin

OH

OH

OH

OH

OH OH

HOOC

HOHO

HOHOHO

HO

HN

NH

NH

NH

NH

NH2

HN

HN

HO HO

H3C

CI

NHR

CI

H

H H

H

OO

OO

O

O

O

O

O

O

O

O

O

O

R

O

A2-1

A2-2

A2-3

A2

A2-5

d8-methylnonanoic aicd n-decanoic aicd8-methyldecanioc aicd

Teicoplanin

NO2

CH O

CH3

O

O

NH

COOH

OH

Nicousamide

1.

2.

3.

5.

8.

Time

1

Unisol C18

Mobile Phase: A:NH

B:ACN

Sample: Teicoplanin+Impurity

Temperature: 35

3PO )

Sample: Nicousamide

HP

LC

Colu

mns


HILIC Column Family From Bonna-Agela Technologies

Unisol Amide (Bonna-Agela HILIC )

Venusil XBP Silica (Bonna-Agela HILIC II)

Venusil XBP NH2 (Bonna-Agela HILIC III)

2

Comparison:

2

2 have better retention of acidic compounds.

Unisol Amide has the best reproducibility and lifetime because of a bonded and close-to-neutral protection layer.

Water-soluble Vitamins

VB1

VB1

VC VB2

VC

VB2

VB1VCVB2

HP

LC

Colu

mns


Best Value


Initial

After aging

VB1

VB2

VB6

VC

VB1

VB2

VB6

VC

Initial

After aging

VB1VB2

VB6

VC

VB1 VB2

VB6

VC

Stability Comparison of Unisol Amide and Venusil XBP Silica

Aging Conditions:

2PO

HPLC Conditions:

Temperature:

Time (min)

Time (min)

Time (min)

Voltage (

mV

)V

oltage (

mV

)

Voltage (

mV

)V

oltage (

mV

)

Time (min)

HP

LC

Colu

mns


Low pH Stability of Venusil ASB C18

High pH Stability of Durashell C18

Solutions for Low pH and High pH Applications

Sample: Naphthol

Temperature: 35

applications and method development needs.

Initial

A h

HP

LC

Colu

mns


Best Value


Solutions for LC-MS

Pseudoephedrine in Plasma

Bonna-Agela Technologies provides columns that can meet the needs of LC-MS applications. The most popular LC-MS columns from

Bonna-Agela include:

Mobile Phase: A:1 % Formic Acid in Water;

B:Methanol;

Temperature: 25

Pseudoephedrine

Pseudoephedrine

.8 1. .2 1. 1.82 2.22 2. .8 3. .2Time.min

2.12

1.2e5

1.8e5

2.2e5

2.8e5

Lignocaine

.8 1. .2 1. 1.82 2.22 2. .8 3. .2Time.min

1.5e5

2.5e5

3.5e5

HP

LC

Colu

mns


Analysis of 14 Kinds of Hormone

Oleic Acid in Plasma

B:Acetonitrile

Temperature: 25

Mobile Phase: A:Water; B:MeOH

Temperature: 25

Oleic acid

Highest LC-MS Sensitivity

2-3 times higher than many popular brands

283

223

HP

LC

Colu

mns


Best Value


Sample: Uracil (1) Phenol (2) Nitrobenzene (3) and

Solutions for Fast Analysis

Small particle size or fused-core technology

UHP AQ C18

UHP ASB C18

UHP Innoval C18

UHP HILIC

Bonshell ASB C18

Bonshell C18

Mobile Phase: A: NH

B: ACN


Temperature: 35

Mobile Phase: A:NH

B:ACN


Temperature: 35

(1)

(2)

(3)

HP

LC

Colu

mns


Sample Polysaccharide

Durashell:

loadability for basic compounds.

Solutions for Bio-molecules

ensure the surface inertness.

Solutions for Preparative HPLC

Bonna-Agela Technologies offer a full line of preparative HPLC columns to meet a variety of application needs.

Our preparative columns feature:1. Great scalability

3. High loading capacity

Unisol-Amide:

solution for purifying highly polar compounds.

Preparation of Polysaccharide

2; HILIC

See the section of "ordering information by type of stationary phase" for ordering or call our technical staff for helps in

selecting the columns you need.

General guide for the column selection:

HP

LC

Colu

mns


Best Value


Solutions for SFC

Unisol Amide

2

Venusil HLP C18:

with highly basic analytes.

