Aggrecan and its role in bone development
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Transcript of Aggrecan and its role in bone development
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Aggrecan and its role in bone development
Aryn Gabai
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Role of Aggrecan• Very large (200+ kDal) structural protein• Links ECM components, compression resistant
– essentially a ECM scaffold• Connects hyaluronic acid and link protein for
bone mineralization• We have observed that PERK mice have a
reduced amount of aggrecan in the total growth plate
• Difficulty with identifying causes for reduced amount – smaller gp? less aggrecan? reduced chondrocytes?
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7289 WT 19d
7289 WT 19d
7293 KO 19d
7296 KO 19d
7297 WT 19d
7296 KO 19d12916 HT 17d
12915 WT 17d
12913 KO 17d
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• Isolated aggrecan for REX chondrocytes
• Shows a working aggrecan western
------- 5 uL-------- ------ 10 uL-------
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15d HT
15 d
WT
15d HT
15d KO
15d HT
15d HT
15d KO
15d WT
17d KO
17d HT
17d HT
REX
• Tissue-based westerns are not as clear or quantifiable
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Project Future
• Is this data enough to continue researching this protein?
• Identify housekeeping protein to quantify western results
• More western replication