AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

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© 2008 Applera Corporation and MDS Inc. MultiQuant Software © 2008 Applera Corporation and MDS Inc.

description

Intensity. Time. Cycle Time. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics. Supports RELATIVE and ABSOLUTE quantitation. Multiple MRM transitions to peptides processed as groups. User-defined report formats. - PowerPoint PPT Presentation

Transcript of AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

Page 1: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

Page 2: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

• Multiple MRM transitions to peptides processed as groups.

• Multiple MRM transitions to peptides processed as groups.

• User-defined report formats• User-defined report formats

Time

Inte

nsity

Cyc

le

Tim

e

• Supports RELATIVE and ABSOLUTE quantitation

• Supports RELATIVE and ABSOLUTE quantitation

Page 3: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

One touch productivity...One touch productivity...MultiQuant™ software incorporates many powerful features to convert the mountain of data into resultsMultiQuant™ software incorporates many powerful features to convert the mountain of data into resultsProteomics and Metabolomics researchers are increasingly turning to MRM quantitation for more targeted and reproducible results…Proteomics and Metabolomics researchers are increasingly turning to MRM quantitation for more targeted and reproducible results…

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

Page 4: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

Normal

Early stagedisease

Late stagedisease

ABSOLUTE: MRM peak areas are measured relative to synthetic internal standards to determine actual peptide and metabolite abundance.ABSOLUTE: MRM peak areas are measured relative to synthetic internal standards to determine actual peptide and metabolite abundance.RELATIVE: MRM peak areas for peptides are compared against a global internal standard, created using mTRAQ™ reagents.RELATIVE: MRM peak areas for peptides are compared against a global internal standard, created using mTRAQ™ reagents.MultiQuant™ software is a powerful solution that supports both RELATIVE and ABSOLUTE Quantitation workflows.MultiQuant™ software is a powerful solution that supports both RELATIVE and ABSOLUTE Quantitation workflows.

Endogenous Peptide

Internal Standard Peptide

R=0.9989

Standard Concentration Curve

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

Page 5: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

Information for processing standard concentration curves is at your fingertips.Information for processing standard concentration curves is at your fingertips.

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

MultiQuant™ software has many powerful features to enable quantitative workflows…MultiQuant™ software has many powerful features to enable quantitative workflows…

Page 6: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

When mTRAQ™ reagents are used, area ratios to internal standards for every peptide are automatically computed.When mTRAQ™ reagents are used, area ratios to internal standards for every peptide are automatically computed.Multiple MRM transitions to peptides are automatically processed as groups and peak integrations are easily visualized.Multiple MRM transitions to peptides are automatically processed as groups and peak integrations are easily visualized.

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

Results can easily be exported to user-defined report formats.Results can easily be exported to user-defined report formats.

Page 7: AB SCIEX Protein Research Solutions  for Targeted Quantitative Proteomics

© 2008 Applera Corporation and MDS Inc. MultiQuant™ Software© 2008 Applera Corporation and MDS Inc.

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

One touch productivity.One touch productivity.