See the section of "Ordering Information by Type of Stationary Phase" for ordering or call our technical staff for helps.

High Loading Capacity



HP

LC

Colu

mns


Reversed Phase Columns

Reversed Phase Columns for Small Molecules

Analytical Columns

2

C%=18

2 2

C%=5

2

C%=12

2 2

C%= 3

2

C%=5

Dimension(mm)

PromosilC18

PromosilC8

PromosilCN

Venusil XBP Phenyl

Venusil XBP C4

Venusil XBP C1

Venusil XBP CN

Dimension (mm) UHP AQ C18 UHP ASB C18 UHP Innoval C18 UHP HILICParticle size (µm)

UHP AQ C182

C %=8 %

UHP ASB C182

C %=8 %

UHP Innoval C182

UHP HILIC2

C %=3 %

Dimension (mm) Bonshell ASB C18

2

Bonshell C18

2

Particle size (µm)

HP

LC

Colu

mns


Best Value


2

C %=18

2

C %=15

2

C %=15

2

C %=15%

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

IA

2

C%=15

2 22

C %=15

Dimension(mm)

Venusil XBP C18

(2)

Venusil XBP C18

(L)

Venusil XBP Polar –

Phenyl

Venusil HLPC18

Venusil PFP

Venusil PAH

InnovalC18

InnovalAQ

G: Guard Cartridge


Particlesize (µm)

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

HP

LC

Colu

mns


3

3

3

3

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

2

C %=12 m2

m2

2

C %=18

2

C %=18

2 2 2

m2

Dimension(mm)

Particle VenusilASB C18

DurashellC18

DurshellC18 ( L)

DurashellRP

VenusilAQ C18

VenusilXBP C18

UnisolC18

UnisolC18(2)

VenusilXBP C8


HP

LC

Colu

mns


Best Value


3

3

3

3

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

2 2 2

C %=2

2

Dimension(mm)

Particle Size VenusilASB C8


DurashellC8

G: Guard Cartridge


Reversed Phase Columns for Large Molecules

2

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Column Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CN

HP

LC

Colu

mns


2

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Preparative

Preparative

Preparative

Preparative

Preparative

G

G

Column Type Dimension (mm) Venusil ASB C18

low bleed

C8 for low pHand low bleed

highesthydrophobicity

General RP

Venusil ASB C8 Venusil XBP C18 Venusil XBP C8

G: Guard Cartridge


Preparative

Preparative

Preparative

Preparative

Preparative

G

G

Column Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CN

HP

LC

Colu

mns


Best Value


Normal Phase Columns

Normal Phase Columns for Small Molecules

TypeParticle Unisol Amide

(HILIC)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

Preparative

Preparative

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

2

Venusil XBP Silica

(HILIC II)

2

Venusil XBP NH2

(HILIC III)

2

Venusil Diol

2

Venusil XBP CN

2

G: Guard Cartridge


Dimension(mm)

HP

LC

Colu

mns


Type Particle Size (µm) Dimension (mm) Promosil Silica Promosil CN Promosil NH2 Durashell NH2

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


22 2 2 2 2 2

HP

LC

Colu

mns


Best Value


Normal Phase Columns for Large Molecules

2

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

G

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

G

G

Preparative

Preparative

Preparative

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

TypeParticle Venusil

XBP NH2

Venusil Diol


Venusil XBP CN

Venusil XBP Silica

Dimension(mm)

HP

LC

Colu

mns


5

5

5

5

5

Analytical

Analytical

Analytical

G

DCG

TypeParticle Size Venusil

SAXVenusil

SCX

2

Dimension(mm)

G: Guard Cartridge


Mixed Phase Columns

2

Type Dimension (mm) Particle size (µm) Optimix C18/C8 (1:1) Optimix C18/amide (1:1) Optimix C18/phenyl (1:1)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

3

Analytical

Analytical

Analytical

G

DCG

2 2

5

5

5

5

5

Type Particle Size VenusilSAX

VenusilSCX

Ion-exchange Columns

Dimension(mm)

G: Guard Cartridge


HP

LC

Colu

mns


Best Value


Chiral Columns

Type Dimension (mm) Particle size (µm)Optimix C18/SCX (1:1) Optimix C18/SAX (1:1)Optimix C18/Nitrophenyl (1:1)

Fast analysis

Fast analysis

Fast analysis

Fast analysis

G

DCG

Fast analysis

Fast analysis

Fast analysis

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

G

DCG

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

5

5

G: Guard Cartridge


Analytical

Analytical

G

DCG

Preparative

G

G

Type Venusil COVenusil CA Venusil CJDimension (mm)

Size Exclusion Columns (Silica Based)

Dimension(mm)Type

Particle SizeGF GF amide

5

5

5

5

Analytical

Analytical

Analytical

Analytical

Semi-prep.

