Adenoid Cystic Carcinoma: A clinical and molecular review
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Adenoid Cystic Carcinoma: A clinical and molecular reviewPatrick Ha, MD FACSAssociate Professor, Johns Hopkins Department of OtolaryngologyJohns Hopkins Head and Neck SurgeryGBMC Head and Neck Grand Rounds, November 1, 2013
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Disclosures
• Research Funding: – NIH/NIDCR – Champions Oncology– Adenoid Cystic Carcinoma Research
Foundation
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Adenoid cystic carcinoma
Of all tumors in the region of the head and neck, the adenoid cystic carcinoma is one of the most biologically deceptive and frustrating in management. The subtlety of its presence, masquerading often as a benign tumor for years, the unexpected pernicious extensions, the high incidence of local recurrence and systemic spread, and the temporary favorable response to irradiation usually lead on the pathway to death.
- John Conley
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Adenoid Cystic Carcinoma
• Second most common salivary gland malignancy
• Age: 40-50• Women > men 3:2• No associations with known exposures or
diseases• 50% present with pain• Minor salivary glands > submandibular > parotid
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Numbers
• Disease free survival: 60%, 50% 45%• Cause specific survival: 80%, 65%, 50%,
40%• 25-50% metastatic rate• Time to metastasis: 36 months (1mo – 19yrs)• Survival after metastasis: 40%, 15%, 10%
(isolated pulmonary metastasis > bony mets)
Spiro RH, Am J Surg 1997;174(5):495-8
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Pathology• Cribriform (swiss
cheese)• Tubular• Solid
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Surgical approach
• Wide local excision• Neck dissection (?)• Clear margins• Balance with functional outcome• Nerve preservation when possible• Metastatectomy
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Surgical approach
• “the biggest operation that can be rationally developed is the best” (Conley 1974)
• Casler and Conley (1992) espoused radical parotidectomy for T2-3 lesions
• No difference in survival with radical surgery• Obtain clear margins when feasible, balance
with functional outcome• Elective lymph node sampling low yield
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Metastatectomy
• Locati et al (2005) performed 26 procedures
• If clear margins, then improved freedom from progression
• No benefit to survival shown• Liu et al (1999) – pulmonary resection
did not cure patients• Still performed in some centers
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Peter Tork
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Radiotherapy
• No prospective randomized controlled study• Doses >60Gy more effective• Not effective as primary treatment (may have
palliative role)• Appears to be good for local control in
adjuvant setting• ?effect on survival• Retrospective bias
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Garden AS et al, Int J Radiation Oncol Biol Phys 1995
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Garden AS et al, Int J Radiation Oncol Biol Phys 1995
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Neutron Beam Therapy
• Reduced cell damage repair, less variation during cell cycle, less oxygen requirements
• 1988 – RTOG study found neutron beam better at local control and trend towards survival1
• Douglas et al2 – 159 patients with unresectable or gross+ margins. 5 yr survival 77%.
1Laramore GE et al. Int J Radiat Oncol Biol Phys. 1993;27(2):235-402Douglas JG et al, Int J Radiat Oncol Biol Phys. 2000;46(3):551-57
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Laramore et al. Int. J. Radiat. Biol. Phys. 27: 235-240, 1993
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Their conclusion
• “Further improvements in local-regional control are not likely to impact survival until more effective systemic agents are developed to prevent and/or treat distant metastatic disease.”
2Douglas JG et al, Int J Radiat Oncol Biol Phys. 2000
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Huber PE et al. Radiotherapy and Oncol 2001;59(2);161-67
Neutron therapy and ACC
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Neutron therapy and ACC
Huber PE et al. Radiotherapy and Oncol 2001;59(2);161-67
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• Neutron beam less used recently• Follow up studies not as favorable• Toxicity level much higher• Investigators looking at carbon ions (Phase II)
• ACCEPT - Cetuximab, IMRT with C12 heavy ion boost) for recurrent disease.
• COSMIC – IMRT with C12 boost
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Adam Yauch (MCA)
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Role for Chemotherapy in ACC
• ACC needs systemic control• Many limited trials performed• Difficult to quantify response• Limited numbers of patients – can one
use the same agents across histologies?
• Chemotherapy versus targeted therapy
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Important Guidelines in ACCTrial Evaluation
• Evaluate rationale for use of known drugs in ACC: molecular basis for therapy
• Enrollment criteria – recurrent vs progressive vs metastatic
• Adherence to RECIST criteria reporting
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Single Agent Chemotherapy Trials
Papaspyrou et al, Head Neck, 2011:33:905-11
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Molecular targets in ACC
• cKIT – growth, differentiation and migration. Expressed highly in 100% of ACC. No mutations found. May not be phosphorylated (activated) in ACC
• EGFR – proliferation, motility, adhesion, invasion, angiogenesis, survival. 0-85% expression in ACC
• Her-2 – 0-100% in ACC.
Liu J et al, Head Neck, 2011
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More molecular targets…
• VEGFR – angiogenesis. Independent prognostic factor in salivary gland carcinoma. Cell line studies performed*
• NFkB – suppresses apoptosis, regulated VEGF - ubiquitin-proteasome degradation pathway.
