A’ utilities guide to starting up Anammox
Transcript of A’ utilities guide to starting up Anammox
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A’ utilities guide to starting up Anammox
Water NZ Conference 2018
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(Strous et al, 1998. Appl Microbiol Biotechnol 50: 589-596; Vlaeminck et al, 2010. Appli.Env.Microbiol 76, 900-909; Ni et al, 2013. BioMed Resch 469360;)
ANAMMOX (Anaerobic Ammonium Oxidation)
• It is a Microbe
• It is a Process • Discovered
in 1995
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THE ENERGY NEUTRALITY PROGRAMME
Mangere WWTP
Rosedale WWTP
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THE ENERGY NEUTRALITY PROGRAMME
Aeration reduction
Biochemistry optimization
Harvest more Carbon
Gas and biosolids
production
Higher capacity to climate
change adaptation
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BIOLOGICAL NITROGEN REMOVAL FROM WASTEWATER
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THE BOOM OF ANAMMOX TECHNOLOGY
Exponential use and study of anammox microbes for wastewater treatment
Full
scal
e in
stal
latio
ns
Scie
ntifi
c pu
blic
atio
ns
Year
(Lackner et al, 2015. Water Research 55, 292-303)
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THE BOOM OF ANAMMOX TECHNOLOGY
(Ali et al, 2015. Chemosphere 141, 144-153)
Bugs on a quest for world domination
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HOW SHALL WE START?
We need the bugs!
Develop our native strain
Import them from overseas
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1.Genetic detection
2.Enrichment
3.Scale-up
4.Pilot trials
5.Construction
6.Commissioning
NATIVE ANAMMOX IMPLEMENTATION PATHWAY
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1. DETECTION
Successful genetic detection in almost 60 wastewater samples Presence at very low concentrations Two identified species
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2. ENRICHMENT
Three wastewater samples each cultivated in a SBR bioreactor (3L) • Mangere RAS (R) • Rosedale RAS (M) • Digesters sludge (D)
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2. ENRICHMENT
Cultivation time (days)
Amm
oniu
m c
once
ntra
tion
(mg-
N L
-1)
Positive results (NH4
+ consumption) for all three reactors after 250 days of cultivation
Rosedale RAS (R)
Mangere RAS (M)
Digesters sludge (D)
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• Increasing anammox genes
• Increasing activity rates
• Nitrogen gas production
• By-product (NO3-) formation
2. ENRICHMENT
Further evidence of successful anammox enrichment
NH
4+ con
sum
ptio
n ra
re
(mg-
N L
-1 d
-1)
Cultivation time (day)
(R) (M) (D)
Anam
mox
gen
e co
pies
Lo
g10 (
copi
es/u
L)
Cultivation time (day)
N2 gas bubbles
NO
3- con
.(mg-
N L
-1)
Cultivation time (days)
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2. ENRICHMENT
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3. SCALE-UP
Humbly, but putting New Zealand back on the map
Amm
oniu
m v
olum
etric
load
ing
rate
(K
g-N
m-3
d-1
)
Anammox based BNR systems
(Third et al, 2005. Microbial Ecol 49:236-244; Lackner et al, 2015. Water Research 55, 292-303)
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Scale-up strategy On-going work.
3. SCALE-UP
3 L 15 L 250 L 1250 L50 L
12 months
12 months
12 months
12 months
Centrate pre-treatment
Centrate
Suspended sludge
Carriers (MBBR)
Lab scale Scale up Pilot scale
Overseas strain(Austria)
2 X 15 L
PC2 containment
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• We now have cultures which will be used to seed larger reactors
• We developed a method to enrich anammox bugs which has resulted in valuable knowledge and potential Intellectual Property
• We have built an on-site Wastewater Innovation Centre, available to do pilot trials
PROMISING FUNDATIONS
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• It has been a very valuable and challenging journey
• Anammox based BNR technology is now reachable in N.Z.
• On target to achieve Energy Neutrality by 2025
• Our operations and partnerships will intensify to really reach this goal
SUMMARY
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Thanks