Semi-prep.

Semi-prep.

Semi-prep.

HP

LC

Colu

mns


Analytical

Analytical

G

DCG

Preparative

G

G

Type Chiral Amide-1 Dimension (mm)

Guard Cartridge Holder

Analytical

Analytical

Type P/N

2.1

Dimension (mm)

Direct-connection Guard Cartridge Holder

Preparative

Type P/NDimension (mm)

Preparative


Analytical


HP

LC

Colu

mns


Best Value


HPLC Fitting and Tubing

Automatic Column Selector

HPLC Column Heater

Compact design and easy access to columns

Compatible with any HPLC

Cat.No.

Operating Temperature

Temperature Accuracy

Compatible Column Quantity and Length

Power

Weight

Above 5

MaterialCat.No. Tubing Size/Nut Threads

Bonna-Agela Technologies offers UV lamps of the same or better quality as a low cost

Shimadzu.

UV Lamps

HP

LC

Colu

mns


Shimadzu

UV AA

Original Instrument Part Number Bonna-Agela Lamps Part Number

Pipettes and Accessories

Mechanical Pipette (Fixed Volume)

Mechanical Pipette (Adjustable Volume)

Single Channel Pipette

8-Channel Pipette 12-Channel Pipette

5

25

Cat No. Cat No.

1

5

1

1

5

5

5

Cat No.

Cat No.

Cat No.

Cat No.

Pipette Tips

Cat No. Cat No.Qty Qty

HP

LC

Colu

mns


Best Value


Column Selection Parameters

Basic Considerations in Choosing HPLC Columns

Stationary Phases

preparative separations; use shorter chain if the retention

for proteins and larger peptides.

reproducibility than silica columns.

Analyte

Molecular Weight

Solubility in aqueous solutions

Difference between the compounds to be separated

Sample

Instrument and Application

Mobile Phase

Solution solven

or ASB C18; normal phase mode with aqueous mobile

phase.

pH

column before use!)

Salt concentration

Particle Size

Particle Size

Column Length

Column Diameter

small sample volume

analytical and mini-prep

HP

LC

Colu

mns


Bonna-Agela HPLC Column Selection Guide

Comparison of Technical Properties for C18 Phases

Unisol C18

Unisol C18(2)

Venusil AQ C18

Venusil ASB C18

Promosil C18

Waters SunFire C18

Waters Symmetry C18

Hypersil BDS C18

Hypersil ODS

Inertsil ODS

Inertsil ODS3

Supelco Discovery C18

Luna C18(2)

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

125

335

—

22

18

18

15

12

18

12

15.5

11

15

12

double

Single

double

yes

yes

double

no

double

no

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

—

no

no

no

yes

special

yes

yes

Stationary Phase Pore (Å) Surface (m2/g) Carbon load(%) End Capping ultra Pure Silica

ASB C18

ASB C8

Unisol C18

AQ C18

Bonshell

ASB C18

HILIC

Durashell

C18(L)

Amino

Cyano

HILIC

HILIC

Amino

Cyano

Silica

AQ C18

Unisol C18(2)

C18

C8

C1

Phenyl

Unisol C18

AQ C18

ASB C18

HILIC

Unisol C18(2)

Bonshell C18

Venusil CA

Venusil CO

Venusil CJ

Chiral Amide-1

Reversed Phase

Polar solventWater-soluble

Target CompoundFat- soluble

Organic Solvent

Normal Phase

Acid Base Hydrophilic Hydrophobic Chiral Antipode

Strong ionic Polar zwitterionicNeutral

HP

LC

Colu

mns


Best Value


Cross-reference for Bonna-Agela HPLC Columns

HP

LC

Colu

mns


HP

LC

Colu

mns


Best Value


HP

LC

Colu

mns


USP Column Selection Guide

L11

L12

L13

L15

Octadecyl silane (ODS or C18) chemically bonded

diameter.