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The c-kit story
• Identified as a tyrosine kinase receptor in 1987
• Proto-oncogene, found in testicular tumors, AML, GI stromal tumors, ACC
• Found in a high percentage of ACC (Jeng et al, 2000, Freier et al 2005)
• Loss of function: deafness, pigment defects
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C-Kit Pathway
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Imatinib
• Blocks abl, c-kit, PDGF-R• Imatinib used in 2 patients with unresectable
disease with + response (Alcedo et al 2004)• Formal multicenter trial – 16 patients with
unresectable disease (Hotte et al, 2005)• No objective measures of response• 9/16 patients with stable disease• 6 patients with progressive disease• Separate trial with 10 patients also closed
(Pfeffer et al, 2006)
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Lapatinib
• ErbB2 and EGFR inhibitor• Recurrent, progressive, or metastatic
disease tested for 1+ EGFR and/or 2+ ErbB2
• 29/33 patients met criteria, 19 assessable patients treated
• No objective response, 15 with stable disease (9 with stable disease >6 months), 4 with progressive disease.
Agulnik M et al, JCO 2007 25(25):3978-84)
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ECOG 1303: Bortezomib + doxorubicin
• Bortezomib – inhibitor of NFkB and 26S proteasome
• If progressed, added doxorubicin• 24 patients received tx, no objective
response with bortezomib – stable dz in 15/21. 10 had both D+B, 1 partial response, 6 stable disease
• Well tolerated, but no response notedArgiris A et al, Cancer 2011, Aug 1; 117(15):3374-82
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Sunitinib
• Target VEGF, c-kit, RET, FLT3• Progressive, recurrent and/or metastatic
ACC• 13 assessable patients – no objective
response. 11 with stable disease (8>6 months), 2 patients had progression.
Chau NG et al. Ann Oncol 2011
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Cetuximab and Cis-platinum
• Locally advanced* (n=9) or metastatic (n=12) with positive EGFR expression
• Loaded with Cetuximab, followed by weekly infusion
• Locally advanced tumors received radiation and cisplatin
• Metastatic received cis-platin and 5-FU
Hitre E, et al. BJC 109, 2013
* Refused surgery or unresectable
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• Local advanced disease (n=9):– PFS = 64 months – 2-yr OS =100%– 2 CR, 2 PR, 5 SD
• Metastatic (n=14):– PFS = 13 months– 2-yr OS = 24 months– 5 cases of PR
Hitre E, et al. BJC 109, 2013
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Current Trials
• Eribulin Mesylate in recurrent or metastatic salivary gland cancer– Phase II. Microtubule inhibitor. Most
studies in breast cancer• RTOG 1008: radiation therapy +/- cis-
platin in advanced salivary gland cancers
• Dovitinib
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Ways to improve trials
• Consider symptom improvement• Include survival statistics• Include progressive or symptomatic
patients only and describe criteria• Consider previous therapies and
number/site of recurrence• Report ACC results separate from other
salivary tumorsLaurie SA et al, Lancet Oncology, 2011:12;815-24
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John McCain
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Myb-NFIB fusion gene
• NFIB – human nuclear transcription factor (9p23-24)
• MYB – oncogene – cell proliferation, apoptosis, differentiation. (6q22-23)
Persson et al, PNAS, 2009: 106(44): 18740-44
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Implications of Myb-NFIB Fusion Gene• Myb-NFIB fusion present in 28-49% of ACC• Very specific for ACC, not other SGTs• Majority of ACC showed Myb overexpression,
but higher levels if fusion present• Fusion may be variable• Unclear prognostic significance – high Myb
expression and/or fusion gene may suggest worse outcome
West RB et al, Am J Surg Pathol, 2011:35(1):92-9Mitani Y et al. Clin Cancer Res, 2010;16(19):4722-31
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MYB
• No drug specifically targeting MYB yet• Possible downstream targets:
– Cell cycle control (CCNB1, CDC2, MAD1L1)
– Apoptosis (API5, BCL2, BIRC3, SHPA8, SET)
– Cell growth/angiogenesis (MYC, KIT, VEGFA, FGF2, CD53)
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Exome Sequencing
• Low mutational rate• Confirmed Myb translocations• Implicated FGFR pathway (3/24 and
30%), chromatin regulation • Underwhelming overall
Ho AS et al. Nature Genetics, 2013Stephens PJ et al, JCI, 2013
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Dovitinib
• FGFR1 is over-expressed (1a) and phosphorylated (1b) in the preponderance of ACC tumors.
• FGF2, a molecule that binds to FGFR1, is a known downstream target of MYB
• Screened in several xenograft mouse models of ACC and demonstrated activity
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Dovitinib Trial
• Recurrent or metastatic disease• Phase II – endpoint = survival• Orally dosed – 500 mg, 5 days on, 2
days off for 4 weeks.• Treatment continues• 10 accrued in Uva, open enrollment for
33 in Korea
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Other Possibilities?
• Personalized medicine approaches– Identification of specific known tumor
markers– Xenograft model creation
• In vivo testing for predictive tumor behavior• Identification of novel personal markers
• Molecular profiling for identification of newer drug-able targets
Moskaluk CA et al, Lab Invest, 2011:91(10);1480-90
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Conclusions
• Difficult tumor to treat when recurrent or metastatic, which is common
• Surgery + radiation are standard• Numerous clinical trials, but limited
conclusions drawn• Supports enrollment in clinical trials• Hope for molecular discovery leading to
newer rational targets
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Irwin Jacobs
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ACCRF