Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

11
Activation 0f S- phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1 ) Active (E2F)

description

Active (E2F). Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1). Strategies for signalling inhibition. Immune effector cell. Anti-ligand mAbs. Bispecific Abs. scFv/toxin conjugates. Ligand/toxin conjugate. Ligand- genistein conjugates. Anti-receptor mAbs. - PowerPoint PPT Presentation

Transcript of Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

Page 1: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

Activation 0f S-phase genes(Cycle E, A, E2Fs,

DHFR,TK,TS,POL,CDK1)

Active (E2F)

Page 2: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 3: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 4: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 5: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 6: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 7: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 8: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

Strategies for signalling inhibition

Tyrosine kinase inhibitors (TKIs)

Immuneeffector cell

Anti-ligand mAbs

BispecificAbs

Anti-receptormAbs

Ligand/toxin conjugate

scFv/toxinconjugates

Ligand-genisteinconjugates

IntracellularscFvs

Nucleus

Antisense

Inhibitors of other signalling molecules

Page 9: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

DNA

Mode of action of EGFR inhibitors

Membrane

Extracellular

Intracellular

R

K

R

K EGFR-TKIEGFR-TKI Signalling

Proliferation Cell survival (anti-apoptosis)

Growth factors

Chemotherapy/radiotherapy sensitivity

Angiogenesis

Metastasis

R, epidermal growth factor receptor

EGF/TGFα

Antibody

Page 10: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)
Page 11: Activation 0f S-phase genes(Cycle E, A, E2Fs, DHFR,TK,TS,POL,CDK1)

Optochemical Control of Protein Localization and Activity ... · biochemically active, we tested whether Haloenzyme and DHFR could be chemically and optically dimerized in vitro.

Optochemical Control of Protein Localization and Activity ... · biochemically active, we tested whether Haloenzyme and DHFR could be chemically and optically dimerized in vitro.

Deletion Analysis of the Chinese Hamster … · indirect assay for cellular DHFR activity that could prove useful for gene transfer analysis where dhfr is a reporter gene. MATERIALS

Deletion Analysis of the Chinese Hamster … · indirect assay for cellular DHFR activity that could prove useful for gene transfer analysis where dhfr is a reporter gene. MATERIALS

Cyclin B–Cdk1 activates its own pump to get into the nucleus...Cyclin B–Cdk1 activates its own pump to get into the nucleus • Lindqvist 199 Masui, Y., and C.L. Markert. 1971.

Cyclin B–Cdk1 activates its own pump to get into the nucleus...Cyclin B–Cdk1 activates its own pump to get into the nucleus • Lindqvist 199 Masui, Y., and C.L. Markert. 1971.

RB and E2Fs - UiO

RB and E2Fs - UiO

Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter ...

Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter ...

CDK1 substitutes for mTOR kinase to activate mitotic … · CDK1 substitutes for mTOR kinase to activate mitotic cap-dependent protein translation Masahiro Shuda, ... kinase 1/cyclin

CDK1 substitutes for mTOR kinase to activate mitotic … · CDK1 substitutes for mTOR kinase to activate mitotic cap-dependent protein translation Masahiro Shuda, ... kinase 1/cyclin

Dynamics and dissipation in enzyme catalysisch10/Papers/Miller_1.pdfcatalysts (22). Escherichia coli dihydrofolate reductase (DHFR) is an exten-sively studied prototype for protein

Dynamics and dissipation in enzyme catalysisch10/Papers/Miller_1.pdfcatalysts (22). Escherichia coli dihydrofolate reductase (DHFR) is an exten-sively studied prototype for protein

Targeting CDK1 and MEK/ERK Overcomes Apoptotic ......Cell Death and Survival Targeting CDK1 and MEK/ERK Overcomes Apoptotic Resistance in BRAF-Mutant Human Colorectal Cancer Peng Zhang1,

Targeting CDK1 and MEK/ERK Overcomes Apoptotic ......Cell Death and Survival Targeting CDK1 and MEK/ERK Overcomes Apoptotic Resistance in BRAF-Mutant Human Colorectal Cancer Peng Zhang1,

