System attributesAutomated workflowThe AB Library Builder™ System automates the most labor-intensive parts of library preparation, allowing you to reduce hands-on time by at least 50% compared to the manual workflow (Figure 1). When combined with the available library kits for Ion PGM™, Ion Proton™, SOLiD® 4, and 5500 Series systems, the AB Library Builder™ System is able to automate the steps from shearing to nick translation for fragment libraries, and the post-shearing and pre–nick translation portion for exome, whole-transcriptome, and targeted resequencing.

Scalable throughput The AB Library Builder™ System is designed to process up to 13 DNA or RNA libraries in a single run, and yield up to 39 libraries in a day. The system can prepare 13 DNA fragment

AB Library Builder™ SystemSimplified library construction for Ion Torrent, SOLiD® 4, and 5500 Series genetic analysis systems

The AB Library Builder™ System simplifies next-generation sequencing by providing automated library preparation for the Ion PGM™ Sequencer, Ion Proton™ Sequencer, and the SOLiD® 4 and 5500 Series genetic analysis systems. The system enables higher library throughput while reducing the hands-on time needed to prepare libraries for various applications such as exome, whole-transcriptome, targeted resequencing, and whole-genome sequencing.

Key benefits•Automated—enables reduction of

hands-on time by more than 50% compared to the manual workflow

•Scalable—prepares up to 13 libraries per run, up to 39 libraries per day

• Integrated—plug-and-play reagents and push-button protocol

•Flexible—single instrument for both library preparation and sample purification (using iPrep™ kits)

SPECIFICATION SHEETAB Library Builder™ System

libraries in about 6 hours (with less than 2 hours of hand-on time), whereas a manual library process could take nearly twice as long to prepare the same number of libraries (Figure 1).

Integrated solution The AB Library Builder™ System is an integrated solution comprising a predefined software protocol, plug-and-play reagents in cartridge format, and an instrument for nucleic acid purification and library preparation. Figure 2 illustrates the quality and yields of libraries prepared with the AB Library Builder™ System, which are comparable to those of manually prepared libraries. The AB Library Builder™ System is also fully compatible with all iPrep™ kits for DNA and RNA extraction, making it a fully automated sample preparation platform—from sample to library.

Method Recovery (µg)Pre-amplification

Amplifiable library (pmol)Post-amplification

Manual 0.36 0.90

AB Library Builder™ System 0.28 1.10

Figure 2. Comparison of manual and AB Library Builder™ System libraries. (A) Agilent Bioanalyzer® traces for comparable libraries constructed manually and using the AB Library Builder™ System. (B) Pre-amplification recovery and post-amplification library yields are comparable for both methods of library preparation.

Figure 1. Automated library preparation saves time and increases throughput. Hands-on and total time needed to prepare either 8 or 13 libraries manually versus using the AB Library Builder™ System is shown. Our tests show a >50% reduction in hands-on time for construction of most libraries.

Ordering information Cat. No.

System The AB Library Builder™ System includes: 4463794

•AB Library Builder™ instrument and power cords•Barcode reader and software•Protocol cards for the Ion PGM™/Proton™ Sequencers, SOLiD® 4, and 5500 Series Genetic Analyzer Systems •Accessories (racks, tubes, holders, etc.)•Service installation

AB Library Builder™ System with self-installation (not service installation) available in some regions 4463592

ConsumablesLibrary Builder™ Fragment Core Kit for SOLiD® 4: 13 prefilled reagent cartridges, enzymes, shearing buffer, Platinum® PCR amplification mix, tips, tip holders, and sample tubes 4463762

Library Builder™ Fragment Core Kit for 5500 SOLiD®: 13 prefilled reagent cartridges, enzymes, shearing buffer, Platinum® PCR amplification mix, tips, tip holders, and sample tubes 4463763

AB Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems 4472690SOLiD® Fragment Library Oligo Kit 4401151SOLiD® Fragment Library Barcoding Kit 1-96 44496375500 SOLiD® Fragment Library Standard Adaptors 44644115500 SOLiD® Fragment Library Barcode Adaptors 1-96 4464404Agencourt® AMPure® XP Kits (Beckman)

For more information on available Ion Torrent™ kits for the AB Library Builder™ system, visit lifetechnologies.com/ionproducts

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For Research Use Only. Not for use in diagnostic procedures. © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Bioanalyzer is a registered trademark of Agilent Technologies, Inc. Agencourt and AMPure are registered trademarks of Beckman Coulter, Inc. Printed in the USA. CO111956 1012

Visit lifetechnologies.com/ionprep to learn more about Ion Torrent™ sample preparation solutions, and lifetechnologies.com/solidlibraryprep to learn about library preparation solutions for the SOLiD® 4 and 5500 Series Genetic Analyzer Systems.

AB Library Builder™ System specifications

Library types DNA/RNA fragment libraries for the Ion PGM™/Ion Proton™ Sequencers, and SOLiD® 4 and 5500 Series Genetic Analyzer Systems

Throughput Up to 13 samples per run, up to 39 samples per day

Starting material 10 ng–5 μg sheared DNA, depending on platform and application

Instrument run time 2.5 hr

Multiplexing Up to 96 barcoded DNA fragment libraries

Dimensions and weight 50.8 cm x 55.9 cm x 57.2 cm (W x D x H), 55 kg

Operating environment Temperature: 5–40°C; maximum relative humidity: 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C

Power requirements AC 100–240 V, 240 VA, 50/60 Hz, grounding necessary

Manual

AB Library Builder™ System