An essentially monomolecular layer of amino-

propylsilane (NH2) chemically bonded to totally porous

Nitrile groups (CN) chemically bonded to porous silica

Phenyl groups chemically bonded to porous silica

bonding a quaternary amine to a solid silica spherical

Trimethylsilane (C1) chemically bonded to porous

Dimethylsilane (C2) chemically bonded to a totally

Octadecyl silane (ODS or C18) chemically bonded to

silica gel of a controlled surface porosity bonded to a

Silica gel of a controlled surface porosity bonded to a

Alumina of controlled surface porosity bonded to a

Octyl silane (C8) chemically bonded to porous silica

sphere

sphere

sphere

sphere

sphere

sphere

sphere

sphere

core-shell

sphere

sphere

shpere

sphere

sphere

sphere

sphere

sphere

sphere

sphere

Irregular

Irregular

sphere

sphere

sphere

sphere

Unisol C18

Venusil ASB C18

Durashell C18

Durashell C18(L)

Promosil C18

Bonshell C18

2

Promosil NH2

Drushell NH2

Venusil ASB Phenyl

Venusil ASB C1

Promosil Silica

Innoval Silica

Brand Brand

Brand

Brand

Brand

Brand

Brand

Brand

Brand

Brand

Figure Figure

Figure

Figure

Figure

Figure

Figure

Figure

Figure

Figure

5

5

5

5

5

5

5

5

5

5

5

sphere

sphere

sphere

sphere

Venusil ASB C8

Bonshell C8

Brand Figure

5

HP

LC

Colu

mns


Best Value


L21

L22

L23

L25

L18

polymethacrylate or polyacrylate gel with quaternary

A semi-rigid hydrophilic gel consisting of viny l

anionic and cationic water-soluble polymers. A

Amino (NH2) and Cyano (CN) groups chemically

diameter.

sphere

sphere

Venusil Diol

Brand

Brand

Figure

Figure

5

L28

L31

L32

L33

L35

L38

A multifunctional support which consists of a high

been bonded with anionic functionality in addition to a

conventional reversed-phase C8 functionality.

percentage by weight alumina-based polybutadiene

Ethyl silane chemically bonded to a totally porous

55 % divinyl benzene.

and processed to provide pH stabitlity.

hydrophilic (diol-type) molecular mono layer bonded

is a polymethacrylate gel.

water soluble samples.

porous spherical resin.

Irregular

Brand Figure

HP

LC

Colu

mns


IrregularVenusil CA

Brand Figure

5

Octylsilane and octadecylsilane groups chemically

functionality in addition to a reversed- phase C8

functionality.

in diameter.

layer of polybutadiene on to spherical porous zirconia

Multifunction resin with reversed-phase retention

2

is coated with quaternary ammonium functionalized

divinylbenzene.

sphere

sphere

sphere

Venusil CO

Venusil PFP

Brand

Brand

Brand

Figure

Figure

Figure

5

5

L51

L52

L53

L55

L58

diameter.

Propyl silane chemically bonded to totally porous silica

provide hydrophilic characteristics and pH stability

the surface of which has been covalently modified

Angstrom units and consisting of ethylvinylbenzene

coating composed of 85 nm diameter microbeads

HP

LC

Colu

mns


Best Value


1.European and American Pharmacopeia

column temperature: 35

Chromatogram of system suitability test

Acetylsalicylic acid Impurity Salicylic acid

1.2 Acetylsalicylic acid and its related compounds

1.1 Venusil AQ C18 HPLC Column Recommended in European Pharmacopoeia

O

O

O

OH

HP

LC

Colu

mns



Fluocinonide

1.4 The gemcitabine hydrochloride and its related compounds

2PO (adjust pH to 2.5 with H3PO )

Mobile Phase B: methanol

Time (min)

8-13

Mobile Phase A (%) Mobile Phase B (%)

3

gemcitabine hydrochloride

HP

LC

Colu

mns


Best Value


2PO

metronidazole hydrochloride


1.6 Arginine

2PO (pH =3.5 )

Arginine

HP

LC

Colu

mns



1.7 Mometasone furoate and its related compounds

column temperature: 35

mometasone furoate beclomethasone

1.8 Triamcinolone acetonide and its related compounds

Triamcinolone acetonide Triamcinolone

HP

LC

Colu

mns


Best Value


Mobile phase A (%)

25

1. 9 Simvastatin and its related compounds

1.10 Ambroxol Hydrochloride

Simvastatin

Time min Mobile phase B (%)


HP

LC

Colu

mns


Mobile Phase A (%)

