A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis...
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Transcript of A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis...
![Page 1: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/1.jpg)
A sensor histidine kinase coordinates cell wall architecture
with cell division in Bacillus subtilis
Component annotationPMID:18573169
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574549/
![Page 2: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/2.jpg)
Simple component annotation
• Figure 1 localizes 2 proteins to the septum of B. subtilis
![Page 3: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/3.jpg)
What proteins?
![Page 4: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/4.jpg)
What GO term?
• “YycG and FtsZ co-localize to the septum”
• Septum can be at the cellular level or at the level of a tissue
• Qualifier: colocalizes_with
![Page 5: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/5.jpg)
Evidence
• Experimental - Yes– Mutant – No– Based on direct
physical interaction – No
– Direct assay – Yes• IDA
![Page 6: A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis Component annotation PMID:18573169 .](https://reader035.fdocuments.us/reader035/viewer/2022062217/5697c02a1a28abf838cd822d/html5/thumbnails/6.jpg)
Additional annotations
• Figures 2-4 include evidence for annotations that are not allowed in CACAO (protein binding)
• Figure 5 supports annotation of another protein, YycF to GO:0009295 ! Nucleoid via similar localization experiments
• Figure 6 supports other annotations