ACB Wakame Bioferment Advanced - Active Concepts,...
Transcript of ACB Wakame Bioferment Advanced - Active Concepts,...
ACB Wakame Bioferment Advanced
Tomorrow’s Vision… Today!®
Anti-Aging, Antioxidant
ACB Wakame Bioferment Advanced
*20024 – ACB Wakame Bioferement Advanced
Product Code: 20024
INCI Name: Undaria Pinnatifida Cell Culture Extract
INCI Status: Conforms
Suggested Use Level: 0.5 - 2.0%
Suggested Applications: Anti-Aging, Antioxidant
Anti-Aging: The next step in actives
Live Yeast Cell Derivatives (LYCD) used for wound healing & increasing ATP production
• Based on the works of George Sperti• 1939 – Realized Cell fractions stimulate
metabolic activity & stimulate cellular proliferation
Cell extracts can do more
Stop band-aiding the problem – Find a Solution!
Anti-Aging: The current view
Intrinsic ROS are more of a threat than extrinsic
Mitochondra are like engines that produce ROS as a byproduct of oxidative phosphorylation
Damage builds up overtime
Topical antioxidant address extrinsic ROS• Band-Aid the Solution
Oxidative Phosphorylation
Is a metabolic process that fuels the production of Adenosine Triphosphate (ATP)
- ATP is the major source of energy in our cells
Is an electron transport system- Transfer of electrons from donors to
receptors (oxygen)- Redox reactions are carried out by 5
mitochondrial protein complexes (Electron Transport Chain)
- As electrons pass through the chain, energy is generated that is used to make ATP
Oxidative Phosphorylation is like a bowling ball rolling down stairs –generates energy every time is hits a step
The bowling ball analogy
Mitochondrial Aging
During oxidative phosphorylation, oxygen (final electron receptor) is reduced to water
Leakages occur in the system- Generate hydroxyl & superoxide
radicals instead of water- Cause damage to the mitochondria &
mitochondrial DNA (mtDNA)
As cells divide, damaged mtDNA from old cells pass on to new cells
- Damage to mtDNA accelerates the pace of aging
• The conservation of mtDNA is so strict that genealogists use it to trace lineages
• mtDNA was used to trace human species back to the Mitochondrial eve (140,000 years ago)
mtDNA
Mitochondrial Aging: A vicious cycle
Oxidative damage inhibits
mitochondrial function
Increase in the production of ROS
Mitochondria ceases to generate sufficient levels of
energy
Cellular functions are decreased =
Aging
Mitochondrial aging
• The use of topical antioxidants doesn’t counteract mitochondrial ROS
• The solution is to address the source – our mitochondria
How to break the cycle
ACB Wakame Bioferment Advanced
Cultured Wakame Cells
- Exposed to UV stress
- Extraction of synergistically active components (Heat Shock Proteins or Heat Shock Elements) – HSP’s
The Solution to Cellular Longevity!
ACB Wakame Bioferment Advanced
Traditionally live cell derivatives are produced by exposing cells to an environmental stress – UV irradiation
- Our method – supplementing the growth mead used to culture wakame cells
- Allows us to produce a unique cell derivative that is capable of optimizing electron transport to reduce mitochondrial leakage (oxidative phosphorylation)
- This leakage results in the release of damaging free radicals & ROS that ultimately contribute to mitochondrial damage & aging
The Solution to Cellular Longevity!
ACB Wakame Bioferment Advanced
Stop intrinsic damage by enhancing oxidative phosphorylation
Replenish structural proteins- Cell extracts have been shown to stimulate structural proteins
& wound healing
Increases oxygen Uptake
Powerful Antioxidant
Increases Collagen Synthesis
Target Aging
Efficacy Data: in-vitro
15 Minute Oxygen Consumption Assay
Protocol
• Performed to assess the ability of ACBWakame Bioferment Advanced toincrease cellular oxygen consumption
• Results indicate that ACB WakameBioferment Advanced is capable of inducing changes in oxygen consumption to fibroblasts
• Compared to the untreated controlACB Wakame Bioferment Advancedincreased oxygen consumption by26.8%
Figure 1: Results of the oxygen consumption assay.
