A Zebrafish Holding Chamber for A Zebrafish Holding Chamber for Use with a Micro CT ScannerUse with a Micro CT Scanner

University of Wisconsin - Madison Biomedical Engineering Design Courses

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Team MembersTeam Members

Austin Ramme, Kristen Sipsma, Austin Ramme, Kristen Sipsma,

Andrew Neumann, and Mike HaggertyAndrew Neumann, and Mike Haggerty

ClientClient

Jill M. Kolesar, Pharm.D., BCPSJill M. Kolesar, Pharm.D., BCPSAssociate Professor of PharmacyAssociate Professor of Pharmacy

AdvisorAdvisor

Professor Justin WilliamsProfessor Justin Williams

Presentation OutlinePresentation Outline

Problem StatementProblem Statement Background InformationBackground Information Current Devices Current Devices Design SpecificationsDesign Specifications Alternative DesignsAlternative Designs Final DesignFinal Design Future WorkFuture Work

Problem StatementProblem Statement

ClientClient– An Evaluation of Carcinogenesis in a Zebrafish ModelAn Evaluation of Carcinogenesis in a Zebrafish Model

– Ultimate Goal: Cure CancerUltimate Goal: Cure Cancer

Design TeamDesign Team– Design a Zebrafish Holding ChamberDesign a Zebrafish Holding Chamber

– Compatible with a Micro CT ScannerCompatible with a Micro CT Scanner

– Sustains Zebrafish LifeSustains Zebrafish Life

Background InformationBackground Information CT = Computed TomographyCT = Computed Tomography

Benefits of Zebrafish ResearchBenefits of Zebrafish Research– Quick BreedersQuick Breeders– Low MaintenanceLow Maintenance– Mapped GenomeMapped Genome

Zebrafish AnatomyZebrafish Anatomy– Length: 1-6cmLength: 1-6cm– Lifespan: 2 yearsLifespan: 2 years– Water Temp: 64-75Water Temp: 64-75°° F F– Bony Fish: Breathe Without MovingBony Fish: Breathe Without Moving

Typical CT Scanner

Current Devices Current Devices

Market ProductsMarket Products– RodentsRodents– ZebrafishZebrafish

Client’s DeviceClient’s Device– CardboardCardboard– Zebrafish SlitsZebrafish Slits– High Mortality RateHigh Mortality Rate

Rodent Tray

Client’s Current Device

Preliminary ExperimentationPreliminary Experimentation February 11, 2004 ConclusionsFebruary 11, 2004 Conclusions

– Survival: 1.5 mL for >30 min Survival: 1.5 mL for >30 min – Increased Breathing Rates Increased Breathing Rates – Preferred OrientationPreferred Orientation– No Adverse Behavior in CuvetteNo Adverse Behavior in Cuvette– No Adverse Behavior with RestraintNo Adverse Behavior with Restraint

Design SpecificationsDesign Specifications

No leakageNo leakage

Zebrafish must remain Zebrafish must remain alive for 20 minutesalive for 20 minutes

Metal, glass, and dense Metal, glass, and dense materials must not be materials must not be usedused

Minimize all motion of the Minimize all motion of the zebrafishzebrafish

Disposable or Disposable or autoclavable autoclavable

Micro CT Scanner

Design 1: Falcon Tube Design 1: Falcon Tube

Falcon tube filled with Falcon tube filled with water with sponge water with sponge insideinside

Small slits to hold fishSmall slits to hold fish One to two fish per One to two fish per

tubetube Sponge absorptionSponge absorption

Side View of Plate in Container

3D View of Container – No Plates In Place

Water

Thumb – Screws

Design 2: Plexiglas ContainerDesign 2: Plexiglas Container

Multiple FishMultiple FishAdequate WaterAdequate WaterContaminationContamination

65mm

65mm

Design 3: Design 3: The CuvetteThe Cuvette

CuvetteCuvetteStabilization of FishStabilization of FishAlignment TrayAlignment TrayDisposable/InexpensiveDisposable/InexpensiveWater SupplyWater SupplySpace EfficientSpace Efficient

Criteria:Criteria: Cuvette Cuvette DesignDesign

Plexiglas Plexiglas ContainerContainer

Falcon Tube with Falcon Tube with SpongeSponge

CostCost 44 33 33

Ease of UseEase of Use 33 33 22

ConstructabilityConstructability 55 33 44

Effectiveness: Effectiveness: ImmobilizationImmobilization

44 44 33

Effectiveness:Effectiveness:

Survival RateSurvival Rate

55 55 44

TotalsTotals 2121 1818 1616

Design MatrixDesign Matrix

Each criterion was rated on a 0 to 5 scale with 5 Each criterion was rated on a 0 to 5 scale with 5 being the best.being the best.

Future WorkFuture Work

Cuvette TopsCuvette Tops– Rubber StopperRubber Stopper– MarketMarket

Sizing DifficultiesSizing Difficulties Opaque MaterialsOpaque Materials Build PrototypeBuild Prototype Prototype TestingPrototype Testing

Questions ?Questions ?