1.11 Lovastatin

1.12 Methylprednisolone

Mobile phase: phosphate buffer (Add1.32 g NH PO

3PO

Detector: UV 238 nm

Column Temperature: 35

Time (min) Mobile Phase B (%)

System suitability test

HP

LC

Colu

mns


Best Value


Ibuprofen

Column: Innoval C18

Mobile Phase A (%)Time (min) Mobile Phase B (%)

Mobile Phase: Water (adjust pH to 2.5 with H3PO


Chromatogram of system suitability test Chromatogram of ibuprofen sample

HP

LC

Colu

mns


1.14 Gliclazide

Mobile phase: triethylamine: trifluoroacetic acid: acetonitrile: water =

Detector: UV 235 nm


3PO

Time (min)

2

15

35

55

Mobile Phase A (%)

85

Mobile Phase B (%)

15

2.5 5 5 12.5 15 22.5

HP

LC

Colu

mns


Best Value


121185321

Column: Unisol Amide

Mobile Phase:

2.1 Validamycin A

2.2 Decitabine

B: Acetonitrile

Time (min) B %


Mobile Phase:

2. HILIC Applications

HP

LC

Colu

mns


2.4 Ganciclovir


2PO


Columns: Unisol Amide

B: acetonitrile

Time (min) B %

2.6 Stachydrine hydrochloride (in Motherwort)


Mobile Phase:

Detector: ELSD

The method has adopted in Chinese Pharmacopoeia

111

HP

LC

Colu

mns


Best Value


2.7 Stachydrine hydrochloride

2.8 Ligusticum wallichii

2.9 Uracil


Detector: ELSD


Mobile Phase:

Detector: ELSD


Mobile Phase:

2PO


TR=2.811(uracil)

HP

LC

Colu

mns

112


2.10 Sulfocarbamide



Fig.a

Column: Brand W C18

Fig. b

Column: Brand W C18

Fig. c


S

H2N NH2

2.11 Arginine

13121185321

38

32

28

22

18

12

8

2

-2


Mobile Phase:

2PO

113

HP

LC

Colu

mns


Best Value


LS

-

8.122

8.51

5.21

82

LS

U

Minutes

8.

1513121185321

2.12 Mannitol


Mobile Phase: water

Detector: ELSD


Mobile Phase:

Detector: ELSD

2.14 Lactose


Mobile Phase:

Detector: ELSD

HP

LC

Colu

mns


mi n1 2 3 5

mA U

D AD 1 S ig =2 R ef =3 ( AG EL .D )

2.3

3.3

5.5


Mobile Phase

OAc


Time (min) B%

2.16 Simple lipids


Mobile Phase:

2PO

-Na2HPO


2.17 Water-soluble vitamins

Moblie Phase:

5 15 25

25

125

VWD (

1.8

32

3 5 2

RT=1

115

HP

LC

Colu

mns


Best Value


HO

NHH 2NH

N

S

O

HH

O

CH 3

CH 3

O

ON a

H

min2

mAU

-2

NH

N

S

OO

OHO

CH 3

H 2NH HH

min5 15

mAU

Cefradine

Detector: UV 225 nm

Mobile Phase: Phosphate buffer

%

min2.5 5 5 12.5 15

mAU

Benzylpenicillin Sodium

HP

LC

Colu

mns


N

S

NH

H2N

N

O

OCH3

N

SHH

O

OO Na

OC H3

O

min2 8 12

Norm.

Mobile Phase: phosphate buffer

2PO 2HPO

min2

mAU

,H 2ONH

N

S

OO

OHO

Cl

H 2NH HH

min2 8 12

mAU

Cefaclor

NH

N

S

OO

OHO

CH3

H2NH HH

2O

min2 8

Norm.

