Guvernul Romaniei · 2017. 7. 13. · Created Date: 7/13/2017 1:18:57 PM
Administrative details - Guvernul Romaniei...Stefan N. Constantinescu, Therapy Targets in...
6
11/03/2013 1 Cristiana Tanase, Radu Albulescu Victor Babes National Institute of Pathology Administrative details Project title: Implementing proteomics technologies for the discovery of novel cancer biomarkers / CANBIOPROT Project No. ID 685 / Cod SMIS-CSNR 12644/152/11.06.2010 Funding agency: NASR, Structural funds, POS-CCE 2.1.2 Duration: 36 month, June 2010-June 2013 Total value: 5.271.786 lei; FEDR – 4.051.728 lei, State budget – 829.872 lei
Transcript of Administrative details - Guvernul Romaniei...Stefan N. Constantinescu, Therapy Targets in...
11/03/2013
1
Cristiana Tanase, Radu Albulescu
Victor Babes National Institute of Pathology
Administrative details Project title: Implementing proteomics technologies
for the discovery of novel cancer biomarkers / CANBIOPROT
Project No. ID 685 / Cod SMIS-CSNR12644/152/11.06.2010
Funding agency: NASR, Structural funds, POS-CCE2.1.2
Duration: 36 month, June 2010-June 2013
Total value: 5.271.786 lei; FEDR – 4.051.728 lei,
State budget – 829.872 lei
11/03/2013
2
Partner: INCD Victor Babes Beneficiary: INCD Victor Babes
Project director: Stefan N. Constantinescu, Ludwig Institute, Burussels, Be
Objectives Increasing skills and capabilities in proteomics,
focusing in method development and implementation of proteomics methods aiming at the discovery, validation and application of novel biomarkers in cancer diagnostics
Focus on major killers:
Glioblastoma
Cervical cancer
Pancreatic cancer
11/03/2013
3
Methodological background Standardization of pre-analytical and analytical
procedures
Proteomics technologies involved:
Mass spectrometry (SELDI-TOF, LC-MS)
2D Electrophoresis + 2D – DIGE
Multiplex – xMAP
Protein arrays
Bioinformatics and biostatistics tools
Flow chartREZULTATE
VVaalliiddaarree sseettddee bbiioommaarrkkeerrii
Implementarea tehnologiilor proteomice
BBrreevveettaarree
SSeett bbiioommaarrkkeerrii
Ser/plasma/tesut/supernatantCulturi celulare
FAZA IIFAZA I
DDeessccooppeerriirreeBBiioommaarrkkeerrii
CCaalliiffiiccaarreeVVaalliiddaarree
BBiioommaarrkkeerriiCCaannddiiddaattii
Tehnici de imuno-detectie
Western blotxMAP
Protein-microarray
SELDI-ToF-MS 2D, DIGE
Ser/plasma/tesut/supernatantCulturi celulare
Profil proteic/peptidic Citokine, factori de crestere, molecule de semnalizare
11/03/2013
4
Achievements so far:
Human resources: 5 position for doctoral and post-doctoral fellows in the project
Development of infrastructure
Partnership with Biomics Center – St. George University – London
Identification of sets potential diagnostic biomarkers for Glioblastoma, PDAC and cervical cancers (under validation), up to identification of several individual candidates
1 BOOK and 1 book chapter
3 publications in high impact journals
4 Submissions under evaluation
3 patent applications
Acquisition of research equipment
xCellingence
2D-DIGE
11/03/2013
5
Books:
Tănase C., C. Badiu, I. Ogrezeanu, Molecular Pathology of Pituitary, ISBN: 978-0-12-415830-6, Elsevier, 2011, DOI:10.1016/B978-0-12-415830-6.00001-9
Tănase C., Neagu M., Albulescu R., Hinescu M.E., Advances in pancreatic cancer detection, Advances in clinical chemistry, edited by Gregory S. Makowski, Ed. ELSEVIER, 51, 145-180 (2010).
Scientific articles
Maria Linda Cruceru, Monica Neagu, Jean-Baptiste Demoulin, and Stefan N. Constantinescu, Therapy Targets in Gliobastoma and Cancer Stem Cells: Lessons from Hematopoietic Neoplasms, JCMM, 2013 in press
Maria Linda Cruceru, Ana-Maria Enciu, Adrian Claudiu Popa, Monica Neagu, Radu Albulescu, Cristiana Pistol Tanase, Stefan N. Constantinescu, Signal Transduction Signature in Glioblastoma: Multiplex Analysis and Real Time Cell Assays, Current molecular Medicine, 2013, in press
Tanase Cristiana, Codrici Elena, Popescu Ionela Daniela, CruceruMaria Linda, Enciu Ana Maria, Albulescu Radu, Ciubotaru Vasile, Arsene Dorel, ANGIOGENIC MARKERS: MOLECULAR TARGETS IN PITUITARY ADENOMA,Personalized Medicine,2013,in press
Radu Albulescu, Elena Codrici, Ionela Daniela Popescu, Simona Mihai, Laura Georgiana Necula, Daniel Petrescu, Mihaela Teodoru , CristianaPistol Tanase, Cytokine Patterns in Brain Tumour Progression, Mediators of Inflammation, 2013 in press
11/03/2013
6
Scientific articles
Dima SO, Tănase C., et al, An Exploratory Study of Inflammatory Cytokines as Prognostic Biomarkers in Patients With Ductal Pancreatic Adenocarcinoma., Pancreas, 2012, 41(7): 1001-1007, PANCREAS
Albulescu R, Neagu M, Albulescu L, Tănase C., Tissularand soluble miRNAs for diagnostic and therapy improvement in digestive tract cancers. Expert Rev Mol Diagn. 2011; 11(1):101-20.
Tanase, Cristiana Pistol; Albulescu, Radu; Neagu, Monica, Application of 3D hydrogel microarrays in molecular diagnostics: advantages and limitations, Expert Review of Molecular Diagnostics, 2011, Volume: 11, Issue: 5, Pages: 461-464
Thank you for your attention!
