Adjuvant effect and TLR4 mediated activation of

macrophages by polysaccharide from Polyporus albicans

Author: Antik Bose

Senior Scientist and Project Advisor, Fred Hutchinson Cancer Research

Center, 1100 Fairview Avenue N, Seattle, WA 98109.

Abstract

A water soluble polysaccharide from mycelium of Polyporus albicans has been

identified for its potency in stimulating cellular and humoral immune response.

The adjuvant potential of this polysaccharide was identified and found to be

higher than conventional adjuvants. The 37 KDa polysaccharide was purified using

different macroporous absorption resins and Reverse phase HPLC. Structural

determination was done by sugar and methylation analysis, 13C NMR, FTIR,

MALDI-TOF,ESI-IT, MALDI-PSD TOF spectroscopy. The molecular size of the

polysaccharide was determined by Zetasier nanosystem. It showed TLR4

dependent NO, IL-1β and TNF-α production.