INTEGRATED ANALYSES OF THE OVARIAN CANCER GENOME CDK1 INHIBITION SENSITISES AND TUMOURS WITH WILD TYPE BRCA1 TO PARP INHIBITION  

INTEGRATED ANALYSES OF THE OVARIAN CANCER GENOME CDK1 INHIBITION SENSITISES AND TUMOURS WITH WILD TYPE BRCA1 TO PARP INHIBITION  

CDK1 Chromosome condensation Sister chromatid separation CDK2 DNA replication; Repair of damage APC DNA replication checkpoint Spindle checkpoint SG2MetaphaseAnaphase.

CDK1 Chromosome condensation Sister chromatid separation CDK2 DNA replication; Repair of damage APC DNA replication checkpoint Spindle checkpoint SG2MetaphaseAnaphase.

Overview of current and future targets of breast cancer medicines€¦ · as an alkylating agent, microtubulin inhibitor and Dihydrofolate Reductase (DHFR) thymidylate – synthetase

Overview of current and future targets of breast cancer medicines€¦ · as an alkylating agent, microtubulin inhibitor and Dihydrofolate Reductase (DHFR) thymidylate – synthetase

the DHFR gene alters the polyadenylation pattern of DHFR ...

the DHFR gene alters the polyadenylation pattern of DHFR ...

SnapShot: Adipocyte Life Cycle · at least three groups (1): cell-cycle regulators (e.g., p21, Cyclin D1, Rb, and E2Fs), hormones (e.g., thyroid and growth hormones), and angiogenic

SnapShot: Adipocyte Life Cycle · at least three groups (1): cell-cycle regulators (e.g., p21, Cyclin D1, Rb, and E2Fs), hormones (e.g., thyroid and growth hormones), and angiogenic

Cell cycle control in mammalian cell 5 ⋆ Phosphorylation of Rb protein prevents its association with E2Fs, thereby permitting E2Fs to activate transcription.

Cell cycle control in mammalian cell 5 ⋆ Phosphorylation of Rb protein prevents its association with E2Fs, thereby permitting E2Fs to activate transcription.

Mitotic events - early Import of cyclin B1-cdk1. Disassembly of the nuclear lamina. Chromosome condensation. Dissociation of cohesins.

Mitotic events - early Import of cyclin B1-cdk1. Disassembly of the nuclear lamina. Chromosome condensation. Dissociation of cohesins.

1 Gene amplification for high level recombinant protein production in mammalian cells. Principal system = dhfr- CHO cells Facilitated by the availability.

1 Gene amplification for high level recombinant protein production in mammalian cells. Principal system = dhfr- CHO cells Facilitated by the availability.

Southern Mozambique Europe PMC Funders Group . Author ...researchonline.lshtm.ac.uk/8221/1/Differential Effect of...dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps),

Southern Mozambique Europe PMC Funders Group . Author ...researchonline.lshtm.ac.uk/8221/1/Differential Effect of...dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps),

CDK1 substitutes for mTOR kinase to activate mitotic cap ... · dent translation compared with interphase cells. This appears to be due to cyclin-dependent kinase 1 (CDK1) activity

CDK1 substitutes for mTOR kinase to activate mitotic cap ... · dent translation compared with interphase cells. This appears to be due to cyclin-dependent kinase 1 (CDK1) activity

Localization of the Human Malaria Parasite's DHFR-TS and ...

Localization of the Human Malaria Parasite's DHFR-TS and ...

Carlos A. Machado*, Francisco J. Ayala · Sequence variation in the dihydrofolate reductase-thymidylate synthase (DHFR-TS) and trypanothione reductase (TR) genes of Trypanosoma cruzi

Carlos A. Machado*, Francisco J. Ayala · Sequence variation in the dihydrofolate reductase-thymidylate synthase (DHFR-TS) and trypanothione reductase (TR) genes of Trypanosoma cruzi