Agarose bead

Protein A(Binds Fc of IgG)

Anti-P35 Ab

P35

Cdk5

140,000

125,000

43,000

Affinity Chromatography- Pull-down assay

AVP anti-viral product

DAI Double-stranded RNA-activated inhibitor of translation.

2’-5’-oligo-adenylate synthase dsRNA-dependent protein kinase

Viral sensing. In the cytoplasm, viral dsRNA or ssRNA containing 5'-triphosphate (ppp) is specifically recognized by RIG-I, initiating interferon production. In some cells, free viral nucleic acids, including those released from infected and killed cells, can be internalized by endocytosis and detected by Toll-like receptors in endosomes, also leading to interferon production. These mechanisms may cooperate to detect invading viruses and thence to initiate immune responses.

Science 2006, 314 p935-936.

Activated macrophages

Mycobacterial products

Latex-OVA orOvalbumin peptides

Endocytosis

Antigen processing and presentation

MHC-I restrictedCD8OVA1.3

MHC-II restrictedDOBW

MHC-IAntigen

TCRMHC-II

IL-2

IL-2

Antigen processing and presentation Assay.(Tobian A et al., 2003 J.Immunol 171 p1413.)

phycoerythrin allophycocyanin

(fluorescein isothiocyanate) (R-PE:cyanine-5)

Proteins of E. coli.

Protein with the tag of 6 neighboring histidine residues

Metal-affinity chromatography matrix:nickel-nitriloacetic acid