n

HP

LC

Colu

mns


Best Value


with H3PO

min2.5 5 5 12.5 15

mAU

Sulbactam Sodium

Cefoperazone Sodium

O

N

NaO

H

S

OO

CH3

CH3

H

O

mAU

O

OH

HO

O

O

O

O

O

O

O OH

O O

HO

N

N

3PO

52 53 55-1

1

2

3

5

8

11.2

15

52 Detector: UV 232 nm

HP

LC

Colu

mns

118


Mobile Phase: H3PO

4.Synthetic Antimicrobial Agents

mi n2. 5 5 5 12 .5

mAU

Norf lo cin

N

N

H 3C

N

O

F

O

CO OH

H

CH 3

min2 8

mAU

Mobile Phase: NH

HN

N N

O

F CO OH

min1 2 3 5

mAU

25

125

with TFA):ACN=82:28

HP

LC

Colu

mns


Best Value


N=

As=1.3

15 25

min5 15

mAU

Rifampicin

Detector: UV 325 nm

2PO

2PO

min1 2 3 5

mAU

Fluconazole

H N

N N

O H

OO

O

F

H Cl

H3C O

O

H3C

O H

O

O

NN

NC H3

O HO H

H3C

O H

OC H3

H3C

O N H

C H3

C H3

O H

C H3

OH3C

HP

LC

Colu

mns


HN

N N

N

OOH

OH

H2N

O

min2 8 12

Norm.

Ganciclovir

Detector: UV 252 nm

121

HP

LC

Colu

mns


Best Value


O

CH3 H

CH3HO

H

HH

OH

OOH

min1 2 3 5

Norm.

Hydrocortisone

mi n2 8

No rm .

25

12 5

15

5

6.4 Dexamethasone

with TFA):ACN=82:28O

H

C H3

O H

O

O H

H3C

H OH

C H3

F

H

6.1 Ethinylestradiol

6. Steriod Hormones

HO

HH

C

OHCH3

H

CH

min1 2 3

Norm.

Ethinylestradiol

Detector: UV 281 nm

min2 8

Norm.

Triamcinolone Acetonide

6.2 Triamcinolone Acetonide

O

C H3

F

O HH

H

H

H

O

O C H3

C H3H O O

C H3

HP

LC

Colu

mns

122


min2 8

Norm.

Levonorgestrel

C C HO HH3C

HH

H H

O

O

HH

OH

HCH3

O OH

CH3O

min2 8 12

Norm.

Prednisone

O

O

CH3HO

CH3

FH

H

H

Cl

O

CH3

CH3

O

H

min2 8

Norm.

Clobetasol Propionate

6.6 Clobetasol Propionate

mAU

1 2 3 5 in

6.7 Fluocinonide

O

HF

C H3

F

C H3

H

H

O H

H

O

O

O

C H3

O

O

C H3

C H3

H

123

HP

LC

Colu

mns


Best Value


ON

S

HN

HN

CH3

NO2

H3C

CH3 HCl

min1 2 3 5

mAU

Ranitidine Hydrochloride

mi n1 2 3 5

mAU

Cimetidine

min2 8

mAU

25

125

Omeprazole

7.1 Ranitidine Hydrochloride

7. Medicine for Gastric Ulcer

7.2 Cimetidine

285:115

Mobile Phase: MeOH:Water:H3PO :TFA

Mobile Phase: NaH2PO

3PO

H N

N

C H3

S

HN

NC H3

HN

C H3

N

NH

S

O N

C H3

O CH3

C H3

OC H3

HP

LC

Colu

mns


AU

mAU

15 25 min

Ephedrina hydrochloridum

min5 15 25

mAU

5

15

25

Ephedrina hydrochloridum

min5 15 25

mAU

-2.5

2.5

5

5

12.5

15

5

8. Analysis of Alkaloids

8.1 Quaternary Ammonium Alkaloids from Coptidis

8.2 Matrine

Temperature: 25

2

Mobile Phase: ACN:Ethanol:3 % H3PO

3PO

125

HP

LC

Colu

mns


Best Value


N

O

O

+

CH3O

CH3O

Cl- H2O2

min2.5 5 5 12.5 15 5

mAU

Berberine hydrochloride

Berberine hydrochloride

min2.5 5 5 12.5 15 5

mAU

mesaconitineaconitine

hpyaconitine

min

mAU

-5

min2.5 5 5 12.5

mAU

Codeine phosphate

Morphine

with TEA=15:85

Detector: UV 235 nm

Mobile Phase: MeOH: CH3

adjust pH to 3.5 with Acetic acid)=15:85

Detector: UV 238 nm

8.4 Berberine Hydrochloride From Phellodendron

8.6 Berberine from Phellodendron

HP

LC

Colu

mns


Prochlora Diethofencar Triadimenol

Mobile Phase: A: Ammonium acetate buffer; B:ACN

Detector: UV 225 nm

Temperature: 25

Detector: UV 225 nm

Temperature: 25

Temperature: 25

A

1

AU

1

2

3

5

8

2. 8. 12.

AU

2

8

1.