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Untreated Contorl LYCD ACB Yogurt DermalRespiratory Factor
ACB WakameBioferment Advanced
Oxy
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Treated withFCCP
Efficacy Data: in-vitro
DNA MicroArray
Up regulated genes:
a) Structural proteins: Collagen, fibronectin, keratin
b) Oxidative phosphorylation
c) Mitochondrial genes
Down regulated genes:
a) Inflammation
b) ROS
c) NO2,000 genes up-regulated5,000 genes down-regulated
Efficacy Data: in-vitro
24 Hour Oxygen Consumption Assay
Protocol
• Performed to assess the ability of ACBWakame Bioferment Advanced toincrease cellular oxygen consumptionafter 24 hours of treatment
• Results indicate that ACB WakameBioferment Advanced is capable of inducing changes in oxygen consumption to fibroblasts
• Compared to the untreated controlACB Wakame Bioferment Advancedincreased oxygen consumption by26.8%
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Oxy
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Untreated Treated with ACB Wakame Bioferment Advanced
Figure 1: Results of the oxygen consumption assay.
Efficacy Data: in-vitro
ROS Formation Assay
Protocol
• Performed to assess the impact thatACB Wakame Bioferment Advancedhas on intracellular ROS formation
• Results indicate that ACB WakameBioferment Advanced is capable ofreducing ROS formation compared tothe untreated control
Figure 2: ROS Formation Results.
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Untreated 1% ACBWakame
BiofermentAdvanced
0.5% ACBWakame
BiofermentAdvanced
10ug/mLAscorbic
Acid
50ug/mLAscorbic
Acid
100ug/mLAscorbic
Acid
10uM H2O2 150uM H2O2 PBN Pretreat+ H2O2
AA Pretreat+ H2O2
Co
rre
cte
d R
FU
Time 0
Time 1hour
Efficacy Data: in-vitro
ORAC Assay
Figure 3: Antioxidant capacity of test materials compared to Trolox.
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100µM Trolox 12.5µM Trolox 0.1% ACB WakameBioferment Advanced
0.05% ACB WakameBioferment Advanced
An
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Cap
acit
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MTE
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Protocol
• Trolox® was used as the positive control
• Test Quantity: 0.05% & 0.1%
• Fluorescent measurements were taken every two minutes for two hours
• ACB Wakame BiofermentAdvanced showed antioxidant activity at levels as low as 0.05% concentration
Efficacy Data: in-vitro
NO Formation
Figure 4: NO Formation results shown in relative fluorescent units (RFU).
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Untreated 1% ACBWakame
BiofermentAdvanced
0.5% ACBWakame
BiofermentAdvanced
0.1% ACBWakame
BiofermentAdvanced
10 ug/mlAscorbic
Acid
50 ug/mlAscorbic
Acid
100 ug/mlAscorbic
Acid
10 uM H2O2
Ch
ange
in R
FU
Time 1hr
Time 6hr
Protocol
• Performed to assess the impact thatACB Wakame Bioferment Advancedhas on intracellular NO (nitric oxide)formation.
• ACB Wakame Bioferment Advancedwas able to reduce NO formation
Efficacy Data: in-vitro
Procollagen Assay
Figure 5: Comparison of the effects on procollagen levels following treatment.
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1%Magnesium
AscorbylPhosphate
0.5%Magnesium
AscorbylPhosphate
1% ACBWakame
BiofermentAdvanced
0.5% ACBWakame
BiofermentAdvanced
4mM SodiumButyrate
Untreated
Typ
e 1
C-P
ep
tid
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ng/
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Protocol
• An ELISA assay was used to assess ACB Wakame Bioferment Advanced impact on collagen synthesis.
• The results indicate that ACB WakameBioferment Advanced is capable ofincreasing the expression of type 1-CPeptide in a fibroblast cell culturemodel.
• Useful in cosmetic formulations intended to increase collagen synthesis
Efficacy Data: in-vitro
Figure 6: Comparison for percent increase in collagen I synthesis.
Protocol
• An ELISA assay was done to determine if ACB Wakame Bioferment Advanced is capable of inducing collagen I synthesis
• Three concentrations were compared toa positive and negative control.
• The findings indicate that ACB WakameBioferment Advanced is capable of increasing collagen I synthesis in a dose dependent fashion.
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Negative Control Positive Control 1% ACB WakameBiofermentAdvanced
2% ACB WakameBiofermentAdvanced
4% ACB WakameBiofermentAdvanced
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Collagen Synthesis
ACB Wakame Bioferment Advanced
*20024 – ACB Wakame Bioferement Advanced
Product Code: 20024
INCI Name: Undaria Pinnatifida Cell Culture Extract
INCI Status: Conforms
Suggested Use Level: 0.5 - 2.0%
Suggested Applications: Anti-Aging, Antioxidant
ACTIVE CONCEPTS LLC
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