A new method to measure 10 B uptake in lung adenocarcinoma in hospital BNCT

A new method to measure 10B uptake in lung adenocarcinoma in hospital BNCT

D. Bolognini, E.M. Donegani, A. Mattera, M. Prest, S. Hasan

E. Capelli, P. Chiari

A. Zanini

G. Giannini, E. Vallazza

F. Basilico, P. Mauri

F. BorasioP. Cappelletti, M. Frigerio, S. Gelosa, A. F. Monti

BNCT

The INFN PhoNeS project

An innovative approach to Boron Imaging

Neutron Autoradiography

Measurements and results

Conclusion

Outline

Boron Neutron Capture Therapy

BNCT could become useful for highly-fatal tumours:

•Extended (e.g. liver, lung)•Radioresistant (melanoma)•Localized near vital organs

(glioblastoma)

10B (n,α,γ) 7Libinary technique high LET particles

High intensity thermal-epithermal

neutron beam(5·108 n/cm2 s1)

< 10 keV energy range

Gamma contamination < 2 ·10-13 Gy cm2 / n

Low toxicity

High specificity

Non-persistence in the bloodstream

BSH

BPA

Difficulties for BNCT applications

Neutron Autoradiography

Nuclear reactor Solid state track detector (CR 39) Sometimes cryogenic temperature during the irradiation Optic microscope for identification and count

Nuclear reactor Solid state track detector (CR 39) Sometimes cryogenic temperature during the irradiation Optic microscope for identification and count

Hospital-based neutron source Real time detector

Hospital-based neutron source Real time detector

PhoNeS (PhotoNeutronSource)Neutron flux in the treatment cavity: Φ = (1.3 ± 0.1) x 107 n cm-2 s-1

γ-contamination: reduced of 97%

Real-time Neutron Autoradiography

0.5 cm CERROBEND

photonconversion

6 cm PMMA

thermalization

IRON BOX

•Samples

•DetectorNeutron flux: Φ = (1.5 ± 0.1) x 105 n cm-2 s-1

The imaging detector

Silicon Dimensions (9.5 x 9.5) cm2

Hamamatsu Microstrip Silicon Detector 768 strips

Readout pitch 242 μm

Read-out by 3 self-triggering TAA1 ASICs

Counts vs position

The Data Acquisition System

• INTER-BUNCH acquisition

• UNDEPLETED silicon detector

to detect α-particles from boronated samples down to 25 ng of 10B

18 MeV electrons

Bunches of 5 μs

Frequency 100 - 300 Hz

SPECIMEN SUPPORT

-plastic support (liquid solution)evaporation

-glass slide (blood samples)coagulation process

to prevent crystallizationand self-absorption ofthe α-particles by the sample itself

KNOWN VOLUME

Drops of 5 μl

The samples

SOLUTION HOMOGENEITY

Calibrating with BSH

500 MU/min –18 MV photons Varian CLINAC 2100 C/D, Sant’Anna Hospital (Como)

Integrated Spot Content (AU) vs Concentration (ppm)

The first ever kinetic curves

Special thanks to Dr.Sauerwein

URINEBLOOD

rescaled at the max value obtained by Si-autoradiography

Blood and urine of a patient administered BSH, taken at different times with respect to the administration.

Ex-vivo lung perfusion

Human lung affected by adenocarcinoma:

• therapetic resection

• perfusion of blood and BPA

Blood samples taken at different times:

• to estimate 10B uptake

in collaboration with “S.Luigi” Hospital in Orbassano (TO), Italy

Kinetic curves

• Blood samples doped with BPA

• Comparison between real-time autoradiography to MS analysis

• The absorption takes place only in the first 90mins

CR 39

Healthy tissue

Unhealthy tissue

Special thanks to Dr. Giannini

10B concentration in tumour and healthy tissues of resected lobe

High-resolution images to reproduce the features

of the tissues

CT / CH ~ 1.5 - 2

Conclusions

Neutron Autoradiography in a hospital environment successfully performed - Linac

Development of an hospital-base neutron source to perform Boron Imaging with a 1D spatial resolution

Real-time detector to perform pharmacokinetic studies results during the irradiation

Blood samples from a perfused lung affected by adenocarcinoma kinetic curves - 10BPA down to concentrations of 5ppm

Evaluation of 10B concentration in tumour and healthy tissues Si-autoradiography – CR 39

Looking forward to make BNCT possible in every radiotherapic unit

A new method to measure 10B uptake in lung adenocarcinoma in hospital BNCT

D. Bolognini, E.M. Donegani, A. Mattera, M. Prest, S. Hasan

E. Capelli, P. ChiariA. Zanini

G. Giannini, E. Vallazza

F. Basilico, P. Mauri

F. BorasioP. Cappelletti, M. Frigerio, S. Gelosa, A. F. Monti

Thank you!

A new method to measure 10 B uptake in lung adenocarcinoma in hospital BNCT

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