1. 2

2. 8. 12 18

AU

1. 2. 3. 5. 8.

9. Agricultural Chemical

9.1 Analysis of 14 Herbicides

9.2 Haloxyfop-P-methyl

HP

LC

Colu

mns


Best Value


3

Gradient:

Sample: PTC-AA (derivatization of amino acids with PITC)

Time(min)

%B

11

12 15

10. Analysis of Amino Acids

10.1 Standards of Amino Acids

10.2 Amino Acids from Salmon Calcitonin

HP

LC

Colu

mns

128


Sample

Standards of Amino Acids

Pseudoephedrine

1.2 1.8 2.2 2.8 3.2Time.min

2.12

1.2e5

1.8e5

2.2e5

2.8e5

Lignocaine

.8 1. .2 1. 1.82 2.22 2. .8 3. .2Time.min

1.5e5

2.5e5

3.5e5

Mobile Phase: A:1 % Formic Acid in Water; B:Methanol

Temperature: 25

11. Applications in LC-MS

11.1 Pseudoephedrine for Plasma

HP

LC

Colu

mns


Best Value


Oleic acid

OH

O

51 1.52 2.53 3. 55 5.

Inte

nsity.

cps

Time.min

51 1.52 2.53 3. 55 5.

Inte

nsity.

cps

Time.min

51 1.52 2.53 3. 55 5.

Inte

nsity.

cps

Time.min

Sample: Prepared sample by SPE (Cleanert PEP)

Temperature: 25

11.2 Oleic Acid in Plasma

HP

LC

Colu

mns


Mobile Phase: MeOH:H3PO

N =7587

AS=1.03

N=82 49

As=1.0 0

12. Others

12.1 Glipizide

12.2 Terfenadine

Mobile Phase: MeOH:CH3 3COONH

Dopamine Isoprenaline

.D: UV Chromatogra nm

Intens.

mAU

5 15 25 35 Ti me mi

131

HP

LC

Colu

mns


Best Value


Mi nu te s

5 15 25 35

Vo

lts

1

2

3

5

Pe

rcen

t

85

1

2

Mi nu te s

2. 5 5. 5 12 .5 15 .5 22 .5 25 .5

Vo

lts

15

Pe

rcent

85

1

2

3

Detector: UV 215 nm

3PO

Mobile Phase: MeOH:CH3COONH

12.4 Organic Acids

12.6 Saccharin Sodium in Milk Powder

Standard

CH3

12.7 DHA in the Health Food

HP

LC

Colu

mns

132


Mobile Phase: A: Ammonium acetate

12.8 Tartrazine in Food

12.9 Melamine in Feed

2

8

12

12 l

mA

3PO

Temperature: 25

12.10 Tetrodotoxin(TTX)

12.11 Baicalin

O

O

C H2O H

H

O HH

H

H O

NH

NH

H

O-

H O

H N H

O H

H

.5 5. .5 12.51 5 52 22.52 5 53

25

O

OO H

H O

O

OO H

O H

O HO

H O

133

HP

LC

Colu

mns


Best Value


Conditions:

B: Acetonitrile

Conditions:

15

25

Time(min) A (%) B (%)

2 8 12

VWD (

3

.8

15

5

.5

52

Target

Impurity

HP

LC

Colu

mns


Procedures for Column Regeneration

Due to interactions between the stationary phase and

require cleaning or regeneration. The following conditions

apply to silica-based columns. Flow rates should be

equation:

2V = Column Volume in mL

r = Column Radius in cm

L = Column Length in cm

UNBONDED SILICA COLUMNS (SILICA)

REVERSED PHASE COLUMNS

2)

when used in reversed phase mode:

3. Re-equilibrate to Mobile phase conditions.

5 Column Volumes of Water

5 Column Volumes of Water

For protein removal

Guanidine Thiocyanate

REVERSED PHASE PROTEIN/PEPTIDE

BONDED NORMAL PHASE COLUMNS

2 and DIOL)

GFC/SEC COLUMNS FOR PROTEINS

ION-EXCHANGE COLUMNS

2 and DEAE)

135

HP

LC

Colu

mns


Best Value


Methods of Maintaining Good Column Lifetime and Performance

Flush columns frequently using an appropriate program

Remove unstable and strongly retained components of no-interest from samples

Avoid elevated temperature unless it is necessary

Wash out all buffer salts and store the columns in high organic solution for overnight or long time storage

Agle hplc2014

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