Slide 1

A Review of the 2014 Mark Procedures Manual Tiffani Marsh 1, Charles Morrill 2, Pat Keniry 3, Stephen Pastor 4, Jeff Fryer 5, Scott Putnam 6, Brandon Chockley 7 1. NOAA Fisheries; tiffani.marsh@noaa.gov 2. Washington Department of Fish & Wildlife 3. Oregon Department of Fish & Wildlife 4. U.S. Fish and Wildlife Service 5. Columbia River Intertribal Fish Commission 6. Idaho Department of Fish & Game 7. Fish Passage Center Slide 2 Outline 1.Purpose of the manual Slide 3 Outline 1.Purpose of the manual 2.Special Notices Slide 4 Outline 1.Purpose of the manual 2.Special Notices 3.Review of the various sections Slide 5 Outline 1.Purpose of the manual 2.Special Notices 3.Review of the various sections 4.Questions Slide 6 Purpose As stated in the document This PIT Tag Marking Procedures Manual provides protocols and standards on the PIT tag marking station(s), fish handling techniques, anesthesia, tag injection, and information on data collection, verification, and transfer to the regional database. In addition to this manual, the PTSC and PTAGIS produced a PIT tag training video that is available from PTAGIS. If you have specific questions that are not answered by this document or the video, or if you would like to receive training, please contact a PTSC representative for assistance. Slide 7 Purpose As stated in the document This PIT Tag Marking Procedures Manual provides protocols and standards on the PIT tag marking station(s), fish handling techniques, anesthesia, tag injection, and information on data collection, verification, and transfer to the regional database. In addition to this manual, the PTSC and PTAGIS produced a PIT tag training video that is available from PTAGIS. If you have specific questions that are not answered by this document or the video, or if you would like to receive training, please contact a PTSC representative for assistance. PIT Tag Specification Document Slide 8 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. Slide 9 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Slide 10 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Slide 11 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag Slide 12 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature Slide 13 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size Slide 14 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size Juvenile salmonid tagging Slide 15 Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size Juvenile salmonid tagging It is understood that some research studies will not be able to follow some of the protocols. Slide 16 Slide 17 Special Notices Since July 1992, only disinfected injectors and tags have been approved for use in the Columbia River Basin for all hatchery and wild anadromous fish. This requirement eliminated the use of semiautomatic injectors. Disinfection using standard hand held injectors requires immersing the needle, syringe, and the end of the injector push rod in 70-80% ethyl alcohol or 60-80% isopropyl alcohol for a period of not less than 10 minutes. Currently, there is a single-use injector system (SUI) available to which the disinfection requirement does not apply, as long as the needle assembly used in this system is not re-used. If a tagger wishes to re-use the single-use needle assembly, disinfection between fish is required. Slide 18 Special Notices Since July 1992, only disinfected injectors and tags have been approved for use in the Columbia River Basin for all hatchery and wild anadromous fish. This requirement eliminated the use of semiautomatic injectors. Disinfection using standard hand held injectors requires immersing the needle, syringe, and the end of the injector push rod in 70-80% ethyl alcohol or 60-80% isopropyl alcohol for a period of not less than 10 minutes. Currently, there is a single-use injector system (SUI) available to which the disinfection requirement does not apply, as long as the needle assembly used in this system is not re-used. If a tagger wishes to re-use the single-use needle assembly, disinfection between fish is required. Slide 19 Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption. Slide 20 Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption. Fisherman in the lower river dorsal sinus Slide 21 Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption. Fisherman in the lower river dorsal sinus Idaho Statesman article, A pit tag is crunchy, but not tasty, 11 March 2014 Slide 22 Contents Slide 23 1.Tagging stations Slide 24 Contents 1.Tagging stations 2.Tagging operations Slide 25 Mass marking Slide 26 Slide 27 Traps and weirs Slide 28 Streamside Slide 29 Contents 3.Fish handling techniques Slide 30 Contents 3.Fish handling techniques Minimizing Fish Stress Slide 31 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Slide 32 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Slide 33 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia Slide 34 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization Slide 35 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization 4.Fish Recovery and Release Slide 36 Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization 4.Fish Recovery and Release 5.Post-Tagging Mortality and Tag Retention Slide 37 Contents 6.PIT Tag Injectors Slide 38 Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Slide 39 Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Slide 40 Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction Slide 41 Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction Single use injectors (SUI) Slide 42 Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction Single use injectors (SUI) Exempt from disinfection, unless re-used Slide 43 Contents 7.Tag Injection Slide 44 Holding the fish Slide 45 Holding the injector Slide 46 Tag insertion Slide 47 Tag placement Slide 48 Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size Slide 49 Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size 65mm or larger Slide 50 Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size 65mm or larger 8.Computerized Data Entry Slide 51 Possible additions to document Guidelines Smaller tags Tagging adult salmonids Tagging non-salmonids Additional anesthetics Slide 52 Web links www.ptagis.org PTAGIS www.ptagis.org http://www.ptagis.org/resources/document- library - this manual and 2009 Specification Doc http://www.ptagis.org/resources/document- library www.monitoringmethods.org BPA https://www.monitoringmethods.org/Method/ Details/1736 - Chris Tatara https://www.monitoringmethods.org/Method/ Details/1736 https://www.monitoringmethods.org/Method/ Details/4146 - Tiffani Marsh https://www.monitoringmethods.org/Method/ Details/4146 Slide 53 Questions?